Search Results (2,084)

Introduction: Marine heatwaves are increasing in frequency and intensity, posing major challenges for fishes inhabiting shallow coastal ecosystems. Short-term exposure to extreme warming can alter metabolic performance and thermal tolerance, with potential consequences for species persistence and school composition in thermally variable habitats. Understanding the capacity of coastal fishes to withstand acute warming events is therefore essential for predicting ecological responses to climate change. Objective: We aimed to determine the effects of simulated marine heatwaves on thermal tolerance and metabolic performance in juvenile grey mullets, Chelon labrosus and Chelon aurata, two abundant sympatric species inhabiting the Ria Formosa lagoon (southern Portugal). Methodology: Juvenile mullets acclimated at 17 °C were exposed to simulated heatwave treatments of 23, 27, or 33 °C and sampled either at peak temperature or after 48 h and 1-week recovery at 17 °C. Critical thermal maximum (CTmax, using a 1 °C/min thermal ramp), static oxygen consumption (MO2), and intermittent respirometry parameters were measured. Standard metabolic rate (SMR), maximum metabolic rate (MMR), and aerobic scope (AS) were derived from intermittent respirometry. A complementary temperature-ramp (>3 h at each temperature step 17, 23, 27 and 33 °C) was performed to evaluate routine metabolic rate and estimate Q10 values across increasing temperatures. Additional plasma and tissue analyses are being conducted to assess energetic substrate mobilization and cellular responses to thermal and oxidative stress. Results: CTmax increased significantly with warming in both treatment modes, demonstrating rapid heat hardening in juvenile mullets. Fish exposed to 27 and 33 °C exhibited higher CTmax than control fish, and this elevated tolerance persisted after recovery. Chelon labrosus showed slightly higher CTmax values than C. aurata. Oxygen consumption increased with temperature, with the strongest responses occurring at 33 °C. SMR increased markedly with warming, particularly in heatwave-exposed fish, while MMR increased mainly at the highest temperature treatment. In contrast, AS showed no clear thermal optimum or decline across treatments. Routine metabolic rate increased non-linearly with temperature in the complementary ramp experiment, with a mean Q10 of 2.28, confirming strong thermal dependence of metabolism. Conclusions: Juvenile mullets possess substantial short-term thermal plasticity and can rapidly increase heat tolerance during marine heatwaves but this enhanced tolerance is accompanied by elevated metabolic costs under extreme warming, indicating potential energetic trade-offs near upper thermal limits. Differential physiological responses between species may influence school composition and ecological performance across thermal landscapes. Ongoing plasma and tissue analyses will further clarify the energetic and cellular mechanisms underlying thermal and oxidative stress resilience in coastal fishes. Full article

Background/Objective: Caffeine has demonstrated ergogenic effects across various doses (2–9 mg·kg⁻¹). However, aerobic responses to caffeine vary substantially, with time-trial performance ranging from ~–3% to +16%. Given that higher doses may increase adverse effects without clear additional benefits, this review examined the effects of low (≤3 mg·kg⁻¹), moderate (4–6 mg·kg⁻¹), and high (>6 mg·kg⁻¹) caffeine doses on time-trial performance. Methods: A systematic review and meta-analysis of randomized, placebo-controlled trials was conducted using PubMed, Embase, and Virtual Health Library databases. Eligible studies included healthy adults (18–59 years) acutely ingesting oral anhydrous caffeine before aerobic time-trial tests, with performance outcomes measured exclusively as time-to-completion variables. Data were pooled using standardized mean differences (SMDs) and 95% confidence intervals under random-effects models, and risk of bias was assessed using the Cochrane Risk of Bias tool. Results: Forty-eight studies (689 participants) met the inclusion criteria. Both low and moderate caffeine doses significantly reduced time-trial completion time relative to placebo. Low doses produced a standardized mean difference of −0.27 (95% CI: −0.44 to −0.11; p = 0.001), whereas moderate doses resulted in an SMD of −0.52 (95% CI: −0.77 to −0.28; p < 0.0001). No studies evaluating high caffeine doses (>6 mg·kg⁻¹) and reporting time-to-completion outcomes met the inclusion criteria. Subgroup analyses demonstrated similar ergogenic effects in both trained and highly trained individuals consuming moderate caffeine doses. Conclusions: This is the first meta-analysis specifically focused on aerobic time-trial performance to suggest that pre-exercise ingestion of low caffeine doses (1.3–3 mg·kg⁻¹) may enhance endurance performance by reducing time-trial completion time. Notably, the use of moderate caffeine doses (4–6 mg·kg⁻¹) appears to produce a more consistent ergogenic effect. Full article

Objective: Sodium–glucose cotransporter 2 (SGLT2) inhibitors, particularly empagliflozin, have attracted considerable attention because of their cardiovascular benefits beyond glycemic control. However, the interaction between empagliflozin and exercise-induced physiological cardiac remodeling in healthy individuals remains insufficiently understood. This study investigated the effects of different swimming exercise protocols (anaerobic, aerobic, and endurance), administered alone or in combination with empagliflozin, on cardiac structure and electrophysiology. Methods: Thirty-six male Sprague–Dawley rats were randomly assigned to six groups (n = 6 per group): anaerobic (An), aerobic (Ae), endurance (En), and the corresponding exercise groups combined with empagliflozin (An + Empa, Ae + Empa, and En + Empa). Empagliflozin was administered by oral gavage at a dose of 15 mg/kg/day for 30 days. Transthoracic echocardiography, electrocardiography (ECG), and gastrocnemius electromyography were performed at baseline and at the end of the study to assess cardiac remodeling, heart rate, and neuromuscular function. The study was carried out over a 30-day intervention period following ethics committee approval on 24 July 2024. Results: No significant between-group differences were observed in echocardiographic parameters before the intervention. On day 30, significant differences were identified among the groups in interventricular septal thickness at end-diastole (IVSd) (p = 0.027), left ventricular internal diameter at end-diastole (LVIDd) (p = 0.009), and end-diastolic volume (EDV) (p = 0.014). Bonferroni-corrected post hoc analysis showed that the aerobic exercise plus empagliflozin group differed from several exercise-only groups, particularly in parameters related to ventricular size and filling volume, including LVIDd and EDV (p < 0.008). On day 30, electrocardiographic repolarization-related parameters, including QT, QTc, JT, and Tpeak–Tend intervals, also differed significantly among the groups (all p < 0.05). In post hoc analysis, the anaerobic exercise group showed significant differences in QT and JT intervals compared with the aerobic and endurance groups (p < 0.008). In the anaerobic protocol, empagliflozin was associated with a reduction in heart rate compared with the corresponding control group (p = 0.019). No significant between-group differences were observed in EMG findings. Conclusions: Different exercise protocols induce distinct patterns of adaptation in cardiac structure and electrophysiology in healthy rats. Empagliflozin (15 mg/kg/day) may modulate exercise-induced cardiac responses in a modality-dependent manner; the most pronounced echocardiographic effects were observed in the aerobic protocol, whereas the effect on heart rate was observed in the anaerobic protocol. These findings highlight the need for longer-term and mechanistic studies to further clarify the effects of SGLT2 inhibitors on physiological cardiac remodeling. Full article

Youth soccer performance is influenced by multiple factors, including age, body size, and physical capacities, but the relative contribution of these variables to aerobic and explosive performance remains unclear. Understanding these relationships can improve the interpretation of field tests and support individualized training prescription. This study was designed to examine the association of age category, body mass, and height with physical performance in youth soccer players by jointly considering aerobic and explosive capacities, in order to support the interpretation of field tests within training prescription. Forty-five male players (15 U16, 15 U17, 15 U19) from the same club were assessed across two standardised on-field testing sessions, including the 45–15 test (estimated maximal aerobic speed, MAS) and vertical jump tests (squat jump, SJ; countermovement jump, CMJ; countermovement jump with free arms, CMJ_FH). Performance variables (SJ, CMJ, CMJ_FH, MAS) were treated as outcomes, while category, body mass, and height were included as predictors. A multivariate analysis was performed, followed by univariate analyses for each indicator. Results showed a significant multivariate effect of age category on overall performance (p < 0.001; η²p = 0.482), whereas height and body mass were not significant (p > 0.05). In univariate analyses, age category was associated with all variables: SJ (p = 0.005; adj. R² = 0.160), CMJ (p < 0.001; adj. R² = 0.287), CMJ_FH (p = 0.004; adj. R² = 0.173), and MAS (p < 0.001; adj. R² = 0.352). Performance increased progressively from U16 to U17 to U19, with larger between-category differences in aerobic capacity. In conclusion, age category was more strongly associated with the performance profile than height and body mass when considered jointly; these findings should be interpreted in light of the observational design and the lack of biological maturation measures. Full article

Background: Exercise training is known to improve metabolic health in individuals with obesity; however, its role in facilitating transitions between metabolically unhealthy obesity (MUO) and metabolically healthy obesity (MHO), as well as the associated metabolic adaptations, remains incompletely understood. Methods: A total of 84 young adults with obesity were enrolled and classified MUO (n = 55) or MHO (n = 29) based on baseline metabolic profiles. All participants completed an 8-week supervised exercise intervention. Anthropometric parameters, body composition, cardiometabolic markers, and VO₂max were assessed before and after the intervention. Targeted metabolomics of 30 amino acid-related metabolites was performed in the MHO-R (remained MHO) and MUO-C (conversion from MUO to MHO) groups to explore exercise-associated metabolic adaptations following intervention. Results: Exercise training improved cardiometabolic risk profiles, including reductions in adiposity and improvements in insulin resistance-related markers. A proportion of participants transitioned from metabolically unhealthy to metabolically healthy obesity following the intervention. No significant between-group differences in amino acid metabolite changes were observed between MHO-R and MUO-C groups. Exploratory metabolomic analyses identified exercise-responsive alterations in amino acid-related metabolites, particularly involving arginine biosynthesis and histidine metabolism. Conclusions: Combined aerobic and resistance training is associated with improvements in metabolic health and phenotype transition in young adults with obesity. Observed alterations in arginine and histidine metabolism may reflect metabolic adaptations to exercise rather than transition-specific or causal mechanisms underlying phenotype conversion. Full article

Introduction: Coastal fishes can adapt to water warming and hypoxia; however, acute tolerance does not necessarily predict longer-term performance and survival. This may be especially important in sedentary, site-faithful species with limited escape to escape increasingly unfavorable habitats. We assessed the climate-related stress responses of the Lusitanian toadfish, Halobatrachus didactylus, a benthic estuarine fish from the Northeast Atlantic, to water warming and hypoxia. Objectives: We aimed to determine the aerobic energy budget, thermal limits (CTmax), and critical oxygen tension (Pcrit), as well as blood indicators of metabolism, altered physiology and systemic stress, as proxies for whole-organism homeostatic state, thereby informing future ecophysiological assessments and bioindicator development in a context of environmental change. Methodology: We determined standard, routine, and maximum metabolic rates; aerobic scope; and critical thermal maximum (CTmax) and critical oxygen (Pcrit) thresholds on a set of 134 individuals ranging from 12 to 160 g in weight. On a different set of individuals (n = 48; 76.3 ± 2.6 g; 16.1 ± 0.18 cm), we simulated 30 days of seasonal scenarios combining low and high temperature with normoxia or hypoxia, followed by integrated metabolic, hematological, biochemical, and multivariate analyses. Results: Acute trials showed high short-term resilience: H. didactylus had an exceptionally low standard metabolic rate and routine metabolic rate, high CTmax (34.82 ± 0.66 °C), and strong hypoxia tolerance (Pcrit 0.59–1.97 mg O₂ L⁻¹), although smaller individuals were more sensitive. After 30 days, however, warming more than doubled standard and routine metabolic rates, while warm hypoxia reduced metabolic output relative to warm normoxia, consistent with metabolic depression under compounded stressors. This treatment also showed shifts in glucose, liver mass, red blood cell count, and hematocrit, identifying warm, oxygen-poor water as the most physiologically costly scenario for this species. Conclusions: Together, these results show that high acute tolerance does not guarantee resilience to climate change. In sedentary fishes, survival may depend less on surviving extremes than on maintaining energetic balance, oxygen transport capacity, and physiological homeostasis in increasingly warm, oxygen-poor coastal habitats. Full article

Introduction: Climate warming and drought are intensifying thermal stress in Mediterranean freshwater systems, potentially favoring invasive fish with broad physiological tolerance. Extended environmental tolerance and increased aerobic scope are indicative of the potential to sustain, perform and disseminate in challenging conditions. Objective: We aimed to determine the thermal scope of the invasive Australoheros facetus inhabiting southern Portuguese drainages using an array of physiological proxies. Methodology: We evaluated the thermal biology of the species across a wide temperature gradient to test how warming affects metabolic performance, thermal tolerance, and biochemical status. Fish collected from Algarve watercourses were exposed to 5, 10, 15, 20, 25 and 35 °C (n = 15 per condition, 10–60 g) for at least a week, and intermittent respirometry was used to determine standard metabolic rate (SMR), maximum metabolic rate (MMR) and aerobic scope (AS). Group Q10 was derived from metabolic rates. Plasma and tissue biomarkers of energy metabolism and oxidative stress were analyzed. Critical thermal maximum (CTmax) was assessed in fish acclimated for a week at 10, 20 and 30 °C (n = 10) using a 1 °C/min thermal ramp. Results: Intermediate temperatures (15–25 °C) supported the best overall physiological performance, combining stronger aerobic capacity with higher antioxidant protection. In contrast, 30–35 °C imposed clear physiological costs: maintenance metabolism increased disproportionately, aerobic scope declined, and cellular protection weakened, indicating the onset of heat stress. Despite this, A. facetus showed marked thermal plasticity, with CTmax increasing significantly with acclimation temperature. Fish acclimated to 30 °C had higher CTmax than fish acclimated to 20 °C and 10 °C, although the thermal safety margin decreased progressively as the acclimation temperature rose. Liver antioxidant activity also peaked at intermediate temperatures and declined at the warmest treatments, reinforcing the mismatch between acute tolerance and sustained performance. Conclusions: These results show that A. facetus is highly heat tolerant but that tolerance comes with energetic and cellular trade-offs near upper thermal limits. Despite this limitation at extreme conditions, the combination of broad tolerance and functional performance under warm intermediate conditions may help to explain its invasion success and stand as a competitive advantage in increasingly hot low-flow Iberian freshwater ecosystems. Full article

Back-mixing has been widely applied during practical composting to initiate the process and improve compost product quality. However, for antibiotic mycelial residue (AMR), a fermentation by-product containing residual antibiotics, the ecological safety of this treatment remains unclear. In this study, pleuromutilin mycelial residue (PMR) was subjected to a 35-day aerobic composting experiment with a back-mixing treatment (T group) and the conventional composting group (CK group) to evaluate composting performance and antibiotic resistance risk. The results demonstrated that the T group exhibited more rapid heating and a higher degree of humification. Additionally, the T group not only exhibited faster pleuromutilin degradation, reaching below the detection limit 3 days earlier than in the CK group, but also achieved up to a 3.1-fold reduction in antibiotic resistance genes (ARGs) and a 93.2% overall reduction in mobile genetic elements (MGEs). Redundancy analysis (RDA), variance partitioning analysis (VPA), and co-occurrence network analysis indicated that microbial community structure appeared to be more strongly associated with ARG variation than MGEs under the tested conditions. Overall, back-mixing accelerated pleuromutilin degradation and enhanced PMR composting performance, while no substantial enrichment of the detected ARGs was observed under the tested composting conditions. This study provides a scientific basis for the safe resource utilization of AMR. Full article

Breast cancer is the most prevalent malignancy among women. The disease and its treatments often lead to physical and psychosocial impairments, compromising quality of life. While exercise and music-based interventions have individually demonstrated benefits on these symptoms, the advantages of their combination remain unexplored. This study evaluates whether a concurrent exercise program, including aerobic and strength training performed while listening to music based on individual preferences, is more effective than the same exercise program without music in improving self-esteem, body image, cancer-related fatigue, physical function (upper and lower limb strength and cardiorespiratory endurance), quality of life, sleep quality, and intolerance of uncertainty in female breast cancer survivors. A single-blind, two-arm, parallel-group, randomized controlled trial will be conducted including 42 women who completed primary treatment for stage 0–III breast cancer at least six months before enrollment. Participants will be randomly assigned to a music-listening concurrent exercise training group (MLTG), or a no music exercise training group (NMLTG), both performing the same 8-week exercise program. The primary outcome will be self-esteem (Rosenberg Self-Esteem Scale). Secondary outcomes include body image, fatigue, upper and lower limb strength, cardiovascular endurance, quality of life, sleep quality, and intolerance of uncertainty. Measurements will be collected using validated and reliable questionnaires and standardized functional tests at baseline, post-intervention, and at 6 months. Data will be analyzed under the intention-to-treat principle. Music listening, particularly when based on individual preferences and synchronized with movement, may enhance mood and exercise performance while modulating reward-related neural pathways. This trial will provide new evidence on a feasible and low-cost strategy to enhance supportive care and physical and psychosocial outcomes in breast cancer survivors. ClinicalTrials.gov (NCT07045961). Ethics Committee code: 2025-0855. Full article

The horse represents one of the most physiologically specialized athletic mammals, capable of sustaining both high-intensity and prolonged exercise. Central to this remarkable performance capacity is the metabolic adaptability of skeletal muscle and its mitochondrial network. This narrative review synthesizes current evidence from equine, human, and rodent studies on exercise-induced mitochondrial remodeling in equine skeletal muscle. A comprehensive literature search was conducted across PubMed, Web of Science, and Scopus using terms related to equine exercise physiology, mitochondrial biology, and skeletal muscle metabolism. Preference was given to peer-reviewed original research and review articles. Mitochondria regulate oxidative phosphorylation, substrate oxidation, redox signaling, and cellular responses to metabolic stress induced by exercise. Training induces extensive mitochondrial adaptations, including mitochondrial biogenesis, remodeling of the respiratory chain, enhanced oxidative phosphorylation efficiency, and increased metabolic flexibility. These adaptations are believed to contribute to improvements in aerobic capacity, delayed fatigue onset, and enhanced recovery following exercise, although direct mechanistic evidence in horses remains limited. In equine skeletal muscle, mitochondrial plasticity is closely linked to muscle fiber composition and the distribution of oxidative and glycolytic fibers. Exercise-induced signaling pathways involving AMP-activated protein kinase (AMPK), Ca²⁺-dependent kinases, and the transcriptional coactivator PGC-1α regulate mitochondrial biogenesis and metabolic remodeling. In addition, mitochondrial dynamics, including fusion, fission, and mitophagy, maintain mitochondrial quality and functional efficiency during repeated training stimuli. Experimental studies in Thoroughbred and Standardbred horses demonstrate that training has been associated with increases in mitochondrial density and respiratory capacity in equine skeletal muscle, contributing directly to improved aerobic performance and metabolic efficiency. However, mitochondrial adaptations must be interpreted within the broader context of musculoskeletal adaptation, as metabolic improvements may occur faster than structural adaptation of tendons and ligaments. This review synthesizes current knowledge on exercise-induced mitochondrial remodeling in equine skeletal muscle, while highlighting the limited mechanistic evidence available in horses and the need for more standardized longitudinal studies. Full article

Background/Objectives: Oral and maxillofacial infections present polybacterial profiles, including both aerobic and anaerobic bacteria. Increasing antibiotic resistance poses a significant challenge to pharmacological treatment of these infections. The aim of this study was to present a bacterial profile and assess antibiotic resistance found in these infections. Methods: This retrospective analysis is based on medical records of 224 patients affected with maxillofacial infections. Microbiological cultures and antibiotic susceptibility testing were performed for all patients. Results: In 78.57% of the patients, a positive microbiological culture was obtained. A total of 72.72% of culture-positive patients showed multi-bacterial cultures (128/176). Predominant bacteria included Streptococcus, detected in 156 cases (39%), followed by Staphylococcus, found in 64 cases (16%), and Prevotella, detected in 56 of 400 total bacterial isolates (14%). The most often isolated aerobic strains were Streptococcus mitis/oralis detected in 64 (16%) cases and Staphylococcus epidermidis detected in 48 cases (12%), while the most common anaerobic strains were Prevotella buccae detected in 14 cases (3.5%). Streptococcus and Staphylococcus exhibited the greatest resistance to clindamycin, accounting for 51.74% and 47.63%, respectively. Aerobic Gram-positive cocci were more resistant to penicillin and amoxicillin than to cephalosporins. Among obligate anaerobes, the lowest antibiotic resistance seen was to metronidazole. The obligate anaerobes except Prevotella were sensitive to clindamycin. Conclusions: A high rate of clindamycin resistance among aerobic and facultatively anaerobic Gram-positive cocci indicates the need to reassess the use of clindamycin in empirical therapy. The bacterial composition of infections suggests the need to use combined antibiotic therapy. First- and second-generation cephalosporins may be an effective alternative to penicillin and its derivatives. Full article

Soccer is a complex sport with significant physical, physiological, psychological, technical, and tactical demands on players. This review presents an ergonomics-based model of soccer performance, emphasizing that no single component operates in isolation. Building on the foundational ergonomic framework, this review integrates contemporary evidence on training load monitoring, ecological dynamics, and cognitive-perceptual performance dimensions not systematically addressed in prior frameworks. Elite outfield players cover 9–14 km·h⁻¹ per match, with high-speed running (19.8–24.8 km·h⁻¹) making up about 20% of total distance and sprinting (>25 km·h⁻¹) around 2%. These outputs vary by playing position, tactical formation, possession dynamics, and environmental conditions. Longitudinal data from the English Premier League indicate a 35% increase in high-speed running over the past decade, suggesting intensifying physical demands. Physiological responses, including average heart rates of 156–175 bpm, reflect the aerobic and anaerobic demands on players. The review also examines benchmarks like VO₂max, sprint velocity, and anthropometry, highlighting their utility and limitations as performance indicators. Regarding training load management, the review evaluates frameworks such as the Acute:Chronic ratio and high-speed running exposure protocols, noting limitations and risks of over-relying on external load metrics. Periodization approaches, including tactical periodization, are discussed for integrating physical, technical, tactical, and psychological components in training. The proposed ergonomic model conceptualizes elite soccer performance as an emergent property of interacting physical, physiological, tactical, psychological, and environmental subsystems, with direct implications for training design, selection, and load management. Selection decisions should consider cognitive and perceptual competencies like decision-making, anticipation, and situational awareness, alongside physical and physiological profiles, aligned with the team’s game model. Full article

Background/Objectives: Evidence for herbal ergogenic aids remains uncertain, and ashwagandha trials span heterogeneous performance domains. This review evaluated oral Withania somnifera supplementation on exercise performance and explored participant-, outcome-, formulation-, and supplementation-related moderators. Methods: PubMed, Web of Science, Cochrane Library, Embase, and SPORTDiscus-EBSCO were searched from inception to 1 April 2026. Eligible randomized controlled trials compared oral ashwagandha with placebo or control conditions and reported objective exercise-performance outcomes. Dependent effects were synthesized using restricted-maximum-likelihood three-level random-effects models; 95% prediction intervals, GRADE certainty ratings, subgroup analyses, and dose/duration meta-regressions were reported. Results: Thirteen trials involving 599 participants contributed 79 effect sizes. Samples were mainly young adults or athletes; reported ages included one 18–40-year trial and one late-adolescent athlete cohort aged 17.4 ± 1.7 years. Trial-level sex composition was four male-only, one female-only, three mixed-sex, and five incompletely reported cohorts. Ashwagandha improved overall exercise performance on average (Hedges’ g = 0.47, 95% CI [0.25, 0.69], p < 0.001; I² = 60%; 95% prediction interval [−0.40, 1.33]), but the prediction interval crossed zero. Exercise type was the clearest moderator (P_between = 0.006): evidence was most consistent for aerobic endurance (g = 0.54, 95% CI [0.22, 0.85], p = 0.002), whereas strength effects were positive but uncertain and power or muscular endurance evidence remained sparse. Dose analyses were hypothesis-generating; 500–600 mg/day was the most evidence-supported extract-dose range. Conclusions: Oral ashwagandha may improve selected exercise-performance outcomes, particularly aerobic endurance, but benefits are not uniform across contexts. Future trials should be preregistered, adequately powered, double-blind, formulation-standardized, sex-stratified, and include rigorous blinding checks, mechanistic endpoints, adverse-event monitoring, and sport-specific performance tests. Full article

The efficacy of plant-derived antimicrobials in meat systems is frequently limited by interactions with proteins, lipids, and other food matrix components that reduce the bioavailability and antimicrobial activity of phytochemicals. This study evaluated the antimicrobial effectiveness of Verbascum sinaiticum (V. sinaiticum) leaf extract encapsulated using maltodextrin (MD), gum arabic (GA), and a maltodextrin–gum arabic blend (MDGA, 8:2 w/w) through freeze-drying for application in raw beef during refrigerated storage (4 °C). The encapsulation systems exhibited process yields of 42.5–54.7%, encapsulation efficiencies of 78.3–92.5%, and loading capacities of 18.5–24.3 mg GAE/g DW, with MDGA showing the highest encapsulation efficiency. The effects of encapsulation on microbial inhibition, physicochemical properties, and sensory quality were investigated over 15 days of storage. Aerobic plate counts in the control increased from 3.04 to 8.26 log CFU/g, whereas encapsulated treatments showed significantly lower final counts (p < 0.05), reaching 7.89 log CFU/g (MD), 7.96 log CFU/g (MDGA), and 7.95 log CFU/g (GA). Similarly, encapsulated treatments reduced Escherichia coli counts during storage, with maltodextrin (MD) exhibiting the greatest inhibitory effect (6.23 × 10⁵ CFU/g) compared with the control (6.93 × 10⁵ CFU/g) on day 15. However, reductions in Staphylococcus aureus, E. coli, Candida albicans, and Bacillus cereus remained below 1 log CFU/g, indicating limited antimicrobial efficacy under the tested conditions. All encapsulated treatments slowed pH increases during storage (6.20–6.34) relative to the control (6.62) on day 15 and preserved aroma quality throughout the storage period. Overall, encapsulation improved the antimicrobial performance of V. sinaiticum extract compared with the free extract, particularly in MD-based systems; however, the antimicrobial effects in beef remained modest. These findings highlight both the potential and current limitations of encapsulated plant-derived antimicrobials for meat preservation and emphasize the need for optimized delivery systems to enhance efficacy in complex food matrices. Full article

Background: Citrulline malate (CM) is commonly used as an ergogenic supplement, but its effects on exercise performance and perceived exertion remain uncertain. This systematic review and meta-analysis evaluated the effects of CM supplementation, with attention paid to differences between acute and chronic protocols. Methods: Six databases were searched from inception to August 2025. Randomized controlled trials examining CM supplementation on exercise performance and/or perceived exertion were included. Hedges’ g was synthesized using three-level random-effects models to account for dependent effect sizes. Subgroup and moderator analyses explored supplementation protocol, exercise modality, sex, training status, dosage, and ingestion timing. Risk of bias, small-study effects, sensitivity analyses, and GRADE certainty were assessed. Results: Thirty randomized controlled trials contributed 138 effect sizes from 644 participants. CM supplementation was associated with a small improvement in overall exercise performance (g = 0.16, p = 0.01); however, prediction intervals were wide and statistical power was limited. The pooled effect on perceived exertion was not statistically significant. Current evidence appeared more stable for acute than chronic supplementation, although the protocol subgroup difference remained uncertain. Among acute studies, exploratory subgroup analyses suggested possible benefits for aerobic endurance and short anaerobic tasks, but these findings were not robust across sensitivity analyses. No significant between-subgroup differences were found for sex, training status, dosage, or ingestion timing. GRADE certainty ranged from low to very low. Conclusions: CM supplementation may be associated with small, context-dependent improvements in exercise performance, but current evidence remains limited and uncertain. Reliable dosing, timing, and target populations have not been established. Larger trials with verified supplement composition and standardized protocols are needed. Full article