Search Results (15,382)

Background/Objectives: Recent research highlights Vitis vinifera seeds as a rich source of bioactive proanthocyanidins (PACs) with antioxidant and immunomodulatory effects. Poorly absorbed PACs are metabolized by gut microbiota into active phenolic metabolites. This pilot study in ulcerative colitis patients assessed grape seed extract effects on microbiota, zonulin-related permeability, and quality of life. Methods: This prospective pilot study, conducted at the University Hospital of Padua, evaluated the effects of an eight-week treatment with proanthocyanidins (ECOVITIS^®) on gut microbiota, intestinal permeability (zonulin), and well-being in patients with ulcerative colitis in remission (IBDQ). Fecal and serum samples were collected at T0 and T1. Microbiota analysis was performed through 16S rRNA gene sequencing (QIIME2), zonulin was quantified using an ELISA kit for pre-haptoglobin gene2 (pre-HP2), and HP1/HP2 genotyping was conducted by quantitative PCR. Statistical analyses (Wilcoxon, ALDEx2, PERMANOVA) assessed microbial diversity and taxonomic changes between pre- and post-treatment samples. Results: Twenty-five ulcerative colitis patients completed the study. IBDQ scores significantly improved after treatment (mean Δ = +11.2, p < 0.001), especially in the 11 best IBDQ responders (Δ = +24.2, p < 0.001). Microbiota analysis showed increased Lachnospiraceae and Sutterellaceae in responders, while overall diversity remained unchanged. Zonulin levels were unaffected. Conclusions: PAC treatment improved quality of life in ulcerative colitis patients, as shown by increased IBDQ scores. Serum zonulin levels remained unchanged. Microbiota analysis revealed enrichment of Lachnospiraceae and Sutterellaceae families, suggesting beneficial modulation. Limitations include lack of metabolic assessment and a control group, and caution is needed in interpreting zonulin measurements. Full article

In achieving sustainable development goals, soils play a key role in environmental protection, natural resources, and food security. Peatlands are particularly important here, as they function at the interface between terrestrial and aquatic ecosystems and store large amounts of organic matter. However, organic soils are highly susceptible to transformation and degradation; therefore, their degradation caused by, among others, drainage properties is a high risk to both the environment and agriculture—it disrupts the ecosystems, causes greenhouse gas emissions, and eutrophicates the hydrosphere. Soil degradation in drained peatlands is associated with the transformation of soil organic matter (SOM), which in organic soils is the dominant component of the solid phase of the soil. The aim of our study was to assess the properties and degree of organic matter transformation in drained temperate peatland soils, with particular emphasis on sequential fractionation of SOM and humic acid properties. Due to the fact that in Poland, as many as 90% of non-forest peat bogs have been drained, we compare the mursh horizons that formed after peat bog drainage with the peat horizons that constitute the parent rock (where anaerobiosis occurs and morphological changes in the soil material are absent due to peat bog drainage). Studies were conducted on 11 soil profiles located in central-eastern Poland. Basic physicochemical soil properties were determined: pH, bulk density, contents of ash, SOM, total carbon (TC), and total nitrogen (TN). Sequential carbon fractionation was used to qualitatively analyze organic matter, which allowed for the identification of labile fractions, lipid fractions, humic substances (fulvic and humic acids), and residual fractions. Humic acids (HAs) were extracted using the Schnitzer method and analyzed for their elemental composition and spectrometric parameters in the VIS range. It was demonstrated that SOM transformation in drained temperate peatland soils was correlated with comprehensive changes in the soil’s physical and chemical properties. Compared to peat horizons, topsoil horizons were characterized by higher ash content and density, lower SOM content, and a lower TC/TN ratio. Qualitative SOM transformation during aerobic SOM transformation after draining the studied peatlands consisted of an increase in the amount of labile fractions and humic substances and a decrease in the lipid and residual fractions. The research results have shown that the HAs properties depended on the depth. HAs from topsoil horizons, compared to peat horizons, were characterized by a lower “degree of maturity,” as reflected by the values of atomic ratios (H/C, O/C) and absorbance coefficients (A_4/6 and ΔlogK). It was found that the share of the distinguished SOM fractions and HAs properties were closely correlated with the physical and chemical properties of the soils. The study demonstrated the usefulness of the sequential carbon fractionation method for assessing the effects of dewatered peat transformation. The obtained results could contribute to the development of good practices ensuring high quality of organic matter and stability of ecosystems, as well as to the development of methods for limiting the mineralization of organic matter (SOM), greenhouse gas emissions, and the loss of organic soils in agricultural areas. Full article

Forest canopies effectively remove airborne particles, reducing the frequency of atmospheric haze and improving air quality as well as playing a crucial role in maintaining human health. In this study, we examined the retention of particulate matter by Picea crassifolia Kom. (P. crassifolia) needles using an aerosol regenerator in two typical catchments, while the concentrations of dissolved trace elements (Na, Zn, Pb, and Cd) were determined only in the Tianlaochi catchment. The results showed that the retention of airborne particles was lower in the Tianlaochi catchment (e.g., total suspended particles (TSP): 0.0049 μg cm⁻² in summer) than in the Sancha catchment (e.g., TSP: 0.0145 μg cm⁻²) in summer and autumn, while the opposite trend was found in winter and spring, with Tianlaochi catchment reaching higher TSP levels (0.0230 μg cm⁻² in spring) compared to Sancha catchment (0.0205 μg cm⁻²). The big tree exhibited the highest particulate retention, with a maximum flux of 84.870 μg cm⁻², indicating it was the most effective at particle trapping. The highest Na, Zn, Cd, and Pb values absorbed by the needle samples were 1.794 mg L⁻¹, 11.345 μg L⁻¹, 0.081 μg L⁻¹, and 4.316 μg L⁻¹, respectively. P. crassifolia needles absorbed more Na, Zn, and Cd in July and August than in June. The absorption capacity of the needles decreased in the order Na > Zn > Pb > Cd. P. crassifolia forest can effectively reduce airborne particulate matter. Our study provides a theoretical foundation to examine the role of forest ecosystems in the retention of atmospheric particulate matter in the Qilian Mountains region. Full article

In this study, we report a reproducible in situ photochemical method for the simultaneous synthesis of metallic and hybrid metal/metal oxide nanoparticles (NPs) within a UV–curable polymer matrix. A series of epoxy diacrylate-based formulations (BEA) was prepared, consisting of Epoxy diacrylate, Di(Ethylene glycol)ethyl ether acrylate (DEGEEA), and Phenylbis (2,4,6-trimethylbenzoyl) phosphine oxide (BAPO), which served as a Type I photoinitiator. These formulations were designed to enable the simultaneous photopolymerization and photoreduction of metal precursors at various Ag⁺/Co²⁺ ratios, resulting in nanocomposites containing in situ-formed Ag NPs, cobalt oxide NPs, and hybrid Ag–Co₃O₄ nanostructures. The photochemical, magnetic, and dielectric properties of the resulting nanocomposites were evaluated in comparison with those of the pure polymer using UV–Vis and Fourier Transform Infrared Spectroscopy (FT-IR), Photo-Differential Scanning Calorimetry (Photo-DSC), Thermogravimetric Analysis (TGA), Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), Impedance Analysis, and Vibrating Sample Magnetometry (VSM). Photo-DSC studies revealed that the highest conversion values were obtained for the BEA-Ag₁Co₁, BEA-Co, and BEA-Ag₁Co₂ samples, demonstrating that the presence of Co₃O₄ NPs enhances polymerization efficiency because of cobalt species participating in redox-assisted radical generation under UV irradiation, increasing the number of initiating radicals and leading to faster curing and higher final conversion. On the other hand, the Ag NPs, due to the SPR band formation at around 400 nm, compete with photoinitiator absorbance and result in a gradual decrease in conversion values. Crystal structures of the NPs were confirmed by XRD analyses. The dielectric and magnetic characteristics of the nanocomposites suggest potential applicability in energy-storage systems, electromagnetic interference mitigation, radar-absorbing materials, and related multifunctional electronic applications. Full article

The territorial development of the city of Brno during the 19th–21st centuries meant not only the growth of built-up areas (residential, industrial, commercial), but also the absorbing of segments of the ancient rural agricultural landscape. Within the current borders of the city of Brno, a number of green areas have been preserved, which have spontaneously developed from the original agricultural landscape, without being the result of urban planning. In half of the cases (17 out of a total of 34), they have still preserved the traditional small-scale division of land. Among the 10 medium-sized Moravian cities (between 30,000 and 400,000 inhabitants) in the historical region of Moravia in the east of the Czech Republic, the presence of 34 remnants of the ancient rural landscape in the city of Brno is quite exceptional (in Ostrava only 1; in other cities 0). The subject of the research is the inventory of such segments within the city borders and an attempt to explain their location in the city, state, focusing on the role of natural factors, land ownership and personal and recreational interests of residents. Segments of the ancient rural cultural landscape were identified by comparing the current landscape on aerial photographs with the landscape image on cadastral maps from the 1820s–1830s. Additional data on their natural and cultural properties were obtained through archival and field research. The segments were classified according to their degree of preservation and forms of threat. The results show that the remains of the ancient rural cultural landscape in the city of Brno have generally been preserved in locations that, due to the slope of the slopes, unsuitable building subsoil and poor soil, but locally on warm southern slopes, were not suitable for construction for the time being. Urban gardening contributes to their preservation and these areas are part of the city’s greenery. However, urban gardening also contributes to the destruction of these remnants. In 17 cases, the land was completely re-divided, built up with recreational facilities and overgrown with trees due to poor care. Another 17 locations are threatened by this process due to ignorance of their historical value, although this is essentially a positive development in terms of benefits for the city’s residents—land users. Although the Master Plan of the city of Brno foresees the existence of garden colonies in the future, it does not address the importance of the best-preserved segments as historical heritage. Community agriculture can play a positive role in maintaining segments of rural heritage within the city. Full article

Recurrent brain tumors—including high-grade gliomas, brain metastases, and aggressive meningiomas—continue to carry a poor prognosis, with high mortality despite therapeutic advances. The aim of this narrative review is to summarize and critically discuss the current evidence on the role of intra-arterial radioligand therapy (RLT) in the treatment of recurrent brain tumors. RLT, a targeted form of radionuclide therapy, has gained increasing attention for its potential theranostic applications in neuro-oncology. A literature search was conducted using PubMed and Scopus, including clinical studies evaluating intra-arterial radioligand delivery in central nervous system tumors. Recent research has explored intra-arterial administration of radioligands targeting somatostatin receptors and prostate-specific membrane antigen (PSMA). Somatostatin receptors are overexpressed in meningiomas, while PSMA is highly expressed in the neovasculature of glioblastomas and brain metastases; both targets can be addressed using lutetium-177 (¹⁷⁷Lu)- or actinium-225 (²²⁵Ac)-labeled radiopharmaceuticals, traditionally delivered intravenously. Available evidence indicates that the intra-arterial route achieves markedly higher radionuclide uptake on ⁶⁸Ga-PSMA-11 and ⁶⁸Ga-DOTATOC PET, as well as increased absorbed doses in dosimetric models. Dosimetric analyses consistently show greater tracer accumulation compared with intravenous administration, without evidence of significant peri-procedural toxicity. Uptake in healthy brain tissue is minimal, and no relevant differences have been reported in liver or salivary gland accumulation between intra-arterial and intravenous RLT. Although based on heterogeneous and limited data, intra-arterial RLT appears to be a promising therapeutic strategy for recurrent brain tumors. Future research should focus on improving radioligand delivery beyond the blood–brain barrier and enhancing effective tumor targeting. Full article

Additive manufacturing has been adopted in several industries including the medical field to develop new personalised medical implants including tissue engineering scaffolds. Custom patient-specific scaffolds can be additively manufactured to speed up the wound healing process. The aim of this study was to design, fabricate, and evaluate a range of materials and scaffold architectures for 3D-printed wound dressings intended for soft tissue applications, such as skin repair. Multiple biocompatible polymers, including polylactic acid (PLA), polyvinyl alcohol (PVA), butenediol vinyl alcohol copolymer (BVOH), and polycaprolactone (PCL), were fabricated using a material extrusion additive manufacturing technique. Eight scaffolds, five with circular designs (knee meniscus angled (KMA), knee meniscus stacked (KMS), circle dense centre (CDC), circle dense edge (CDE), and circle no gradient (CNG)), and three square scaffolds (square dense centre (SDC), square dense edge (SDE), and square no gradient (SNG), with varying pore widths and gradient distributions) were designed using an open-source custom toolpath generator to enable precise control over scaffold architecture. An in vitro degradation study in phosphate-buffered saline demonstrated that PLA exhibited the greatest material stability, indicating minimal degradation under the tested conditions. In comparison, PVA showed improved performance relative to BVOH, as it was capable of absorbing a greater volume of exudate fluid and remained structurally intact for a longer duration, requiring up to 60 min to fully dissolve. Tensile testing of PLA scaffolds further revealed that designs with increased porosity towards the centre exhibited superior mechanical performance. The strongest scaffold design exhibited a Young’s modulus of 1060.67 ± 16.22 MPa and withstood a maximum tensile stress of 21.89 ± 0.81 MPa before fracture, while maintaining a porosity of approximately 52.37%. This demonstrates a favourable balance between mechanical strength and porosity that mimics key properties of engineered tissues such as the meniscus. Overall, these findings highlight the potential of 3D-printed, patient-specific scaffolds to enhance the effectiveness and customisation of tissue engineering treatments, such as meniscus repair, offering a promising approach for next-generation regenerative applications. Full article

Radiative cooling (RC) offers a passive pathway to reduce surface and system temperatures by emitting thermal radiation through the atmospheric window, yet its daytime effectiveness is often constrained by geometry, angular solar exposure, and practical integration limits. This work experimentally investigates the use of passive non-imaging optics, specifically compound parabolic concentrators (CPCs), as enhancers of RC performance under realistic conditions. A three-tier experimental methodology is followed. First, controlled indoor screening using an infrared lamp quantifies the intrinsic heat gain suppression of a commercial RC film, showing a temperature reduction of nearly 88 °C relative to a black-painted reference. Second, outdoor rooftop experiments on aluminum plates assess partial RC coverage, with and without CPCs, under varying orientations and tilt angles, revealing peak daytime temperature reductions close to 8 °C when CPCs are integrated. Third, system-level validation is conducted using a modified GUNT ET-202 solar thermal unit to evaluate the transfer of RC effects to a water circuit absorber. While RC strips alone produce modest reductions in water temperature, the addition of CPC optics amplifies the effect by factors of approximately three for ambient water and nine for water at 70 °C. Across all configurations, statistical analysis confirms stable, repeatable measurements. These results demonstrate that coupling commercially available RC materials with non-imaging optics provides consistent and measurable performance gains, supporting CPC-assisted RC as a scalable and retrofit-friendly strategy for urban and building energy applications while calling for longer-term experiments, durability assessments, and techno-economic analysis before deriving definitive deployment guidelines. Full article

We employ the Dynamical Projective Operatorial Approach (DPOA) to investigate the ultrafast optical excitations of germanium under intense, ultrashort pump pulses. The method has very low resource demand relative to many other available approaches and enables detailed calculation of the residual electron and hole populations induced by the pump pulse. It provides direct access to the energy distribution of excited carriers and to the total energy transferred to the system. By decomposing the response into contributions from different multi-photon resonant processes, we systematically study the dependence of excited-carrier density and absorbed energy on key pump-pulse parameters: duration, amplitude, and photon energy. Our results reveal a complex interplay between these parameters, governed by resonant Rabi-like dynamics and competition between different multi-photon absorption channels. For the studied germanium setup, we find that two-photon processes are generally dominant, while one- and three-photon channels become significant under specific conditions of pump-pulse frequency, duration, and intensity. This comprehensive analysis offers practical insights for optimizing ultrafast optical control in semiconductors by targeting specific multi-photon pathways. Full article

Actinium-225 (²²⁵Ac) has emerged as a pivotal alpha-emitter in modern radiopharmaceutical therapy, offering potent cytotoxicity with the potential for precise tumour targeting. Accurate, patient-specific image-based dosimetry for ²²⁵Ac is essential to optimize therapeutic efficacy while minimizing radiation-induced toxicity. Establishing a robust dosimetry workflow is particularly challenging due to the complex decay chain, low administered activity, limited count statistics, and the indirect measurement of daughter gamma emissions. Clinical single-photon emission computed tomography/computed tomography protocols with harmonized acquisition parameters, combined with robust volume-of-interest segmentation, artificial intelligence (AI)-driven image processing, and voxel-level analysis, enable reliable time-activity curve generation and absorbed-dose calculation, while reduced mixed-model approaches improve workflow efficiency, reproducibility, and patient-centred implementation. Cadmium zinc telluride-based gamma cameras further enhance quantitative accuracy, enabling rapid whole-body imaging and precise activity measurement, supporting patient-friendly dosimetry. Complementing these advances, the cerium-134/lanthanum-134 positron emission tomography in vivo generator provides a unique theranostic platform to noninvasively monitor ²²⁵Ac progeny redistribution, evaluate alpha-decay recoil, and study tracer internalization, particularly for internalizing vectors. Together, these technological and methodological innovations establish a mechanistically informed framework for individualized ²²⁵Ac dosimetry in targeted alpha therapy, supporting optimized treatment planning and precise response assessment. Continued standardization and validation of imaging, reconstruction, and dosimetry workflows will be critical to translate these approaches into reproducible, patient-specific clinical care. Full article

Prevalence of LPS in Gram-negative bacterial udder infections determines mastitis severity and disease prognosis. This pilot study explores the notion that milk-soluble (s) LPS/IgG complex levels in dairy cows link mastitis severity to intramammary Gram-negative infections during early lactation. Milk, within a single herd, was analysed from (i) 34 early lactating cows with acute mastitis and (ii) milk selected from peak lactation cows displaying either healthy (SCC < 100 × 10³ cells/mL, n = 146) or subclinical mastitis (SCC > 150 × 10³ cells/mL, n = 135) characteristics. Milk was assessed for (i) sLPS/IgG using an “in-house” ELISA, (ii) udder inflammation using LDH activity, and (iii) bacterial presence applying on-farm and standard microbiological laboratory techniques. Mean milk sLPS/IgG absorbances in acute mastitis cows were higher than those detected in healthy and subclinical mastitis cows, with mean differences of 0.35 (95% CI, 0.28 to 0.42) and 0.36 (95% CI, 0.28 to 0.44), respectively. On day 1 of acute mastitis, sLPS/IgG levels in milk containing only Gram-positive bacteria ranged from OD 0.04 to 0.14 (median = 0.1). In contrast, sLPS/IgG levels ranging from OD 0.27 to 1.42 (median = 0.58) and from 0.02 to 1.67 (median = 0.21) were detected in milk containing only Gram-negative bacteria or both Gram-negative and Gram-positive bacteria (i.e., polymicrobial), respectively. Furthermore, differential milk sLPS/IgG absorbance profiles (observed during the testing period days 1–3) were observed in cows with acute mastitis caused by Gram-positive, Gram-negative or polymicrobial infections. Our preliminary findings support the notion that milk sLPS/IgG complexes provide a link between mastitis severity and intramammary Gram-negative infections in dairy cows during early lactation. Full article

Background/Objectives: Essential amino acids’ (EAAs) biological effects depend on both gastrointestinal stability and intestinal bioavailability. A commercially available EAA blend has previously shown to be highly bioaccessible and able to inhibit the DPP-IV enzyme both directly and at a cellular level following simulated digestion in vitro. In light with this consideration, the present study aimed to evaluate the intestinal in vitro bioavailability of GAF subjected to INFOGEST digestion (iGAF) and to investigate the metabolic effects of its bioavailable fraction on muscle cells using an integrated Caco-2/C2C12 co-culture model. Methods: Differentiated Caco-2 cell lines were treated with iGAF, and amino acid transport was quantified by ion-exchange chromatography. The basolateral fraction containing bioavailable EAAs was used to treat differentiated C2C12 myotubes for 24 h. Western blot analyses were performed to assess the activation of anabolic and metabolic pathways, including mTOR, Akt, GSK3, AMPK and GLUT-4. Results: More than 50% of each EAA present in iGAF crossed the Caco-2 monolayer, with BCAAs and phenylalanine particularly enriched in the basolateral fraction. Exposure of C2C12 myotubes to the bioavailable iGAF stimulated mTORC1 activation and increased the phosphorylation of Akt and GSK3, indicating an enhanced anabolic response. At a cellular level, iGAF also elevated the p-AMPK/AMPK ratio, suggesting activation of energy-sensing pathways. Moreover, GLUT4 protein levels and glucose uptake were significantly increased. Conclusions: The study focuses exclusively on a cellular model, and results suggested that iGAF is highly bioavailable in vitro and that its absorbed fraction activates key anabolic and metabolic pathways of skeletal muscle cells, enhancing both protein synthesis signaling and glucose utilization in vitro. Full article

Achieving dynamic stability in bipedal locomotion against sustained external disturbances remains a significant challenge in humanoid robotics. Traditional methods, such as zero moment point (ZMP) preview control, often lack the reactive compliance and predictive planning necessary for robust performance on uneven terrain or under continuous force. This paper proposes a novel control framework that synergistically integrates a resistance torso compliance controller with a nonlinear model predictive control (NMPC)-based walking pattern generator. The compliance controller actively modulates the torso’s dynamics via impedance control, creating a virtual mass–spring–damper system that absorbs impacts and generates counterforces to resist sustained pushes. Concurrently, the NMPC module reformulates gait generation as a real-time optimization problem, simultaneously determining optimal footstep positions and orientations while respecting nonlinear constraints derived from centroidal momentum dynamics. Simulation results demonstrate that this integrated approach reduces the maximum ZMP error by 34.1% and the RMS ZMP error by 37.3% compared to traditional ZMP preview control, with a 38.9% improvement in settling time after a disturbance. This work establishes that the tight coupling of reactive impedance control and predictive optimization provides a foundational framework for enhancing the robustness and adaptability of bipedal locomotion. Full article

This study utilizes COMSOL Multiphysics (version 6.0) to design a planar ultra-broadband optical absorber with a multilayer configuration. The proposed structure consists of seven stacked layers arranged from bottom to top: W (h1, acting as a reflective substrate and transmission blocker), WSe₂ (h2), SiO₂ (h3), Ni (h4), SiO₂ (h5), Mo (h6), and SiO₂ (h7). One key finding of this study is that, when all other layer thicknesses are fixed, variations in the Mo layer thickness systematically induce a redshift in both the short- and long-wavelength cutoff edges. Notably, the long-wavelength cutoff exhibits a larger shift than the short-wavelength edge, resulting in an increased absorption bandwidth where absorptivity remains above 0.900. The second contribution is the demonstration that this planar structure can be readily engineered to achieve ultra-broadband absorption, spanning from the near-ultraviolet and visible region (360 nm) to the mid-infrared (6300 nm). An important characteristic of the proposed design is that the thickness of the h7 SiO₂ layer influences the cutoff wavelength at the short-wavelength edge, while the thickness of the h6 Mo layer governs the cutoff position at the long-wavelength edge. This dual modulation capability allows the proposed optical absorber to flexibly tune both the spectral range and the bandwidth in which absorptivity exceeds 0.900, thereby enabling the realization of a wavelength- and bandwidth-tunable optical absorber. Full article

This study develops a hyperbolic tangent-based model for the hysteretic behavior of automotive grade electrohydraulic semi-active dampers. Model parameters were identified from experimental force–velocity data gathered under sinusoidal excitations across 1–6 Hz and 0.38–1.6 A. The calibrated model was integrated into an IPG CarMaker 13.0/Simulink 2022b co-simulation to assess performance under ISO-compliant road profiles and realistic driving scenarios. Comparative analysis with conventional nonlinear damper models was conducted, focusing on ride comfort metrics such as vertical acceleration, pitch rate, and roll rate. The results demonstrate that the proposed model provides improved fidelity in replicating real damper behavior and enables more realistic assessment of semi-active suspension performance in virtual vehicle development platforms by providing reduced vertical acceleration errors by >5 dB (2–6 Hz) compared to nonlinear models. Full article