Search Results (22)

This study aims to characterize tissue-specific expression patterns in Hanwoo steers by identifying co-expression modules, functional pathways, and hub genes related to fat and muscle traits using Weighted Gene Co-expression Network analysis (WGCNA). RNA-Seq data were generated from three muscle tissues (longissimus muscle, tenderloin, and rump) and two fat tissues (back fat and abdominal fat) collected from six 30-month-old Hanwoo steers. Quality control of raw sequencing reads was performed using FastQC, and trimmed reads were aligned to the bovine reference genome (ARS-UCD1.3) using HISAT2. We also identified a gene co-expression network via WGCNA using normalized gene expression values. Modules were defined based on topological overlap and correlated with tissue-specific expression patterns. Modules with a significant association (p < 0.05) were used for functional enrichment based on Gene Ontology (GO) and KEGG pathways, as well as Protein–Protein Interaction Network analysis. A total of seven co-expression modules were identified by WGCNA and labeled in distinct colors (yellow, blue, red, brown, turquoise, green, black). Among them, the yellow and blue modules were positively associated with back fat, while the turquoise and green modules showed a negative correlation with abdominal fat. Additionally, the turquoise or green module was positively correlated with longissimus and rump tissues, indicating distinct gene expression patterns between fat and muscle. This study identified key co-expression modules and hub genes associated with muscle and fat metabolism. Notably, ARPC5 (blue module) was involved in lipid metabolism and energy storage, whereas AGPAT5 (turquoise module) was linked to maintaining muscle cell structure and function. These findings reveal biological mechanisms for tissue-specific gene regulation, providing targets for enhancing meat quality in Hanwoo. Full article

A total of 700 broiler chicks (350 Indian River and 350 Ross 308) were obtained from a local hatchery. Birds were randomly allocated in a completely randomized design by strain into two dietary groups: a control group (corn–soybean meal) and a treatment group in which 7.5% of soybean meal (SBM) was replaced with fermented soybean meal (FSBM). Each group included 175 birds, distributed across seven replicates (pens) with 25 birds per pen. Birds were weighed at the start of the trial and at the end of each rearing phase (starter (1–14 days of age), and grower phases (15–35 days of age)). Mortality was monitored daily throughout the experiment. Weekly feed intake was recorded for each pen. On day 35, 14 birds were randomly selected from each treatment–strain combination for carcass and meat quality evaluation. Data were analyzed with linear models; strain, diet, and their interaction were the investigated effects. No strain–diet interaction was detected for growth performance, carcass and meat quality, and intestinal morphology traits (p > 0.05). Significant interaction effect on crude protein digestibility is found (p < 0.001). The FSBM improved crude protein digestibility for the Indian River broiler strain, while the opposite occurred in the Ross 308 strain. Fermented soybean meal supplementation did not significantly affect body weight gain, feed conversion ratio (FCR), or feed intake during the study period (p > 0.05). The Ross strain exhibited a higher dressing percentage (p = 0.04) and greater cooking loss (p = 0.01), whereas the Indian River strain showed higher abdominal fat percentage (p = 0.04) and shear force (p = 0.003). The dressing percentage, pH, color parameters, cooking loss, water-holding capacity, and breast fillet yield for FSBM and control groups did not differ (p > 0.05). Ileum villus length and width were significantly higher (p < 0.05) in the FSBM group compared to the control group. In conclusion, Fermented soybean meal can enhance intestinal morphology and crude protein digestibility in a strain-specific manner; however, its impact on growth and carcass attributes is limited at this inclusion level. Full article

This experiment evaluated the effects of including buriti oil (BO) in the diet of broilers on growth performance, nutrient digestibility, carcass yield, meat quality, blood parameters, intestinal morphometry, the proliferation of collagen fibers in the skin and collagen concentration in the breast muscle. A total of 180 one-day-old Ross male chicks were distributed in a completely randomized experimental design with three treatments (0%, 1% and 2% BO) and six replications of 10 birds each. Nutrient digestibility was evaluated from 8 to 21 days, while the performance, carcass yield, meat cut yields, abdominal fat, meat quality, blood parameters, intestinal morphometry and skin histology of the birds were evaluated after 21 days. The inclusion of BO significantly affected (p ≤ 0.05) performance, digestibility, meat color, lymphocyte count, duodenal morphometry and collagen concentration. In addition, it increased the metabolizable energy of the diet and reduced the birds’ performance, indicating that the nutraceutical effect of BO improved nutrient use. However, it caused an excess of energy that had to be metabolized by the birds, consequently affecting their performance. BO increased the pigmentation of the breast meat and reduced the lymphocyte count, probably due to its antimicrobial action, consequently decreasing the recruitment of defense cells. An increase in the height of the crypt of the duodenum was observed as the inclusion of BO increased, with the opposite being observed in the jejunum. The inclusion of BO increased the histological proliferation of collagen from minimal (0%) to medium (1%) and moderate (2%), as well as the collagen concentration (CC = 35.933 + 4.677BO; R² = 0.80). Thus, due to functional/nutraceutical effects, adding BO in the diet of broilers can be a promising alternative to antibiotic growth promoters to improve poultry production. Full article

In order to investigate the nutritional processing quality of sika deer (Cervus nippon) venison at different sites, the pH_{24 h}, tenderness, pressurized water loss rate, meat color, intramuscular fat, moisture, protein, amino acid, fatty acid and squalene contents of sika deer venison were determined in twelve sites: foreleg, hind leg, outer tenderloin, rump, neck meat, chest meat, deer flank, abdominal rib, high rib, tenderloin, anterior tendon and posterior tendon. The results showed that the pH_{24 h} of sika deer venison at different sites was 5.49~5.78; the tenderness of outer tenderloin (31.71 N) was the lowest, and the neck meat (68.53 N) was the highest; the squeezing moisture of tenderloin (28.12%) was the largest, and the foreleg (12.34%) was the smallest; the brightness of outer tenderloin L* (29.68) was the lowest, and the redness a* and yellowness b* of deer flank were the highest; the intramuscular fat and moisture were 0.66~4.97% and 71.00~73.78%, respectively; and the protein content of outer tenderloin (23.44%) and rump (24.02%) was high. The venison meat contained 17 kinds of amino acids, and the total amount was 63.87~79.33 g/100 g. It was rich in essential amino acids, mainly lysine and leucine, accounting for 64.29~65.39% of non-essential amino acids, which was close to the ideal protein composition. Palmitoleic acid and oleic acid were the main monounsaturated fatty acids in venison, and the contents of abdominal ribs were the highest, 16,875.33 mg/kg and 31,772.73 mg/kg, respectively. The contents of essential fatty acids were also the highest in abdominal ribs (11,225.37 mg/kg); forelegs, hind legs, outer tenderloins, rumps, neck meat, chest meat, high rib, tenderloins, anterior tendons and posterior tendons were all good sources of polyunsaturated fatty acids. Squalene content was highest in the abdominal rib (100.85 mg/kg). The nutritional processing quality of sika deer venison in different muscles is significantly different, and this study can provide a data basis for the evaluation and processing of sika deer venison quality. Full article

This study aimed to compare the effects of incorporating fermented feed into daily diets on the slaughter performance, meat quality, and flavor compounds of 120 domestic chickens over a 140-day period. A total of five groups (n = 24), including the control group (CK) of the Guangxi Partridge chickens received a standard base diet. The other four groups were provided with pellets that had been added with 10% fermented banana peel (Pe-10), 20% fermented banana peel (Pe-20), 10% fermented banana pulp residue (Pu-10), and 20% fermented banana pulp residue (Pu-20). The flavor compounds in the meat samples of the chickens in these groups were determined using the gas chromatographic method. The results demonstrated that the chickens in the Pe-10, Pe-20, Pu-10, and Pu-20 groups exhibited pectoral muscle percentages, thigh muscle percentages, and total fatty acid content of chest meat that were higher than those observed in the CK group. The moisture content, meat color, carcass weight, total net weight, and abdominal fat percentage of the meat samples in these experimental groups exhibited no notable differences. The flavor compounds in the meat samples of the chickens fed with the two concentrations of fermented banana peel and banana residue were found to be significantly different from those in the control group, with p-values less than 0.05. As the quantity of fermented banana peel incorporated into the daily ration was increased from 10% to 20%, a notable alteration in the flavor compounds present in the chicken samples was observed. The chickens that were provided with fermented banana peels and pulps in their diets exhibited superior slaughter performance and meat quality, particularly in the case of the Pu-10 group, in comparison to the control chickens. Full article

Unlike other chicken breeds, Xichuan Black Bone (XBB) chickens are an established breed in China with excellent production performance and unique characteristics, including black meat, beaks, skin, bones, and legs, and they produce blue-shelled eggs. The Tengchong Snow (TS) chicken breed has relatively lower growth performance than commercial breeds but is considered one of the main genetic treasures of black meat in China. To improve the production and meat quality traits of the TS chickens by hybridization, the current study aimed to investigate the growth performance, carcass indices, meat quality physical properties, and muscle fiber traits of fiber traits of F1 crosses of TS with XBB chickens. Three groups of crossbreeding combinations were produced: (1) XT group (XBB × TS ), (2) TX group (TS × XBB ), and (3) TT group (TS × TS ), with the TT group used as a control. A total of 725 healthy chicks (XT group: 247, TX group: 180, TT group: 298) were reared up to 20 weeks of age to estimate the growth performance and associated meat parameters. The results showed that the XT and TX groups had higher body weight and body size compared with the TT group (p < 0.05). Similarly, breast width, breast length, width of body, and carcass weights were also greater (p < 0.05) in the XT and TX groups compared with the TT group. Meat physical properties, including color, water-holding capacity, and tenderness, were improved (p < 0.05) for the XT and TX group compared to the TT group. The XT group had the better color of the leg muscles with the unique orientation of muscle fibers. Based on the results, the XT group is more in line with the future breeding direction as they have greater body weight, larger size, and lower abdominal fat. This study is a baseline technical reference for the protection, evaluation, and utilization of germplasm resources of Tengchong Snow chicken for screening the best matching lines and combinations with local chickens. Full article

Abdominal fat, which in the past was often regarded as waste and discarded, has in recent years been used as a fat source to produce meat by-products. Yellow abdominal fat has higher economic value. Therefore, improving the color of abdominal fat plays an important role in improving the appearance of meat products. This study aimed to identify the contributors and the regulatory network involved in the formation of yellow and white color in abdominal fat. We found that four xanthophyll compounds were significantly different in yellow and white abdominal fat chicken, including zeaxanthin, lutein, canthaxanthin, and β-cryptoxanthin. There were 551 different and 8 common metabolites significantly correlated with these 4 xanthophyll compounds. Similarly, a total of 54 common genes were identified in 4 common related pathways (Complement and coagulation cascades, Metabolic pathways, PPAR signaling pathway, Carbon metabolism) of the 8 common metabolites. The high expression of HAAO in the yellow abdominal fat group leads to the degradation of tryptophan and its intermediate 5-hydroxyindole, and subsequently to the formation of the four xanthophyll compounds. This process is also regulated by tyrosine, kynurenine 3-monooxygenase (KMO), homogentisate 1, 2-dioxygenase (HGD), etc. Together, these findings show the effect of tryptophan on abdominal fat color, as well as a negative regulatory effect of HAAO and 5-hydroxyindole on the production of xanthophyll compounds involved in abdominal fat coloration. Full article

This study evaluated the effect of free and nanoencapsulated rosemary essential oil (REO) as an antibiotic alternative in broiler diets on growth performance, nutrient digestibility, carcass traits, meat quality and gene expression. Four hundred twenty day-old commercial broiler chicks (VENCOBB) were randomly allocated to seven dietary treatments, each having four replicates of fifteen chicks. The dietary treatments comprised control (CON) fed a basal diet only, AB (basal diet + 10 mg enramycin/kg), CS (basal diet + 150 mg chitosan nanoparticles/kg), REO_F100 and REO_F200 (basal diet + 100 mg and 200 mg free REO/kg, respectively), and REO_N100 and REO_N200 (basal diet + 100 mg and 200 mg nanoencapsulated REO/kg, respectively). Overall (7–42 d), REO_N200 showed the highest (p < 0.001) body weight gain (1899 g/bird) and CON had the lowest gain (1742 g/bird), while the CS, REO_F100 and REO_F200 groups had a similar gain, but lower than that of the AB and REO_N100 groups. Feed intake was not affected by dietary treatments. Overall, the feed efficiency increased (p = 0.001) by 8.47% in the REO_N200 group and 6.21% in the AB and REO_N100 groups compared with the CON. Supplementation of REO improved (p < 0.05) dry matter and crude protein digestibility, with the highest values in REO_N100 and REO_N200. Ether extract, crude fiber, calcium and phosphorus digestibility values showed no difference among the groups. The dressing, breast, thigh % increased (p < 0.05) and abdominal fat % decreased (p < 0.001) more in the REO_N200 group than with other treatments and CON. In breast meat quality, water holding capacity and extract reserve volume increased (p < 0.05) while drip loss and cholesterol content decreased (p < 0.05) in REO_N100 and REO_N200. No change was observed in the breast meat color among dietary treatments and CON. The REO_N100 and REO_N200 groups had reduced (p < 0.05) meat lipid peroxidation as depicted by the decreased levels of TBARS, free fatty acids and peroxide value compared to other treatments and CON. The expression of the Mucin 2, PepT1 and IL-10 genes was upregulated (p < 0.001) and TNF-α downregulated (p < 0.001) by dietary addition of REO particularly in the nanoencapsulated form compared with the CON. In conclusion, nanoencapsulated REO, especially at 200 mg/kg diet, showed promising results as an antibiotic alternative in improving the performance, nutrient digestibility, carcass traits, meat quality and upregulation of growth and anti-inflammatory genes. Full article

This study aimed to assess the effects of different dietary vitamin D₃ (VD₃) levels on growth and carcass performance, tibia traits, meat quality, and intestinal morphology of yellow-feathered broilers. One-day-old broilers (n = 1440) were assigned into four treatment groups with six replicates per group, and each replicate contained 60 chicks. Dietary VD₃ significantly improved the growth performance and carcass traits of broilers, and only low-dose VD₃ supplementation decreased the abdominal fat percentage. High-dose VD₃ supplementation improved intestinal morphology in the finisher stage, while the b* value of breast muscle meat color decreased markedly under VD₃ supplementation (p < 0.05). Serum Ca and P levels and the tibia composition correlated positively with dietary VD₃ supplementation at the early growth stage. The weight, length, and ash contents of the tibia increased linearly with increasing dietary VD₃, with maximum values achieved in the high-dose group at all three stages. Intestinal 16S rRNA sequencing and liver transcriptome analysis showed that dietary VD₃ might represent an effective treatment in poultry production by regulating lipid and immune-related metabolism in the gut–liver axis, which promotes the metabolism through the absorption of calcium and phosphorus in the intestine and improves their protective humoral immunity and reduces infection mortality. Dietary VD₃ positively affected the growth—immunity and bone development of broilers during the early stage, suggesting strategies to optimize poultry feeding. Full article

The aim of this research is to compare the carcass composition and meat quality characteristics of spent Cobb 500 and Ross 308 broiler breeders. A total of 28 carcasses were evaluated—7 female and 7 male carcasses from each genotype. Dissection was performed, and the percentages of neck, wings, skin with subcutaneous fat, abdominal fat, residual components, breast and leg muscles were calculated relative to the eviscerated carcass weight. The breast and leg muscles were evaluated for their chemical composition, color attributes (Lab), acidity (pH₂₄), and electrical conductivity (EC₂₄). Analysis of the structure and texture of the pectoralis major muscle was performed. The genotype of the birds had an impact on the eviscerated carcass weight, percentage of skin with subcutaneous fat, leg muscles, wings, and neck. Broiler breeder genotypes differed in terms of the chemical composition of the breast and leg muscles, except for the water content in the breast muscle and the collagen content in both the breast and leg muscles. The breast muscles of Cobb 500 exhibited lower cooking loss, pH₂₄, redness, and yellowness, while the leg muscles of Ross 308 had lower EC₂₄ but higher cooking loss and lightness values. The pectoralis major muscle of Cobb 500 was firmer and more tender, with a smaller cross-sectional area of the muscle fiber and a smaller vertical (V) diameter of the muscle fiber. Males were characterized by a greater carcass weight and a higher percentage of leg muscles, neck, and carcass remains. On the other hand, females had a higher percentage of breast muscles, skin with subcutaneous fat, and abdominal fat. The sex of the birds affected the chemical composition of the breast and leg muscles, with the exception of the water content in the breast muscles and collagen content in the breast and leg muscles. The breast muscles of females were characterized by higher values of yellowness, although they also exhibited lower pH and cooking loss. In terms of texture analysis of the pectoralis major muscle, the meat of females was characterized by higher tenderness and firmness. However, the analysis of the structure showed that males had a thicker perimysium and endomysium. Regardless of broiler origin and sex, significant differences were found between the breast and leg muscles in terms of the assessed physicochemical features (pH₂₄, EC₂₄, cooking loss), color attributes (Lab) and chemical composition (protein, intramuscular fat, and collagen contents). Genotype and sex interactions were significant for the chemical composition of the breast muscles (protein, fat, collagen) and leg muscles (fat), as well as for the yellow color saturation of the breast muscles and springiness of the pectoralis major muscle. The study produced results that showed the meat and carcasses of spent hens and roosters to be suitable for processing due to their favorable chemical composition, high nutritional value and good technological properties, as assessed based on the results of meat texture and structure. Full article

This study aimed to identify major dietary patterns associated with abdominal obesity in middle-aged and older Korean adults. Data from the Korean Genome and Epidemiology Study were used. A total of 48,037 Korean adults aged ≥40 years without abdominal obesity at baseline were followed-up. Dietary assessment was conducted using a validated 106-item food-frequency questionnaire, and dietary patterns were identified using factor analysis. Abdominal obesity was defined as a waist circumference of ≥90 cm for men and ≥85 cm for women, according to the Korean Society for the Study of Obesity. Multivariable Cox proportional-hazards models were used to calculate the hazard ratios (HRs) and 95% confidence intervals (CIs) for the future risk of abdominal obesity for each dietary pattern after adjusting for potential covariates. After an average follow-up of 4.89 years, we reported 5878 cases (1932 men and 3946 women) of abdominal obesity. Based on factor analysis, three major dietary patterns were identified in both men and women: the “healthy”, “coffee and sweets”, and “multi-grain” patterns. In the fully adjusted model, the “healthy” pattern was inversely associated with the incidence of abdominal obesity (HR for fourth vs. first quartile: 0.86; 95% CI: 0.75–0.98; p for trend = 0.0358 for men; HR for fourth vs. first quartile: 0.90; 95% CI: 0.83–0.99; p for trend = 0.0188 for women), whereas the “coffee and sweets” pattern was positively associated with it (HR for fourth vs. first quartile: 1.23; 95% CI: 1.08–1.40; p for trend = 0.0495 for men; HR for fourth vs. first quartile: 1.14; 95% CI: 1.04–1.25; p for trend = 0.0096 for women). In contrast, the “multi-grain” pattern in men and women showed no significant association with the incidence of abdominal obesity. Diets rich in colorful vegetables, seaweeds, mushrooms, tubers, fruits, soy products, and fish and low in coffee, sweets, and oils/fats might be favorable for reducing the future risk of abdominal obesity, particularly in middle-aged and older Korean adults. Full article

Background: Acute ileal diverticulitis is a rare disease mimicking acute appendicitis. Inaccurate diagnosis with a low prevalence and nonspecific symptoms leads to delayed or improper management. Methods: This retrospective study aimed to investigate the characteristic sonographic (US) and computed tomography (CT) findings with clinical features in seventeen patients with acute ileal diverticulitis diagnosed between March 2002 and August 2017. Results: The most common symptom was abdominal pain (82.3%, 14/17) localized to the right lower quadrant (RLQ) in 14 patients. The characteristic CT findings of acute ileal diverticulitis were ileal wall thickening (100%, 17/17), identification of inflamed diverticulum at the mesenteric side (94.1%, 16/17), and surrounding mesenteric fat infiltration (100%, 17/17). The typical US findings were outpouching diverticular sac connecting to the ileum (100%, 17/17), peridiverticular inflamed fat (100%, 17/17), ileal wall thickening with preserved layering pattern (94.1%, 16/17), and increased color flow to the diverticulum and surrounding inflamed fat on color Doppler imaging (100%, 17/17). The perforation group had a significantly longer hospital stay than non-perforation group (p = 0.002). In conclusion, acute ileal diverticulitis has characteristic CT and US findings that allow radiologists to accurately diagnose the disease. Full article

The cage-free system has gained a lot of interest in recent years because it can offer chickens more freedom and is easier to manage compared with free-range rearing systems, but few studies have focused on the effect of the cage-free rearing system on meat quality and flavor. In this study, 44 Jianghan chickens were reared in caged or cage-free systems to explore the effect of different rearing systems on meat-eating quality. Sensory evaluation of cooked muscles showed that the leg muscle aroma, juiciness, and flavor intensity significantly improved by the cage-free rearing. The cage-free hens had significantly lower body weight, abdominal fat percentage, and meat fat content, but higher meat moisture content. The cage-free group had brighter breast muscle and redder leg muscle color 24 h after slaughter. Transcriptomic and metabolomic profile analysis of the leg muscle samples showed that the cage-free rearing changed biosynthesis pathways associated with glycogen metabolism, lipid and fatty acid biosynthesis and transport, muscle cellular type, and cellular components, which were related to raw meat quality. Different rearing systems also resulted in differences in glycolipid metabolism, lipid metabolism, and altered levels of intramuscular fat content and other flavor precursors. Pathways such as glycerolipid metabolism, adipocytokine signaling, and metabonomic pathways such as linoleic acid, glycerophospholipid, arginine, proline, and β-alanine metabolism may be responsible for the meat quality and flavor change. Full article

This study was to determine the effect of the organic raising system (OR) on growth performance, meat quality, and physicochemical properties of slow-growing chickens. Three hundred and sixty (one-day-old) Korat chickens (KRC) were randomly assigned to control (CO) and OR groups. The groups comprised six replicates of thirty chickens each. The chickens were housed in indoor pens (5 birds/m²), wherein those in OR had free access to Ruzi pasture (1 bird/4 m²) from d 21 to d 84 of age. In the CO group, chickens were fed with a mixed feed derived from commercial feedstuffs, while those in the OR group were fed with mixed feed derived from organic feedstuffs. The results revealed a lower feed intake (p < 0.0001) and feed conversion ratio (p = 0.004) in the OR. The OR increased total collagen, protein, shear force, color of skin and meat, and decreased abdominal fat (p < 0.05). The OR improved fatty acid with increased DHA, n-3 PUFA, and decreased the ratio of n-6 to n-3 PUFA in KRC meat (p < 0.05). The synchrotron radiation-based Fourier transform infrared spectroscopy and correlation loading analyses confirmed these results. In conclusion, our results proved that OR could improve growth performance and meat quality and suggested the raising system be adopted commercially. In addition, the observed differences in biochemical molecules could also serve as markers for monitoring meat quality. Full article

This trial was performed to determine the effect of rice bran oil (RBO) inclusion in diets of broiler chickens on performance, carcass characteristics, blood parameters, meat quality, antioxidant activity, liver lipid content, and liver histological structure. The 35-day feeding trial was conducted on 240 one-day-old Ross 308 broiler chickens, allocated to four treatment groups with six replicates each. RBO was examined at different inclusion levels, 0% (control), 1% (RBO_1%), 1.5% (RBO_1.5%), and 2% (RBO_2%) in a completely randomized design. The results showed that at the end of the trial (35 days) the RBO supplementation had positive effects (p < 0.001) on the productivity parameters, but the feed intake was linearly decreased due to RBO inclusion. In addition, RBO supplementation linearly improved (p < 0.05) the dressing percentage, breast yield, immune organs relative weights, and meat glutathione concentration, while it decreased (p < 0.01) the abdominal fat yield and meat crude fat, triglycerides, cholesterol, and Malondialdehyde (MDA) contents in broiler’s meat. Moreover, serum total protein, globulin, and high-density lipoprotein contents improved noticeably (p < 0.01) due to offering an RBO-supplemented diet, but serum total lipids, total cholesterol, triglyceride, low-density lipoprotein, and aspartate aminotransferase concentrations linearly reduced (p < 0.01). The RBO supplementation augmented (p < 0.05) the phagocytic index, phagocytic activity, and antibody titer compared to control. On the other hand, RBO inclusion had no effect on the breast, thigh, or abdominal fat color parameters. Moreover, RBO supplementation reduced (p < 0.01) the content of total saturated FA (SFA), but increased (p < 0.01) the content of total monounsaturated FA (MUFA), and polyunsaturated FA in both breast and thigh meat. Chemical analysis of the liver tissue samples revealed that the inclusion of RBO linearly reduced (p < 0.05) hepatic cholesterol, triglyceride, and MDA contents. Histologically, the lipid percentage and number of lipid droplets (p < 0.01) were markedly lessened in the RBO-supplemented groups. The histological structure of the liver asses by light and electron microscope were normal in all groups without any pathological lesions. It is concluded that RBO could be used as a valuable ingredient in broiler chickens’ diets to stimulate the growing performance and immune status, enhance the antioxidant activity and blood lipid profile, augment liver function, and improve the nutritive value of the meat. Full article