Search Results (459)

Pistachio (Pistacia vera) is widely consumed among tree nuts but capable of triggering severe IgE-mediated reactions in allergic individuals. Due to the similarity of cashew-borne and pistachio-borne allergen proteins and DNA, traditional detection methods, such as ELISA and PCR, often suffer from cross-reactivity, limiting their ability to discriminate between these two allergens. This study presents a sensitive LC-MS/MS method for the simultaneous detection of pistachio and cashew allergens in processed food with a screening detection limit (SDL) equal to 1 mg/kg. The method was validated for specificity, SDL, β error, precision, and ruggedness, and applied to various matrices (cereals, chocolate, sauces, and meat products). Ruggedness testing showed that all considered parameters must be carefully monitored by the operator, and sample preparation must be carried out without any modification in parameter values, under strictly controlled conditions. Good reproducibility was achieved for pistachio detection, while ongoing investigations should be carried out to overcome existing constraints for cashew. The LC-MS/MS method described in this work is a discriminatory method suitable for official food allergen control to selectively differentiate pistachio from cashew allergens, overcoming the limitations of PCR and ELISA when cross-reactivity occurs. It represents a validated tool for pistachio detection and a promising approach toward improving cashew allergen analysis. Full article

Soils are critical natural resources, yet their abiotic contributions to ecosystem services remain largely unexplored in valuation studies. This pilot study represents, to the best of our knowledge, the first attempt to assess the perceived value of geosystem services (GSs) from a consumer perspective. Using a discrete choice experiment with 200 respondents, we evaluated preferences for Rotella apples and Moscatello wine through mixed multinomial logit and latent class models. Results show that attributes related to soil use and soil control were consistently significant drivers of consumer utility (e.g., odds ratios of 9.38 and 5.78 for Moscatello wine and 8.46 and 5.56 for Rotella apples, respectively; p < 0.01). These attributes align more closely with the concept of a “geological fingerprint” than with existing geographical labeling schemes such as the Protected Designation of Origin. Price effects were statistically insignificant, indicating virtually no influence on choices. Both estimated models revealed preference heterogeneity and a substantial number of no-buy responses. This suggests both limited consumer familiarity with GS concepts and a limitation of our attribute descriptions, which likely failed to convey information needed for effective purchasing decisions. This study is exploratory and limited by its convenience sample, imperfect price specification, and inability to estimate willingness-to-pay measures. Nevertheless, it provides empirical support for introducing geological footprint labeling and highlights the need for improved consumer information, policy tools, and public campaigns to promote recognition and sustainable management of geodiversity in agriculture. Full article

The development of biomaterials with tailored properties is indispensable for biomedical applications. In this study, amorphous silica/sodium alginate (SiO₂/SA) hybrids were synthesized via the sol–gel method by incorporating 2, 5, and 8% sodium alginate into the silica matrix. The hybrids were characterized to evaluate their structural, surface, thermal, moisture-responsive, and biological properties. FTIR and XRD analyses confirmed the formation of organic–inorganic networks and amorphous structures. BET measurements revealed a specific surface area of 325 m²/g for SiO₂/SA2%, decreasing with higher SA content to 104.3 m²/g for SiO₂/SA8%; the moisture sorption capacity followed a similar trend. Thermal analysis indicated improved stabilization of the polymer within the silica matrix. Cytotoxicity tests on HaCaT (human keratinocyte) cells line revealed moderate toxicity for the SiO₂/SA2% hybrid (~40% cell viability inhibition (CVI)), while increasing the SA content reduced cytotoxicity, with a CVI of 33% for SiO₂/SA5% and ~15% for SiO₂/SA8%, all within non-toxic ranges according to ISO standards. The SiO₂/SA5% hybrid demonstrated the best balance between functional properties and biocompatibility. These preliminary results suggest that further optimization with intermediate SA concentrations (e.g., 6–7%) could further reduce cytotoxicity while maintaining desirable properties, supporting the potential of silica/sodium alginate hybrids in future biomedical applications. Full article

Urban wastewater is composed of nutrients such as nitrogen and phosphorus, organic matter, heavy metals, pathogens, and micropollutants. If untreated, these contribute to eutrophication and environmental degradation. Microalgae-based bioremediation offers a sustainable solution, showing promise for pollutant removal and high-value bioproduct generation. This study evaluates the efficacy of Galdieria sulphuraria ACUF 427 in treating urban wastewater, with a focus on nutrient removal and phycocyanin production at different optical densities (OD 2, OD 4, and OD 6). Nutrient removal rates (RRs) were analysed for ammonium nitrogen (N-NH₄⁺), ammonia nitrogen (N-NH₃), phosphate phosphorus (P-PO₄³⁻), and chemical oxygen demand (COD). The RR for N-NH₄⁺ increased with optical density, reaching 7.49 mg/L/d at an optical density of 6. Similar trends were observed for N-NH₃ and P-PO₄³⁻, with peak removal at OD 6. COD removal remained high across all ODs, though differences between OD 4 and OD 6 were not statistically significant. Significant variations (p < 0.05) in nutrient removal were noted across the ODs, except for COD between OD 4 and OD 6. Biomass growth and phycocyanin production were significantly higher in the wastewater compared to the control (Allen Medium), with the most effective performance observed at an optical density (OD) of 6. Maximum growth rates were 0.241 g/L/d at OD 6, 0.178 g/L/d at OD 4, and 0.120 g/L/d at OD 2. These results highlight the potential of G. sulphuraria as an agent for wastewater bioremediation and the production of high-value compounds, particularly at elevated cell densities, where we achieved superior nutrient removal and biomass production. Full article

Carbon dots (CDs) are gaining significant attention as multifunctional nanomaterials due to their optical properties, aqueous dispersibility, redox activity, and overall biocompatibility. This review presents a critical overview of the recent advances concerning the application of CDs in nucleic acid-centered diagnostics, with a specific focus on oxidative DNA damage. The use of CDs for the detection of oxidative DNA damage biomarkers, such as 8-oxo-2′-deoxyguanosine (8-oxo-dG), and their potential roles as fluorescent probes in environments related to oxidative stress is discussed in detail. The relationship between surface functionalization and biological performance is examined, highlighting how physicochemical properties dictate both the beneficial and adverse biological responses to CDs. Remarkably, CDs can act as antioxidants, mitigating oxidative damage, or as pro-oxidants, inducing cytotoxic effects, an ambivalent behavior that can be strategically harnessed for cytoprotection or selective tumor cell killing. Overall, this review outlines how CDs can contribute to the development of precision tools for studying oxidative environments affecting nucleic acids, with important implications for both diagnostics and redox-based therapeutic strategies of human diseases. Full article

Unmanned Aerial Vehicles (UAVs), commonly known as drones, are becoming increasingly important in multiple areas and various applications, including communication, detection, and monitoring. This review paper examines the development of antennas for UAVs, with a particular focus on miniaturization techniques, polarization strategies, and beamforming solutions. It explores both structural and material-based methods, such as meander lines, slots, high-dielectric substrates, and metasurfaces, which aim to make the antenna more compact without compromising performance. Different antenna types including dipole, monopole, horn, vivaldi, and microstrip patch are explored to identify solutions that meet performance standards while respecting UAV constraints. In terms of polarization strategies, these are often implemented in the feeding network to achieve linear or circular polarization, and beamforming techniques like beam-steering and beam-switching enhance communication efficiency by improving signal directionality. Future research should focus on more lightweight, structurally integrated, and reconfigurable apertures that push miniaturization through conformal substrates and programmable metasurfaces, extending efficient operation from 5/6 GHz into the sub-THz regime and supporting agile beamforming for dense UAV swarms. Full article

This study presents a wireless, non-invasive sensing system for monitoring the dielectric permittivity of materials, with a particular focus on applications in cultural heritage conservation. The system integrates a passive split-ring resonator tag, electromagnetically coupled to a compact antipodal Vivaldi antenna, operating in the reactive near-field region. Both numerical simulations and experimental measurements demonstrate that shifts in the antenna’s reflection coefficient resonance frequency correlate with variations in the dielectric permittivity of the material under test. A calibration curve was established using reference materials—including low-density polyvinylchloride, polytetrafluoroethylene, polymethyl methacrylate, and polycarbonate—and validated through precise permittivity measurements. The system was subsequently applied to wood samples (fir, poplar, beech, and oak) at different humidity levels, revealing a sigmoidal relationship between moisture content and permittivity. The behavior was also confirmed using a portable and low-cost setup, consisting of a point-like coaxial sensor that could be easily moved and positioned as needed, enabling localized measurements on specific areas of interest of the sample, together with a miniaturized Vector Network Analyzer. These results underscore the potential of this portable, contactless, and scalable sensing platform for real-world monitoring of cultural heritage materials, enabling minimally invasive assessment of their structural and historical integrity. Moreover, by enabling the estimation of moisture content through dielectric permittivity, the system provides an effective method for early detection of water-induced deterioration in wood-based heritage items. This capability is particularly valuable for preventive conservation, as excessive moisture—often indicated by permittivity values above critical thresholds—can trigger biological or structural degradation. Full article

Ricin, a type 2 ribosome-inactivating protein, is a lethal toxin found in castor bean seeds. Although the systemic toxicity of ricin has been extensively studied, its localized effect on the gastrointestinal tract remains a critical concern, particularly in the case of oral ingestion. This study investigates the cytotoxic effects of ricin on human intestinal epithelial cell lines and its impact on epithelial barrier integrity. Ricin cytotoxicity was assessed on the intestinal-derived HT29 and Caco-2 cell lines using dose– and time–response assays, while the epithelial integrity was evaluated via Trans-Epithelial Electrical Resistance (TEER) measurements in Caco-2 monolayers. Cell death was determined through flow cytometry analysis, and the protective effects of cell death inhibitors and antioxidant scavengers were investigated on ricin-intoxicated cells. Ricin showed high cytotoxicity on HT29 and Caco-2 cells, with EC₅₀ values in the nM range after 24–72 h of intoxication. Moreover, ricin strongly reduced TEER values in Caco-2 cells at 0.1–1 nM after 24 h of treatment. At a 1 nM concentration, ricin cytotoxicity can be significantly prevented by pre-incubating cells with the cell death inhibitors Z-VAD or necrostatin-1 and the antioxidant scavenger catalase, butylated hydroxyanisole or sodium pyruvate, demonstrating the involvement of apoptosis/necroptosis and oxidative stress in ricin cell death pathways and mechanisms. Full article

The adoption of technological innovations in the maritime industry is of interest to business, policy, and academic communities. In the last group, this interest has translated into the publication of a large but scattered literature, making it difficult to compare findings and identify the dynamics, structures, and patterns that might inform future research. A comprehensive review of past research on this topic might help achieve this. To date, no such review has been carried out, which is an important gap in the literature that this paper contributes to bridging. Two bibliometric review techniques—co-citation analysis of cited references and bibliographic coupling of documents—are applied to 171 journal articles published between 1999 and February 2025 to answer the following questions: 1. What is the knowledge base of this literature? 2. What are the recent research trends (research fronts) in this literature? The analysis reveals that research on “shore power” dominates both the knowledge base and research fronts. Other key research themes centre on “autonomous shipping”, “blockchain”, and “alternative fuels”. Based on these results, implications for future research are drawn. Full article

Isotopic analyses are useful tools with a wide range of applications, including environmental studies, archaeology and biomedicine. Founded in 2019 at the University of Campania “Luigi Vanvitelli”, the iCONa laboratory specialises in stable isotope mass spectrometry, with a particular focus on cultural heritage. The laboratory performs carbon, nitrogen and oxygen isotopic analyses, including the most recent advances in compound-specific stable isotope analysis of amino acids (CSIA-AAs). In addition to these analytical services, iCONa provides chemical and physical sample preparation for a variety of sample types. This paper focuses on our applications in the field of cultural heritage, exploring how the analysis of stable isotopes performed on archaeological remains can be used to reconstruct past subsistence strategies and human behaviours. We also discuss the challenges inherent in isotopic analysis and recent methodological advances in the field. Full article

Chronic Obstructive Pulmonary Disease (COPD) is a multifactorial condition associated with significant systemic complications such as cardiovascular disease (CVD), metabolic disorders, muscle wasting, and sarcopenia. While Body Mass Index (BMI) is a well-established indicator of obesity and has prognostic value in COPD, its role in predicting disease outcomes is complex. Muscle wasting is prevalent in COPD patients and exacerbates disease severity, contributing to poor physical performance, reduced quality of life, and increased mortality. Additionally, COPD is linked to metabolic disorders, such as dyslipidemia and diabetes, which contribute to systemic inflammation and worse prognosis and, therefore, should be treated. The systemic inflammatory response plays a central role in the development of sarcopenia. In this review, we highlight the mixed efficacy of statins in managing dyslipidemia in COPD, considering side effects, including muscle toxicity in such a frail population. Alternative lipid-lowering therapies and nutraceuticals, in addition to standard treatment, have the potential to target hypercholesterolemia, which is a coexisting condition present in more than 50% of all COPD patients, without worsening muscle wasting. The interference between adipose tissue and lung, and particularly the potential protective role of adiponectin, an adipocytokine with anti-inflammatory properties, is also reviewed. Respiratory, metabolic and muscular health in COPD is comprehensively assessed. Identifying and managing dyslipidemia and paying attention to other relevant COPD comorbidities, such as sarcopenia and muscle wasting, is important to improve the quality of life and to reduce the clinical burden of COPD patients. Future research should focus on understanding the relationships between these intimate mechanisms to facilitate specific treatment for systemic involvement of COPD. Full article

This study investigates the combined use of electron beam irradiation (EBI) and nanotechnology to develop improved food packaging films. EBI, commonly applied for sterilization, can alter polymer microstructure, while irradiated cellulose nanocrystals (CNCs) offer enhanced functionality when incorporated into biopolymer matrices. Here, CNCs were irradiated with doses up to 50 kGy, leading to the formation of carboxyl and aldehyde groups, confirmed by FTIR analysis, as a consequence of the initial formation of free radicals and peroxides that may subsist in that original form or be converted into various carbonyl groups. Flexible films were obtained by incorporating pristine and EB-irradiated CNCs in an internal mixer, using minute amounts of poly(ethylene oxide) (PEO) to facilitate the dispersion of the filler within the polymer matrix. The resulting PLA/PEO/CNC films were evaluated for their mechanical, thermal, barrier, and antioxidant properties. The results showed that structural modifications of CNCs led to significant enhancements in the performance of the composite films, including a 30% improvement in water barrier properties and a 50% increase in antioxidant activity. These findings underscore the potential of irradiated CNCs as effective additives in biopolymer-based active packaging, offering a sustainable approach to reduce dependence on synthetic preservatives and potentially extend the shelf life of food products. Full article

Membrane transporters are vital for solute movement and localisation across cellular compartments, particularly in extremophilic organisms such as Galdieriales. These red algae thrive in geothermal and metal-rich environments, where adaptive transporter systems contribute to their metabolic flexibility. While inventories of transporter genes in the species Galdieria sulphuraria have previously been compiled, their phylogenetic origins remain incompletely resolved. Here, we conduct a comparative phylogenetic analysis of three transporter families—Major Facilitator Superfamily (MFS). Amino acid–Polyamine–Organocation (APC) and the natural resistance–associated macrophage protein (Nramp)—selected from overexpressed transcripts in G. sulphuraria strain SAG 107.79. Using sequences from six Galdieriales species and orthologs from diverse taxa, we reconstructed maximum likelihood trees to assess conservation and potential horizontal gene transfer (HGT). The MFS subfamilies revealed contrasting patterns: sugar porters (SPs) exhibited polyphyly and fungal affinity, suggesting multiple HGT events, while phosphate:H⁺ symporters (PHSs) formed a coherent monophyletic group. APC sequences were exclusive in G. sulphuraria and extremophilic prokaryotes, indicating a likely prokaryotic origin. In contrast, Nramp transporters were broadly conserved across eukaryotes and prokaryotes, showing no signs of recent HGT. Together, these findings highlight the mosaic evolutionary history of membrane transporters in Galdieriales, shaped by a combination of vertical inheritance and taxon-specific gene acquisition events, and provide new insight into the genomic strategies underpinning environmental resilience in red algae. Full article

The aim of this study was to evaluate the efficiency of activated biochar produced from Moringa oleifera L. wood for removing azoxystrobin (fungicide) and deltamethrin (insecticide) from water. The adsorption of pesticides on activated carbon was studied using batch tests evaluating the influence of contact time (1–180 min), concentration (5–50 mg L⁻¹), and temperature (283, 298 and 313 K). The highest removal percentage obtained was 94.39% for azoxystrobin and 91.96% for deltamethrin, considering an initial concentration of 10 mg L⁻¹ and adsorbent dosage of 5.0 g L⁻¹. FTIR spectra confirmed H-bonding in the adsorption process, SEM analysis revealed homogeneous surface area characteristics, and BET results confirmed a highly superficial area for the activated carbon, all of which favor pesticide adsorption. The Boyd model showed that the intraparticle diffusion stage is determinant for both compounds in the initial stages of the adsorption process. The Sips model was the isotherm with the best fit to the experimental data, possibly indicating cooperativity between adsorbate molecules at low temperatures. The thermodynamic study showed a favorable adsorption at all the temperatures investigated, given the negative value of ΔG°. In addition, this study revealed good adsorption capacities for the material indicating that Moringa oleifera wood activated carbon is a viable alternative for removing azoxystrobin and deltamethrin from water. Full article

‘Asprinio’ grape is used to produce a white wine from the Campania region, known as ‘Asprinio’ (DOC since 1993). A plethora of approaches was adopted to characterize the organic compounds (e.g., free amino acids and other metabolites) and microbial population (bacteria and fungi) in ‘Asprinio’ grape must by collecting samples from three different vineyards cultivated with the ‘alberata’ training system. The average free amino acid content of ‘Asprinio’ grape must showed quantitative variations, but no significant statistical differences were found. On average, proline was the most abundant free amino acid (~282 mg/L; 47.9%), followed by arginine (~66 mg/L; 11.5%) and glutamine (~25.2 mg/L; 4.2%). On the other hand, the total polyphenol content (TPC) of ‘Asprinio’ grape must was different, like their antioxidant activity, which increased when the TPC content was higher. Moreover, 1D and 2D NMR spectra highlighted the presence of high sugars amount (in particular glucose) as well as trans-caftaric acid, trans-coutaric acid, trans-fertaric acid, and the cis-isomers of these cinnamate esters. Finally, the evaluation of the microbial communities in the ‘Asprinio’ grape must revealed the presence of several representative bacterial phyla—mainly Bacteroidota, Proteobacteria, and Actinobacteriota—as well as various fungal genera, including Cladosporium, Hanseniaspora, Aspergillus, and Saccharomyces. Notably, these microorganisms, which contribute to the fermentation process and influence the final wine flavor, have been found in different proportions in the grape musts analyzed. Our results contribute to increasing knowledge of the ‘Asprinio’ grape, an indigenous vine of Southern Italy. Full article