Search Results (48)

Identifying the key products of mopane woodlands, the perceived abundance of species, and the associated use values have important implications for rural communities and the sustainable use of mopane woodlands. This study examined local perceptions of tree species abundance and their associated use-values across four communities in the Mopani District, South Africa. The semi-structured questionnaire and key informant interviews were used to collect the data. The data were analyzed using descriptive statistics, the Friedman test, the Smith Salience Index, Kendall’s W, Pearson correlation, and the Fidelity test. There was a very strong level of agreement (W = 0.83) among the communities regarding the perceived abundance of tree species, and this agreement was statistically significant (χ² = 19.85, p < 0.05). The Colophospermum mopane, Sclerocarya birrea, and Combretum imberbe are the most perceived abundant species in the study areas and have high value. The tree species with high use-value showed a positive correlation with the perceived abundance of tree species, conforming to the study’s ecological apparency hypothesis. The more apparent species had more use value and perceived abundance. Full article

The development of tropical maize populations with high heterosis potential is essential for sustaining genetic progress in hybrid breeding programs, yet accurate selection remains challenging due to genotype–phenotype interactions and inbreeding depression. This study evaluated the efficiency of five selection strategies in recurrent selection programs using F₂ populations derived from commercial maize hybrids: Smith–Hazel Index (SHI), Base Index (BIA), Mulamba–Mock Index (MMI), REML/BLUP for grain yield (BLUP_GY), and REML/BLUP for inbreeding depression (BLUP_ID). Consistency among methods was assessed with a heatmap, and predicted genetic gains were compared with realized field performance. Predicted gains were highest with MMI and BIA for grain yield and ear weight, although realized results revealed discrepancies, particularly for BLUP-based approaches. Notably, BLUP_GY, which had the lowest predicted yield (4025 kg ha⁻¹), achieved a realized yield of 5620 kg ha⁻¹, surpassing BIA and SHI. This indicates that additive potential was underestimated in predictions, likely due to dominance and environmental effects in early F₂ cycles. Overall, BLUP-based methods proved effective in identifying progenies with higher additive value, and their integration with phenotypic indices is recommended to combine short-term realized gains with sustained genetic improvement. Full article

Breeding programs often overlook the use of root traits. Therefore, we investigated the relevance of sorghum root traits in explaining its adaptation to combined drought and heat stress (CDHS). Six (i.e., three pre-release lines + three checks) sorghum genotypes were established at two low-altitude (i.e., <600 masl) locations with a long-term history of averagely very high temperatures in the beginning of the summer season, under two management (i.e., CDHS and well-watered (WW)) regimes. At each location, the genotypes were laid out in the field using a randomized complete block design (RCBD) replicated two times. Root trait data, namely root diameter (RD), number of roots (NR), number of root tips (NRT), total root length (TRL), root depth (RDP), root width (RW), width–depth ratio (WDR), root network area (RNA), root solidity (RS), lower root area (LRA), root perimeter (RP), root volume (RV), surface area (SA), root holes (RH) and root angle (RA) were gathered using the RhizoVision Explorer software during the pre- and post-flowering stage of growth. RSA traits differentially showed significant (p < 0.05) correlations with grain yield (GY) at pre- and post-flowering growth stages and under CDHS and WW conditions also revealing genotypic variation estimates exceeding 50% for all the traits. Regression models varied between pre-flowering (p = 0.013, R² = 47.15%, R² Predicted = 29.32%) and post-flowering (p = 0.000, R² = 85.64%, R² Predicted = 73.30%) growth stages, indicating post-flowering as the optimal stage to relate root traits to yield performance. RD contributed most to the regression model at post-flowering, explaining 51.79% of the 85.64% total variation. The Smith–Hazel index identified ICSV111IN and ASAREACA12-3-1 as superior pre-release lines, suitable for commercialization as new varieties. The study demonstrated that root traits (in particular, RD, RW, and RP) are linked to crop performance under CDHS conditions and should be incorporated in breeding programs. This approach may accelerate genetic gains not only in sorghum breeding programs, but for other crops, while offering a nature-based breeding strategy for stress adaptation in crops. Full article

The Brazilian semi-arid Northeast plays a critical role in regional hydrology, where rainfall is marked by pronounced temporal variability and short duration, presenting significant challenges for understanding and managing hydrological and erosive processes. This study aims to evaluate the performance of empirical models for estimating rainfall kinetic energy (KE) and erosivity index (EI₃₀) in this region, for all events and erosive events, using high-resolution rainfall data collected at the Federal University of Cariri (UFCA), Ceará. A total of 283 natural rainfall events were analyzed, with KE and EI₃₀ values calculated using multiple models: Wischmeier and Smith, USDA, Van Dijk, a temporal variation-based model (KE_VT), and a regional model developed for Brazil’s semi-arid zone, which served as the reference. The results show a predominance of small rainfall events (<5.2 mm), though maximum EI₃₀ values exceeded 1300 MJ ha⁻¹ mm h⁻¹, highlighting the potential for extreme erosive events. Comparative analysis revealed that all international models significantly underestimated KE and EI₃₀ values compared to the regional reference, with the KE_VT model showing the closest approximation (13% underestimation), for all events and erosive events. Statistical assessments using the Wilcoxon test, Nash–Sutcliffe efficiency, and Willmott concordance index confirmed the superior performance of the KE_VT, for all events and erosive events. These findings underscore the importance of considering intra-event rainfall variability and regional calibration when modeling erosivity in semi-arid climates, contributing to more effective soil conservation and hydrological planning. Full article

In recent decades, honeybee populations have declined, dramatically owing to destructive honey harvesting practices and the loss of foraging grounds and nesting sites. Among them, Apis laboriosa Smith, 1871 (Hymenoptera, Apidae), an important pollinator species found in the Himalayan region, holds significant economic and ecological value. However, conservation efforts and intraspecific taxonomic studies regarding it have been rather limited, and thus its full geographic range remains elusive. This study is the first to research A. laboriosa in Sichuan. Through a systematic study integrating morphological feature analysis and genomic data, the following conclusions are drawn. Whole-genome resequencing data analysis reveals that the Sichuan population forms a new monophyletic group (Bootstraps = 100). In the past ten thousand years, the population sizes of A. laboriosa in four different regions of China have been decreasing rapidly. Measures should be taken to protect them across the entire distribution range, especially the populations in Tibet and Sichuan, due to their relatively large genetic differences and low intra-population genetic diversity. Based on the significant difference analysis, the following four wing vein morphological features with extremely significant differences were identified: the width of the right forewing (FB), the cubital index a/b (Ci), the forewing vein angle (E9), and the forewing vein angle (K19). These findings are expected to offer a valuable reference for future A. laboriosa conservation endeavors, particularly in protecting populations with a high level of genetic differentiation. Full article

The optimal harvest date (OHD) for the long-term storage of apple fruits is of the utmost importance, not only for maintaining high quality levels, but also because the ripening stage, regulated by the autocatalytic activity of the internal ethylene concentration, greatly affects the VOCs’ synthesis. During apple ripening, chemical compounds undergo changes that affect the fruit’s overall quality, particularly its aromatic profile. Three main classes of organic molecules—aldehydes, alcohols, and esters—play a key role in these modifications. This study investigated the potential of proton transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS) for the rapid, non-destructive monitoring of VOC profiles in ‘Red Delicious’ and ‘Granny Smith’ apples over a 7-week shelf-life period across three harvest dates with different ripening stages. More than 300 mass peaks in the PTR-ToF-MS spectra of the apple headspace were detected. A total of 127 of them were considered to be relevant for further analysis. Furthermore, respiration rate and I_AD index were used for the non-destructive assessment of the ripening progress during the 7 weeks of shelf-life and for integrating the VOC results. Full article

Most of the processes with various dynamic characteristics can be reduced to the Second Order Plus Time Delay (SOPTD) model by using the model reduction method. We propose a novel hybrid approach that combines Long Short-Term Memory (LSTM)-based real-time model identification with Genetic Algorithms to enhance the Smith predictor control structure. This method compensates for the delay time of the SOPTD model while minimizing the Integral Time Absolute Error performance index. Our approach integrates an optimally adaptive Proportional–Integral–Derivative (PID) controller design algorithm that estimates the coefficients of the SOPTD model in the Smith Predictor control structure and adjusts the PID controller parameters dynamically. The method is improved through a combination of numerical calculation, Genetic Algorithms, and LSTM networks, showing approximately 15% better performance compared to conventional methods. The system demonstrates significant improvements in both performance metrics and resource utilization, including a 40% reduction in execution time and enhanced resource efficiency. Simulation results show that the proposed scheme exhibits improved adaptability to disturbances and process variations, with faster response times and reduced overshoots compared to traditional methods. The steady-state response of the higher-order model and the reduced model shows perfect matching for the unit feedback input. Full article

Background/Objectives: To assess the feasibility and acceptability of freelisting for adolescents with chronic musculoskeletal pain (CMP) and use freelisting to identify how adolescents with CMP cope with pain. Methods: This was a mixed-methods cross-sectional single-center study of patients 12–18 years old, diagnosed with CMP. Twenty-seven subjects participated in an interview which included the freelisting exercise, probing questions, Connor-Davidson Resilience Scale 10-item, and semi-structured interview. Feasibility was pre-defined as ≥85% completion. A list of ‘standardized’ freelisting terms was created, and we calculated Smith’s salience index. Freelisting terms were grouped into individual, family, friends, school, and medical domains. Results: In this predominantly female population, resilience levels were low to moderate, and on average, participants had moderate pain intensity and functional disability. The freelisting exercise was feasible and acceptable among females, with low recruitment of males. Salient words to describe adolescents’ pain included frustrating, upsetting, annoying, and painful. Participants identified family, friends, teachers, guidance counselors, and health professionals as key sources of support. Many participants reported difficulties communicating their pain. Helpful coping strategies included rest, distraction, relaxation, endurance, and extracurricular participation. Conclusions: Freelisting was well perceived among female adolescents with CMP. Supportive relationships with community members play an important role for this population. However, perceived stigma may deter female adolescents from talking about their pain. Purposive sampling of male participants and recruitment strategies ensuring diverse patient representation are necessary to ensure generalizability of future results when using the freelisting method for adolescents with CMP. Full article

Background/Objectives: The increasing prevalence of video gaming has raised concerns about its potential impact on musculoskeletal health, particularly temporomandibular disorders (TMDs). This study aims to compare TMD symptoms, mandibular function, and dental wear between gamers and non-gamers among university students. Methods: An observational study included 108 students aged 20 to 23 years, divided into gamers (n = 48) and non-gamers (n = 60). Participants completed questionnaires assessing TMD symptoms, gaming habits, and screen time. Clinical examinations measured mandibular movements, palpation-induced pain, and dental wear using the Smith and Knight Tooth Wear Index. Statistical analyses included independent t-tests, chi-square tests, Pearson’s correlations, and logistic regression. Seven comprehensive tables present the findings with p-values. Results: Gamers reported significantly higher screen time (Mean = 6.5 h/day) compared to non-gamers (Mean = 4.0 h/day; p < 0.001). Maximum unassisted mouth opening was greater in gamers (Mean = 48.31 mm) than in non-gamers (Mean = 46.33 mm; p = 0.04). Gamers exhibited a higher prevalence of pain on palpation of the masseter muscle (45.8% vs. 30.0%; p = 0.05). Dental wear scores were significantly higher in gamers for teeth 2.3 (upper left canine) and 3.3 (lower left canine) (p < 0.05). Positive correlations were found between hours spent gaming and maximum mouth opening (r = 0.25; p = 0.01) and dental wear (r = 0.30; p = 0.002). Logistic regression showed that gaming status significantly predicted the presence of TMD symptoms (Odds Ratio = 2.5; p = 0.03). Conclusions: Gamers exhibit greater mandibular opening, increased dental wear, and a higher prevalence of masticatory muscle pain compared to non-gamers. Prolonged gaming may contribute to altered mandibular function and increased risk of TMD symptoms. Further research is needed to explore underlying mechanisms and develop preventive strategies. Full article

Rivers are crucial hydrological cycle components, supporting ecosystems and human activities. Managing and protecting river water quality is essential. Diatoms, microscopic algae, are widespread and sensitive to changes in water quality, making them effective bioindicators. This study focused on the Kuils River in the Western Cape, South Africa, where diatom sampling was conducted at four sites. Their communities are affected by different physicochemical parameters, such as changes in pH, salinisation, eutrophication, and organic enrichment. A total of 98 diatom species were identified. The Omnidia software was used to calculate the Generic Diatom Index, Specific Pollution Index, and Trophic Diatom Index. Historical physicochemical data such as pH, chemical oxygen demand, and phosphorus were obtained from the Department of Water and Sanitation between 2019 and 2021 and were used to provide a reference for comparing diatoms. The results showed clear signs of pollution, as shifts in diatom species composition were observed. Pollution-tolerant species such as the Nitzschia palea (Kützing) W Smith 1856, Navicula viridula (Kützing) Ehrenberg 1838, Eunotia bilunaris (Ehrenberg) Schaarschmidt 1880, and Ulnaria ulna (Nitzsch) Compère 2001 dominated, while less pollution-tolerant species like Gomphonema parvulum f.saprophilum Lange-Bertalot and E. Reichardt 1993 and Stephanocylus meneghinianus (Kutzing) Kulikovskiy, Genkal, and Kociolek 2022 were less abundant. Both diatom indices and physicochemical data indicated poor water quality in the Kuils River. The study concludes that diatoms are a valuable tool for biomonitoring river water quality and recommends their use alongside traditional physicochemical methods for future assessments of river systems. Full article

Purpose: We describe the impact of screening on outcomes of patients diagnosed with hepatocellular carcinoma (HCC) in an urban safety-net healthcare system compared to a non-screened cohort diagnosed with HCC. Methods: Patients diagnosed with HCC at John Peter Smith Health Network were identified by querying the hospital tumor registry and allocated to the screened cohort if they had undergone any liver imaging within one year prior to HCC diagnosis, while the remainder were allocated to the non-screened cohort. Kaplan–Meier methods and log-rank tests were used to compare 3-year survival curves from an index date of HCC diagnosis. Cox proportional hazard models were used to calculate unadjusted and adjusted hazard ratios (HRs) and 95% confidence intervals (CIs). The Duffy adjustment was used to address lead-time bias. Results: A total of 158 patients were included (n = 53 screened, n = 105 non-screened). The median overall survival (OS) for the screened cohort was 19.0 months (95% CI: 9.9–NA) and that for the non-screened cohort was 5.4 months (95% CI: 3.7–8.5) [HR death (non-screened vs. screened) = 2.4, 95% CI: 1.6-3.6; log rank p < 0.0001]. The benefit of screening remained after adjusting for lead-time bias (HR 2.19, 95% CI 1.4–3.3, p = 0.0002). Conclusions: In an urban safety-net population, screening for HCC was associated with improved outcomes compared to patients diagnosed with HCC outside of a screening protocol. Full article

This study aimed to monitor the oxygenation and blood supply in three quadriceps muscles [the vastus lateralis (VL), vastus medialis (VM), and rectus femoris (RF)] during squatting exercise to exhaustion. Eighteen young resistance-trained males performed five sets of 15 back squats in a Smith machine, with two warm-up sets [at 14% and 45% of the 15-repetition maximum (15RM)] and three main sets at 100% of the 15RM. Three near-infrared spectroscopy devices were attached to the VL, VM, and RF to record the muscle oxygen saturation (SmO₂) and total hemoglobin (tHb, an index of muscle blood supply). The blood lactate concentration was measured after each set with a portable analyzer. The SmO₂ and tHb data were analyzed by repeated-measures two-way ANOVA (muscle × set). Lactate data were analyzed by repeated-measures one-way ANOVA. The statistical significance was set at α = 0.05. The SmO₂ dropped during each set (hitting zero in many instances) and was reinstated during recovery. The three main sets caused severe deoxygenation in the VL and VM, as opposed to moderate deoxygenation in the RF. From one set to the next, the initial value and the drop in the SmO₂ increased, whereas the final SmO₂ value decreased. The tHb increased in the VL, did not change considerably in the VM, and decreased in the RF during each set. The blood lactate concentration increased gradually from one set to the next, reaching about 10 mmol/L. These findings show pronounced differences in the physiological and metabolic responses of three quadriceps muscles to squatting exercise, thus highlighting the importance of studying such responses at multiple sites. Full article

Epidemiological, experimental, and ecological data have indicated the controversial effect of in utero chronic low dose rate (<6 mGy/h) with accumulative low (≤100 mGy) or high (>100 mGy) dose radiation exposure. Our main goal of this study was to examine if different low dose rates of chronic pre- and/or post-natal radiation exposure with accumulative high doses could induce hippocampal cellular, mRNA, and miRNA changes leading to neuropsychiatric disorders. The comprehensive mouse phenotypic traits, organ weight, pathological, and blood mRNA and miRNA changes were also studied. Using different approaches including SmithKline, Harwell, Imperial College, Royal Hospital, Phenotype Assessment (SHIRPA), neurobehavioral tests, pathological examination, immunohistochemistry, mRNA and miRNA sequencing, and real-time quantitative polymerase chain reaction (qRT-PCR) validation, we found that in prenatally irradiated (100 mGy/d for 18 days with an accumulative dose of 1.8 Gy) 1-year-old mice, no cellular changes, including immature neurons in the subgranular zone, mature neurons and glial cells in the hilus of the dentate gyrus and development of cognitive impairment, neuropsychiatric disorders, occurred. However, a significant reduction in body weight and mass index (BMI) was indicated by the SHIRPA test. A reduced exploratory behavior was shown by an open field test. Organ weights showed significant reductions in the testes, kidneys, heart, liver and epididymides with no abnormal pathology. mRNA and miRNA sequencing and qRT-PCR validation revealed the upregulation of Rubcnl and Abhd14b, and downregulation of Hspa1b, P4ha1, and Banp genes in both the hippocampus and blood of mice prenatally irradiated with 100 mGy/d. Meanwhile, downregulation of miR-448-3p and miR1298-5p in the hippocampus, miR-320-3p, miR-423-5p, miR-486b-5p, miR-486b-3p, miR-423-3p, miR-652-3p, miR-324-3p, miR-181b-5p, miR-let-7b, and miR-6904-5p in the blood was induced. The target scan revealed that Rubcnl is one of the miR-181b-5p targets in the blood. We, therefore, concluded that prenatal chronic irradiation with a low dose rate of 100 mGy/d and accumulative dose of 1.8 Gy or below might not induce significant adverse health effects on the offspring. Further study of different low dose rate radiation exposures with accumulative high doses may provide threshold doses for authorities or regulators to set new radiation safety guidelines to replace those extrapolated from acute high dose/dose rate irradiation to reduce unnecessary emergency evacuation or spending once a nuclear accident or leakage occurs. Full article

Paris polyphylla Smith var. Chinensis (Franch.) Hara is a medicinal plant that belongs to the Liliaceae family. Its main components are parissaponins, which have excellent medicinal effects such as anti-inflammatory, anti-tumor, etc. Improving the quality of parissaponins through artificial directional regulation has emerged as a practice to meet medical demand and is a new research hotspot. In this paper, P. polyphylla plants were treated with UVA, UVB, and UVC, and the contents of PolyPhyllin VI (PPVI) and PolyPhyllin VII (PPVII), saponin synthase (squalene synthase, SS; cycloartenol synthase, CAS; cytochrome P450, CYP450; and glycosyl transferases, GT) activity, MDA, and the photosynthetic pigment indexes were measured and analyzed. The results showed that PPVII content increased by 32.43% with UVC treatment after 4 h (3.43 mg/g), but the PPVI and PPVII contents in the other groups decreased compared with CK (control group) and they did not return to the original level after 4 h. SS, CAS, CYP450, and GT synthases were activated in varying degrees via UV treatment and increased, respectively, by 22.93%, 10.83%, 20.15%, and 25.98%. Among them, GT, as the last of the synthetases, had a shorter response time to UVB (30 min) and UVC (15 min); the difference was sensible compared with CK. Moreover, UV had a stressing effect and promoted the rapid accumulation of MDA content (increased 17.66%, 34.53%, and 9.65%) and carotenoid (increased 7.58, 5.60, and 7.76 times) within 4 h compared to CK. UVB and UVC radiation visibly improved chlorophyll a content (42.56% and 35.45%), but UVA did not, and the change in chlorophyll b content showed no overt statistical difference. In addition, PPVI and PPVII were negatively correlated with SS, CAS, carotenoids, and MDA (p < 0.05) and positively correlated with CYP450, GT, and chlorophyll a (p < 0.05). This study provides a theoretical basis for using UV light to regulate secondary metabolism in P. polyphylla, which is of great value for production management. Full article

The color of an apple is an important index of its appearance quality, which affects the fruit’s marketability and value. The green variety “Granny Smith” remains green when ripe, and the pigment in the peel is mainly chlorophyll. The chlorophyll in the peel of the yellow variety “Golden Delicious” degrades as the fruit ripens. To explore the different mechanisms of chlorophyll degradation in the “Golden Delicious” and “Granny Smith” varieties, transcriptomic analysis was performed on the mature peels of both cultivars. High-quality sequences totaling 48,206,940 and 48,796,646 raw bases, as well as 9632 differentially expressed genes (DEGs), were identified. A total of 1206 DEGs were classified in 118 KEGG metabolic pathways. Additionally, 3258 transcription factors from 428 gene families were predicted, and DEGs associated with chlorophyll degradation, including MdSGR2, MdNYC1 and MdHCAR, were identified. Subsequently, the MdSGR2 gene was isolated from the mature peel of “Granny Smith” apples. Subsequent subcellular localization analysis confirmed that MdSGR2 specifically targets chloroplasts. The transient overexpression of MdSGR2 was found to negatively regulate chlorophyll degradation, leading to the inhibition or deceleration of chlorophyll breakdown, thereby maintaining green coloration in the leaves and peel. Conversely, silencing MdSGR2 positively regulated chlorophyll degradation, resulting in chlorosis in the leaves and peel. These findings offer valuable insights into the regulatory mechanism governing apple fruit coloration and lays a solid foundation for further investigation in this field. Full article