Search Results (39,094)

Age-related hyperphosphatemia is increasingly recognized as a contributing factor in sarcopenia. This work studies the metabolic effects of elevated phosphate on muscle. C2C12 cells were differentiated in the absence or presence of 10 mM β-glycerophosphate (BGP), an exogenous phosphate donor. In addition, quadriceps muscles from four experimental groups of male C57BL/6J mice were analyzed: young (5 months) and old (24 months) fed with standard diet; old mice fed with hypophosphatemic diet or supplemented with the phosphate binder Velphoro^®, for the last three months of life. Mice were stratified according to sarcopenia degree based on muscle mass, strength and physical performance. Protein levels were determined by immunoblotting and mRNA expression by RT-qPCR. ATP levels were measured by luminescence and L-lactate production, citrate synthase and cytochrome c oxidase activities by colorimetric assays. Mitochondrial content, membrane potential and reactive oxygen species (ROS) were determined by fluorescence assay. BGP-treated cells showed increased glucose transporter 1 (GLUT1) and decreased NADH Dehydrogenase (CI-NDUFB8) protein expression, elevated hexokinase II (HK2), phosphoglycerate kinase 1 (PGK1) and lactate dehydrogenase A (LDHA) mRNA levels, reduced ATP levels, increased lactate production, and decreased mitochondrial enzyme activities. Moreover, BGP increased ROS, diminished mitochondrial membrane potential, and altered fusion–fission dynamics and mitophagy. In aged quadriceps, oxidative phosphorylation (OXPHOS) subunits and superoxide dismutase 2 (SOD2) expression were reduced. The hypophosphatemic diet improved all parameters, whereas Velphoro^® selectively increased Mitochondrial cytochrome C oxidase subunit 1 (CIV-MTCO1) expression. Several altered mitochondrial markers are associated with sarcopenia degree. Altogether, hyperphosphatemia induces metabolic changes that scale with the sarcopenic degree. Our findings show a relevant association between hyperphosphatemia and mitochondrial dysfunction, and they support the potential benefit of phosphate reduction as a strategy to prevent or mitigate sarcopenia. Full article

This study examines the impact of ESG disclosure, leverage, and profitability on firm value, measured by Tobin’s Q, among 67 non-financial Tadawul-listed companies in Saudi Arabia over the period 2015–2024. ESG disclosure is captured through a manual content-analysis index that scores the proportion of expected environmental, social, and governance items reported by each firm. The study further investigates whether board independence moderates these relationships while controlling for liquidity, firm size, current ratio, capital expenditure, and board size. Methodologically, the study employs the two-step system generalized method of moments (system GMM) estimator, which addresses dynamic persistence, endogeneity, and unobserved heterogeneity. The findings reveal that ESG disclosure has a positive and significant effect on firm value, indicating that the Saudi market increasingly rewards firms that provide broader sustainability-related information. Profitability also exerts a positive influence on Tobin’s Q, while leverage has a negative and significant effect, suggesting that higher debt weakens market valuation. Among the moderating effects, board independence significantly reduces the negative impact of leverage on firm value, although it does not significantly strengthen the positive ESG disclosure–firm value relationship. The results also show that liquidity, firm size, capital expenditure, and board size positively influence firm value. The study’s novelty lies in being the first, to our knowledge, to integrate ESG disclosure, financial structure, profitability, and board independence within a single dynamic firm-value framework over a decade-long panel that brackets the Saudi Exchange’s 2021 ESG disclosure guideline. In doing so, it advances emerging-market ESG research by showing that, under Saudi Arabia’s largely voluntary disclosure regime and concentrated-ownership structure, board independence operates primarily as a risk-monitoring mechanism rather than as an amplifier of disclosure value. The findings imply that regulators should strengthen and progressively mandate ESG reporting frameworks, that investors should treat ESG transparency as value-relevant information, and that firms should view ESG transparency and prudent governance as strategic tools for enhancing market value in line with Vision 2030. Full article

Prostate cancer (PCa) is among the highest incidence malignancies in men, with high rates of inevitable resistance development, relapse, and mortality. Castration-resistant prostate cancer (CRPC) continued to pose substantial therapeutic challenges, highlighting the urgent need for effective treatment options. This study assessed the marine cembranoid sarcophine activity against the progression and recurrence of the metastatic CRPC (mCRPC) in mouse xenograft models. Protein and phosphorylation levels were assessed by immunoblotting and mRNA expression by qPCR and RNA sequencing. The in vivo efficacy was evaluated through tumor progression over 3 weeks followed by primary tumor excision and recurrence monitoring over an 8-week course. Sarcophine significantly reduced the mCRPC CWR-R1ca tumor volume by 74.1% and suppressed the epigenetic regulators EZH2 and SMYD2; lineage plasticity factors ASCL1 and BRN2; Wnt/stemness signaling markers β-catenin and LGR6; AKT total expression and activation; and invasion-associated proteins TRPC4 and MMP2 in primary tumors. Sarcophine effectively prevented the mCRPC locoregional recurrence, as well as lung and spleen distant recurrences, and effectively reduced recurrence in other organs. Transcriptomics-RNA-Seq analysis of primary tumors identified 2697 downregulated and 3534 upregulated genes, indicating broad transcriptional reprogramming following sarcophine treatments. These findings demonstrate coordinated suppression of multi-oncogenic pathways and validate the therapeutic potential of sarcophine to control mCRPC. Full article

Global freshwater salinization endangers aquatic species, yet its impacts on crustaceans remain poorly understood. This study investigated the hepatopancreatic response of Eriocheir sinensis to carbonate alkalinity stress (0, 4.375, 8.75, 17.5, and 35 mmol/L) over 24, 48, and 96 h, integrating histology, ultrastructure, gene expression (RT-qPCR), and non-specific immune enzyme assays. Histopathological and ultrastructural analyses revealed concentration- and time-dependent damage, including vacuolization, hepatic tubule disintegration, nuclear condensation, mitochondrial reduction, and loss of cellular integrity. Molecular analysis demonstrated upregulation of genes associated with the TLR2-MyD88-NF-κB pathway and inflammatory genes (LITAF, IL-16), alongside increased HSP70 expression, confirming severe inflammation and cellular stress. Furthermore, apoptosis was induced via upregulated Bax and Caspase-3, downregulated Bcl-2, and DNA fragmentation. Non-specific immune responses in the hepatopancreas exhibited dynamic changes: acid phosphatase (ACP) was initially activated at low alkalinity but inhibited at high concentrations, while alkaline phosphatase (AKP) activity increased at 96 h. Notably, the hepatopancreas proved more sensitive to this stress than the hemolymph. Collectively, carbonate alkalinity causes multidimensional hepatopancreatic injury in E. sinensis through structural disruption, inflammation mediated by TLR2-MyD88-NF-κB signaling pathway-related genes, apoptosis induction, and immune enzyme dysregulation, posing a significant threat to crab health in salinized waters. Full article

To further investigate altitude-associated variations in gut microbiota and serum metabolites of plateau zokors (Eospalax baileyi) and elucidate their adaptive mechanisms to high-altitude environments, we performed fecal metagenomic sequencing and serum metabolomic profiling (Q200 platform) on individuals from high (3700 m, n = 6) and low (2700 m, n = 6) elevations, followed by integrated analysis of microbial and metabolomic datasets. Results indicated that in high-altitude plateau zokors, the relative abundance of Firmicutes decreased, while that of Bacteroidota increased. The dominant genera within this group were identified as Bacteroides and unclassified members of the Lachnospiraceae family. Moreover, the abundances of Bacteroides and unclassified members of the Muribaculaceae family increased with elevation. At the species level, seven fully annotated differentially abundant taxa were identified: Candidatus Amulumruptor caecigallinarius, Schaedlerella arabinosiphila, Muribaculum gordoncarteri, Heminiphilus faecis, Prevotellamassilia timonensis, Staphylococcus aureus, and Bacteroides graminisolvens. KEGG enrichment analysis indicated significant upregulation (p < 0.05) of energy supply pathways, such as oxidative phosphorylation, and antioxidant-related pathways, including β-alanine and lysine metabolism, in the high-altitude group. Conversely, cysteine and methionine metabolism pathways were markedly downregulated (p < 0.05). Serum levels of ursodeoxycholic acid and tauroursodeoxycholic acid (TUDCA) were significantly elevated (p < 0.05), while deoxycholic acid (DCA) levels decreased (p < 0.05). In conclusion, the composition and function of gut microbiota, along with serum metabolite profiles, differ significantly (p < 0.05) between plateau zokors from different altitudes. Through synergistic interactions between gut microbiota and host metabolites, plateau zokors develop adaptive mechanisms that integrate energy metabolism, oxidative stress response, intestinal barrier integrity, and mucosal immunity. This ultimately facilitates their acclimatization to high-altitude extreme environments characterized by hypoxia and low temperatures. Full article

Background: The continued evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) created ongoing challenges for molecular diagnostics and variant surveillance. Assays capable of maintaining diagnostic sensitivity across emerging variants while providing variant-related information remain essential for clinical and public health applications. This study evaluated the performance of the GXT96 X3 extraction kit in combination with the FluoroType^® SARS-CoV-2 varID Q version 1.0 assay (Hain LifeScience SA (Pty) Ltd., South Africa) for the detection, semi-quantitative assessment, and variant characterization of SARS-CoV-2 under laboratory conditions. Methods: A total of 220 samples were evaluated, including residual nasopharyngeal clinical specimens (n = 183), reference materials, and cultured SARS-CoV-2 virus dilutions. Residual specimens collected during multiple COVID-19 waves in South Africa (wild-type, Beta, Delta, and Omicron) were compared against standard-of-care (SOC) molecular assays used for routine diagnosis. RNA extraction was performed using the automated GXT96 X3 platform, followed by amplification on the FluoroCycler^® XT using the FluoroType^® SARS-CoV-2 varID Q assay targeting RdRp and N genes, with additional spike gene mutation detection for variant detection. Diagnostic accuracy, agreement (Cohen’s kappa), precision, linearity, and limit of detection (LoD) were assessed. Results: The assay demonstrated a sensitivity of 98.4% (95% CI: 94.2–99.8) and specificity of 100% (95% CI: 95.9–100.0) compared with SOC assays, with an overall agreement of κ = 0.981. Precision analysis showed acceptable reproducibility with a standard deviation of ≤1.49 and a coefficient of variation of ≤3.83%. Regression analysis demonstrated linearity across the dilution series (R² = 0.9882 for RdRp and 0.994 for N genes). The LoD was ≤100 copies/mL for the RdRp gene and 250 copies/mL for the N gene. Variant-associated spike mutations corresponded broadly with epidemiological wave patterns observed in South Africa. Conclusions: Under the evaluated laboratory conditions, the GXT96 X3 extraction platform combined with the FluoroType^® SARS-CoV-2 varID Q assay demonstrated high diagnostic accuracy and reproducibility for SARS-CoV-2 detection across a range of viral loads with additional spike gene mutation detection as an adjunct feature. Full article

The understorey vegetation of mature Quercus robur L. mixed forests (MF) was compared for richness and composition with that of natural forest gaps (FG) and of 30-year-old artificial Q. robur plantations (AF) in Mediterranean Italy. Richness was similar in AF and MF and was almost double in FG, due to the arrival of species from non-forest habitats, among which 14% were aliens. In these specific conditions, natural gap dynamics did not support forest recruitment, while AF hosted typical nemoral species, demonstrating that afforestation may be successful for nature restoration, provided that connectivity with natural systems is maintained for the supply of forest species propagules. Full article

The constitutive analysis model and hot processing map of the ZM6 alloy across various deformation conditions were investigated during hot compression experiments. True stress-strain curves within 300–450 °C and 0.0001–0.1 s⁻¹ were obtained from compression tests on a Gleeble-1500 platform. The results showed that higher strain rates (e.g., 0.1 s⁻¹) induced pronounced work hardening, whereas high temperatures (300–400 °C) combined with low strain rates (10⁻⁴ s⁻¹) promoted conditions conducive to dynamic recrystallization (DRX), leading to a softening tendency of steady-state flow stress. Additionally, a modified strain-compensated constitutive model was built for flow stress prediction. Material constants were plotted as fifth-order polynomial functions of strain (0.025–0.80) for precise stress predictions. The derived activation energy (Q = 182.38 kJ/mol) falls within the typical range for Mg-RE alloys. Leave-one-temperature-out cross-validation showed average AARE values of 7.2–9.8%, demonstrating the model’s interpolation capability and its sensitivity to extrapolation. Cross-validation within the training dataset showed reasonable consistency between experimental and predicted stresses (R > 0.997, AARE < 4.35%). Using the dynamic materials model, hot processing maps identified safe deformation zones and instability zones of the ZM6 alloy. Flow instability was observed at strain rates >0.01 s⁻¹, particularly at low temperatures (300–350 °C). Optimal processing windows appeared in high-energy dissipation (η > 30%) regions, e.g., 400–450 °C/10⁻⁴–10⁻³ s⁻¹. Optical microscopy confirmed that at high temperatures (≥400 °C) and low strain rates (≤0.001 s⁻¹), a uniform, fine-grained, fully recrystallized structure can be obtained, whereas low temperatures (350 °C) and high strain rates (0.1 s⁻¹) produce coarse elongated grains with limited DRX, consistent with the instability regime predicted by the processing maps. Under intermediate conditions (e.g., 400 °C, 0.01 s⁻¹), a bimodal grain distribution indicates incomplete recrystallization. Although EBSD analysis was not performed in this study, the optical microstructures directly validate the predicted safe and unstable windows. Together, all these findings provide preliminary model-based guidance for optimizing hot working parameters to balance microstructural stability and processing efficiency. Full article

Background: Eggshell membrane peptides (ESMPs) are natural bioactive compounds with reported chondroprotective properties. However, their regulatory effects on canine chondrocytes remain unclear. This study investigated ESMP in an interleukin-1β (IL-1β)-induced inflammatory model of canine chondrocytes. Methods: Chondrocytes were assigned to control (Cont), IL-1β, and ESMP + IL-1β groups. Cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay. NF-κB p65 nuclear translocation was evaluated by immunofluorescence staining. Real-time quantitative PCR (RT-qPCR) and Western blotting (WB) were used to measure mRNA and protein expression levels, respectively. Results: ESMP inhibited IL-1β-induced NF-κB p65 nuclear translocation and reduced the IL-1β-induced increases in interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and matrix metalloproteinase-13 (MMP-13) at both mRNA and protein levels. ESMP also decreased IL-6, nitric oxide (NO), and prostaglandin E₂ (PGE₂) levels in culture supernatants. ESMP reversed the IL-1β-induced reduction in type II collagen α1 chain (COL2A1) and aggrecan (ACAN) expression at both transcriptional and protein levels. Conclusions: ESMP attenuates IL-1β-induced inflammatory responses and extracellular matrix degradation in canine chondrocytes, potentially associated with suppression of NF-κB p65 nuclear translocation. This supports its potential application in promoting joint health in dogs. Full article

Background/Objectives: The aim of this study was to investigate primary colonizers adhering to the oral biofilm on five adhesive restorative materials. Methods: For each material (Admira Fusion, Clearfil AP-X, Durafill VS, Filtek Supreme XTE, and Venus Diamond), sixteen test specimens were prepared according to a standardized protocol. For pellicle formation, the specimens were incubated for two hours at 37 °C with sterile-filtered inactivated human saliva. The bacteria (Streptococcus oralis (S. oralis), Streptococcus gordonii (S. gordonii), Streptococcus sanguinis (S. sanguinis), Streptococcus mitis (S. mitis), and Actinomyces naeslundii (A. naeslundii)) were cultivated and suspended. A bacteria mix was prepared from the suspensions. The specimens with pellicles were wetted with the bacterial mix and incubated at 37 °C for 8 h. The total genomic DNA of the adhered bacteria was isolated and subsequently quantified using SYBR Green qPCR. Results: For S. gordonii, S. oralis, and A. spp., no significant differences in the amount of adhered bacterial DNA were found between the different materials. S. mitis DNA concentration was significantly higher on Filtek Supreme XTE compared to the other materials. Significantly higher DNA concentrations of S. sanguinis could also be detected on Filtek Supreme XTE compared to Clearfil AP-AX and Durafill VS. Conclusions: The investigated restorative materials showed species-specific differences in bacterial adhesion, with Filtek Supreme XTE exhibiting higher adhesion of S. mitis and S. sanguinis, whereas no differences were observed for the other tested species. Full article

Secondary caching is increasingly important for RocksDB deployments on remote or disaggregated storage, where misses in the DRAM block cache incur costly backend reads. Although RocksDB introduced the experimental SecondaryCache interface to extend block caching beyond DRAM, practical public support remains limited and does not fully exploit heterogeneous local storage devices. We present CacheLink, a multi-device secondary caching framework for RocksDB that extends the SecondaryCache model with practical cache-management capabilities using CacheLib. CacheLink stores blocks evicted from the DRAM block cache on heterogeneous secondary devices such as HDDs, SSDs, and NVMe drives, thereby reducing access to slower backing storage. It further supports configurable random admission control and multiple eviction policies, including LRU, LRU2Q, and TinyLFU. Experimental results obtained using db_bench and YCSB show that CacheLink substantially improves throughput and reduces latency compared to baseline RocksDB without secondary caching. In the secondary cache size experiment, the best-performing configuration uses a 2 GB NVMe secondary cache with TinyLFU eviction and full admission, improving throughput by 2.5× and reducing average latency by 60.7% relative to the baseline. Full article

Background/Objectives: Sex estimation is a cornerstone of research and practice in bioarchaeology and forensic anthropology. However, morphological and metric methods are often hampered by population-specific variation, subjectivity in assessment, and taphonomy. This study compares morphological analysis and ancient DNA (aDNA)-based sex assessment in a 19th-century Portuguese sample to evaluate the accuracy of osteological (anthropological) criteria. Methods: This study analysed 37 skeletons from the Venerável Ordem Terceira da Nossa Senhora do Carmo burial grounds in Porto. Sex estimation was based on (1) the bioanthropological assessment of morphological traits of the os coxae and the skull (2) through aDNA analysis using a multi-marker approach, including real-time PCR (qPCR) targeting autosomal loci, the amelogenin locus, a Y-chromosomal INDEL, and Y-STRs. aDNA was extracted via a non-destructive protocol. Results: Whilst anthropological analysis was possible on all 37 individuals, estimation of sex through aDNA analysis was possible for 26 individuals. A 20% discordance rate was found between morphological and aDNA results. Many individuals morphologically classified as “possible female” or “indeterminate” were genetically identified as male. Genetic analysis resolved most cases that biological anthropologists concluded were “indeterminate”. Conclusions: The high discordance in the Carmo sub-sample may indicate reduced skeletal sexual dimorphism, with males exhibiting skeletal traits typically associated with females, suggesting a sample-specific reduction in sexual dimorphism likely influenced by environmental, nutritional, and/or genetic stressors. A limitation of this study is its small sample size: only 26 of 37 individuals yielded usable genetic results, and only a portion of these individuals provided sufficient data for a direct comparison between morphological and genetic data. Nevertheless, these findings highlight the risk that applying generalised osteological standards relying solely on morphology can lead to systematic misclassification, emphasising the need for a critical, multidisciplinary approach to sex estimation. Full article

Although pathogenic Leptospira DNA has been detected in water and soil from different climatic regions, information from Mediterranean-climate agricultural systems remains limited. This study characterized the environmental detection of pathogenic Leptospira DNA in water and soil samples from irrigated agroecosystems of central Chile, evaluating spatial and seasonal variation and associations with selected physicochemical variables. A total of 605 samples were collected from eight agricultural sites during spring 2019, summer 2020, and winter 2021. Samples were analyzed by real-time PCR targeting lipL32. Overall, 29.1% of samples were PCR-positive, and pathogenic Leptospira DNA was detected in all sites and seasons. Soil samples showed higher positivity than water samples (34.5% vs. 21.4%), and positivity was higher in summer (41.7%) than in spring (22.7%) or winter (19.3%). Water temperature and turbidity were the only physicochemical variables that differed between positive and negative samples, whereas the binomial generalized linear mixed model (GLMM) showed that season and sample type were associated with PCR positivity after accounting for site-level clustering. These results show that pathogenic Leptospira DNA can be widely detected in irrigated agricultural systems from a Mediterranean-climate region, suggesting that soil, seasonality, irrigation practices, and other site-level characteristics should be considered in future studies on the environmental ecology of pathogenic Leptospira. Full article

Background/Objectives: Retinal optical coherence tomography (OCT) has emerged as a sensitive biomarker of neurodegeneration in multiple sclerosis (MS), yet eyes without overt optic neuritis (ON) are routinely pooled as “clinically unaffected” despite their heterogeneous histories. We evaluated whether never-ON eyes and fellow eyes after unilateral ON differ structurally and microvascularly using swept-source OCT (SS-OCT) and OCT angiography (OCTA). Methods: In this cross-sectional, single-center study, 126 clinically unaffected MS eyes—96 never-ON eyes and 30 fellow eyes after unilateral ON—were compared with 118 healthy control eyes. SS-OCT quantified ganglion cell–inner plexiform layer (GCIPL), peripapillary retinal nerve fiber layer (pRNFL), and macular RNFL (mRNFL) thickness, while OCTA measured superficial vascular plexus (SVP) vessel density. Between-group differences were assessed using generalized estimating equations with participant-level clustering, empirical (sandwich) standard errors, adjustment for age and sex, and false discovery rate correction. Results: Despite preserved visual acuity, both never-ON and fellow eyes showed structural retinal thinning relative to controls. GCIPL thickness followed a stepwise gradient—66.14 ± 4.31, 62.08 ± 7.03, and 58.03 ± 7.71 µm in controls, never-ON eyes, and fellow eyes, respectively (FDR-adjusted q = 0.020 for fellow vs. never-ON eyes)—and pRNFL and mRNFL showed a similar overall pattern. After false discovery rate correction, OCTA parameters did not differ significantly between groups. Conclusions: Clinically unaffected eyes in MS are not structurally normal, and fellow eyes after unilateral ON carry a greater burden of silent retinal damage than never-ON eyes. These two phenotypes should be analyzed separately in MS imaging research. Structural OCT measures, particularly GCIPL thickness, appear more sensitive than microvascular indices for detecting subclinical retinal involvement. Full article

Introduction: Marine heatwaves are increasing in frequency and intensity, posing major challenges for fishes inhabiting shallow coastal ecosystems. Short-term exposure to extreme warming can alter metabolic performance and thermal tolerance, with potential consequences for species persistence and school composition in thermally variable habitats. Understanding the capacity of coastal fishes to withstand acute warming events is therefore essential for predicting ecological responses to climate change. Objective: We aimed to determine the effects of simulated marine heatwaves on thermal tolerance and metabolic performance in juvenile grey mullets, Chelon labrosus and Chelon aurata, two abundant sympatric species inhabiting the Ria Formosa lagoon (southern Portugal). Methodology: Juvenile mullets acclimated at 17 °C were exposed to simulated heatwave treatments of 23, 27, or 33 °C and sampled either at peak temperature or after 48 h and 1-week recovery at 17 °C. Critical thermal maximum (CTmax, using a 1 °C/min thermal ramp), static oxygen consumption (MO2), and intermittent respirometry parameters were measured. Standard metabolic rate (SMR), maximum metabolic rate (MMR), and aerobic scope (AS) were derived from intermittent respirometry. A complementary temperature-ramp (>3 h at each temperature step 17, 23, 27 and 33 °C) was performed to evaluate routine metabolic rate and estimate Q10 values across increasing temperatures. Additional plasma and tissue analyses are being conducted to assess energetic substrate mobilization and cellular responses to thermal and oxidative stress. Results: CTmax increased significantly with warming in both treatment modes, demonstrating rapid heat hardening in juvenile mullets. Fish exposed to 27 and 33 °C exhibited higher CTmax than control fish, and this elevated tolerance persisted after recovery. Chelon labrosus showed slightly higher CTmax values than C. aurata. Oxygen consumption increased with temperature, with the strongest responses occurring at 33 °C. SMR increased markedly with warming, particularly in heatwave-exposed fish, while MMR increased mainly at the highest temperature treatment. In contrast, AS showed no clear thermal optimum or decline across treatments. Routine metabolic rate increased non-linearly with temperature in the complementary ramp experiment, with a mean Q10 of 2.28, confirming strong thermal dependence of metabolism. Conclusions: Juvenile mullets possess substantial short-term thermal plasticity and can rapidly increase heat tolerance during marine heatwaves but this enhanced tolerance is accompanied by elevated metabolic costs under extreme warming, indicating potential energetic trade-offs near upper thermal limits. Differential physiological responses between species may influence school composition and ecological performance across thermal landscapes. Ongoing plasma and tissue analyses will further clarify the energetic and cellular mechanisms underlying thermal and oxidative stress resilience in coastal fishes. Full article