Search Results (658)

Objective: It is commonly believed in the public health community that the mRNA vaccines from Pfizer and Moderna were broadly similar in preventing COVID-19 mortality, with perhaps a small advantage for Moderna. We revisit this question, using a research design that controls for healthy-vaccinee bias. Methods: We studied deaths among persons aged 15 years or greater from January 2021 through December 2022 in Indiana, linked to vaccination records, using an outcome measure, namely COVID-19 Excess Mortality Percentage (CEMP), which uses non-COVID natural mortality to control for HVB. Results: We find both large healthy-vaccinee bias for both vaccines, and larger bias for Pfizervaccinees: Pfizer recipients were healthier and thus would have faced lower COVID-19 mortality without vaccination. For ages 60+, we find almost 50% higher COVID-19 remaining mortality risk (RMR) for Pfizer vaccinees receiving primary vaccination over a “core period” from 2Q2021 through 1Q2022: the average Pfizer/Moderna RMR ratio was 1.49. For ages 15–59, this ratio was 1.25, but was not statistically significant. For booster dose for ages 60+, the average Pfizer/Moderna RMR ratio was 1.34. For 2Q2022 through 4Q2022, after the major Omicron wave, we find much lower vaccine effectiveness and no significant Pfizer–Moderna difference. Conclusions: During the period with both good vaccine availability and high COVID-19 mortality (2Q-2021–1Q2022), the Moderna vaccine was associated with lower COVID-19 mortality, relative to the Pfizer vaccine. A possible explanation is the higher Moderna dose (100 μg vs. 30 for Pfizer). After that period, a high percentage of the population had natural immunity from prior COVID-19 infection; we find only small mortality gains from vaccination, with no significant Pfizer–Moderna difference. Policy Implications: Controlling for healthy-vaccinee bias, Moderna was associated with lower COVID-19 mortality, relative to Pfizer. This difference was not known in real-time due to the failure of the available studies to address healthy-vaccinee bias. Controlling for such bias is vital when making vaccination policy. Full article

Alphonse Mucha’s oeuvre is frequently categorized as a formalist paradigm of ornamentation within the Art Nouveau movement. This study, however, contends that Mucha’s signature Q Formula is far from a mere aesthetic redundancy or a utilitarian embellishment; rather, it manifests as Autonomous Ornament—an autonomous generative process and an inherent rhythm. Adopting a synchronic structural perspective that transcends linear historical causality, this research frames ornament as a dynamic relational medium connecting individual perception with collective evolution across geocultural boundaries. With Theosophy serving as a historical mediation and synthesizing Rudolf Arnheim’s psychology of visual perception with Gilles Deleuze’s philosophy of the ornamental refrain (ritornello), this paper traces the cross-cultural construction of the Q Formula—from Mucha’s Moravian Catholic roots to the avant-garde Theosophical milieu of Paris. Through a horizontal comparative analysis with contemporary fin-de-siècle artists—specifically contrasting Gustav Klimt’s ornamental dissolution and Aubrey Beardsley’s ironic fragmentation—the study underscores Mucha’s distinct integrative strategy. Furthermore, it demonstrates a profound structural homology in topological energy between the Q Formula and the Eastern Kālacakra Mandala. Within this pure and vital ornamental logic, the circular main body acts as a centripetal anchor referencing Emptiness (Śūnyatā), while the boundary-crossing media serve as a metaphor for centrifugal Dependent Origination (Pratītyasamutpāda) and worldly manifestation through clothed repetition. Such ornamental rhythms collapse the classical hierarchy between structure and surface. Amidst the pervasive anxiety of fin-de-siècle industrialization, these forms exerted a visual soothing function, providing deep emotional and psychological efficacy. Mucha’s ornamental schemata reveal how repetitive and structural rhythms successfully transmuted traditional religious paradigms into channels for the internal integration of the modern subject. The paper thus reaffirms ornament as a form of critical imagination and an independent artistic force, which not only reshapes the psychological homeostasis of the modern subject but also constructs a profound resonance between form and life within a cross-cultural context. Full article

Seed dormancy is a vital adaptive mechanism regulated by the DELAY OF GERMINATION (DOG) gene family. Here, we present the first systematic genome-wide identification and expression profiling of the DOG gene family (RsDOGs) in radish (Raphanus sativus). Comprehensive analysis of physicochemical properties, chromosomal distribution, and phylogeny revealed strong evolutionary conservation alongside functional divergence. Notably, several RsDOG proteins harbor natural fusions of the DOG1 domain with a bZIP domain, suggesting potential transcription factor activity and novel regulatory functions. Promoter analysis identified abundant ABA-responsive and abiotic stress-responsive cis-acting elements. Quantitative real-time PCR (qRT-PCR) profiling across contrasting cultivars—dormant variety Rs275 and non-dormant variety Rs100—at 4 h and 28 h post-imbibition revealed distinct differential expression patterns. In particular, TRs0x5c022182.1 exhibited high expression levels in dormant seeds, suggesting its potential involvement in seed dormancy regulation in radish. This study provides key insights into the structural evolution and expression dynamics of RsDOG genes, laying a solid foundation for molecular breeding aimed at optimizing seed germination traits. Full article

Artemisia scoparia is a classic medicinal and edible plant with great exploitation potential in anti-inflammatory research. This study aimed to explore its anti-ulcerative colitis bioactive constituents and corresponding mechanisms of action. A total of 15 flavonoids and coumarins were identified by macroporous resin purification combined with ultra-performance liquid chromatography coupled to UPLC-Q-Exactive Orbitrap MS/MS. Seven key bioactive compounds (eupatilin, cirsimaritin, quercetin, scoparone, 7-hydroxycoumarin, scopoletin and esculetin) and six core targets (TNF, AKT1, SRC, EGFR, HSP90AA1 and ESR1) were further screened via network pharmacology. Subsequent molecular docking analysis verified that flavonoids exhibited markedly stronger binding affinity to target proteins than coumarins. In vitro cellular experiments revealed that eupatilin exhibited the strongest anti-inflammatory activity among all key components. In vivo animal assays further validated that eupatilin alleviated colonic injury and markedly reduced the levels of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 in mice with DSS-induced ulcerative colitis. This work provides vital experimental evidence for the development and utilization of anti-ulcerative colitis ingredients from A. scoparia, and validates its promising health-related application value in the food industry. Full article

Rotaviruses (RVs) are important pathogens which induce gastroenteritis in different kinds of animals, including mammals and birds. Rotaviruses are divided into nine species (RVA-RVD and RVF-RVJ), and RVA-RVC and RVH can infect both humans and pigs. It is vital to understand the genetic diversity and evolution of porcine rotavirus (PoRV) for effective prevention and control of this disease. In this study, 5320 intestinal tissue samples and fecal swabs were collected from different pig farms in Guangxi Province, southern China, from 2022 to 2025. These samples were tested for PoRV species A (PoRVA), PoRVB, PoRVC, and PoRVH using the multiplex RT-qPCR. The positive samples of PoRVA were further selected to amplify and analyze the VP4, VP6, and VP7 gene sequences. The phylogenetic trees were constructed based on the PoRVA VP4, VP6, and VP7 gene sequences. Bayesian time-dynamic analysis and recombination analysis were performed for the PoRVA VP4 gene. The results indicated that the PoRVA, PoRVB, PoRVC, and PoRVH positivity rates were 16.92% (900/5320), 0.51% (27/5320), 12.71% (676/5320), and 6.22% (331/5320), respectively. Fifty-two VP4, VP6, and VP7 gene sequences were obtained from the 52 selected PoRVA-positive clinical samples. The nucleotide and amino acid identity analysis of the obtained PoRVA VP4, VP6, and VP7 genes indicated that the genetic diversity of the VP4 gene was higher than that of the VP6 and VP7 genes. The phylogenetic trees based on the VP4, VP6, and VP7 genes revealed that the predominant strains of PoRVA in Guangxi Province were the G9P[13]I5 genotype. Bayesian analysis indicated that the population size of PoRVA kept steady with no significant expansion from its discovery in the 1970s to approximately 2016, then exhibited gradual growth. Sequence analysis of the PoRVA VP4 gene revealed substitutions and recombination in the PoRVA strains, and one strain was derived from recombination of a porcine-originating strain and a human-originating strain. This study provided useful information on the molecular characteristics and genetic diversity of PoRVA and supplied important clues for in-depth research on the cross-species transmission of PoRVA. Full article

Plant-based platforms remain vital for producing anticancer vinca alkaloids. However, one major challenge in plant-based metabolic engineering is that target metabolite yields are often hindered by the presence of multiple competing pathway branches that disperse metabolic flux. To overcome these bottlenecks, we first implemented a competitive branch-point diversion strategy by co-overexpressing geissoschizine synthase and catharanthine synthase under an inducible promoter in Catharanthus roseus hairy roots. This targeted intervention successfully shifted metabolic partitioning, driving a peak relative increase of 67% in catharanthine accumulation at the 96 h time point, while significantly reducing competing by-products such as ajmalicine, serpentine, and tabersonine. Targeted qPCR analysis revealed a transient upregulation of core monoterpene indole alkaloid biosynthetic genes and a coordinated suppression of some competing branch genes. Despite this effective downstream diversion, overall yield enhancement was constrained by tight regulation, notably the downregulation of BIS transcriptional activators, which restricts the supply of upstream terpenoid precursors. Furthermore, untargeted metabolomic profiling highlighted a widespread metabolic shifts featuring the significant enrichment of phenylalanine-derived secondary pathways. Ultimately, this study demonstrates the efficacy of competitive branch-point diversion for target alkaloid enrichment, while highlighting the complex regulatory dynamics and broad metabolic alterations within the C. roseus network. Full article

Hydrogen sulfide (H₂S) is a vital gasotransmitter essential for maintaining redox homeostasis and modulating inflammatory responses. Herein, we examined a collection of Drosophila melanogaster knockout (KO) lines generated in our laboratory, lacking key genes involved in transsulfuration (cbs, cse) and sulfide metabolism (tst1), to elucidate the role of this adaptive system in immunity. Genetic ablation of these pathways results in profound H₂S deficiency and hyperhomocysteinemia, leading to chronic oxidative stress. This leads to constitutive activation of major immune pathways—including IMD, Toll, and JAK-STAT—even in the absence of infection, a hallmark of chronic inflammation. Indeed, transcriptomic analysis and qRT-PCR studies revealed significant upregulation of these pathways in double (cbs; cse) and triple (cbs; cse; tst1) KO flies under control conditions. Following septic injury with Bacillus subtilis, double and triple KO flies exhibited increased expression of antimicrobial peptides (AMPs) and pattern recognition receptors compared to control and TST1-/- single KO lines. Notably, double and triple KO lines showed more prolonged immune activation. The triple KO flies exhibited the worst survival rate following septic injury. These results demonstrate that H₂S deficiency causes chronic inflammation through the sustained activation of immune pathways, highlighting sulfur metabolism as a crucial regulator of homeostasis and innate immunity in Drosophila. Full article

Background: As a major regulator, methyltransferase-like 3 (METTL3) catalyzes N6-methyladenosine (m⁶A) modification in mRNA. The m⁶A modifications mediated by METTL3 influence RNA splicing, nucleocytoplasmic distribution, stability, and other functions, thereby playing a vital and indispensable role in genetic regulatory network. Although several studies have shown its critical role in mRNA fate, the global pattern of mRNA methylation alteration driven by METTL3 remain unclear. Methods: Here, a HEK293T cell line with METTL3 depletion was constructed, and RNA sequencing (RNA-seq) and methylated RNA Immunoprecipitation Sequencing (MeRIP-seq) were implemented. Additionally, quantitative Reverse Transcription PCR (qRT-PCR) technology was used to confirm some of the differentially expressed genes. Result: The mRNA methylation alteration landscape was clarified and the regions altered by m⁶A modification due to METTL3 deletion that was annotated and characterized, with 5763 hypomethylated/269 hypermethylated genes after METTL3 silence. Several methylation-related innate anti-infection immune genes, including MYD88, RIG-1, CYLD and IRF9, were exposed through comprehensive analysis to MeRIP-seq and RNA-seq data, and these genes were principally enriched in pathogen infection and innate immune response pathways such as Shigellosis, Yersinia infection, and the HIV-1 viral life cycle. Conclusion: Our study discovered that the METTL3 association with differentially expressed genes, suggested that METTL3 and the genes it regulates might serve as targets for defense against infection. Full article

The rapid development of airport economic zones has significantly reshaped regional land-use structures and industrial spatial organization. Taking the Nanjing Airport Economic Zone as the study area, this study integrates multi-source geospatial data, including land-use data, enterprise registration records, Points of Interest (POIs), transportation networks, nighttime light intensity, population, topography, and ecological-environmental variables for 2013, 2018, and 2023. Land-use transition matrices, spatial autocorrelation analysis, standard deviation ellipse analysis, Geodetector, and Multiscale Geographically Weighted Regression (MGWR) models were employed to examine land-use transition, industrial spatial restructuring, and their influencing factors from 2013 to 2023. The results show that: (1) Land-use change in the study area was mainly characterized by the decline of cropland, the expansion of impervious surfaces, and the shrinkage of water bodies. From 2013 to 2023, cropland decreased from 81.07 km² to 70.12 km², impervious surfaces increased from 10.98 km² to 25.65 km², and water bodies decreased from 5.50 km² to 1.79 km². The conversion from cropland to impervious surfaces was the dominant transition pathway, covering 14.67 km². (2) Industrial space exhibited significant spatial clustering, with a Moran’s I value of 0.9639 in 2023. The standard deviation ellipse results indicate that industrial space expanded during 2013–2018 and contracted during 2018–2023, suggesting a shift from extensive outward expansion to relative agglomeration around the core area and major transport corridors. (3) Nighttime light intensity and distance to major transport access points were important explanatory factors for industrial spatial distribution, with q-values of 0.396 and 0.310, respectively. The interaction between slope and metro accessibility showed the strongest explanatory power, with a q-value of 0.6967. The MGWR results further revealed the spatial heterogeneity of the effects of transportation, economic activity, population concentration, and ecological constraints. Overall, land-use transition and industrial spatial restructuring in the Nanjing Airport Economic Zone were jointly shaped by transportation accessibility, economic vitality, population agglomeration, and ecological constraints. These findings provide a reference for land-use optimization and industrial spatial governance in airport economic zones. Full article

This study evaluates the multi-year taxonomic diversity and functional structure of beetle assemblages (Coleoptera) within Sub Arini Park, a historic urban green space in Sibiu, Romania. Following a preliminary baseline and methodological calibration phase in 2023, systematic monitoring was conducted during the 2024 and 2025 seasonal cycles utilizing standardized pitfall trapping across diverse park zones. We explicitly tested two hypotheses: (H1) that long-standing historic park management preserves a resilient and functional insect community structure, and (H2) that local spatial heterogeneity and microhabitat variations significantly drive species distribution. A total of 14,843 individuals belonging to 39 species were analyzed. While total abundance exhibited a slight decrease from 2024 (N = 7112) to 2025 (N = 6551), true diversity metrics (Hill numbers) revealed a significant increase in raw species richness (q = 0) from 30 to 39 species, alongside an enhanced equity of frequent species (Shannon diversity, q = 1, increased from 4.26 to 5.12). Functional guild analysis and multivariate PCA demonstrated a highly structured biocenotic distribution; specialist and hygrophilous species (e.g., Carabus variolosus Fabricius, 1787) were strictly constrained to high-humidity riparian corridors, whereas thermophilous generalists dominated open lawns under high anthropogenic stress. Our spatial analysis identified critical degradation within these heavily managed zones, specifically driven by intensive mowing, soil compaction, and organic debris removal. These findings confirm both hypotheses, revealing that the park operates as a heterogeneous mosaic of ecological refugia rather than a uniform habitat block. Crucially, this study provides a concrete, quantitative basis—derived from empirical thresholds of species richness, abundance shifts, and mapped microhabitat preferences—for implementing nature-based management strategies (such as establishing buffer zones with reduced mowing frequencies, limiting trampling, and retaining coarse woody debris) aimed at mitigating urban biodiversity loss and maintaining vital biological pest control services in Central–Eastern Europe. Full article

Reliable, high-quality rainfall data are vital for soil and water management, crop forecasting, and risk assessment. These applications are essential for food security, climate resilience, biodiversity monitoring, and rural livelihoods. Rainfall monitoring in Thailand is challenging due to the limited density of official stations and the inconsistent quality of data from multiple sources, compounded by calibration issues. This study introduces a comprehensive quality control (QC) approach tailored for the Thai context, presenting a systematic pipeline that clarifies the hierarchy and sequence of operations. The method uses rainfall data from 3075 stations of the Thai Meteorological Department (TMD) and the Thaiwater network. It includes basic QC for data completeness and advanced QC using a quality (Q) index to assess station reliability, diving the stations into five groups: poor (<50), moderate (50–80), acceptable (80–85), good (85–90), and excellent (>90). The results indicate that Thaiwater consistently achieved moderate to excellent Q index values, exceeding 70% annually, with values surpassing 90% in 2023. In contrast, the TMD maintained excellent quality, with values above 90% for all years. Out of over one million daily entries, 87% were verified as correct, though the Thaiwater data for 2024 showed only 70% accuracy. The QC procedures significantly improved data reliability, reducing the root mean square error for GSMaP and IMERG by 1.7% and 1.5%, respectively, and lowering the false alarm rate by approximately 0.001–0.002 without compromising heavy rainfall detection. A systematic QC framework is essential for ensuring high-quality datasets in rainfall applications. Full article

Brassica napus reproductive development and abiotic stress tolerance are critical for yield and quality, and characterizing key transcription factor families is vital for molecular breeding. Here, based on the B. napus cv. Darmor-bzh V5 reference genome, we systematically identified and analyzed the BnaS1fa gene family, uncovering 12 members. Their encoded proteins are mostly small, alkaline, stable, and hydrophilic, with a few having ultra-long structures. Phylogenetic analysis clustered them into three subfamilies; conserved motif and gene structure analyses revealed high overall family conservation with partial member differentiation. Promoter cis-acting element analysis showed enrichment in light, hormone, and stress-responsive elements. Chromosomal localization and intraspecific collinearity analyses indicated the family mainly derived from homologous fragment retention in A and C subgenomes. Transcriptome data demonstrated high BnaS1fa expression in late seed and silique development, with prominent heat stress responses. RT-qPCR, subcellular localization and transcriptional activity assays confirmed BnaS1fa9 and BnaS1fa10 as nuclear-localized transcription factors with heat stress-induced expression. This study elucidates BnaS1fa molecular characteristics and its potential roles in reproductive development and heat stress response, providing candidate genes for B. napus stress-resistant molecular breeding. Further functional validation of these key genes will facilitate the dissection of their precise regulatory mechanisms governing heat stress tolerance and reproductive growth, which can be ultimately applied to advance the genetic improvement of rapeseed stress resistance and yield performance. Full article

Coral mucus serves as a crucial defensive barrier for corals, where the mucus microbes play a vital role in the coral’s resistance to external stresses. However, detailed knowledge of the effect of UV radiation on coral mucus microbiome is limited, particularly regarding the UV resistance mechanisms of coral mucus microbes. This study investigates changes in the mucus microbial community and possible UV-resistant mechanisms of the mucus microbiota of Porites sp. and Favites sp. under UV stress using high-throughput sequencing, UV-resistant microbial isolation, and RT-qPCR analysis. Compared to the control, UV stress alters microbial community structure by reducing microbial diversity, e.g., the relative abundance of Aquibacter, Agaribacter, and Oleibacter in coral Porites sp., and the Roseobacter clade CHAB_I_5 lineage, Roseivirga, and Nautella in coral Favites sp. increase under UV stress. Meanwhile, it is indicated that the Favites sp. mucus microbiome is much more sensitive than the Porites sp. mucus microbiome. A total of 428 microbial strains belonging to 5 phyla, 7 classes, 15 orders, 23 families, and 47 genera were isolated from these two coral mucus species, with Ruegeria and Rossellomorea as the most abundant cultured taxa. Pseudoalteromonas galatheae strain P12 and Limimaricola pyoseonensis strains P2 and P9 have been proven to exhibit higher UV resistance by enhanced expression of tyr, sod, gogat, and uvrC genes, indicating that the UV resistance of coral mucus bacteria involves complex molecular processes, including upregulation of antioxidant enzyme expression and enhancement of melanin and glutamic acid biosynthesis. These findings enhance our understanding of coral mucus microbial ecological functions, particularly highlighting the coral mucus microbial UV resistance strategy. Full article

Despite the recognized role of oxidative stress (OS) in sperm function, limited data exist on Nuclear factor-E2-related factor 2 (Nrf2) pathway modulation in relation to reliable oxidative damage markers in human semen. In this study, 79 semen samples were collected from men undergoing semen analysis and grouped as varicocele (V, no. 22), urogenital infections (UI, no. 23), unknown fertility status without pathologies (UFS, no. 15), and fertile controls (F, no. 19). After semen analysis, ELISA were used to quantify F₂-Isoprostane (F₂-IsoPs) level, a marker of lipid peroxidation, and Nrf2 in seminal plasma and spermatozoa. The Nrf2 pathway (Keap1, Nrf2, Bach1, HO-1) was assessed in spermatozoa by qRT-PCR. Seminal plasma and sperm Nrf2 positively correlated with F₂-IsoPs (p < 0.001) and negatively with sperm vitality. Sperm Nrf2 also inversely correlated with progressive motility (p < 0.05). Seminal F₂-IsoP levels were lower in F than in the other groups. Sperm Nrf2 was significantly lower in F versus V and UI (p < 0.001) and UFS (p < 0.05), while seminal plasma Nrf2 levels did not differ among groups. qRT-PCR suggested Nrf2 pathway activation mainly in V and UI, consistent with increased OS. Elevated F₂-IsoPs, a marker of poor sperm quality, and sperm Nrf2 could suggest OS-driven Nrf2 activation, providing complementary biomarkers of oxidative status in male reproductive health. Full article

The widespread use of pesticides in agriculture has increased their levels in water bodies, threatening aquatic ecosystems. Among these, glyphosate and chlorpyrifos are widely used in Mexico and can cause toxic effects even at low doses. In aquatic organisms, early exposure to these pollutants can disrupt vital processes, such as sex determination, through oxidative stress. This study assessed the effects of exposure to environmental concentrations of glyphosate (100 μg/L), chlorpyrifos (1.5 μg/L), and their combination on zebrafish (Danio rerio) from early stages to 90 days post fertilisation (dpf). Survival was measured using Kaplan–Meier curves; lipid peroxidation was assessed by malondialdehyde (MDA); sex-related gene expression was measured by qPCR of selected genes at 30 dpf; and gonadal development was assessed by histology at 65 dpf. The results showed increased MDA levels in exposed fish. Glyphosate caused early toxicity and a higher proportion of undifferentiated fish, implying delayed sex determination. Chlorpyrifos induced oxidative stress and affected amh gene expression linked to masculinisation. Combined exposure reduced survival and altered gene expression and gonadal development. Exposure shifted the sex ratio toward males, suggesting that pesticide-induced oxidative stress may alter the expression of sex determination genes during early development. Full article