Search Results (60)

Polygala tenuifolia Willd., a widely used traditional Chinese medicine, has the function of coordinating heart and kidney and eliminating swelling. However, its renal-protective efficacy and possible material basis remain unknown. The aim of the study was to investigate the renal-protective effect of Polygala tenuifolia Willd. and identify the potential active substance and molecular mechanism. A gentamicin-induced kidney injury model was established to investigate efficacy. Secondly, potential active substances and molecular mechanisms were studied through integrated gut microbiota–drug interaction analysis and network pharmacology at a cellular level. Finally, 16S rRNA sequencing and untargeted metabolomics were used to elucidate the gut microbiota composition and metabolic profile change. Polygala tenuifolia Willd. extracts (PWE), with tenuifoliside A (TFSA) as the key compound, significantly reversed gentamicin-induced acute kidney injury in mice. The gut microbiota-derived carboxylesterase metabolized TFSA into four characteristic metabolites (M1–M4). Notably, both TFSA and M4 were detected in kidney and exerted protective effects via inhibiting TLR4–NF-κB pathway. Furthermore, metabolic pathways and gut microbiota composition change were identified. PWE treatment significantly increased the abundance of beneficial bacteria such as Akkermansia and Blautia, while reducing the abundance of harmful bacteria such as Oscillospira. Subsequently, PWE can reverse amino acid metabolic abnormalities by regulating the biosynthesis of phenylalanine, tyrosine, and tryptophan and ameliorating tryptophan metabolism disorder. This study was the first to verify the renal-protective effect of PWE and identify the effective substance basis (TFSA) and the molecular mechanism, providing a scientific foundation for the development of kidney drug treatment strategies targeting the intestinal flora. Full article

Background: Heart rate variability (HRV) is a well-established marker of autonomic nervous system (ANS) activity. It is also an important tool for investigating cardiovascular and neurological health. Changes in HRV have been associated with epilepsy and sudden unexpected death in epilepsy (SUDEP), conditions in which autonomic dysregulation is believed to play a significant role. HRV is traditionally measured using electrocardiography (ECG) under standardized laboratory conditions. Recently, however, wearable devices such as the Empatica E4 wristband have emerged as promising tools for continuous, noninvasive HRV monitoring in real-life, ambulatory, and clinical settings where laboratory infrastructure may be lacking. Methods: We evaluated the validity and clinical utility of the Empatica E4 wristband in two cohorts. In the first cohort of healthy controls (n = 29), we compared HRV measures obtained with the E4 against those obtained with a gold-standard laboratory ECG device under seated rest and metronomic breathing conditions. In persons with epilepsy (PWE, n = 42), we assessed HRV across wake and sleep states, as well as during exposure to sodium channel blockers. This was done to determine whether the device could detect physiologically and clinically meaningful changes in autonomic nervous system (ANS) function. Results: In healthy participants, the Empatica E4 provided heart rate (HR), root mean square of successive R-R intervals (RMSSD), and standard deviation of all interbeat intervals (SDNN) values that were strongly correlated with laboratory measurements. Both devices detected the expected increase in RMSSD during metronomic breathing; however, the E4 consistently reported higher absolute values than the ECG. In patients with epilepsy (PWE), the E4 reliably captured parasympathetic activation during sleep and detected a significant reduction in heart rate variability (HRV) in patients taking sodium channel blockers, demonstrating its sensitivity to clinically relevant autonomic changes. Conclusions: The Empatica E4 wristband is valid for measuring HRV in research and clinical contexts. It can detect modulations of ANS activity that are physiologically meaningful. While HRV metrics were robust, other signals, such as electrodermal activity and temperature, were less reliable. These results highlight the potential of wearable devices as practical alternatives to laboratory-based autonomic testing, especially in emergency and resource-limited settings, and emphasize their importance in epilepsy care risk assessment. Full article

Soil surfactants are essential tools for enhancing irrigation water efficiency and improving the quality and functionality of amenity turfgrass. They play a crucial role in sports turf management by reducing soil water repellency, which helps prevent dry spots, ensures even moisture distribution, and supports water conservation efforts. Most research on soil surfactants and amenity turfgrasses focuses on their effects on soil moisture, infiltration, and addressing localized dry spots during drought conditions, with limited studies on their impact under wet or saturated conditions. This study aimed to evaluate the impact of soil surfactants on the quality and health of turfgrass under wet conditions. Field studies were conducted over a span of five years, beginning in the USA in 2019 and continuing in Ireland from 2020 to 2023. The research in Ireland was conducted at three locations, each featuring different rootzones: a “push-up” green with loam soil, USGA-specification sand, and natural link sand. The site in the USA was a native loam soil. The study compared a commercial soil surfactant (ProWet Evolve; PWE) and a non-treated control (NT) in a randomized complete block design with four replications, with sequential applications starting in June and continuing until mid-September each year. The rootzone volumetric water content (VWC%), turfgrass quality, and normalized difference vegetation index (NDVI) were measured bi-weekly. Environmental conditions, with above-average precipitation each year, significantly influenced results. Although there were no significant or consistent differences in VWC% between the soil surfactant and NT-treated plots, turfgrass quality was significantly enhanced in the soil surfactant-treated plots and supported by higher NDVI values. Even in prolonged wet conditions with high VWC%, improved turfgrass quality was consistently observed in soil surfactant-treated plots across multiple locations in both countries over the five-year study period. Full article

This study proposes the use of a low-symmetry hexagonal photonic crystal (LSHPC) incorporating Kerr-type nonlinearity to enhance self-collimation. The equifrequency contours (EFCs) of a C₂-symmetric LSHPC composed of nonlinear LiNbO₃ rods are analyzed as a function of the nonlinear refractive index. The self-collimation characteristics, transmission spectrum, group velocity dispersion (GVD), and third-order dispersion (TOD) are investigated using the Plane Wave Expansion (PWE) and Finite Difference Time Domain (FDTD) methods. The results demonstrate that increasing the nonlinear index leads to a significant flattening of the EFCs, which enhances self-collimation performance. Furthermore, symmetry-lowering perturbations improve beam confinement and enable all-angle self-collimation. These findings highlight the potential of Kerr-type nonlinear photonic crystals for integrated photonic circuits requiring precise control over light propagation. Full article

Introduction: Among U.S. adults, over 3 million report a history of epilepsy, accounting for nearly 1.2% of the population. Sleep deprivation is a well-known risk factor for increased likelihood, intensity, and length of seizures. However, the long-term impact of sleep deprivation on people with epilepsy is not well explored. The purpose of this study was to assess mortality risk among individuals with epilepsy based on sleep duration. Methods: Data from the 2008–2018 National Health Interview Survey (NHIS) were linked with mortality data from the National Death Index (NDI) for US adults aged 65 years and older. Survival curves showed the combined effect of sleep deprivation and epilepsy, using the Kaplan–Meier product-limit method to estimate the percent survival of the subject at each point in time. Results: For all-cause mortality, the unadjusted hazard ratio (HR) for sleep deprivation to no sleep deprivation among people with epilepsy (PWE) was HR = 1.92. The adjusted HR was elevated, HR = 1.94, among individuals who had epilepsy and sleep deprivation but close to 1.0 among individuals who had a history of sleep deprivation without epilepsy after adjusting for demographic and health variables. Conclusions: From a nationally representative sample, this first-of-its-kind study in the U.S. found that sleep deprivation and epilepsy combined have worse outcomes than sleep deprivation alone. Clinicians should screen and manage sleep disorders to improve their long-term prognosis of people with epilepsy. Full article

Air pollution is a major trigger for chronic respiratory and circulatory diseases. As a key component of air pollution, fine particulate matter (PM_2.5) exposure is largely determined by land use type and population density. However, simultaneous consideration of their spatiotemporal distribution is lacking in existing studies on PM_2.5 exposure. In this paper, we first assess the dynamic evolution of land use patterns in Gansu Province, China, from 2000 to 2020, using a land use transfer matrix and dynamic degree. Population-weighted exposure (PWE) to PM_2.5 is then evaluated for each land use type at provincial, city, and county levels, with seasonal variations analyzed. Spatial autocorrelation analysis is finally performed to explore the spatiotemporal evolution of PM_2.5 exposure, whereas standard deviation ellipses and gravity center migration models highlight spatial distribution characteristics and shifting trends. Experimental results showed that 2010 was a turning point for annual PM_2.5 exposure at the provincial level in Gansu Province, with an initial increase followed by a decrease. Construction land had the highest annual PM_2.5 exposure, whereas forest had the lowest exposure (except in 2005). Exposure levels showed a seasonal pattern: higher in winter and spring and lower in summer and autumn. At city and county levels, southern Gansu indicated a continuous decline in annual PM_2.5 exposure across all land use types since 2000. Exposure levels exhibited a strong spatial positive correlation, with a fluctuating spatial convergence. This study comprehensively analyzes the multi-scale differences and spatiotemporal evolution patterns of PM_2.5 exposure across various land use types, contributing to provide scientific evidence and decision-making support for mitigating air pollution and enhancing coordinated air pollution control at multi-scale administrative levels. Full article

This study offers promising insights into the anti-obesity potential of Pleurotus ferulae, an edible mushroom valued in Asian cuisine for its nutritional benefits. A hot water extract of P. ferulae (PWE) administered to high-fat diet-induced obese mice over an 8-week period significantly reduced their body weight gain and fat accumulation. PWE not only improved the body weight metrics but also positively influenced the serum lipid profile of obese mice by lowering their total cholesterol and low-density lipoprotein cholesterol levels. In vitro studies using 3T3-L1 adipocytes showed that PWE inhibited adipocyte differentiation and lipid accumulation by downregulating key adipogenic transcription factors, particularly PPARγ and C/EBPα, as well as related lipogenic genes involved in fat synthesis and storage, such as Fabp4, Fasn, and Scd1. Chemical analysis revealed that PWE is rich in polysaccharides, which have been associated with various health benefits, including anti-obesity, anti-diabetic, and anti-cancer properties. These findings suggest that the bioactive compounds in PWE may serve as functional food components that could potentially be applied for the prevention and management of obesity and other metabolic disorders. Full article

Air pollution is one of the main risk factors for human health. The aim of this study was to provide an Integrated Environmental and Health Impact Assessment (IEHIA) tool to estimate the impacts on both environment and human health in Pisa municipality (central Italy). For each pollutant considered (PM_2.5, PM₁₀, and NO₂), both Population-Weighted Exposure (PWE) and Attributable Deaths (ADs) were calculated considering the difference between the PWE and the latest air quality guidelines suggested by the World Health Organization. The PWEs were 16.1 µg/m³, 24.9 µg/m³, and 25.9 µg/m³ for PM_2.5, PM₁₀, and NO₂, respectively. The ADs from natural causes due to exposure to PM_2.5, PM₁₀, and NO₂ were 63, 29, and 51, respectively. The AD distribution was mainly concentrated in urban areas for particulate matter and in urban and suburban areas for NO₂. The results highlighted significantly higher levels of air pollution than the reference levels, with a percentage of ADs from natural causes of approximately 6% of the total mortality in Pisa. IEHIA offers support for environmental and health policies and territorial planning. The authors recommend the adoption of prevention measures aimed at mitigating air pollution in critical areas, with a consequent reduction in avoidable mortality. Full article

This paper proposes a unique configuration for an all-optical D Flip Flop (D-FF) utilizing a quasi-square ring resonator (RR) and T-Splitter, as well as NOT and OR logic gates within a 2-dimensional square lattice photonic crystal (PC) structure. The components realizing the all-optical D-FF comprise of optical waveguides in a 2D square lattice PC of 45 × 23 silicon (Si) rods in a silica (SiO₂) substrate. The utilization of these specific materials has facilitated the fabrication process of the design, diverging from alternative approaches that employ an air substrate, a method inherently unattainable in fabrication. The configuration underwent examination and simulation utilizing both plane-wave expansion (PWE) and finite-difference time-domain (FDTD) methodologies. The simulation outcomes demonstrate that the designed waveguides and RR effectively execute the operational principles of the D-FF by guiding light as intended. The suggested configuration holds promise as a logic block within all-optical arithmetic logic units (ALUs) designed for digital computing optical circuits. The design underwent optimization for operation within the C-band spectrum, particularly at 1550 nm. The outcomes reveal a distinct differentiation between logic states ‘1’ and ‘0’, enhancing robust decision-making on the receiver side and minimizing logic errors in the photonic decision circuit. The D-FF displays a contrast ratio (CR) of 4.77 dB, a stabilization time of 0.66 psec, and a footprint of 21 μm × 12 μm. Full article

Although Coronavirus disease 2019 (COVID-19) vaccinations are generally recommended for persons with epilepsy (PwE), a significant vaccination gap remains due to patient concerns over the risk of post-vaccination seizure aggravation (PVSA). In this single-centre, retrospective cohort study, we aimed to determine the early (7-day) and delayed (30-day) risk of PVSA, and to identify clinical predictors of PVSA among PwE. Adult epilepsy patients aged ≥18 years without a history of COVID-19 infection were recruited from a specialty epilepsy clinic in early 2022. Demographic, epilepsy characteristics, and vaccination data were extracted from a centralized electronic patient record. Seizure frequency before and after vaccination, vaccination-related adverse effects, and reasons for or against vaccination were obtained by a structured questionnaire. A total of 786 PwEs were included, of which 27.0% were drug-resistant. At the time of recruitment, 74.6% had at least 1 dose of the COVID-19 vaccine. Subjects with higher seizure frequency (p < 0.0005), on more anti-seizure medications (p = 0.004), or had drug-resistant epilepsy (p = 0.001) were less likely to be vaccinated. No significant increase in seizure frequency was observed in the early (7 days) and delayed phases (30 days) after vaccination in our cohort. On the contrary, there was an overall significant reduction in seizure frequency 30 days after vaccination (1.31 vs. 1.89, t = 3.436; p = 0.001). This difference was seen in both types of vaccine (BNT162b2 and CoronaVac) and drug-resistant epilepsy, but just missed significance for the second dose (1.13 vs. 1.87, t = 1.921; p = 0.055). Only 5.3% had PVSA after either dose of vaccine. Higher pre-vaccination seizure frequency of ≥1 per week (OR 3.01, 95% CI 1.05–8.62; p = 0.04) and drug-resistant status (OR 3.32, 95% CI 1.45–249 7.61; p = 0.005) were predictive of PVSA. Meanwhile, seizure freedom for 3 months before vaccination was independently associated with a lower risk of PVSA (OR 0.11, 95% CI 0.04–0.28; p < 0.0005). This may guide epilepsy treatment strategies to achieve better seizure control for at least 3 months prior to vaccination. As COVID-19 shifts to an endemic phase, this study provides important data demonstrating the overall safety of COVID-19 vaccinations among PwE. Identification of high-risk patients with subsequent individualized approaches in treatment and monitoring strategies may alleviate vaccination hesitancy among PwE. Full article

While significant strides have been made in comprehending the pathophysiology and treatment of epilepsy, further investigation is warranted to elucidate the factors impacting its development and transmission, particularly within familial contexts. This study sought to explore the prevalence and risk factors associated with epilepsy in the offspring of patients with epilepsy who were treated at a tertiary epilepsy center. Adult patients with confirmed epilepsy (PWE) receiving outpatient care were consecutively enrolled, starting from January 2021 to January 2023. Data were recorded for various variables, including age, gender, epilepsy pathophysiology, cognitive impairment, and family history of epilepsy. Descriptive statistics, various statistical tests, and multivariate logistic regression analyses were employed to analyze the data. A total of 1456 PWE were included. Among them, 463 patients (31.8%) had children. Twenty-five patients had offspring diagnosed with epilepsy, representing a prevalence of 5.4%. Analysis of the offspring with epilepsy revealed older ages, a higher proportion of parents with idiopathic epilepsy, and a greater prevalence of a positive family history of epilepsy. Multivariate logistic regression analysis demonstrated a significant association between a family history of epilepsy and increased epilepsy risk in offspring. Genetic syndrome-immanent predisposition, advanced age, and a family history of epilepsy were identified as significant risk factors for epilepsy in offspring by means of this mono-center study. Full article

Aiming to address the vibration noise problems on ships, we constructed a piezoelectric phononic crystal (PC) plate structure model, solved the governing equations of the structure using the partial differential equations module (PDE) in the finite element softwareCOMSOL6.1, and obtained the corresponding energy band structure, transmission curves, and vibration modal diagrams. The application of this method to probe the structural properties of two-dimensional piezoelectric PCs is described in detail. The calculation results obtained using this method were compared with the structures obtained using the traditional plane wave expansion method (PWE) and the finite element method (FE). The results were found to be in perfect agreement, which verified the feasibility of this method. To safely and effectively adjust the bandgap within a reasonable voltage range, this paper explored the order of magnitude of the plate thickness, the influence of the voltage on the bandgap, and the dependence between them. It was found that the smaller the order of magnitude of the plate thickness, the smaller the order of magnitude of the band in which the bandgap was located. The magnitude of the driving voltage that made the bandgap change became smaller accordingly. The new idea of attaching the PC plate to the conventional plate structure to achieve a vibration damping effect is also briefly introduced. Finally, the effects of lattice constant, plate width, and thickness on the bandgap were investigated. Full article

Objective: Cenobamate is approved by the European Medicine Agency for the treatment of adult patients with epilepsy (PWEs) with ongoing focal-onset seizures despite appropriate treatment with at least two established antiseizure medications. Pivotal trials and post-marketing real-world observational studies suggest high efficacy with unusually high seizure-free rates. The authors sought to investigate the plasma levels of cenobamate under steady-state conditions in seizure-free versus non-responding PWEs, and in PWEs who experienced adverse events versus those who did not. Methods: Blood samples were collected from adult PWEs who were treated with adjunct cenobamate under steady-state conditions. Daily doses, concomitant medications, efficacy, and tolerability were assessed. The plasma cenobamate levels of seizure-free versus non-responding PWEs and between PWEs with and those without clinical adverse events were compared. Results: Samples from 101 PWEs were included. Thirty-six PWEs were seizure-free and 65 were non-responders. In 31 PWEs, adverse events were apparent, whereas in the remaining 70, no tolerability issues were reported. A linear correlation was found between the daily doses (range: 100 mg–400 mg) and the plasma levels (3.8 mg/L–54.6 mg/L). Neither the daily doses nor the plasma levels differed significantly between the investigated subgroups. The main reason for this result was that the individual therapeutic ranges varied widely: seizure freedom and adverse effects were observed alongside low doses and plasma levels in some PWEs. Conversely, there were examples of PWEs who did not respond or who reported no tolerability issues at high doses or plasma levels. Conclusions: To evaluate the individual therapeutic range and to better understand the influence of other drugs in cases where concomitant medications are used, the therapeutic drug monitoring of cenobamate may be useful. A general therapeutic range cannot be defined. Full article

The most critical burden for People with Epilepsy (PwE) is represented by seizures, the unpredictability of which severely impacts quality of life. The design of real-time warning systems that can detect or even predict ictal events would enhance seizure management, leading to high benefits for PwE and their caregivers. In the past, various research works highlighted that seizure onset is anticipated by significant changes in autonomic cardiac control, which can be assessed through heart rate variability (HRV). This manuscript conducted a scoping review of the literature analyzing HRV-based methods for detecting or predicting ictal events. An initial search on the PubMed database returned 402 papers, 72 of which met the inclusion criteria and were included in the review. These results suggest that seizure detection is more accurate in neonatal and pediatric patients due to more significant autonomic modifications during the ictal transitions. In addition, conventional metrics are often incapable of capturing cardiac autonomic variations and should be replaced with more advanced methodologies, considering non-linear HRV features and machine learning tools for processing them. Finally, studies investigating wearable systems for heart monitoring denoted how HRV constitutes an efficient biomarker for seizure detection in patients presenting significant alterations in autonomic cardiac control during ictal events. Full article

In recent years, there has been a significant increase in research into silicon-based on-chip sensing. In this paper, a coupled cavity waveguide (CCW) based on a slab photonic crystal structure was designed for use as a label-free biosensor. The photonic crystal consisted of holes arranged in a triangular lattice. The incorporation of defects can be used to design sensor devices, which are highly sensitive to even slight alterations in the refractive index with a small quantity of analyte. The plane wave expansion method (PWE) was used to study the dispersion and profile of the CCW modes, and the finite difference time domain (FDTD) technique was used to study the transmission spectrum, quality factor, and sensitivity. We present an analysis of adiabatically coupling light into a coupled cavity waveguide. The results of the simulation indicated that a sensitivity of 203 nm/RIU and a quality factor of 13,360 could be achieved when the refractive indices were in the range of 1.33 to 1.55. Full article