Search Results (1,691)

The restoration of karst rocky desertification is reflected not only in the reduction in severely degraded areas but also in the stability of the restoration process and the potential risk of future reversal. Taking southeastern Yunnan, China, as the study area, this study constructed a six-period rocky desertification grade sequence for the years 2000, 2005, 2010, 2015, 2020, and 2024 using Landsat imagery, CLCD land-cover data, and DEM-derived slope constraints. Area change analysis, grade-transition matrices, Sen–MK trend analysis, coefficient of variation (CV), Markov–PLUS scenario simulation, scenario-sensitivity analysis, and PLUS driver contribution assessment were integrated into a process-oriented diagnostic framework to examine rocky desertification recovery from three dimensions: grade-structure adjustment, trend-variability stability, and potential future reversal risk. The results indicate that rocky desertification in southeastern Yunnan generally weakened from 2000 to 2024. The proportion of moderate-and-above rocky desertification decreased from 50.32% to 28.58%, while non-rocky desertification and potential rocky desertification expanded substantially. Grade transitions were dominated by gradual conversions among adjacent classes, with the most evident improvement occurring during 2010–2015, when the proportion of improvement transitions reached 44.16%. The trend-variability analysis indicated that while improvement dominated the study area overall, the northern, northwestern, central mountainous, and parts of the southwestern areas still exhibited relatively strong variability and localized deterioration risk. Hindcast validation showed relatively high map-level consistency between simulated and historical patterns, with an overall accuracy of 0.9354 and a Kappa coefficient of 0.9153. The three-scenario comparison further showed that the proportion of moderate-and-above rocky desertification varied from 28.07% to 29.41% in 2030 and from 26.85% to 29.06% in 2035 under different transition-probability assumptions. Specifically, the ecological restoration enhancement scenario reduced projected moderate-and-above rocky desertification, whereas the degradation pressure scenario increased it relative to the baseline scenario. These findings indicate that rocky desertification recovery in southeastern Yunnan is not a continuous or linear process, but is characterized by stage-specific adjustment, spatial differentiation, and local variability. Therefore, future rocky desertification control should focus not only on reducing high-severity areas, but also on maintaining restoration stability, identifying variability-sensitive transitional zones, and strengthening differentiated management in areas where terrain constraints, land-cover proximity, and historical variability jointly increase reversal risk. Full article

Saline–alkaline land serves as a potential arable land reserve for augmenting agricultural productivity and safeguarding food security. However, long-term monitoring of saline–alkaline land conversion remains challenging because of vegetation recovery, surface changes, hydrological modification, and agricultural phenology. Compared with CCDC and LandTrendr, the proposed MK-based framework detects conversion occurrence and timing while reducing dependence on dense observations, parameter tuning, and annual classification. This study examines the spatiotemporal dynamics of saline–alkaline land converted into paddies in Da’an City, utilizing Landsat time-series data (2007–2021) from the Google Earth Engine (GEE) platform. The analysis employed Mann–Kendall (MK) trend and mutation tests to monitor conversion processes and analyze spatiotemporal dynamics. Point-biserial correlation analysis was applied to evaluate the sensitivity of various remote sensing indices in detecting land conversion. The top fifteen indices, including the Land Surface Water Index (LSWI), Salinity Index 4 (SI4), and Salinity Index 5 (SI5), demonstrated strong correlations (|r| = 0.788–0.885) and significant pre- and post-conversion spectral differences (p < 0.01). Validation via confusion matrix confirmed that the June SI5 index attained the highest detection accuracy (overall accuracy: 94.15%; Kappa coefficient: 0.86), supporting the MK trend test’s efficacy in monitoring conversion processes. The MK mutation test achieved 80.36% temporal accuracy in determining conversion timing. The spatiotemporal analyses identified heterogeneity in saline–alkaline land conversion patterns. Spatially, large contiguous paddy fields dominated the eastern region, whereas fragmented conversion characterized the west, with minimal activity in the central zone. Temporally, the conversion area expanded rapidly before 2015 and then gradually declined, reaching a cumulative converted area of 276.29 km² by 2021. This study elucidates spatiotemporal conversion dynamics to guide sustainable land use. Full article

Background/Objectives: This study included first- to fifth-year dental students and aimed to compare dental anxiety levels using the Musa Kazım Dental Anxiety Scale (MK-DAS) and the Modified Dental Anxiety Scale (MDAS). Methods: A cross-sectional, web-based study was conducted at Altınbaş University Faculty of Dentistry (20–31 May 2024). A total of 200 students (40 per academic year) completed the 7-item MK-DAS (cut-off ≥ 17) and the 5-item MDAS (cut-off ≥ 15), and toothbrushing frequency was recorded. Between-year differences were assessed using Kruskal–Wallis tests with effect sizes (ε²). The association between academic year and anxiety scores was further evaluated using multivariable linear regression adjusted for age and gender. Concordance between scales was assessed using Spearman’s correlation. Statistical significance was set at α = 0.05. Results: Participants were 62.5% female (n = 125), with a mean age of 22.22 ± 2.08 years; 75.5% reported brushing twice daily. Anxiety scores differed significantly across academic years (MDAS: H = 39.05, ε² = 0.184, p < 0.001; MK-DAS: H = 43.07, ε² = 0.200, p < 0.001). Second-year students had the highest mean scores (MDAS: 14.08 ± 4.53; MK-DAS: 18.40 ± 4.83), whereas fourth-year students had the lowest MDAS scores (8.43 ± 3.37). High anxiety prevalence was 23.0% (MDAS) and 29.0% (MK-DAS). The scales were strongly correlated (Spearman’s ρ = 0.784, p < 0.001). Gender-related differences were significant only in second-year students. The association between academic year and anxiety scores remained significant after adjustment. Conclusions: Dental anxiety demonstrated a non-linear distribution across academic years, peaking in second-year students. MK-DAS showed strong concordance with MDAS. Findings should be interpreted cautiously given the cross-sectional, web-based design. Full article

Background: Adolescence is a sensitive period for brain maturation during which exposure to Δ⁹-tetrahydrocannabinol (THC) can induce long-lasting neurobehavioral alterations. Yet, preclinical and clinical studies report inconsistent long-term outcomes of adolescent THC exposure, ranging from clear impairments to apparently normalizing effects. We hypothesize that these discrepancies reflect stable individual differences in stress-coping abilities. Methods: To test this, selectively bred Dominant (Dom; stress-resilient, risk-prone) and Submissive (Sub; stress-vulnerable, depressive-like) Sabra mice received THC or vehicle during adolescence and were assessed in adulthood. Results: Anxiety-like and exploratory behavior, measured in the hole-board test, were differentially affected by THC as a function of stress vulnerability: in Sub mice, THC increased exploration and reduced anxiety-like behavior, whereas in Dom mice THC produced the opposite pattern. Recognition memory, evaluated by the novel object recognition test, showed modest, line-dependent alterations. Sensitivity to N-methyl-D-aspartate (NMDA) receptor hypofunction, a widely used index of vulnerability to schizophrenia-like symptoms, was examined using MK-801-induced locomotion. Adolescent THC potentiated MK-801-evoked hyperlocomotion in Dom mice but attenuated it in Sub mice. Conclusions: In the context of increasing medical and recreational cannabis exposure, these in vivo findings suggest that stress-vulnerability-related traits may be an important variable to consider in future preclinical and translational studies of adolescent THC exposure. Full article

In 2018, over half of the world’s population lived in urban areas, and this figure is expected to continue to increase over the next 25 years. Water security in growing urban areas is becoming increasingly important. Current global warming can pose additional challenges for sustainable water resource management. In this study, the city of Krakow (Poland) and its water supply system were considered. The MK and Spearman tests were used to detect trends in the studied data and the residuals from the Ensemble Empirical Mode Decomposition (EEMD) method. The analyses indicate that for 1971–2020, significant increasing trends (p < 0.05) in annual air temperature (0.36–0.46 °C/decade) were accompanied by significant trends in annual precipitation, with differences in direction and intensity (approx. −8 and 30 mm/decade). Similarly, significant trends in annual river flow for the two main sources of drinking water for Krakow (the Raba and Rudawa rivers) differed in both direction and intensity (0.51 m∙s⁻¹ and −0.05 m∙s⁻¹, respectively). The study also examined trends for individual months of the year, which largely explained the observed annual trends. Furthermore, the results of cross-correlation and autocorrelation analyses suggest that the identified decreasing trend in the Rudawa flow may be partly related to the significantly reduced underground recharge in the Rudawa catchment. The information obtained in this work can be used for more realistic and sustainable water resource management and urban-water-security planning. Full article

This paper presents a proof-of-concept investigation into a novel hermetically sealed tunable-medium Extrinsic Fabry–Pérot Interferometer (EFPI) temperature sensor architecture. A series of tuneable-sensitivity EFPI temperature sensors is demonstrated, comprising a large-diameter fused silica diaphragm with a 800 $\mathrm{m}$ diameter, significantly exceeding conventional designs (typically ∼125 $\mathrm{m}$), with polished diaphragm thicknesses ranging from 28 to 49 $\mathrm{m}$, housed in hermetically sealed rigid melting point capillaries with a 1.8 mm internal diameter. By exploiting thermally induced pressure differentials generated by a tunable Krytox GPL 105 oil/air fill fraction within the sealed rigid cavity, the sensors demonstrate a continuously tuneable sensitivity design space spanning 0.45 to 190 $\mathrm{n}\mathrm{m}/\mathrm{K}$. An exact nonlinear thermal pressure model is derived and validated, replacing the linearised approximation which is shown to be inapplicable at fill fractions approaching unity. The low-sensitivity configuration (0.45 $\mathrm{n}\mathrm{m}/\mathrm{K}$) was characterised at the National Standards Authority of Ireland (NSAI) National Metrology Laboratory against ITS-90 fixed points: the Triple Point of Water (273.16 K) and the Gallium Fixed Point (302.9146 K), with traceability to the International Temperature Scale of 1990 (ITS-90), yielding an instrument-limited resolution of $<1.1$ mK, consistent with the metrological validation environment. The high-sensitivity configurations (21 and 190 $\mathrm{n}\mathrm{m}/\mathrm{K}$) were characterised on a laboratory bench, achieving instrument-limited theoretical resolutions of <24 $\mathrm{K}$ and $<2.6$$\mathrm{K}$ respectively, pending future metrological validation. The 190 $\mathrm{n}\mathrm{m}/\mathrm{K}$ sensitivity represents an improvement of approximately 21.7× over the closest directly comparable prior Citationutilised fusion splicing and manual polishing. Future development priorities include metrological validation of the high-sensitivity configurations, long-term stability characterisation, thermal cycling, and progression towards an all-glass hermetically sealed construction. Full article

Aberrant activation of c-MET signaling contributes to tumor progression and resistance to therapy in non-small-cell lung cancer (NSCLC), yet its therapeutic significance remains incompletely understood. In this study, we evaluated c-MET expression and its association with AKT activation and clinical outcomes using a tissue microarray cohort and publicly available datasets. c-MET overexpression was significantly associated with increased p-AKT expression and showed a trend toward poorer overall survival in the tissue microarray cohort, while analysis of the TCGA LUAD dataset confirmed a significant association with reduced survival (log-rank p = 0.0223; HR = 1.234, 95% CI: 1.029–1.480). Functional studies demonstrated that pharmacological inhibition of c-MET suppressed cell proliferation and induced caspase-dependent mitochondrial apoptosis in NSCLC cell lines. Mechanistically, c-MET inhibition resulted in AKT inactivation, identifying AKT as a key downstream mediator of c-MET signaling. Notably, combined inhibition of c-MET (PHA665752) and AKT (MK2206) exhibited strong synergistic effects, significantly enhancing apoptosis and reducing cell viability compared to single-agent treatments. These findings were further validated in vivo, where combination therapy markedly delayed tumor growth without significant toxicity. Collectively, our results highlight c-MET-driven AKT activation as a key oncogenic mechanism and support dual c-MET/AKT targeting as a promising therapeutic strategy for NSCLC. Full article

Positron emission tomography (PET) of muscarinic acetylcholine receptors has evolved from a receptor-mapping exercise into a potential translational tool for probing cholinergic dysfunction in neurodegenerative disease. However, the field still lacks a clear hierarchy of clinical value across receptor subtypes, tracers, and quantitative analysis strategies, including acquisition protocols and kinetic modeling approaches. In the M₂ arena, [¹⁸F]FP-TZTP has been associated with higher distribution volumes in older APOE-ε4 carriers, but the biological meaning of this signal remains uncertain in the absence of longitudinal conversion data and with quantification approaches that are difficult to implement routinely. Among M₄ ligands, [¹¹C]MK-6884 is the most advanced and reproducible tracer to date, yet its clinical evidence still rests on proof-of-concept studies, mainly in moderate-to-severe Alzheimer’s disease, with important methodological limitations. In our view, muscarinic PET should now be reframed less as a stand-alone diagnostic biomarker and more as a platform for mechanistic and pharmacodynamic studies, especially for the development and monitoring of muscarinic-positive allosteric modulators. Future progress will depend on longitudinal multicenter validation, simplified quantification pipelines, and next-generation PET systems capable of capturing tracer kinetics more efficiently. Full article

Under global warming, understanding regional drought evolution is essential for drought early warning, food security, climate adaptation, and sustainable water-resource management. This study analyzed meteorological drought in Jiangxi Province during 1961–2022 using SPI-12, SPEI-12, and EDDI-12 from the CHM_Drought high-resolution multi-index dataset. The Mann–Kendall (MK) test, Theil–Sen slope estimator, three-threshold run theory, Morlet wavelet analysis, wavelet coherence (WTC), and cross-wavelet transform (XWT) were used to examine drought trends, event characteristics, periodicity, and inter-index relationships. Results showed a widespread drying tendency. EDDI-12 exhibited a highly significant increase in 99.86% of valid resampled raster pixels, indicating enhanced atmospheric evaporative demand, while SPEI-12 and SPI-12 showed significant decreasing trends in 97.96% and 93.24% of valid pixels, respectively. Stronger drying signals were mainly distributed in central and northern Jiangxi. Run-theory analysis indicated longer-duration cumulative droughts in southern mountainous areas and frequent short-duration drought events in the Poyang Lake Plain and central-northern Jiangxi. Wavelet analysis identified a dominant interdecadal periodicity of approximately 20–21 years. WTC and XWT revealed strong in-phase coherence between SPI and SPEI, whereas SPI/SPEI and EDDI mainly showed anti-phase statistical phase relationships. From a sustainability perspective, these findings provide scientific support for multi-index drought monitoring, adaptive agricultural water allocation, drought early warning, and climate-resilient water-resource management in humid monsoon regions. Full article

Neuroblastoma is characterized by frequent involvement of bone marrow (BM) as a site of cell dissemination and spread. In this study, single-cell RNA sequencing (scRNA-seq) was used to analyze the cellular heterogeneity of a subset of metastatic BM samples collected at initial diagnosis. Comparison of the single-cell data with bulk RNA sequencing further refined the analysis. An enrichment of regulatory T cells relative to a healthy control and activation of the CD24, CD47, and CD200 “don’t eat me” signals were documented. Computational analyses highlighted communication between neuroblastoma and myeloid cells via the amyloid precursor protein (APP) and midkine (MK) signaling networks. Within neuroblastoma cells, mutually exclusive adrenergic and transitory cell states were identified, and ten sub-clusters were denoted. In addition, common and unique tumor cell antigens were investigated. CNTFR and CHRNA3, as high-ranking candidates, were validated, confirming their strong selectivity for neuroblastoma cells. Taken together, these findings support the existence of a significant tumor-dependent modulation of the BM ecosystem, which should be considered when introducing immunotherapy. Furthermore, they highlight the potential to investigate new antigens at the single-cell resolution. Full article

Intercellular communication is crucial for the coordination of skeletal muscle development. However, the intricate signaling networks that regulate chicken myogenesis are not yet fully elucidated. In this study, we utilized CellChat analysis on single-cell and single-nucleus RNA sequencing data to systematically delineate cell–cell communication patterns across five critical developmental stages of chicken skeletal muscle: embryonic day 4 (E4), day 6 (E6), day 12 (E12), day 18 (E18), and post-hatch day 30 (P30). Our findings indicate that communication architectures are highly stage-specific, with mesenchymal cells acting as the predominant signaling hub during the early embryonic stages (E4–E6), whereas fibro-adipogenic progenitors become the principal communicators during mid-to-late embryogenesis (E12–E18). At E4, the communication network was relatively simple, comprising 51 ligand–receptor pairs primarily involving the neural cell adhesion molecule, slit guidance ligand, and midkine (MK) signaling pathways between myogenic progenitors and mesenchymal cells. By E6, the network had expanded significantly, encompassing 6237 ligand–receptor pairs across 51 signaling pathways, which coincided with the emergence of multiple myogenic lineages. Peak communication complexity was observed at E12, characterized by 11,675 ligand–receptor pairs and 61 signaling pathways, reflecting the secondary wave of myogenesis. Comparative analysis across developmental stages revealed key signaling transitions: the pleiotrophin and MK pathways were predominantly active during the early phase of myogenic commitment (E4–E6), whereas the collagen, laminin, and adhesion G protein-coupled receptor L pathways were more prominent during the secondary myogenesis phase (E6–E12). Notably, a significant shift in communication patterns was observed from E12 to E18, marked by a reduction in developmental pathway signaling and an increase in immune-related communications. By P30, the communication network had stabilized into a homeostatic state, centered on interactions among myofibers, stromal cells, and the vascular system. This comprehensive atlas of intercellular communication offers novel insights into the signaling dynamics underpinning chicken skeletal muscle development. Full article

The growing demand for sustainable construction materials has led to significant advancements in ultra-high-performance concrete (UHPC), with a particular focus on geopolymer-based systems as an alternative to conventional cementitious binders. This review explores the latest developments in sustainable Ultra-High-Performance Geopolymer Concrete (UHPGPC) by analysing key material composition, mechanical, durability and microstructural properties. The incorporation of ground granulated blast furnace slag (GGBFS), silica fume (SF), and fly ash (FA) has demonstrated notable improvements in compressive strength, durability, and workability. Additionally, the use of activators such as sodium silicate and sodium hydroxide optimizes geopolymerization, resulting in a denser microstructure and enhanced mechanical performance. This review highlights the critical role of fibre reinforcement in UHPGPC, where steel fibres (SFs) and hybrid fibres significantly enhance compressive and tensile strength, as well as crack resistance. The inclusion of waste materials such as rice husk ash and recycled glass promotes sustainability by reducing CO₂ emissions while maintaining structural integrity. However, higher waste-glass content may adversely affect bonding due to its smooth surface texture. The findings highlight the potential of UHPGC as a high-performance, eco-friendly alternative to traditional cement-based UHPC. By integrating industrial by-products and alternative activation techniques, UHPGPC can contribute significantly to the global shift towards sustainable and low-carbon construction materials. Full article

Background/Objectives: Linear growth in children reflects cumulative influences of nutrition, health, and skeletal development. Vitamin K₂, particularly menaquinone-7 (MK-7), plays an important role in bone mineralization, yet evidence regarding its potential relationship with height growth in children remains limited. This study evaluated the association between continuous MK-7 supplementation and longitudinal height growth in children. Methods: A longitudinal observational study was conducted among 1150 apparently healthy children aged 6–14 years in Hanoi, Vietnam (2022–2025), including 613 controls and 537 children receiving MK-7 supplementation. MK-7 was administered orally at 360 µg/day from baseline throughout follow-up. A total of 3491 repeated height measurements were collected. Analyses were stratified according to pubertal stage (no-puberty and pre-puberty). Height gain was summarized according to follow-up duration, and initial mixed-effects models were used to explore longitudinal growth trajectories. Because substantial follow-up imbalance was observed after the first follow-up assessment, the primary regression analyses were subsequently restricted to baseline and first follow-up observations. Multivariable linear regression models evaluated the interaction between MK-7 supplementation and follow-up duration after adjustment for age, sex, baseline body mass index-for-age Z-score, early sleep, and physical activity. Results: Height gain increased significantly with follow-up duration across all analyses (β range: 0.49–0.58 cm/month; all p < 0.001). MK-7 supplementation alone was not independently associated with height gain; however, positive interactions between MK-7 supplementation and follow-up duration were observed in several subgroup analyses. In the overall cohort, the interaction estimate was β = 0.05 cm/month (95% CI: 0.02–0.09). Positive interactions were observed in no-puberty children (β = 0.05; 95% CI: 0.01–0.09) and pre-puberty children (β = 0.06; 95% CI: 0.03–0.09). The largest interaction estimate was observed among pre-puberty boys (β = 0.10; 95% CI: 0.07–0.13), whereas no statistically significant interaction was observed among girls in the pre-puberty subgroup. Conclusions: The findings suggest that continuous MK-7 supplementation may be associated with progressively greater height gain over time rather than an immediate increase in height. The observed associations appeared more evident with longer follow-up duration, particularly among pre-pubertal boys. However, given the observational design and substantial follow-up imbalance between groups, the findings should be interpreted cautiously. Further prospective studies with balanced longitudinal follow-up are needed to clarify the potential relationship between MK-7 supplementation and pediatric linear growth. Full article

Acanthamoeba keratitis (AK) and mycotic keratitis (MK) are severe infectious diseases of the cornea. A major challenge to treatment is the poor bioavailability of conventional eye drop formulations, resulting in significant loss of the therapeutic compound. The present study describes the preparation of self-nanoemulsifying drug delivery systems (SNEDDSs) utilizing 3D-molded inserts as a vehicle for the co-delivery of voriconazole (VOR) and diclofenac sodium (DIC). Characterization of the SNEDDS and the 3D-molded inserts involved analysis of droplet size, polydispersity index (PDI), and zeta potential (ZP), followed by an ex vivo transcorneal permeation. Careful optimization of the SNEDDS composition was fundamental for obtaining a nanoemulsion with a droplet size under 100 nm, a PDI below 0.35, and a variable ZP (ranging from −14.6 ± 0.1 to 16.3 ± 2.4 mV). These properties facilitated a marked improvement in voriconazole’s solubility and subsequent transcorneal permeability. The investigation of the inserts revealed that the in vitro drug release could be tailored for immediate release or extended release. Moreover, ex vivo permeation studies demonstrated that the inserts delivered voriconazole to the cornea at concentrations more than the minimum inhibitory concentration. This work successfully demonstrates the formulation of a patient-centric, personalized drug delivery platform for the treatment of AK and MK. Full article

Amyotrophic lateral sclerosis (ALS) remains an intractable motor neuron (MN) disease with a growing patient population and few effective treatments. Here, we review how extracellular phosphoglycerate kinase 1 (ePgk1) improves neurite outgrowth of MNs (NOMN) and axonal growth, both in vitro and in vivo. Our group first elucidated a novel non-canonical function of ePgk1 as a cross-tissue mediator between nerve and muscle tissues. We then discovered that neural membranous Enolase 2 (Eno2) serves as a receptor of ligand ePgk1 and that ePgk1-Eno2 interaction suppresses the Rac1-GTP/p-Pak1-T423/p-P38-T180/pMK2-T334/p-Limk1-S323 axis, reducing p-Cofilin and promoting NOMN and axonal growth, finally suggesting that the 419th aspartic acid residue of Eno2 mediates this interaction. In a crucial preclinical step, we truncated two short 16-amino-acid derivatives from Pgk1, FD-1/-2, each mediating neuroprotection comparable to that of full-length 417-amino-acid Pgk1 in ALS animal models, in terms of improvements of innervated neuromuscular junction, MN cell bodies, motor performance, and endpoint prolongation. In this context, we also discuss the opposite function driven by Eno1-plasminogen interaction and by Eno2-ePgk1 interaction; the latter results in unfavorable for tumorigenesis. Unlike intracellular Pgk1 roles, ePgk1 is an extracellular factor with anti-angiogenic properties, further positioning ePgk1 and its FD-1/-2 as promising protein/peptide drugs for ALS treatment. Full article