Search Results (31)

Luffa fruit is an underutilized and novel ingredient in North America. To increase the shelf life of luffa fruit, this study evaluated the creation of luffa fruit powder using three different drying temperatures (40 °C, 50 °C, and 60 °C). The objective of this study was to evaluate the sensory properties and acceptability of luffa fruit powder with unfamiliar consumers (those who do not regularly eat luffa fruit). Participants (n = 88) evaluated the luffa fruit powders mixed into couscous, as well as a control (couscous without luffa fruit powder) using check-all-that-apply and hedonic scales. Furthermore, the participants were asked how they felt about luffa fruit powder after evaluating the samples (comment question). The hedonic scores were not significantly affected by the addition of the luffa seed powders dried at different temperatures. However, the luffa seed powder was associated with a mild flavour, as well as being described as earthy and vegetal. The participants did indicate that the luffa seed powder added moistness to the couscous. As the drying temperature increased so did the intensity of the flavour. Furthermore, participants indicated they would be interested in luffa seed powder if it has nutritional benefits. Overall, this study investigated the sensory properties of a novel ingredient, luffa seed powder, and future studies should continue to explore its sensory properties and chemical components. Full article

In the current context of environmental concerns and the search for sustainable food solutions, this study investigated the valorization of Luffa cylindrica seed press cake, a waste byproduct from oil extraction, as a functional ingredient for meat substitute formulations. The research systematically characterized the functional and bioactive properties of L. cylindrica seed press cake powder (LP) and its blends with tapioca flour (TF) at ratios of 30–70%. Techno-functional analyses included: hydration properties (water holding capacity, water absorption capacity, water absorption index, water solubility index, swelling power, oil absorption capacity); rheological characteristics; bioactive profiling through antioxidant assays (DPPH, ABTS, FRAP); and reducing sugar content determination. Meat substitute formulations were developed using an LP30/TF70 blend combined with coral lentils, red beet powder, and water, followed by a sensory evaluation and storage stability assessment. Pure L. cylindrica powder exhibited the highest water holding capacity (3.62 g H₂O/g) and reducing sugar content (8.05 mg GE/g), while tapioca flour showed superior swelling properties. The blends demonstrated complementary functional characteristics, with the LP30/TF70 formulation selected for meat substitute development based on optimal textural properties. The sensory evaluation revealed significant gender differences in acceptance, with women rating the product substantially higher than men across all attributes. The study successfully demonstrated the feasibility of transforming agricultural waste into a valuable functional ingredient, contributing to sustainable food production and representing the first comprehensive evaluation of L. cylindrica seed press cake for food applications. However, the study revealed limitations, including significant antioxidant loss during thermal processing (80–85% reduction); a preliminary sensory evaluation with limited participants showing gender-dependent acceptance; and a reliance on locally available tapioca flour, which may limit global applicability. Future research should focus on processing optimization to preserve bioactive compounds, comprehensive sensory studies with diverse populations, and an investigation of alternative starch sources to enhance the worldwide implementation of this valorization approach. Full article

Zeugodacus cucuribitae (Coquillett) (Z. cucuribitae) is a global extremely invasive quarantine pest which has a wide host range of fruits and vegetables. At present, there are a few control measures for Z. cucuribitae, and deltamethrin and avermectin are commonly used. Among the hosts of Z. cucuribitae, Luffa acutangular, Luffa cylindrica, Sechium edule, Brassica oleracea var. botrytis, Musa nana, and Fragaria × ananassa are non-favored hosts. However, it is still not clear why these hosts are non-favored and whether there are any repellent components of Z. cucuribitae in these hosts. In this study, the components of these six hosts were collected from the literature, and the genes of odor and chemical sensation were determined from the genome of Z. cucuribitae. After the potential relationships between these components and genes were determined by molecular docking methods, the KEGG and GO enrichment analysis of these genes was conducted, and a complex network of genes vs. components vs. Kegg pathway vs. GO terms was constructed and used to select the key components for experiments. The results show that oleanolic acid (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL), rotenone (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL), and beta-caryophyllene oxide (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL) had a significant repellent effect on Z. cucuribitae, and three components, rotenone (1 mg/mL and 0.1 mg/mL), echinocystic acid (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL), and beta-caryophyllene oxide (1 mg/mL, and 0.1 mg/mL) had significant stomach toxicity in Z. cucuribitae. Furthermore, a complex signaling pathway was built and used to predict the effect of these components on Z. cucuribitae. These components probably play roles in the neuroactive ligand–receptor interaction (ko04080) and calcium signaling (ko04020) pathways. This study provides a reference for the prevention and control of Z. cucuribitae and a scientific reference for the rapid screening and development of new pest control drugs. Full article

This study presents the first comprehensive nationwide assessment of mangrove ecosystems in the Maldives. Surveys were conducted across 162 islands in 20 administrative atolls, integrating field data, the literature, and secondary sources to map mangrove distribution, confirm species presence, and classify habitat types. Twelve true mangrove species were identified, with Bruguiera cylindrica, Rhizophora mucronata, and Lumnitzera racemosa emerging as dominant. Species diversity was evaluated using Shannon (H′), Margalef (d′), Pielou’s evenness (J′), and Simpson’s dominance (λ′) indices. Atolls within the northern and southern regions, particularly Laamu, Noonu, and Shaviyani, exhibited the highest diversity and evenness, while central atolls such as Ari and Faafu supported mono-specific or degraded stands. Mangrove habitats were classified into four geomorphological types: marsh based, pond based, embayment, and fringing systems. Field sampling was conducted using standardized belt transects and quadrats, with species verified using photographic documentation and expert validation. Species distributions showed strong habitat associations, with B. cylindrica dominant in marshes, R. mucronata and B. gymnorrhiza in ponds, and Ceriops tagal and L. racemosa in embayments. Rare species like Bruguiera hainesii and Heritiera littoralis were confined to stable hydrological niches. This study establishes a critical, island-level baseline for mangrove conservation and ecosystem-based planning in the Maldives, providing a reference point for tracking future responses to climate change, sea-level rise, and hydrological disturbances, emphasizing the need for habitat-specific strategies to protect biodiversity. Full article

To investigate the immunomodulatory activity of polysaccharides derived from the rhizome of Imperata cylindrica, polysaccharides (IRPs-H) were extracted using hot water extraction and further purified via DEAE-52 ion-exchange chromatography, yielding three fractions: IRPs-H1, IRPs-H2, and IRPs-H3. The structural features of these fractions were characterized by Fourier-transform infrared spectroscopy (FT-IR), high-performance gel permeation chromatography (HPGPC), atomic force microscopy (AFM), and thermogravimetric analysis (TGA). Their immunological activities were evaluated in vitro. All three fractions were identified as neutral pyranose-type polysaccharides, primarily composed of glucose and xylose, exhibiting good thermal stability and lacking long-chain structures. In vitro assays using RAW264.7 macrophages demonstrated that these polysaccharides promoted cell proliferation (50–800 μg/mL), enhanced phagocytic activity, and induced morphological changes characteristic of macrophage activation, including irregular shapes and pseudopod formation. ELISA and flow cytometry analyses revealed dose-dependent increases in nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and reactive oxygen species (ROS) levels. Notably, the IRPs-H3 fraction stimulated TNF-α and IL-6 production to levels of 438.02 ± 14.14 pg/mL and 30.13 ± 1.27 pg/mL, respectively, which were comparable to those induced by lipopolysaccharide (LPS), the positive control (460.83 ± 16.10 pg/mL and 31.87 ± 1.72 pg/mL, respectively). These results suggest that polysaccharides extracted from the rhizome of Imperata cylindrica possess significant immunostimulatory properties and hold potential for development as functional food ingredients or immune-enhancing agents. Full article

The use of natural materials as adsorbents and the environmentally friendly removal of pollutants and azo dyes from water are important topics today. The goal of this research work was to assess the utility of Luffa cylindrica (L. cylindrica) as a natural and non-conventional adsorbent for azo dyes in water on a large scale (2 L). An azo dye (AD) at a concentration of 0.250 g/L was removed from the solution at a rate of 63.07% using 10.0 g/L doses of L. cylindrica, and the maximum adsorption capacity of L. cylindrica was 25.25 mg/g. L. cylindrica desorbed 95.8% of the AD in 0.1 M NaOH. Thermodynamically, the adsorption occurs through pseudo-second-order kinetics and the behaviors adjust better to the Langmuir isotherm. The analysis of variance (p-value < 0.05) shows that the contact time and the concentration of AD significantly influence the adsorption capacity and removal of AD. Few studies have examined the environmentally friendly removal of azo dyes from water using a natural non-conventional adsorbent. Full article

Phycobiliproteins and pigments derived from cyanobacteria hold significant potential for diverse applications in the food, pharmaceutical, and chemical industries. The filamentous cyanobacterium Anabaena cylindrica serves as a valuable resource for extracting these compounds. This study develops a simplified, safe, and cost-effective extraction method that eliminates toxic solvents and minimizes processing steps. This makes the method applicable for all users and allows the easy integration of the extraction into biorefinery concepts in which the biomass is to be used as a fertilizer, for example. Utilizing salts such as ammonium sulfate and calcium chloride (15 gL⁻¹ each) enables the effective extraction of phycocyanin (PC) and allophycocyanin, achieving a PC concentration of 192.34 $\mathrm{mg}{\mathrm{g}}_{\mathrm{CDW}}^{-1}$ and 209.44 $\mathrm{mg}{\mathrm{g}}_{\mathrm{CDW}}^{-1}$, respectively. Ethanol was introduced as a less toxic alternative to methanol for pigment extraction, increasing chlorophyll a and carotenoid recovery by 21% and 37%, respectively. Full article

The pyrabactin resistance 1-like (PYR/PYL) proteins are abscisic acid receptors that perform multiple functions in various plant growth and development processes. However, the PYR/PYL gene family in luffa (Luffa cylindrica L.) has not been well-explored. In this study, we analysed the effects of whole-genome member identification, endogenous soluble sugars (SS), soluble proteins (SP), abscisic acid (ABA), indole-3-acetic acid (IAA, auxin) and the gene expression pattern of PYR/PYL influenced by exogenous abscisic acid (ABA) during the fruit development of luffa through the use of physiological and biochemical analyses, bioinformatics, and RT-qPCR techniques. We conducted a comprehensive genome-wide identification and characterisation of the PYR/PYL gene family in luffa fruit development. Four LcPYR and 10 LcPYL genes were identified in the luffa reference genome via bioinformatics analyses. A chromosomal mapping of the identified LcPYR/PYL genes showed that they were distributed on 9 of the 13 chromosomes in the luffa genome. Conserved structural domain analyses of the 14 proteins encoded by the LcPYR/PYL genes identified the PYR_PYL_RCAR_like structural domains typical of this family; however, no regulatory component of abscisic acid receptor (RCAR)-type genes was found. At six luffa fruit development stages (i.e., 0, 3, 6, 9, 12, and 15 days after pollination), the contents of soluble sugars, soluble proteins, and endogenous hormones ABA and IAA in the fruit significantly increased. Under the exogenous ABA treatments, the contents of these four endogenous substances in the fruits were significantly higher than they were in the control group at the same time period, and ABA and IAA seemed to be synergistically involved in the luffa fruit-ripening process. An analysis of the luffa transcriptome data and real-time fluorescence quantitative PCR (RT-qPCR) experiments showed that multiple LcPYR/PYLs (e.g., LcPYL10 and LcPYR4) had differential expression levels in the seven different tissues and exogenous ABA-treated fruits that were analysed, suggesting their roles in ABA hormone-mediated ripening of luffa fruit. Together, the results provide basic information about the LcPYR/PYL family in L. cylindrica and their involvement in fruit development. Full article

Cyclic nucleotide-gated ion channels (CNGCs) are cell membrane channel proteins for calcium ions. They have been reported to play important roles in survival and in the responses to environmental factors in various plants. However, little is known about the CNGC family and its functions in luffa (Luffa cylindrica L.). In this study, a bioinformatics-based method was used to identify members of the CNGC gene family in L. cylindrica. In total, 20 LcCNGCs were detected, and they were grouped into five subfamilies (I, II, Ⅲ, IV-a, and IV-b) in a phylogenetic analysis with CNGCs from Arabidopsis thaliana (20 AtCNGCs) and Momordica charantia (17 McCNGCs). The 20 LcCNGC genes were unevenly distributed on 11 of the 13 chromosomes in luffa, with none on Chromosomes 1 and 5. The members of each subfamily encoded proteins with highly conserved functional domains. An evolutionary analysis of CNGCs in luffa revealed three gene losses and a motif deletion. An examination of gene replication events during evolution indicated that two tandemly duplicated gene pairs were the primary driving force behind the evolution of the LcCNGC gene family. PlantCARE analyses of the LcCNGC promoter regions revealed various cis-regulatory elements, including those responsive to plant hormones (abscisic acid, methyl jasmonate, and salicylic acid) and abiotic stresses (light, drought, and low temperature). The presence of these cis-acting elements suggested that the encoded CNGC proteins may be involved in stress responses, as well as growth and development. Transcriptome sequencing (RNA-seq) analyses revealed tissue-specific expression patterns of LcCNGCs in various plant parts (roots, stems, leaves, flowers, and fruit) and the upregulation of some LcCNGCs under low-temperature stress. To confirm the accuracy of the RNA-seq data, 10 cold-responsive LcCNGC genes were selected for verification by quantitative real-time polymerase chain reaction (RT-qPCR) analysis. Under cold conditions, LcCNGC4 was highly upregulated (>50-fold increase in its transcript levels), and LcCNGC3, LcCNGC6, and LcCNGC13 were upregulated approximately 10-fold. Our findings provide new information about the evolution of the CNGC family in L. cylindrica and provide insights into the functions of the encoded CNGC proteins. Full article

Megalurothrips usitatus (Bagnall) feeds on the young tissues of plants, causing wilting and deformity of leaves as well as damage to flowers and fruits, thereby seriously affecting plant yield. Due to its small size and difficulty of control, the species is one of the most important pests in the legume family. To clarify the occurrence and damage pattern of M. usitatus on field crops, a study was conducted using eight different crops as experimental materials, and an age-stage, two-sex life table was constructed. The population density of M. usitatus was the highest on cowpeas (Vigna unguiculata L. Walp.), followed by green beans (Phaseolus vulgaris L.) and Vigna cylindrica (Vigna unguiculata subsp. cylindrica (L.) Verdc.) in terms of net reproductive rate and the intrinsic rate of increase. In terms of reproductive capacity, cowpeas and V. cylindrica were more suitable for M. usitatus growth and reproduction, followed by green beans. M. usitatus could develop into adults on courgettes (Cucurbita pepo L.), wax gourds (Benincasa hispida Thunb. Cogn.), Momordica charantia (Momordica charantia L.), and soybeans (Glycine max L. Merr.) but had difficulty reproducing on those plants. Cucumber (Cucumis sativus L.) was the least suitable for the survival of M. usitatus. Population parameter analysis showed that the cowpea was the most suitable host plant for M. usitatus, followed by green beans and V. cylindrica. M. usitatus had difficulty reproducing on courgette, wax gourd, M. charantia, and soybeans, and feeding on cucumbers inhibited M. usitatus development and reproduction. Full article

Bottlenecks, including limited genetic variation and the ongoing loss of genetic diversity, have hindered the development of modern wheat cultivars., making it crucial to use genetic diversity from wild relatives to improve wheat’s adaptation to abiotic stress, such as salinity. This study assessed the phenotypic and epigenetic variation of introgressed wheat lines (BC₄F₂) derived from hybridizing two wheat cultivars with Aegilops cylindrica (AC). This study assessed the phenotypic and epigenetic variation of 156 introgressed wheat lines (BC₄F₂) derived from hybridization between wheat cultivars “Chinese Spring” (CS) and “Roshan” (R) and Aegilops cylindrica (AC). These lines and their recurrent parents (total of 158) were evaluated under normal and saline field conditions for the agronomic traits and stress tolerance indices. The data were used to select the most tolerant and most sensitive lines. Then, the selected BC₄F₂ lines and their parents (AC, CS, and R) were subjected to physiological, DNA cytosine methylation, and expression analysis of HKT1;5, NHX1, and SOS1 genes under control and salt stress conditions. Agro-physiological, epigenetic, and gene expression analyses showed the significant effects of salt stress and genetic background, as well as the differential response of the BC₄F₂ lines to salt stress. The variations in leaf and root K, Na, and K/Na ratios, and leaf Chla, Chlb, Car, and MDA levels, unlike DPPH radical scavenging levels, between salt-tolerant and salt-sensitive BC₄F₂ lines under saline conditions indicated a substantial distinction in salinity tolerance responses. RT-qPCR indicated higher expression levels of NHX1 and SOS1 genes in the leaf and root tissues of tolerant lines than those of sensitive lines. Global leaf and root DNA methylation analysis revealed the significant effects of salinity on the methylation modifications and confirmed the successful introgression of the salt-tolerance epigenome from Ae. cylindrica into wheat. Exploiting the genetic diversity of wild wheat relatives is a crucial goal for increasing genetic and epigenetic variation to enhance plant adaptation to salt stress. Full article

Luffa spp. is an important worldwide cultivated vegetable and medicinal plant from the Cucurbitaceae family. In this study, we report a high-quality chromosome-level genome of the high-generation inbred line SG261 of Luffa acutangula. The genomic sequence was determined by PacBio long reads, Hi-C sequencing reads, and 10× Genomics sequencing, with an assembly size of 739.82 Mb, contig N50 of 18.38 Mb, and scaffold N50 of 56.08 Mb. The genome of L. acutangula SG261 was predicted to contain 27,312 protein-coding genes and 72.56% repetitive sequences, of which long terminal repeats (LTRs) were an important form of repetitive sequences, accounting for 67.84% of the genome. Phylogenetic analysis reveals that L. acutangula evolved later than Luffa cylindrica, and Luffa is closely related to Momodica charantia. Comparing the genome of L. acutangula SG261 and L. cylindrica with PacBio data, 67,128 high-quality structural variations (SVs) and 55,978 presence-absence variations (PAVs) were identified in SG261, resulting in 2424 and 1094 genes with variation in the CDS region, respectively, and there are 287 identical genes affected by two different structural variation analyses. In addition, we found that the transcription factor FY (FLOWERING LOCUS Y) families had a large expansion in L. acutangula SG261 (flowering in the morning) compared to L. cylindrica (flowering in the afternoon), which may result in the early flowering time in L. acutangula SG261. This study provides valuable reference for the breeding of and pan-genome research into Luffa species. Full article

Reducing high concentrations of pollutants such as heavy metals, pesticides, drugs, and dyes from water is an emerging necessity. We evaluated the use of Luffa cylindrica (Lc) as a natural non-conventional adsorbent to remove azo dye mixture (ADM) from water. The capacity of Lc at three different doses (2.5, 5.0, and 10.0 g/L) was evaluated using three concentrations of azo dyes (0.125, 0.250, and 0.500 g/L). The removal percent (R%), maximum adsorption capacity (Q_m), isotherm and kinetics adsorption models, and pH influence were evaluated, and Fourier-transform infrared spectroscopy and scanning electron microscopy were performed. The maximum R% was 70.8% for 10.0 g L⁻¹ Lc and 0.125 g L⁻¹ ADM. The Q_m of Lc was 161.29 mg g⁻¹. Adsorption by Lc obeys a Langmuir isotherm and occurs through the pseudo-second-order kinetic model. Statistical analysis showed that the adsorbent dose, the azo dye concentration, and contact time significantly influenced R% and the adsorption capacity. These findings indicate that Lc could be used as a natural non-conventional adsorbent to reduce ADM in water, and it has a potential application in the pretreatment of wastewaters. Full article

The plant-specific WRKY transcription factor family members have diverse regulatory effects on the genes associated with many plant processes. Although the WRKY proteins in Arabidopsis thaliana and other species have been thoroughly investigated, there has been relatively little research on the WRKY family in Luffa cylindrica, which is one of the most widely grown vegetables in China. In this study, we performed a genome-wide analysis to identify L. cylindrica WRKY genes, which were subsequently classified and examined in terms of their gene structures, chromosomal locations, promoter cis-acting elements, and responses to abiotic stress. A total of 62 LcWRKY genes (471–2238 bp) were identified and divided into three phylogenetic groups (I, II, and III), with group II further divided into five subgroups (IIa, IIb, IIc, IId, and IIe) in accordance with the classification in other plants. The LcWRKY genes were unevenly distributed across 13 chromosomes. The gene structure analysis indicated that the LcWRKY genes contained 0–11 introns (average of 4.4). Moreover, 20 motifs were detected in the LcWRKY proteins with conserved motifs among the different phylogenetic groups. Two subgroup IIc members (LcWRKY16 and LcWRKY31) contained the WRKY sequence variant WRKYGKK. Additionally, nine cis-acting elements related to diverse responses to environmental stimuli were identified in the LcWRKY promoters. The subcellular localization analysis indicated that three LcWRKY proteins (LcWRKY43, LcWRKY7, and LcWRKY23) are localized in the nucleus. The tissue-specific LcWRKY expression profiles reflected the diversity in LcWRKY expression. The RNA-seq data revealed the effects of low-temperature stress on LcWRKY expression. The cold-induced changes in expression were verified via a qRT-PCR analysis of 24 differentially expressed WRKY genes. Both LcWRKY7 and LcWRKY12 were highly responsive to the low-temperature treatment (approximately 110-fold increase in expression). Furthermore, the LcWRKY8, LcWRKY12, and LcWRKY59 expression levels increased by more than 25-fold under cold conditions. Our findings will help clarify the evolution of the luffa WRKY family while also providing valuable insights for future studies on WRKY functions. Full article

Cyanobacteria are promising organisms for the sustainable production of various biotechnological interesting products. Due to their energy production via photosynthesis, the cultivation of cyanobacteria expands the CO₂ cycle. Most cyanobacteria form biofilms on surfaces in their natural environment by surrounding the cells with a self-produced matrix of extracellular polymeric substances (EPS) that hold the cells together. These special growth properties need special reactors for cultivation. By immobilizing cyanobacteria on carriers, systems currently established in industry could also be used for biofilm formers. Various artificial carriers for immobilized growth of cyanobacteria and microalgae have already been described in the literature. However, the use of waste materials or natural biodegradable carriers would be more sustainable and is, therefore, the focus of this study. Dried Luffa cylindrica, zeolite, and corn stalks were investigated for their use as carriers for cyanobacteria. L. cylindrica was shown to be an excellent natural carrier for (i) Anabaena cylindrica, (ii) Nostoc muscorum 1453-12a, and (iii) Nostoc muscorum 1453-12b. Higher or at least similar growth rates were achieved when cyanobacteria were cultivated with L. cylindrica compared to submerged cultivation. Additionally, the production of EPS and C-phycocyanin was increased at least 1.4 fold in all strains by culturing on L. cylindrica. The improved growth could be explained on the one hand by the high surface area of L. cylindrica and its properties, and, on the other hand, by the release of growth-promoting nutrients from L. cylindrica to the medium. Full article