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25 pages, 7475 KiB  
Article
Human Dialyzable Leukocyte Extract Enhances Albendazole Efficacy and Promotes Th1/Th2-Biased Lymphocyte and Antibody Responses in Peritoneal Cavity of Murine Model of Mesocestoides vogae Infection
by Gabriela Hrčková, Dagmar Mudroňová, Katarína Reiterová, Serena Cavallero and Ilaria Bellini
Int. J. Mol. Sci. 2025, 26(14), 6994; https://doi.org/10.3390/ijms26146994 - 21 Jul 2025
Viewed by 195
Abstract
Human leukocyte extract (HLE), a non-immunogenic dialyzable leukocyte preparation (<10 kDa), may serve as a safe adjuvant in immunotherapy. We investigated the effects of albendazole (ABZ), HLE, and their combination in Mesocestoides vogae infected mice, focusing on lymphoid cells in the peritoneal cavity, [...] Read more.
Human leukocyte extract (HLE), a non-immunogenic dialyzable leukocyte preparation (<10 kDa), may serve as a safe adjuvant in immunotherapy. We investigated the effects of albendazole (ABZ), HLE, and their combination in Mesocestoides vogae infected mice, focusing on lymphoid cells in the peritoneal cavity, the site of larval proliferation and parasite-induced immunosuppression. Peritoneal lymphoid cells were analysed by flow cytometry and qPCR. Cells proliferative responses to ConA, LPS, and parasite excretory/secretory (E/S) antigens, cytokine production (ELISA), IgM and IgG isotypes in exudates and parasite antigen recognition (Western blot) were assessed. Efficacy was measured by larval burden and 14-3-3 gene expression in larvae. HLE combined with ABZ enhanced larval clearance and suppressed 14-3-3 gene expression in larvae. HLE and combination therapy increased CD3+ T cell frequencies, especially CD3+high, reduced regulatory CD3+/IL-10 Tregs and expression of Foxp3+. All treatments diminished CD19+/IL-10+ Bregs, correlating with lower CD9 and Atf3 mRNA levels compared to infected mice. Transcription factors T-bet expression was strongly upregulated, while GATA3 was moderately elevated. IFN-γ production and T/B cell proliferation were restored after HLE and combination therapy, partially, even in the presence of E/S antigens. IgM and total IgG levels against parasite antigens declined, while Th1-associated IgG2a increased in ABZ+HLE and HLE-treated groups. Albendazole failed to reverse the immunosuppressive Treg-type immunity but was more effective in reducing Breg populations and their functions. HLE enhanced ABZ efficacy by restoring Th1 responsiveness, reducing Treg/Breg activity, and modulating antibody profiles. It represents a promising immunomodulatory adjuvant in the treatment of the infections associated with Th2/Treg-driven immunosuppression. Full article
(This article belongs to the Special Issue Molecular Research on Parasitic Infection)
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17 pages, 3500 KiB  
Article
Protective Effect of Field Horsetail Polyphenolic Extract on Erythrocytes and Their Membranes
by Katarzyna Męczarska, Sylwia Cyboran-Mikołajczyk, Katarzyna Solarska-Ściuk, Jan Oszmiański, Katarzyna Siejak and Dorota Bonarska-Kujawa
Int. J. Mol. Sci. 2025, 26(7), 3213; https://doi.org/10.3390/ijms26073213 - 30 Mar 2025
Cited by 2 | Viewed by 1025
Abstract
Field horsetail (Equisetum arvense L.) is widely utilized in traditional medicine and is a rich source of bioactive compounds such as flavonoids, phenolic acids, and silica. This study investigates the protective effect of the polyphenolic extract from field horsetail (HLE) on erythrocytes [...] Read more.
Field horsetail (Equisetum arvense L.) is widely utilized in traditional medicine and is a rich source of bioactive compounds such as flavonoids, phenolic acids, and silica. This study investigates the protective effect of the polyphenolic extract from field horsetail (HLE) on erythrocytes and their cell membranes. The content of polyphenolic compounds in the extract was determined using the HPLC-DAD and Folin–Ciocalteu methods. The extract’s hemolytic activity, toxicity, antioxidant activity, and its impact on the physical properties of erythrocytes and lipid membrane were investigated. The antioxidant properties were evaluated using erythrocytes and isolated erythrocyte membranes oxidized by UVC radiation and AAPH. The impact of the extract on the ordering and fluidity of erythrocyte and model lipid membranes was studied. Furthermore, the transmembrane potential, shape of erythrocytes and the dipole potential of the lipid membranes under the influence of HLE were evaluated. The results indicated that HLE extract exhibited no toxicity to erythrocytes and HMEC-1 cells. HLE components effectively protect erythrocytes and their membranes against oxidation. They interact with the outer, polar surface of the erythrocyte membrane and reduce both erythrocyte membrane potential and lipid membrane dipole potential. The HLE polyphenols decrease the concentration of free radicals at the surface of the membrane, where they are located, and serve as a protective barrier, preventing penetration into the membrane. Full article
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17 pages, 4228 KiB  
Article
Anti-Obesity Effects Evaluation of a Blackcurrant Leaf Standardized Hydro-Alcoholic Extract in Wistar Rat Subjected to a High-Fat Diet
by Gwendoline Bréger, Agnès André, César Cotte, Abderrahim Hammaidi, Aline Amérand, Claude Faivre, Lionel Martignat and Mohamed Yassine Mallem
Biology 2024, 13(12), 999; https://doi.org/10.3390/biology13120999 - 1 Dec 2024
Viewed by 1333
Abstract
Blackcurrant (BC) extract was reported to exert anti-obesity effects. However, it is unknown whether BC extract with a composition close to the totum differentially affects obesity when compared to one of its active compounds. We evaluated the anti-obesity effects of a BC standardized [...] Read more.
Blackcurrant (BC) extract was reported to exert anti-obesity effects. However, it is unknown whether BC extract with a composition close to the totum differentially affects obesity when compared to one of its active compounds. We evaluated the anti-obesity effects of a BC standardized hydro-alcoholic leaf extract (BC-HLE) in an HFD-induced obesity rat model and compared them with quercetin (QUE). Thirty-six 12-week-old Wistar rats were divided into six groups: control, HFD, BC-HLE- (41 and 50 mg/kg) and QUE- (0.9 and 50 mg/kg) supplemented HFD rats for 12 weeks. HFD rats developed a moderate obesity, associated with a gut dysbiosis and a change in their total antioxidant capacity. The increase in body weight gain was prevented only by the low dose of BC-HLE and the high dose of QUE. The impaired glucose tolerance by HFD was attenuated by the low dose of QUE. Hepatic glutathione peroxidase activity was increased in the HFD group and only BC-HLE supplementation counteracted this change. The low BC-HLE dose tended to reduce the HFD-induced gut dysbiosis. These findings suggest that while QUE has beneficial effects on obesity-related disorders, the BC-HLE may offer even greater overall benefits and could contribute to preventing obesity and related conditions. Full article
(This article belongs to the Special Issue Physiology and Pathophysiology of Obesity)
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12 pages, 4500 KiB  
Article
Effect of Different Finishing Systems on Surface Roughness and Gloss of a 3D-Printed Material for Permanent Dental Use
by Alessandro Vichi, Dario Balestra and Chris Louca
Appl. Sci. 2024, 14(16), 7289; https://doi.org/10.3390/app14167289 - 19 Aug 2024
Cited by 3 | Viewed by 2132
Abstract
The object of the study was to assess the effect of different finishing and polishing systems on the roughness and gloss of a 3D-printed permanent restorative material. One 3D printable Permanent material was selected for the study. Squared-shaped specimens (14 mm2; [...] Read more.
The object of the study was to assess the effect of different finishing and polishing systems on the roughness and gloss of a 3D-printed permanent restorative material. One 3D printable Permanent material was selected for the study. Squared-shaped specimens (14 mm2; 5 mm thickness) were obtained by designing and printing. Eighty specimens were produced and randomly assigned (n = 10) to 8 finishing and polishing methods: Sof-Lex™ Spiral Wheels (SW), Sof-Lex™ XT Pop-on Disc (SD), Identoflex Lucent no paste (Ln), Identoflex Lucent + paste (Lp), Resin Nitrogen polymerized (NG), Optiglaze (OG), Opti1Step (OS), and HiLusterPLUS (HL). Surface roughness and gloss were then measured by a roughness meter and a glossmeter, respectively. For roughness, statistically significant differences were found (p < 0.001), with NG(a) > SD(b) = OG(b) = Lp(b); Lp(b) = Ln(bc); Ln(bc) = OS(cd); OS(cd) = SW(de); and SW(de) = HL(e). For gloss, statistically significant differences were also identified (p < 0.001) with NG(a) > SD(b) > Lp(c) = OS(c) = OG(cd); OG(cd) = Ln(d) > HL(e) = SW(e). The nitrogen chamber polymerization showed better results for both roughness and gloss. Multi-step finishing/polishing systems were able to produce smoother surfaces than 1-step and 2-step systems. Full article
(This article belongs to the Special Issue Advanced Materials for Polymeric 3D Printing Applications)
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25 pages, 4986 KiB  
Article
Estimating Disease-Free Life Expectancy Based on Clinical Data from the French Hospital Discharge Database
by Oleksandr Sorochynskyi, Quentin Guibert, Frédéric Planchet and Michaël Schwarzinger
Risks 2024, 12(6), 92; https://doi.org/10.3390/risks12060092 - 3 Jun 2024
Viewed by 1315
Abstract
The development of health indicators to measure healthy life expectancy (HLE) is an active field of research aimed at summarizing the health of a population. Although many health indicators have emerged in the literature as critical metrics in public health assessments, the methods [...] Read more.
The development of health indicators to measure healthy life expectancy (HLE) is an active field of research aimed at summarizing the health of a population. Although many health indicators have emerged in the literature as critical metrics in public health assessments, the methods and data to conduct this evaluation vary considerably in nature and quality. Traditionally, health data collection relies on population surveys. However, these studies, typically of limited size, encompass only a small yet representative segment of the population. This limitation can necessitate the separate estimation of incidence and mortality rates, significantly restricting the available analysis methods. In this article, we leverage an extract from the French National Hospital Discharge database to define health indicators. Our analysis focuses on the resulting Disease-Free Life Expectancy (Dis-FLE) indicator, which provides insights based on the hospital trajectory of each patient admitted to hospital in France during 2008–2013. Through this research, we illustrate the advantages and disadvantages of employing large clinical datasets as the foundation for more robust health indicators. We shed light on the opportunities that such data offer for a more comprehensive understanding of the health status of a population. In particular, we estimate age-dependent hazard rates associated with sex, alcohol abuse, tobacco consumption, and obesity, as well as geographic location. Simultaneously, we delve into the challenges and limitations that arise when adopting such a data-driven approach. Full article
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22 pages, 11434 KiB  
Article
Noncontact Automatic Water-Level Assessment and Prediction in an Urban Water Stream Channel of a Volcanic Island Using Deep Learning
by Fábio Mendonça, Sheikh Shanawaz Mostafa, Fernando Morgado-Dias, Joaquim Amândio Azevedo, Antonio G. Ravelo-García and Juan L. Navarro-Mesa
Electronics 2024, 13(6), 1145; https://doi.org/10.3390/electronics13061145 - 20 Mar 2024
Viewed by 1684
Abstract
Traditional methods for water-level measurement usually employ permanent structures, such as a scale built into the water system, which is costly and laborious and can wash away with water. This research proposes a low-cost, automatic water-level estimator that can appraise the level without [...] Read more.
Traditional methods for water-level measurement usually employ permanent structures, such as a scale built into the water system, which is costly and laborious and can wash away with water. This research proposes a low-cost, automatic water-level estimator that can appraise the level without disturbing water flow or affecting the environment. The estimator was developed for urban areas of a volcanic island water channel, using machine learning to evaluate images captured by a low-cost remote monitoring system. For this purpose, images from over one year were collected. For better performance, captured images were processed by converting them to a proposed color space, named HLE, composed of hue, lightness, and edge. Multiple residual neural network architectures were examined. The best-performing model was ResNeXt, which achieved a mean absolute error of 1.14 cm using squeeze and excitation and data augmentation. An explainability analysis was carried out for transparency and a visual explanation. In addition, models were developed to predict water levels. Three models successfully forecasted the subsequent water levels for 10, 60, and 120 min, with mean absolute errors of 1.76 cm, 2.09 cm, and 2.34 cm, respectively. The models could follow slow and fast transitions, leading to a potential flooding risk-assessment mechanism. Full article
(This article belongs to the Special Issue Selected Papers from Young Researchers in AI for Computer Vision)
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20 pages, 9259 KiB  
Article
A Cataract-Causing Mutation in the TRPM3 Cation Channel Disrupts Calcium Dynamics in the Lens
by Yuefang Zhou, Thomas M. Bennett, Philip A. Ruzycki, Zhaohua Guo, Yu-Qing Cao, Mohammad Shahidullah, Nicholas A. Delamere and Alan Shiels
Cells 2024, 13(3), 257; https://doi.org/10.3390/cells13030257 - 30 Jan 2024
Cited by 2 | Viewed by 2032
Abstract
TRPM3 belongs to the melastatin sub-family of transient receptor potential (TRPM) cation channels and has been shown to function as a steroid-activated, heat-sensitive calcium ion (Ca2+) channel. A missense substitution (p.I65M) in the TRPM3 gene of humans (TRPM3) and [...] Read more.
TRPM3 belongs to the melastatin sub-family of transient receptor potential (TRPM) cation channels and has been shown to function as a steroid-activated, heat-sensitive calcium ion (Ca2+) channel. A missense substitution (p.I65M) in the TRPM3 gene of humans (TRPM3) and mice (Trpm3) has been shown to underlie an inherited form of early-onset, progressive cataract. Here, we model the pathogenetic effects of this cataract-causing mutation using ‘knock-in’ mutant mice and human cell lines. Trpm3 and its intron-hosted micro-RNA gene (Mir204) were strongly co-expressed in the lens epithelium and other non-pigmented and pigmented ocular epithelia. Homozygous Trpm3-mutant lenses displayed elevated cytosolic Ca2+ levels and an imbalance of sodium (Na+) and potassium (K+) ions coupled with increased water content. Homozygous TRPM3-mutant human lens epithelial (HLE-B3) cell lines and Trpm3-mutant lenses exhibited increased levels of phosphorylated mitogen-activated protein kinase 1/extracellular signal-regulated kinase 2 (MAPK1/ERK2/p42) and MAPK3/ERK1/p44. Mutant TRPM3-M65 channels displayed an increased sensitivity to external Ca2+ concentration and an altered dose response to pregnenolone sulfate (PS) activation. Trpm3-mutant lenses shared the downregulation of genes involved in insulin/peptide secretion and the upregulation of genes involved in Ca2+ dynamics. By contrast, Trpm3-deficient lenses did not replicate the pathophysiological changes observed in Trpm3-mutant lenses. Collectively, our data suggest that a cataract-causing substitution in the TRPM3 cation channel elicits a deleterious gain-of-function rather than a loss-of-function mechanism in the lens. Full article
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18 pages, 9873 KiB  
Article
The Roles and Regulatory Mechanisms of Tight Junction Protein Cingulin and Transcription Factor Forkhead Box Protein O1 in Human Lung Adenocarcinoma A549 Cells and Normal Lung Epithelial Cells
by Daichi Ishii, Yuma Shindo, Wataru Arai, Takumi Konno, Takayuki Kohno, Kazuya Honda, Masahiro Miyajima, Atsushi Watanabe and Takashi Kojima
Int. J. Mol. Sci. 2024, 25(3), 1411; https://doi.org/10.3390/ijms25031411 - 24 Jan 2024
Cited by 3 | Viewed by 2441
Abstract
Tight junction (TJ) protein cingulin (CGN) and transcription factor forkhead box protein O1 (FOXO1) contribute to the development of various cancers. Histone deacetylase (HDAC) inhibitors have a potential therapeutic role for some cancers. HDAC inhibitors affect the expression of both CGN and FOXO1. [...] Read more.
Tight junction (TJ) protein cingulin (CGN) and transcription factor forkhead box protein O1 (FOXO1) contribute to the development of various cancers. Histone deacetylase (HDAC) inhibitors have a potential therapeutic role for some cancers. HDAC inhibitors affect the expression of both CGN and FOXO1. However, the roles and regulatory mechanisms of CGN and FOXO1 are unknown in non-small cell lung cancer (NSCLC) and normal human lung epithelial (HLE) cells. In the present study, to investigate the effects of CGN and FOXO1 on the malignancy of NSCLC, we used A549 cells as human lung adenocarcinoma and primary human lung epithelial (HLE) cells as normal lung tissues and performed the knockdown of CGN and FOXO1 by siRNAs. Furthermore, to investigate the detailed mechanisms in the antitumor effects of HDAC inhibitors for NSCLC via CGN and FOXO1, A549 cells and HLE cells were treated with the HDAC inhibitors trichostatin A (TSA) and Quisinostat (JNJ-2648158). In A549 cells, the knockdown of CGN increased bicellular TJ protein claudin-2 (CLDN-2) via mitogen-activated protein kinase/adenosine monophosphate-activated protein kinase (MAPK/AMPK) pathways and induced cell migration, while the knockdown of FOXO1 increased claudin-4 (CLDN-4), decreased CGN, and induced cell proliferation. The knockdown of CGN and FOXO1 induced cell metabolism in A549 cells. TSA and Quisinostat increased CGN and tricellular TJ protein angulin-1/lipolysis-stimulated lipoprotein receptor (LSR) in A549. In normal HLE cells, the knockdown of CGN and FOXO1 increased CLDN-4, while HDAC inhibitors increased CGN and CLDN-4. In conclusion, the knockdown of CGN via FOXO1 contributes to the malignancy of NSCLC. Both HDAC inhibitors, TSA and Quisinostat, may have potential for use in therapy for lung adenocarcinoma via changes in the expression of CGN and FOXO1. Full article
(This article belongs to the Special Issue Lung Cancer: From Molecular Mechanisms to Novel Therapeutics)
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22 pages, 1334 KiB  
Article
A Growth Mindset Message Leads Parents to Choose More Challenging Learning Activities
by Jing Tian, Grace Bennett-Pierre, Nadia Tavassolie, Nora S. Newcombe, Marsha Weinraub, Annemarie H. Hindman, Kristie J. Newton and Elizabeth A. Gunderson
J. Intell. 2023, 11(10), 193; https://doi.org/10.3390/jintelligence11100193 - 9 Oct 2023
Cited by 5 | Viewed by 3216
Abstract
Prior research has shown that the home learning environment (HLE) is critical in the development of spatial skills and that various parental beliefs influence the HLE. However, a comprehensive analysis of the impact of different parental beliefs on the spatial HLE remains lacking, [...] Read more.
Prior research has shown that the home learning environment (HLE) is critical in the development of spatial skills and that various parental beliefs influence the HLE. However, a comprehensive analysis of the impact of different parental beliefs on the spatial HLE remains lacking, leaving unanswered questions about which specific parental beliefs are most influential and whether inducing a growth mindset can enhance the spatial HLE. To address these gaps, we conducted an online study with parents of 3- to 5-year-olds. We found that parents’ growth mindset about their children’s ability strongly predicted the spatial HLE after controlling for parents’ motivational beliefs about their children, beliefs about their own ability, children’s age, children’s gender, and family SES. Further, reading an article about growth mindset led parents to choose more challenging spatial learning activities for their children. These findings highlight the critical role of parents’ growth mindset in the spatial HLE. Crucially, these findings demonstrate that general growth mindset messages without specific suggestions for parental practices can influence parental behavior intentions. Further, these effects were also observed in the control domain of literacy, underscoring the broad relevance of the growth mindset in the HLE. Full article
(This article belongs to the Special Issue Spatial Intelligence and Learning)
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18 pages, 1260 KiB  
Article
Organizational Health Literacy as a Tool for Health Equity: Application in a High-Risk Infant Follow-Up Program
by Lindsay E. Rosenfeld, Kelly McCullagh, Carolyn J. King, Micaela Torres and Jonathan S. Litt
Children 2023, 10(10), 1658; https://doi.org/10.3390/children10101658 - 6 Oct 2023
Cited by 7 | Viewed by 2568
Abstract
Background: Healthy People 2030 emphasizes personal health literacy (individual skills) and organizational health literacy—the degree to which organizations equitably enable individuals to find, understand, and use information and services to inform health-related decisions and actions for themselves and others. However, research on the [...] Read more.
Background: Healthy People 2030 emphasizes personal health literacy (individual skills) and organizational health literacy—the degree to which organizations equitably enable individuals to find, understand, and use information and services to inform health-related decisions and actions for themselves and others. However, research on the latter is in the early stages. Methods: This study describes an organizational health literacy assessment in a U.S. urban academic children’s hospital. A variety of evidence-based health literacy assessments were used to assess patient information materials and the environment, including institutional practices, navigation, culture and language, and communication. Trained interviewers and analysts reached consensus for all assessments. Results: Information Items: SMOG scores (n = 9) ranged from 7th- to 14th-grade reading level (average = 11.3). PEMAT-P scores (n = 9) ranged from 43.8% to 93.8% understandability and 0% to 80% actionability. CDC CCI scores (literacy demand) (n = 6) ranged from 18.2% to 58.8% (≥90% = excellent). SAM scores (suitability) (n = 6) fell in the “adequate” range (43.2–58.3%). The PMOSE/IKIRSCH scores (complexity) (n = 3) noted low-moderate difficulty. Apter’s Hierarchy (n = 4) revealed three numeracy domains (50% = descriptive purposes and decision-making, 100% = interpreting information). Organization-level: Walking interviews highlighted organizational facilitators and barriers related to the pre-visit and visit environments. HLE2 domain scores ranged from 52% to 68%. Conclusions: Organizational health literacy demands far outweigh the average literacy skills of adults in the U.S. (patients and staff). Findings can be used to hone quality improvement and other processes to focus on structural solutions to increase health equity. Full article
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26 pages, 17110 KiB  
Article
Dynamic Interplay in Tumor Ecosystems: Communication between Hepatoma Cells and Fibroblasts
by Gábor Petővári, Gábor Tóth, Lilla Turiák, Anna L. Kiss, Krisztina Pálóczi, Anna Sebestyén, Adrián Pesti, András Kiss, Kornélia Baghy, Katalin Dezső, Tibor Füle, Péter Tátrai, Ilona Kovalszky and Andrea Reszegi
Int. J. Mol. Sci. 2023, 24(18), 13996; https://doi.org/10.3390/ijms241813996 - 12 Sep 2023
Cited by 6 | Viewed by 2685
Abstract
Tumors are intricate ecosystems where cancer cells and non-malignant stromal cells, including cancer-associated fibroblasts (CAFs), engage in complex communication. In this study, we investigated the interaction between poorly (HLE) and well-differentiated (HuH7) hepatoma cells and LX2 fibroblasts. We explored various communication channels, including [...] Read more.
Tumors are intricate ecosystems where cancer cells and non-malignant stromal cells, including cancer-associated fibroblasts (CAFs), engage in complex communication. In this study, we investigated the interaction between poorly (HLE) and well-differentiated (HuH7) hepatoma cells and LX2 fibroblasts. We explored various communication channels, including soluble factors, metabolites, extracellular vesicles (EVs), and miRNAs. Co-culture with HLE cells induced LX2 to produce higher levels of laminin β1, type IV collagen, and CD44, with pronounced syndecan-1 shedding. Conversely, in HuH7/LX2 co-culture, fibronectin, thrombospondin-1, type IV collagen, and cell surface syndecan-1 were dominant matrix components. Integrins α6β4 and α6β1 were upregulated in HLE, while α5β1 and αVβ1 were increased in HuH7. HLE-stimulated LX2 produced excess MMP-2 and 9, whereas HuH7-stimulated LX2 produced excess MMP-1. LX2 activated MAPK and Wnt signaling in hepatoma cells, and conversely, hepatoma-derived EVs upregulated MAPK and Wnt in LX2 cells. LX2-derived EVs induced over tenfold upregulation of SPOCK1/testican-1 in hepatoma EV cargo. We also identified liver cancer-specific miRNAs in hepatoma EVs, with potential implications for early diagnosis. In summary, our study reveals tumor type-dependent communication between hepatoma cells and fibroblasts, shedding light on potential implications for tumor progression. However, the clinical relevance of liver cancer-specific miRNAs requires further investigation. Full article
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20 pages, 150759 KiB  
Article
Using Haze Level Estimation in Data Cleaning for Supervised Deep Image Dehazing Models
by Cheng-Hsiung Hsieh and Ze-Yu Chen
Electronics 2023, 12(16), 3485; https://doi.org/10.3390/electronics12163485 - 17 Aug 2023
Cited by 1 | Viewed by 1307
Abstract
Recently, supervised deep learning methods have been widely used for image haze removal. These methods rely on training data that are assumed to be appropriate. However, this assumption may not always be true. We observe that some data may contain hazy ground truth [...] Read more.
Recently, supervised deep learning methods have been widely used for image haze removal. These methods rely on training data that are assumed to be appropriate. However, this assumption may not always be true. We observe that some data may contain hazy ground truth (GT) images. This can lead to supervised deep image dehazing (SDID) models learning inappropriate mapping between hazy images and GT images, which negatively affects the dehazing performance. To address this problem, two difficulties must be solved. One is to estimate the haze level in an image, and the other is to develop a haze level indicator to discriminate clear and hazy images. To this end, we proposed a haze level estimation (HLE) scheme based on dark channel prior and a haze level indicator accordingly for training data cleaning, i.e., to exclude image pairs with hazy GT images in the data set. With the data cleaning by the HLE, we introduced an SDID framework to avoid inappropriate learning and thus improve the dehazing performance. To verify the framework, using the RESIDE data set, experiments were conducted with three types of SDID models, i.e., GCAN, REFN and cGAN. The results show that our method can significantly improve the dehazing performance of the three SDID models. Subjectively, the proposed method generally provides better visual quality. Objectively, our method, using fewer training image pairs, was capable of improving PSNR in the GCAN, REFN, and cGAN models by 3.10 dB, 5.74 dB, and 6.44 dB, respectively. Furthermore, our method was evaluated using a real-world data set, KeDeMa. The results indicate that the better visual quality of the dehazed images is generally for models with the proposed data cleaning scheme. The results demonstrate that the proposed method effectively and efficiently enhances the dehazing performance in the given examples. The practical significance of this research is to provide an easy but effective way, that is, the proposed data cleaning scheme, to improve the performance of SDID models. Full article
(This article belongs to the Special Issue Convolutional Neural Networks and Vision Applications, 3rd Edition)
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16 pages, 825 KiB  
Article
Bilingual Home Literacy Experiences and Early Biliteracy Development among Chinese–Canadian First Graders
by Guofang Li, Fubiao Zhen, Zhen Lin and Lee Gunderson
Educ. Sci. 2023, 13(8), 808; https://doi.org/10.3390/educsci13080808 - 6 Aug 2023
Cited by 8 | Viewed by 2831
Abstract
This study was designed to examine the role of early bilingual home literacy experiences (HLE) (including parent–child shared reading, parents’ direct teaching in Chinese and English, the availability of books in both languages, and children’s access to digital devices for bilingual learning) in [...] Read more.
This study was designed to examine the role of early bilingual home literacy experiences (HLE) (including parent–child shared reading, parents’ direct teaching in Chinese and English, the availability of books in both languages, and children’s access to digital devices for bilingual learning) in the biliteracy development of 66 Chinese–Canadian first graders during the COVID-19 pandemic. Descriptive analyses reveal that overall, parents report higher engagement in English than in Chinese across the four HLE measures. Parent’s engagement in bilingual HLE differs by gender, SES, and immigration status. Pearson correlational analyses of English reading, decoding, and bilingual oral receptive vocabulary reveal that the four dimensions of HLE are not strongly related to English early literacy skills but are positively related to Chinese receptive vocabulary. Finally, hierarchical regression analyses indicate that the availability of books in Chinese and parent–child shared reading in Chinese are key factors associated with Chinese receptive vocabulary score variance; the amount of time using digital devices is found to be significantly related to English reading comprehension, but not Chinese vocabulary; and parents’ direct teaching is not significant with either English early literacy skills or Chinese receptive vocabulary. These findings have important implications for parental engagement in early bilingual home literacy activities and early literacy instruction in school. Full article
13 pages, 14505 KiB  
Article
Role of Chondroitin Sulfate Proteoglycan 5 in Steroid-Induced Cataract
by Woong-Sun Yoo, Hyemin Seong, Chieun Song, Mee-Young Choi, Bina Lee, Youngsub Eom, Hae-Jin Kim, Seung Pil Yun and Seong-Jae Kim
Cells 2023, 12(13), 1705; https://doi.org/10.3390/cells12131705 - 23 Jun 2023
Cited by 2 | Viewed by 2669
Abstract
Steroid-induced cataracts (SIC) are defined as cataracts associated with the administration of corticosteroids. Long-term glucocorticoid treatment for inflammatory diseases reportedly increases the risk of SIC, and steroids can induce cataracts by disrupting ocular growth factor balance or homeostasis. In this study, we verified [...] Read more.
Steroid-induced cataracts (SIC) are defined as cataracts associated with the administration of corticosteroids. Long-term glucocorticoid treatment for inflammatory diseases reportedly increases the risk of SIC, and steroids can induce cataracts by disrupting ocular growth factor balance or homeostasis. In this study, we verified the effect of chondroitin sulfate proteoglycan 5 (CSPG5) using dexamethasone (dexa)-treated human lens epithelial (HLE-B3) cells and the lens epithelium from the anterior capsule of SIC patients obtained during cataract surgery. CSPG5 expression increased in the lens epithelium of SIC patients. The downregulation of CSPG5 suppressed the dexa-induced epithelial–mesenchymal transition (EMT)-related protein expression and motility in HLE-B3 cells. The disruption of the transcription factors EZH2 and B-Myb downregulated CSPG5, dexa-induced fibronectin expression, and cell migration in HLE-B3 cells, reaffirming that CSPG5 expression regulates EMT in lens epithelial cells. Taken together, these results indicate that the steroid-induced effects on lens epithelial cells are mediated via alterations in CSPG5 expression. Therefore, our study emphasizes the potential of CSPG5 as a therapeutic target for the prevention and treatment of SIC. Full article
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16 pages, 4519 KiB  
Article
Glycolysis Aids in Human Lens Epithelial Cells’ Adaptation to Hypoxia
by Yuxin Huang, Xiyuan Ping, Yilei Cui, Hao Yang, Jing Bao, Qichuan Yin, Hailaiti Ailifeire and Xingchao Shentu
Antioxidants 2023, 12(6), 1304; https://doi.org/10.3390/antiox12061304 - 19 Jun 2023
Cited by 5 | Viewed by 2438
Abstract
Hypoxic environments are known to trigger pathological damage in multiple cellular subtypes. Interestingly, the lens is a naturally hypoxic tissue, with glycolysis serving as its main source of energy. Hypoxia is essential for maintaining the long-term transparency of the lens in addition to [...] Read more.
Hypoxic environments are known to trigger pathological damage in multiple cellular subtypes. Interestingly, the lens is a naturally hypoxic tissue, with glycolysis serving as its main source of energy. Hypoxia is essential for maintaining the long-term transparency of the lens in addition to avoiding nuclear cataracts. Herein, we explore the complex mechanisms by which lens epithelial cells adapt to hypoxic conditions while maintaining their normal growth and metabolic activity. Our data show that the glycolysis pathway is significantly upregulated during human lens epithelial (HLE) cells exposure to hypoxia. The inhibition of glycolysis under hypoxic conditions incited endoplasmic reticulum (ER) stress and reactive oxygen species (ROS) production in HLE cells, leading to cellular apoptosis. After ATP was replenished, the damage to the cells was not completely recovered, and ER stress, ROS production, and cell apoptosis still occurred. These results suggest that glycolysis not only performs energy metabolism in the process of HLE cells adapting to hypoxia, but also helps them continuously resist cell apoptosis caused by ER stress and ROS production. Furthermore, our proteomic atlas provides possible rescue mechanisms for cellular damage caused by hypoxia. Full article
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