Search Results (9)

Gummy stem blight (GSB), caused by Stagonosporopsis cucurbitacearum (Didymella bryoniae), poses a growing threat to greenhouse melon production. Despite this, the defense mechanism of melon against S. cucurbitacearum remains poorly understood. In this study, by employing electron microscopy and transcriptome sequencing, we investigated the cellular ultrastructure differences and gene expression dynamics of two melon accessions, PI 420145 (resistant to GSB) and ‘Baipicui’ (susceptible), pre- and post- inoculation. Our results revealed that PI 420145 exhibits a thicker waxy layer on the leaf surface and limited conidial germination without obvious signs of cell damage compared to ‘Baipicui’. The transcriptomic analysis identified a total of 23,078 differentially expressed genes (DEGs) and 974 differentially expressed long noncoding RNAs (DELs). Specifically, phenylpropanoid biosynthesis, cyanoamino acid metabolism, and MAPK signaling–plant and plant–pathogen interactions were enriched in PI 420145, while ‘Baipicui’ displayed enrichment in metabolism and autophagy. Additionally, through lncRNA–mRNA network construction, we identified a total of 38 lncRNA–mRNA targeted regulatory relationships in the four most significant KEGG pathways for disease resistance. Full article

Microbial agents such as the Bacillus species are recognized for their role as biocontrol agents against various phytopathogens through the production of diverse bioactive compounds. This study evaluates the effectiveness of Bacillus subtilis PE7 in inhibiting the growth of Didymella bryoniae, the pathogen responsible for gummy stem blight (GSB) in cucurbits. Dual culture assays demonstrate significant antifungal activity of strain PE7 against D. bryoniae. Volatile organic compounds (VOCs) produced by strain PE7 effectively impede mycelial formation in D. bryoniae, resulting in a high inhibition rate. Light microscopy revealed that D. bryoniae hyphae exposed to VOCs exhibited abnormal morphology, including swelling and excessive branching. Supplementing a potato dextrose agar (PDA) medium with a 30% B. subtilis PE7 culture filtrate significantly decreased mycelial growth. Moreover, combining a 30% culture filtrate with half the recommended concentration of a chemical fungicide yielded a more potent antifungal effect than using the full fungicide concentration alone, inducing dense mycelial formation and irregular hyphal morphology in D. bryoniae. Strain PE7 was highly resilient and was able to survive in fungicide solutions. Additionally, B. subtilis PE7 enhanced the nutrient content, growth, and development of melon plants while mitigating the severity of GSB compared to fungicide and fertilizer treatments. These findings highlight B. subtilis PE7 as a promising biocontrol candidate for integrated disease management in crop production. Full article

Infection biology and defence responses of watermelon attacked by Stagonosporopsis cucurbitacearum (syn. Didymella bryoniae) were studied in two genotypes, accessions PI189225 (moderately resistant) and 232-0125/B (susceptible). On intact leaf surfaces, spores started to germinate 14 h after inoculation (hai) with one to three germ tubes, which subsequently developed and formed appressoria. Invasion of the host tissue started at 20 hai by direct penetration from appressoria or occasionally indirectly through stomata. In the susceptible accession, a significantly higher number of direct penetrations were observed than in the moderately resistant. After invasion, hyphal colonisation was restricted in the intercellular spaces in the moderately resistant accession, whereas they developed extensively, causing tissue decay, in the susceptible accession. Macroscopic symptoms were seen in leaves of the moderately resistant accession as small and dry lesions, whereas big, water-soaked lesions developed on the susceptible accession within 48 hai. Investigations of the defence responses of the two accessions showed accumulation of H₂O₂ at penetration sites beneath appressoria in the moderately resistant, but to a lesser extent in the susceptible accession. Such H₂O₂ accumulation correlated with a reduction in penetration frequency and a lower level of hyphal growth after infection in the moderately resistant accession. There was a rapid and early increase in total peroxidase as well as β-1,3-glucanase activity in the moderately resistant compared to the susceptible accession. These results indicate that fungal penetration and development in watermelon are inhibited by a consorted action of different responses including accumulation of H₂O₂, peroxidase and β-1,3-glucanase. Full article

Fusarium oxysporum f. sp. niveum (Fon) is a devastating soil-borne fungus causing Fusarium wilt in watermelon. The present study investigated the biochemical mechanism underlying the antifungal activity exhibited by the antagonistic bacterial strain DHA41, particularly against Fon. Molecular characterization based on the 16S rRNA gene confirmed that DHA41 is a strain of Bacillus subtilis, capable of synthesizing antifungal lipopeptides, such as iturins and fengycins, which was further confirmed by detecting corresponding lipopeptide biosynthesis genes, namely ItuB, ItuD, and FenD. The cell-free culture filtrate and extracellular lipopeptide extract of B. subtilis DHA41 demonstrated significant inhibitory effects on the mycelial growth of Fon, Didymella bryoniae, Sclerotinia sclerotiorum, Fusarium graminearum, and Rhizoctonia solani. The lipopeptide extract showed emulsification activity and inhibited Fon mycelial growth by 86.4% at 100 µg/mL. Transmission electron microscope observations confirmed that the lipopeptide extract disrupted Fon cellular integrity. Furthermore, B. subtilis DHA41 emitted volatile organic compounds (VOCs) that exhibited antifungal activity against Fon, D. bryoniae, S. sclerotiorum, and F. graminearum. These findings provide evidence that B. subtilis DHA41 possesses broad-spectrum antifungal activity against different fungi pathogens, including Fon, through the production of extracellular lipopeptides and VOCs. Full article

Fusarium wilt caused by Fusarium oxysporum f. sp. niveum is one of the most devastating fungal diseases affecting watermelon (Citrullus lanatus L.). The present study aimed to identify potent antagonistic bacterial strains with substantial antifungal activity against F. oxysporum f. sp. niveum and to explore their potential for biocontrol of Fusarium wilt in watermelon. Out of 77 isolates from watermelon rhizosphere, six bacterial strains—namely, DHA4, DHA6, DHA10, DHA12, DHA41, and DHA55—exhibited significant antifungal activity against F. oxysporum f. sp. niveum, as well as other phytopathogenic fungi, including Didymella bryoniae, Sclerotinia sclerotiorum, Fusarium graminearum, and Rhizoctonia solani. These Gram-positive, rod-shaped, antagonistic bacterial strains were able to produce exo-enzymes (e.g., catalase, protease, and cellulase), siderophore, and indole-3-acetic acid and had the ability to solubilize phosphate. In greenhouse experiments, these antagonistic bacterial strains not only promoted plant growth but also suppressed Fusarium wilt in watermelon. Among these strains, DHA55 was the most effective, achieving the highest disease suppression of 74.9%. Strain DHA55 was identified as Bacillus amyloliquefaciens based on physiological, biochemical, and molecular characterization. B. amyloliquefaciens DHA55 produced various antifungal lipopeptides, including iturin, surfactin, and fengycin, that showed significant antifungal activities against F. oxysporum f. sp. niveum. Microscopic observations revealed that B. amyloliquefaciens DHA55 exhibited an inhibitory effect against F. oxysporum f. sp. niveum on the root surface of watermelon plants. These results demonstrate that B. amyloliquefaciens DHA55 can effectively promote plant growth and suppress the development of watermelon Fusarium wilt, providing a promising agent for the biocontrol of Fusarium wilt in watermelon. Full article

Mediterranean greenhouses are usually covered by plastic materials (films); these films allow light to pass through them, modifying some of their characteristics. The properties of the plastic cover influence the development of greenhouse crops. In addition, it can influence the stresses that the plants endure and the development of fungal diseases in the crop. The aim of this study is to analyze the effect that an experimental film cover, with high transmittance and high light diffusivity, produces on the development of fungal diseases on a cucumber crop (Cucumis sativus L.). Two different film covers were compared: (i) commercial film (transmittance of 85%; diffusivity of 60%); and (ii) experimental film (transmittance of 90%; diffusivity of 55%). The study was carried out across two autumn–winter crop cycles in a multi-span greenhouse divided into two isolated sectors. Three fungal diseases caused the main damage to the cucumber crop: downy mildew (Pseudoperonospora cubensis), powdery mildew (Sphaerotheca fuliginia) and gummy stem blight (Didymella bryoniae). In the case of powdery mildew, a greater severity in the sector was observed with the commercial film in comparison with the sector with the experimental film, with significant statistical differences between the two sectors in both crop cycles. Downy mildew and gummy stem blight were fungal diseases with less presence than downy mildew, and a greater presence of these two fungal diseases in the sector with the commercial film was also observed in both crop cycles. Full article

Gummy stem blight (GSB) caused by Didymella bryoniae (D. bryoniae) is a worldwide fungal soil-borne disease that can cause severe yield reduction of watermelon. To shorten the monitoring time of carbendazim-resistant strains of D. bryoniae in the field, in this study, we developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection of carbendazim-resistant strains of D. bryoniae. The β-tubulin gene of carbendazim-resistant strains was selected as the target for primer design. Based on the color change of hydroxy naphthol blue (HNB) and gel electrophoresis, the optimal reaction conditions for LAMP were determined at 65 °C for 50 min. In specificity tests, the LAMP assay was able to distinguish between carbendazim-resistant and sensitive strains of D. bryoniae. Moreover, in sensitivity tests, the detection limit was 1 ng/μL D. bryoniae DNA of the carbendazim-resistant strain. In addition, the LAMP method was successfully applied to detect carbendazim-resistant strains in D. bryoniae-infested samples. Therefore, the developed LAMP assay provides a new method for the rapid detection of carbendazim-resistant strains of D. bryoniae. Full article

Cucurbits are an important vegetable crop of the gourd family. Unfortunately, gummy stem blight (GSB) causes a major fungal disease on Cucurbitaceous vegetable crops. It is also known as black root when affecting fruits, and it is found all over the world. GSB is caused by the fungal pathogen Didymella bryoniae. Research efforts have investigated the different developmental stages and various parts of Cucurbits affected with this disease. In the present paper, we have completed a systematic review for the disease’s symptomatic, pathogenic microbes, resistance resources, resistance inheritance regularity, molecular biology and genomic study of resistance gene and control method, etc., on Cucurbits. This review provides the background and rationale for future studies aiming to address the issues existing in gummy stem blight research and development. Full article

Watermelon (Citrullus lanatus) is a nutritionally rich and economically important horticultural crop of the Cucurbitaceae family. Gummy stem blight (GSB) is a major disease of watermelon, which is caused by the fungus Didymella bryoniae, and results in substantial economic losses in terms of yield and quality. However, only a few molecular studies have focused on GSB resistance in watermelon. Nucleotide binding site (NBS)-encoding resistance (R) genes play important roles in plant defense responses to several pathogens, but little is known about the role of NBS-encoding genes in disease resistance in watermelon. The analyzed NBS-encoding R genes comprises several domains, including Toll/interleukin-1 receptor(TIR), NBS, leucine-rich repeat (LRR), resistance to powdery mildew8(RPW8) and coiled coil (CC), which are known to be involved in disease resistance. We determined the expression patterns of these R genes in resistant and susceptible watermelon lines at different time points after D. bryoniae infection by quantitative RT-PCR. The R genes exhibited various expression patterns in the resistant watermelon compared to the susceptible watermelon. Only six R genes exhibited consistent expression patterns (Cla001821, Cla019863, Cla020705, Cla012430, Cla012433 and Cla012439), which were higher in the resistant line compared to the susceptible line. Our study provides fundamental insights into the NBS-LRR gene family in watermelon in response to D. bryoniae infection. Further functional studies of these six candidate resistance genes should help to advance breeding programs aimed at improving disease resistance in watermelons. Full article