Search Results (19)

Quillay (Quillaja saponaria Molina) and peumo (Cryptocarya alba [Molina] Looser) are two tree species endemic to Chile that grow in Mediterranean climate zones, characterized by a summer season with high temperatures, high solar radiation, and low soil water availability. A study was conducted with 2-year-old Q. saponaria and C. alba plants and two substrate water conditions: well-watered and controlled water restriction. At the end of the study, anatomical leaf modifications were analyzed. The tissues were anatomically described in transverse sections of juvenile and adult leaves, measuring leaf thickness, cuticle thickness, and cell density of the mesophyll parenchymal tissues. In the young leaves of Q. saponaria plants undergoing water restriction treatment, an increase in cuticle and leaf thickness and a decrease in the density of the palisade and spongy parenchyma were observed. In contrast, a significant reduction in leaf thickness was observed in adult leaves of both species with water restriction treatment. The anatomical changes in the leaves of Q. saponaria and C. alba suggest an adaptation to adverse environmental conditions, such as water restriction. Full article

The traditional knowledge of sunggau rafters for Apis dorsata nesting is essential for livelihood and forest conservation. We determine the plant species needed to support the conservation of A. dorsata in Belitung Islands, Indonesia, by investigating the distribution of the sunggau in the three types of forests, the traditional knowledge regarding the plant species used for sunggau, and the vegetation structure of forests in which the sunggau is installed. Distribution of sunggau were explored on heath, swamp heath, and mangrove forests. We conducted a vegetation survey in those forests and an ethnobotanical survey by interviewing bee farmers regarding the plants used for sunggau and other uses. We found 95 sunggau distributed in the heath, swamp heath, and mangrove forest. Based on interviews, we recorded 65 plant species for sunggau and six other uses, including bee forages. Calophyllum sp., Cryptocarya sp., Melaleuca cajuputi, and Syzygium urceolatum are the most important plants according to bee farmers. The last two species dominate all forests, except mangroves, which are dominated by Lumnitzera littorea, according to vegetation surveys. However, the availability of several plants for sunggau is declining. Therefore, the conservation of A. dorsata needs the sustainability of sunggau and bee forage plants. Full article

Three new Aceria species from South China are described and illustrated. Aceria bischofiaesp. nov. was collected on Bischofia javanica Blume (Phyllanthaceae), inducing galls on surfaces of the leaves; Aceria cryptocaryaesp. nov. was collected on Cryptocarya metcalfiana Allen (Lauraceae), causing the formation of erinea on the undersurface of the leaves; and Aceria buddlejaesp. nov. was collected as a vagrant on Buddleja lindleyana Fort. (Scrophulariaceae) leaves, and no symptoms were observed on the host plant. Full article

The allelophatic effect of the invasive Fabaceae, Ulex europaeus and Teline monspessulana, on the production of phenolic compounds in C. alba seedlings was investigated. It was expected that the oxidative stress caused by the allelochemicals released by both invaders would induce a differential response in the production of phenolic compounds in C. alba seedlings. These antioxidant mechanisms guaranteed C. alba plants’ survival, even to the detriment of their initial growth. Cryptocarya alba seedlings were irrigated with T. monspessulana (TE) and U. europaeus (UE) extracts and water as a control. After eight months, morphometric variables were evaluated, and leaves were collected for histochemical analysis. The methanol extracts from treatments and control leaves were used for anthocyanin, phenol, and antioxidant activity quantifications. Both invasive species induced an inhibitory effect on the morphometric variables. Teline monspessulana induced leaf damage and increased the anthocyanin content by 4.9-fold, but did not affect the phenol content. Ulex europaeus induces root damage and a decrease in phenol content, but does not affect the anthocyanin content. Both Fabaceae extracts affected the profile and polyphenol concentration and consequently decreased the antioxidant capacity of C. alba leaves at low extract concentrations. Phenols, lignin, and ROS accumulate on C. alba leaves, but the histochemical reactions were less intense under UE. Although C. alba develops different antioxidant protection mechanisms against stress induced by UE and TE, its survival is guaranteed, even to the detriment of its initial growth. Full article

Climate change is threatening the restoration efforts in Mediterranean ecosystems, and there is still little knowledge about the responses of some sclerophyllous species to plant management techniques. This study assessed the effects of the planting date, use of mulch, and controlling light levels on the early survival and growth of sclerophyllous species established in island plots. A factorial design was installed in the Metropolitan Region of central Chile, with a treatment combination that included three planting dates (January: midsummer, April: autumn, and June: winter), three light levels (full sun exposed (T0), shaded at 35% (T35), and shaded at 70% (T70)), and two mulch levels (no mulch versus mulch application). Additionally, we tested the species effects within the island plot. We measured survival, as well as plant diameter and height increments, 1 year after establishment. Each island plot contained three seedlings of Acacia caven Mol., two of Quillaja saponaria Mol., two of Maytenus boaria Mol., one of Schinus polygamus (Cav.) Cabrera, and one of Cryptocarya alba (Mol.) Losser. We found a significant main effect for all the factors assessed. Despite the high mortality in the trial, survival was increased around fourfold by planting in winter, using mulch, or using either of the shading levels. At the species level, average survival ranged from 7% for C. alba to 27% for S. polygamous. To increase plant survival in the restoration of this ecosystem, it is necessary to intensify the management techniques and the use of these types of eco-technologies; if not, the restoration may fail. Full article

The Chilean matorral is a heavily threatened Mediterranean-type ecosystem due to human-related activities such as anthropogenic fires. Mycorrhizal fungi may be the key microorganisms to help plants cope with environmental stress and improve the restoration of degraded ecosystems. However, the application of mycorrhizal fungi in the restoration of the Chilean matorral is limited because of insufficient local information. Consequently, we assessed the effect of mycorrhizal inoculation on the survival and photosynthesis at set intervals for two years after a fire event in four native woody plant species, namely: Peumus boldus, Quillaja saponaria, Cryptocarya alba, and Kageneckia oblonga, all dominant species of the matorral. Additionally, we assessed the enzymatic activity of three enzymes and macronutrient in the soil in mycorrhizal and non-mycorrhizal plants. The results showed that mycorrhizal inoculation increased survival in all studied species after a fire and increased photosynthesis in all, but not in P. boldus. Additionally, the soil associated with mycorrhizal plants had higher enzymatic activity and macronutrient levels in all species except in Q. saponaria, in which there was no significant mycorrhization effect. The results suggest that mycorrhizal fungi could increase the fitness of plants used in restoration initiatives after severe disturbances such as fires and, consequently, should be considered for restoration programs of native species in threatened Mediterranean ecosystems. Full article

Cryptoconcatones A-L represent a series of 12 dihydropyrone derivatives isolated from the evergreen tree Cryptocarya concinna Hance, which is well distributed in southeast Asia. The lead compound in the series, cryptoconcatone L, has revealed antiproliferative activity against cultured cancer cells but its mechanism of action remains unknown. Based on a structural analogy with the anticancer natural product pironetin, which is well known for binding covalently to α-tubulin and for functioning as a microtubule polymerization inhibitor, we investigated the interaction of cryptoconcatones with tubulin dimers using molecular docking. The α-tubulin binding capacity of each compound was quantified (through calculation of the empirical energy of interaction ΔE) and structure–binding relationships were delineated. Two compounds were found to interact with α-tubulin much more potently than pironetin: cryptoconcatones F and L. In both cases, the facile formation of a covalent bond with Cys316 was evidenced, as observed with the parent compound pironetin. A few other pironetin analogues were investigated, including spicigerolide, which is an analogue of another known α-tubulin binder. Altogether, this study points to the identification of a series of 5,6-dihydro-α-pyrones as α-tubulin-binding agents. The study contributes to a better understanding of the mechanism of action of cryptoconcatones and should help the design of analogues targeting the pironetin site of α-tubulin. Full article

Plant functional traits are rarely used in tree species classification, and the impact of vertical canopy positions on collecting samples for classification also remains unclear. We aim to explore the feasibility and effectiveness of leaf traits in classification, as well as to detect the effect of vertical position on classification accuracy. This work will deepen our understanding of the ecological mechanism of natural forest structure and succession from new perspectives. In this study, we collected foliar samples from three canopy layers (upper, middle and lower) and measured their spectra, as well as eight well-known leaf traits. We used a leaf hyperspectral reflectance (LHR) dataset, leaf functional traits (LFT) dataset and LFT + LHR dataset to classify six dominant tree species in a subtropical evergreen broad-leaved forest. Our results showed that the LFT + LHR dataset achieved the highest classification results (overall accuracy (OA) = 77.65% and Kappa = 0.73), followed by the LFT dataset (OA = 74.26% and Kappa = 0.69) and the LHR dataset (OA = 69.06% and Kappa = 0.63). Along the vertical canopy, the OA and Kappa increased from the lower to the upper layers, and the combination data of the three canopy layers achieved the highest accuracy. For the individual tree species, the shade-tolerant species (including Machilus chinensis, Cryptocarya chinensis and Cryptocarya concinna) produced higher accuracies than the light-demanding species (including Schima superba and Castanopsis chinensis). Our results provide an approach for enhancing tree species recognition from the plant physiology and biochemistry perspective and emphasize the importance of vertical direction in forest community research. Full article

Sitophilus zeamais is a primary pest of maize. Our aim was to perform a qualitative review and meta-analyses with 56 scientific articles published from 1 January 2000 to 1 October 2022 dealing with direct (topical application) and indirect (impregnation of essential oils, EOs, onto filter paper or maize grains) contact toxicity of EOs against S. zeamais. Three independent meta-analyses of single means of LD₅₀ (direct contact) and LC₅₀ (indirect contact) were conducted using a random effect model. Essential oils more frequently evaluated were those belonging to Asteraceae, Apiaceae, Lamiaceae, Myrtaceae, Piperaceae, and Rutaceae. The LC₅₀ global mean values were 33.19 µg/insect (CI₉₅ 29.81–36.95) for topical application; 0.40 µL/cm² (CI₉₅ 0.25–0.65) for filter paper indirect contact; and 0.50 µL/g maize (CI₉₅ 0.27–0.90) for maize grains indirect contact. The species Carum carvi, Salvia umbratica, Ilicium difengpi, Periploca sepium, Cephalotaxus sinensis, Murraya exotica, Rhododendron anthopogonoides, Ruta graveolens, Eucalyptus viminalis, Ocotea odorifera, Eucalyptus globulus, Eucalyptus dunnii, Anethum graveolens, Ilicium verum, Cryptocarya alba, Azadirachta indica, Chenopodium ambrosioides, Cupressus semperivens, Schinus molle, Piper hispidinervum, Mentha longifolia, and Croton pulegiodorus showed LC₅₀ or LD₅₀ values lower than the global means, indicating good insecticidal properties. Our results showed that EOs have great potential to be used as bioinsecticides against S. zeamais. Full article

As a consequence of the megadrought in Central Chile, it is expected that most of the distribution of woody species will be narrowed in the northern limits because of restrictions imposed by soil matric potential on seed germination. In this study, we analyzed the effect of the soil matric potential on seed germination and initial recruitment of the sclerophyllous species Prosopis chilensis, Quillaja saponaria and Cryptocarya alba from contrasting geographic origins (i.e., seed sources). We evaluated the germination capacity (%) under different matric potentials (i.e., 0, −6, −33, −750 and −1250 kPa) for 100 days. Soil matric potential of −1250 kPa negatively affected the germination capacity of the three species. P. chilensis seeds stopped germinating under soil matric potential close to −1200 kPa, whereas in Q. saponaria and C. alba the complete inhibition of germination was under −1000 kPa. Seed sources also differed in their germination capacity by soil matric potential: northern seed sources of P. chilensis germinated with the lowest soil matric potential. There was no clear trend in Q. saponaria and C. alba, but in general, southern seed sources performed better than the northern ones. The results showed that Ѱ_m in the soil played an important role in the germinative capacity against different seed source origins, but not in soils with a north–south gradient. Full article

Natural regeneration of recalcitrant-seeded tree species is strongly limited in Mediterranean-type climate zones due to increasing droughts imposed by climate change. Direct seeding can be a low-cost alternative to seedling establishment, but there is still limited information for some species. This study aimed to assess the effects of the seed source and forest cover on the germination and survival of the endemic Cryptocarya alba Mol. established through direct seeding. Three habitat types differing in forest cover were identified within the natural park Reserva Natural Altos de Cantillana, Metropolitan Region, Chile. The forest cover corresponded to open (canopy density <25%), semi-dense (canopy density around 50%), and dense forest (canopy density >75%). All forest cover had C. alba as one of the dominant species. At each habitat type, 38 families from four seed sources (Cuesta La Dormida (CD), Antumapu (AN), Cantillana (CA, local seed source) and Cayumanque (CY)) were directly seeded. Germination (Germin) and survival (Surv) were evaluated weekly during one growing season. There were significant differences between seed sources in Germin and Surv, with means values varying from 7.8% to 37% for Germin and 0% to 20% for Surv. The local seed source CA had the highest values in both traits. A significant variation was also observed between families within seed sources only for Germin. The dense forest cover had the highest Germin (22%) and Surv (55%) results compared to the other forest cover types, which was partially associated with differences in soil moisture, temperature, and bulk density. Due to the most frequent droughts in these Mediterranean-type climate zones, the use of local seeds on dense forest cover is recommended for the direct seeding of the species in the initial recruitment. Full article

Plants growing in subtropical regions are often affected by high temperature and high light in summer and low temperature and high light in winter. However, few studies have compared the photoprotection mechanism of tree species at different successional stages in these two environments, although such studies would be helpful in understanding the succession of forest communities in subtropical forests. In order to explore the strategies used by dominant species at different successional stages to cope with these two environmental conditions, we selected two dominant species in the mid-successional stage, Schima superba and Castanopsis chinensis, and two dominant species in the late-successional stage, Machilus chinensis and Cryptocarya chinensis. The cell membrane permeability, chlorophyll fluorescence, chlorophyll content, and a few light-protective substances of these dominant species were measured in summer and winter. The results show that in summer, the young leaves of dominant species in the mid-successional stage showed higher anthocyanin content and superoxide dismutase (SOD) activity, while those in the late-successional stage showed higher flavonoid and total phenolic content, total antioxidant activity, non-photochemical quenching (NPQ), and carotenoid/chlorophyll (Car/Chl) ratio. In winter, young leaves of dominant species in the mid-successional stage were superior to those in the late-successional stage only in terms of catalase (CAT) activity and NPQ, while the anthocyanin, flavonoids, and total phenol content, total antioxidant capacity, and Car/Chl ratio were significantly lower compared to the late-successional stage. Our results show that the dominant species in different successional stages adapted to environmental changes in different seasons through the alterations in their photoprotection strategies. In summer, the dominant species in the mid-successional stage mainly achieved photoprotection through light shielding and reactive-oxygen-species scavenging by SOD, while the antioxidant capacity of trees in the late-successional stage mainly came from an increased antioxidative compounds and heat dissipation. In winter, the dominant species in the mid-successional stage maintained their photoprotective ability mainly through the scavenging of reactive oxygen species by CAT and the heat dissipation provided by NPQ, while those in the late-successional stage were mainly protected by a combination of processes, including light shielding, heat dissipation, and antioxidant effects provided by enzymatic and non-enzymatic antioxidant systems. In conclusion, our study partially explains the mechanism of community succession in subtropical forests. Full article

Cryptocaryone (CPC) is a bioactive dihydrochalcone derived from Cryptocarya plants, and its antiproliferation was rarely reported, especially for ovarian cancer (OVCA). This study aimed to examine the regulation ability and mechanism of CPC on three histotypes of OVCA cells (SKOV3, TOV-21G, and TOV-112D). In a 24 h MTS assay, CPC showed antiproliferation effects to OVCA cells, i.e., IC₅₀ values 1.5, 3, and 9.5 μM for TOV-21G, SKOV3, and TOV-112D cells. TOV-21G and SKOV3 cells showed hypersensitivity to CPC when applied for exposure time and concentration experiments. For biological processes, CPC stimulated the generation of reactive oxygen species and mitochondrial superoxide and promoted mitochondrial membrane potential dysfunction in TOV-21G and SKOV3 cells. Apoptosis was detected in OVCA cells through subG1 accumulation and annexin V staining. Apoptosis signaling such as caspase 3/7 activities, cleaved poly (ADP-ribose) polymerase, and caspase 3 expressions were upregulated by CPC. Specifically, the intrinsic and extrinsic apoptotic caspase 9 and caspase 8 were overexpressed in OVCA cells following CPC treatment. Moreover, CPC also stimulated DNA damages in terms of γH2AX expression and increased γH2AX foci. CPC also induced 8-hydroxy-2′-deoxyguanosine DNA damages. These CPC-associated principal biological processes were validated to be oxidative stress-dependent by N-acetylcysteine. In conclusion, CPC is a potential anti-OVCA natural product showing oxidative stress-dependent antiproliferation, apoptosis, and DNA damaging functions. Full article

Little attention has been paid to the impact of vertical canopy position on the leaf spectral properties of tall trees, and few studies have explored the ability of leaf spectra to characterize the variation of leaf traits across different canopy positions. Using a tower crane, we collected leaf samples from three canopy layers (lower, middle, and upper) and measured eight leaf traits (equivalent water thickness, specific leaf area, leaf carbon content, leaf nitrogen content, leaf phosphorus content, leaf chlorophyll content, flavonoid, and nitrogen balance index) in a subtropical evergreen broadleaved forest. We evaluated the variability of leaf traits and leaf spectral properties, as well as the ability of leaf spectra to track the variation of leaf traits among three canopy layers for six species within the entire reflectance spectrum. The results showed that the eight leaf traits that were moderately or highly correlated with each other showed significant differences along the vertical canopy profile. The three canopy layers of leaf spectra showed contrasting patterns for light-demanding (Castanopsis chinensis, Castanopsis fissa, Schima superba, and Machilus chinensis) and shade-tolerant species (Cryptocarya chinensis and Cryptocarya concinna) along the vertical canopy profile. The spectra at the lower and upper canopy layers were more sensitive than the middle layer for tracking the variation of leaf chlorophyll and flavonoid content. Our results revealed that it is important to choose an appropriate canopy layer for the field sampling of tall trees, and we suggest that flavonoid is an important leaf trait that can be used for mapping and monitoring plant growth with hyperspectral remote sensing. Full article

Forest fires are one of the main environmental threats in Chile. Fires in this Mediterranean climate region frequently affect native forests and exotic plantations, including in several cases urban and rural settlements. Considering the scarcity of information regarding the fire response dynamics of tree species that are frequently affected by fires, this study aims to establish a flammability classification according to the evolution of the fire initiation risk presented by the most affected forest species in the Valparaíso region. Three exotic species, Eucalyptus globulus, Pinus radiata, and Acacia dealbata, and two native species, Cryptocarya alba and Quillaja saponaria, were studied. Flammability assays indicate that E. globulus, A. dealbata, and C. alba are extremely flammable, whereas P. radiata and Q. saponaria are flammable. Furthermore, E. globulus and A. dealbata have the highest heating values while Q. saponaria has the lowest values. The extreme flammability of E. globulus, A. dealbata, and C. alba indicates a high susceptibility to ignite. Furthermore, the high heat of combustion of E. globulus and A. dealbata can be associated with a high energy release, increasing the risk of fires spreading. In contrast, Q. saponaria has the lowest predisposition to ignite and capacity to release heat. Accordingly, this work shows that all studied tree species contain organic metabolites that are potentially flammable (sesquiterpenes, aliphatic hydrocarbons, alcohol esters, ketones, diterpenes, and triterpenes) and can be considered as drivers of flammability in vegetation. Finally, these preliminary results will aid in the construction of more resilient landscapes in the near future. Full article