Search Results (23)

Monoterpene indole alkaloids (MIAs) exhibit diverse structures and pharmacological effects. Annotating MIAs in herbal medicines remains challenging when using liquid chromatography combined with high-resolution mass spectrometry (LC-HRMS). This study introduced a new annotation strategy employing LC-HRMS to efficiently identify MIAs in herbal medicines. Briefly, MS² spectra under multiple collision energies (MCEs/MS²) helped capture high-quality product ions across a range of mass-to-charge (m/z) values, revealing key MS² features such as diagnostic product ions (DPIs), characteristic cleavages (CCs), and neutral/radical losses (NLs/RLs). Next, feature-based molecular networking (FBMN) was created to map the structural relationships among MIAs across large MS datasets. Potential MIAs were then graded and annotated through systematic comparison with known biosynthetic pathways (BPs), derived skeletons, and their characteristic substituents. The MCEs/MS²-FBMN/BPs workflow was first applied to annotate MIAs in the alkaloids from the leaf of Alstonia scholaris (ALAS), a new botanical drug for respiratory diseases. A total of 229 MIAs were systematically annotated and classified, forming a solid basis for future clinical research on ALAS. This study offers an effective strategy that enhances the structural annotation of MIAs within complex herbal medicines. Full article

To support the national goals of carbon peaking and carbon neutrality, this study proposes a household carbon emission prediction model based on Gene Expression Programming (GEP) for low-carbon retrofitting of aging residential areas in arid-cold regions. Focusing on 15 typical aging communities in Kundulun District, Baotou City, a 17-dimensional dataset encompassing building characteristics, demographic structure, and energy consumption patterns was collected through field surveys. Key influencing factors (e.g., electricity usage and heating energy consumption) were selected using Pearson correlation analysis and the Random Forest (RF) algorithm. Subsequently, a hybrid prediction model was constructed, with its parameters optimized by minimizing the root mean square error (RMSE) as the fitness function. Experimental results demonstrated that the model achieved an R² value of 0.81, reducing RMSE by 77.1% compared to conventional GEP models and by 60.4% compared to BP neural networks, while significantly improving stability. By combining data dimensionality reduction with adaptive evolutionary algorithms, this model overcomes the limitations of traditional methods in capturing complex nonlinear relationships. It provides a reliable tool for precision-based low-carbon retrofits in aging residential areas of arid-cold regions and offers a methodological advance for research on building carbon emission prediction driven by urban renewal. Full article

Amid escalating global concerns over climate change and sustainable development, carbon emissions have emerged as a critical issue for the international community. The control of carbon dioxide (CO₂) emissions is particularly crucial for meeting the objectives of the Paris Agreement. This study applied the LMDI decomposition method and a BP neural network model to thoroughly analyse the factors influencing carbon emissions in Henan Province’s transportation sector and forecast future trends. Our core contribution is the development of an integrated model that quantifies the impact of key factors on carbon emissions and offers policy recommendations. This study concludes that by optimizing the energy structure and enhancing energy efficiency, China can meet its carbon peak and neutrality targets, thereby providing scientific guidance for sustainable regional development. Full article

Hydrogen energy’s economic efficiency is the key for China to obtain the goal of “carbon neutrality” by 2060. Different from the bottom-up methods and learning rate methods, this study estimates the hydrogen prices in China and typical regions by 2060 from the perspectives of economics and machine learning. The main factors influencing hydrogen price are determined from the perspectives of economics: hydrogen production, demand, and cost. A novel model is established based on combined machine learning models to predict hydrogen price. The hydrogen production is predicted based on the trained BP neural network model optimized by particle swarm optimization considering the uses of hydrogen. The hydrogen prices prediction model is built by applying a least squares support vector machine optimized by Bayesian optimization considering the hydrogen production, hydrogen demand, natural gas price, coal price, electricity price, and green hydrogen share. Moreover, the hydrogen prices in typical regions in China are compared with the average prices. The results show that the hydrogen price is estimated to decrease below CNY 12/kg and the hydrogen price in Northwest China will be lower than CNY 7.5/kg due to low electricity cost by 2060. Full article

Mitigating the rate of global warming is imperative to preserve the natural environment upon which humanity relies for survival; greenhouse gas emissions serve as the principal driver of climate change, rendering the promotion of urban carbon peaking and carbon neutrality a crucial initiative for effectively addressing climate change and attaining sustainable development. This study addresses the inherent uncertainties and complexities associated with carbon dioxide emission accounting by undertaking a scenario prediction analysis of peak carbon emissions in Dalian, utilizing the STIRPAT model in conjunction with a GA-BP neural network model optimized through a genetic algorithm. An analysis of the mechanisms underlying the influencing factors of carbon emissions, along with the identification of the carbon emission peak, is conducted based on carbon emission accounting derived from nighttime lighting data. The GA-BP prediction model exhibits significant advantages in addressing the nonlinear and non-stationary characteristics of carbon emissions, attributable to its robust mapping capabilities and probabilistic analysis proficiency. The findings reveal that energy intensity, tertiary industry value, resident population, and GDP are positively correlated with carbon emissions in Dalian, ranked in order of importance. In contrast, population density significantly reduces emissions. The GA-BP model predicts carbon emissions with 99.33% accuracy, confirming its excellent predictive capability. The recommended strategy for Dalian to achieve its carbon peak at the earliest is to adopt a low-carbon scenario, with a forecasted peak of 191.79 million tons by 2033. Full article

Burkholderia gladioli pv. cocovenenans is a serious safety issue in black fungus due to the deadly toxin, bongkrekic acid. This has triggered the demand for an efficient toxigenic phenotype recognition method. The objective of this study is to develop an efficient method for the recognition of toxin-producing B. gladioli strains. The potential of multilocus sequence typing and a back propagation neural network for the recognition of toxigenic B. cocovenenans was explored for the first time. The virulent strains were isolated from a black fungus cultivation environment in Qinba Mountain area, Shaanxi, China. A comprehensive evaluation of toxigenic capability of 26 isolates were conducted using Ultra Performance Liquid Chromatography for determination of bongkrekic acid and toxoflavin production in different culturing conditions and foods. The isolates produced bongkrekic acid in the range of 0.05–6.24 mg/L in black fungus and a highly toxin-producing strain generated 201.86 mg/L bongkrekic acid and 45.26 mg/L toxoflavin in co-cultivation with Rhizopus oryzae on PDA medium. Multilocus sequence typing phylogeny (MLST) analysis showed that housekeeping gene sequences have a certain relationship with a strain toxigenic phenotype. We developed a well-trained, back-propagation neutral network for prediction of toxigenic phenotype in B. gladioli based on MLST sequences with an accuracy of 100% in the training set and an accuracy of 86.7% in external test set strains. The BP neutral network offers a highly efficient approach to predict toxigenic phenotype of strains and contributes to hazard detection and safety surveillance. Full article

The aim of this study is to identify an alternative approach for simulating the in vitro fermentation and quantifying the production of rumen methane and rumen acetic acid during the rumen fermentation process with different total mixed rations. In this experiment, dietary nutrient compositions (neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), and dry matter (DM)) were selected as input parameters to establish three prediction models for rumen fermentation parameters (methane and acetic acid): an artificial neural network model, a genetic algorithm-bp model, and a support vector machine model. The research findings show that the three models had similar simulation results that aligned with the measured data trends (R² ≥ 0.83). Additionally, the root mean square errors (RMSEs) were ≤1.85 mL/g in the rumen methane model and ≤2.248 mmol/L in the rumen acetic acid model. Finally, this study also demonstrates the models’ capacity for generalization through an independent verification experiment, as they effectively predicted outcomes even when significant trial factors were manipulated. These results suggest that machine learning-based in vitro rumen models can serve as a valuable tool for quantifying rumen fermentation parameters, guiding the optimization of dietary structures for dairy cows, rapidly screening methane-reducing feed options, and enhancing feeding efficiency. Full article

The promotion of energy-saving household appliances is important to save energy and reduce emissions to realize peak carbon dioxide emissions and carbon neutrality. The objective of this study is to evaluate the influencing factors of energy-saving refrigerator purchases from the supply and demand sides. First, we analyze the promotional focus and attention to energy efficiency to reflect the characteristics of refrigerators that are popular with consumers in online purchases. Secondly, descriptive statistical analysis, linear regression equation, and the BP neural network model are used to analyze the current situation of consumers’ purchasing and use of energy-saving refrigerators, exploring consumers’ awareness of energy-efficiency labels and factors affecting the purchasing of energy-saving refrigerators. The results show that (1) the energy-efficiency level of consumers’ choices of refrigerators has improved with an increase in income and consumption. The Grade 1 refrigerators account for 55.26% from the supply side and 62.50% from the demand side; (2) energy-efficiency cognition and trust, environmental awareness, and economic motivation have positive effects on purchase intentions towards energy-saving refrigerators; (3) consumers will purchase energy-saving refrigerators that are more expensive but offer higher energy efficiency for the long-term total cost considering that the use cost of energy-saving refrigerators is lower. This study provides a reference to promote energy-saving refrigerators from the perspectives of enterprises, governments, and the public. Full article

In order to solve the problem of low voltage caused by unbalanced load in the distribution network, a transformer loss intelligent prediction model under unbalanced load is proposed. Firstly, the mathematical model of a transformer with an unbalanced load is established. The zero-sequence impedance and neutral line current of the transformer are calculated by using the Chaos Game Optimization algorithm (CGO), and the correctness of the mathematical model is proved by using actual data. Then, the correlation among network input variables is eliminated by using Principal Component Analysis (PCA), so the number of network input variables is decreased. At the same time, Sparrow Search Algorithm (SSA) is used to optimize the initial weight and threshold of the BP network, and an accurate transformer loss prediction model based on the PCA-SSA-BP is established. Finally, compared with the transformer loss prediction model based on BP network, Genetic Algorithm optimized BP network (GA-BP), Particle Swarm optimized BP network (PSO-BP) and Sparrow Search Algorithm optimized BP network (SSA-BP), the transformer loss prediction model based on PCA-SSA-BP network has been proven to be accurate by using actual data and it is helpful for low-voltage recovery in the distribution network. Full article

China has made remarkable achievements in reducing carbon emissions in recent years. However, there is still much reduction room before achieving carbon neutrality. In Beijing, the capital of China, it is a strategic choice to respond to global climate change by promoting green and low-carbon development. This paper calculates the carbon dioxide emissions of key industries in Beijing and analyzes the temporal evolution trend of carbon emissions. Carbon dioxide emissions in Beijing before 2030 are predicted based on the grey prediction GM (1,1) and BP neural network model. The effects of factors of carbon dioxide emissions are discussed using the threshold regression model under different economic conditions. The results show that energy consumption intensity, GDP per capita, and the ownership of civil cars have a positive impact on carbon dioxide emissions, while the number of permanent residents and urban green space areas have a negative impact on carbon dioxide emissions. These findings of carbon emission prediction and influencing factors contribute to carbon reduction path design. Related policy implications on carbon emission reduction are put forward from the aspects of promoting industrial upgrading, accelerating the construction of advanced economic structures, optimizing transportation structures, and strengthening green building development. Full article

Lumbriculus variegatus is a typical cold-water worm and is mainly distributed in the Tibetan Plateau and Northeast in China. The current study aimed to explore the genetic diversity and phylogeography of L. variegatus sampled from different geographical regions based on concatenated (COI + 16S rRNA, 879 bp) genes. Among 63 L. variegatus specimens, 29 haplotypes were identified with high haplotype diversity (h = 0.923) and nucleotide diversity (π = 0.062). The Bayesian phylogenetic analysis and Median-joining haplotype network revealed two lineages, or species, of L. variegatus. Taxa belonging to lineage I was mainly distributed in the Tibetan Plateau of China, North America, and Sweden, while lineage II composed taxa from Northeast China, southern China, and Sweden. The analysis of molecular variance indicated that the genetic difference was mainly due to differences between lineages. Neutrality tests showed that the overall L. variegatus have a stable population since the time of origin. Divergence time analysis suggested that L. variegatus originated from the Triassic period of Mesozoic in 235 MYA (95%HPD: 199–252 MYA), and the divergence between different lineages of L. variegatus began from the next 170 million years. Full article

Geoenergy resources are a new type of clean energy. Carbon neutralization and carbon peaking require significant system reform in the field of energy supply. As a clean, low-carbon, stable and continuous non carbon-based energy, geothermal energy can provide an important guarantee for achieving this goal. Different from the direct way of obtaining energy, ground energy indirectly obtains heat energy from shallow soil and surface water. The vigorous development of geoenergy resources under China’s carbon neutrality goal plays an important role in energy conservation and emission reduction. However, the current carbon trading market is not understood by the public. Therefore, this paper aims to analyze the impact of geoenergy resources on promoting sustainable digital marketing models. Every country around the world is working hard to meet its carbon neutrality goal. This paper analyzed the economic goal of carbon neutrality by analyzing the principle of the carbon trading market. For this reason, this paper designed a carbon trading price prediction algorithm based on the BP neural network (BPNN), which can predict prices in the carbon trading market in order to promote the accurate push of the digital marketing model and finally drive ground energy resources to promote a sustainable digital marketing model. The experimental results of this paper have proved that the price error rate of the BPNN carbon trading price prediction algorithm designed in this paper was within 10%, which was about 20% smaller than the traditional multiple regression analysis algorithm. This proved that the algorithm in this paper has a good performance and can provide accurate information to allow the digital marketing model to achieve sustainable digital marketing. Full article

Accurate detection of cutting diseases in the process of aeroponic rapid propagation is very important for improving the rooting rate and survival rate of cuttings. This paper proposes to use image processing, with a dataset of the growth of mulberry cuttings and a backward propagation (BP) neural network, to identify mildew on the roots of mulberry branches in the process of rapid propagation, before extracting texture and color features. An intelligent control aeroponics system was designed to control the ambient temperature and humidity of the entire rapid propagation incubator according to the mildew rate, thereby improving the rapid propagation time of aeroponics, as well as the rooting and survival rates. In order to distinguish the extracted features, they were classified and identified using a constructed BP neural network model. The results indicated that the performance of the neutral network showed the lowest mean square error in the validation set after three rounds of training; therefore, the model of the third round was chosen as the best model. Furthermore, the training effect of the model revealed that the BP neural network model had good stability and could accurately identify diseases in the root zone of mulberry cuttings. After using MATLAB for neural network training, the regression results revealed correlation coefficients R of 0.98 for the fitting curve of the training dataset, 0.98 for the fitting curve of the test set, and 0.99 for the fitting curve of the validation set, indicating that the prediction results aligned well with the actual results. It can be concluded that research method described in this paper had excellent performance in identifying the health status of mulberry cuttings during the aeroponics rapid propagation process, and it was able to quickly and accurately identify mulberry cuttings affected by mildew disease with an accuracy rate of 80%. This research provides a technical reference for aeroponics rapid propagation factories and intelligent nurseries. Full article

Broussonetia papyrifera has a high lignocellulose content leading to poor palatability and low digestion rate of ruminants. Thus, dynamic profiles of fermentation lignocellulose characteristics, microbial community structure, potential function, and interspecific relationships of B. papyrifera mixing with wheat bran in different ratios: 100:0 (BP100), 90:10 (BP90), 80:20 (BP80), and 65:35 (BP65) were investigated on ensiling days 5, 15, 30, and 50. The results showed that adding bran increased the degradation rate of hemicellulose, neutral detergent fiber, and the activities of filter paper cellulase, endoglucanase, acid protease, and neutral protease, especially in the ratio of 65:35. Lactobacillus, Pediococcus, and Weissella genus bacteria were the dominant genera in silage fermentation, and Pediococcus and Weissella genus bacteria regulated the process of silage fermentation. Compared with monospecific B. papyrifera silage, adding bran significantly increased the abundance of Weissella sp., and improved bacterial fermentation potential in BP65 (p < 0.05). Distance-based redundancy analysis showed that lactic acid bacteria (LAB) were significantly positive correlated with most lignocellulose content and degrading enzymes activities, while Monascus sp. and Syncephalastrum sp. were opposite (p < 0.05). Co-occurrence network analysis indicated that there were significant differences in microbial networks among different mixing ratios of B. papyrifera silage prepared with bran. There was a more complex, highly diverse and less competitive co-occurrence network in BP65, which was helpful to silage fermentation. In conclusion, B. papyrifera ensiled with bran improved the microbial community structure and the interspecific relationship and reduced the content of lignocellulose. Full article

Forest biomass estimation is an important parameter for calculating forest carbon storage, which is of great significance for formulating carbon-neutral strategies and forest resource management measures. We aimed at solving the problems of low estimation accuracy of forest biomass with complex canopy structure and high canopy density, and large differences in the estimation results of the same estimation model under complex forest conditions. The Huangfu Mountain Forest Farm in Chuzhou City was used as the research area. As predictors, we used Gaofen-1(GF-1) and Gaofen(GF-6) satellite high-resolution imaging satellite data, combined with digital elevation model (DEM) and forest resource data. Multiple stepwise regression, BP neural network and random forest estimation models were used to construct a natural subtropical secondary forest biomass estimation model with complex canopy structure and high canopy closure. We extracted image information as modeling factors, established multiple stepwise regression models of different tree types with a single data source and a comprehensive data source and determined the optimal modeling factors. On this basis, the BP neural network and random forest biomass estimation model were established for Pinus massoniana, Pinus elliottii, Quercus acutissima and mixed forests, with the coefficient of determination n (R²) and root mean square error (RMSE) as the judgment indices. The results show that the random forest model had the best biomass estimation effect among different forest types. The R² of Quercus acutissima was the highest, reaching 0.926, but the RMSE was 11.658 t/hm². The R² values of Pinus massoniana and mixed forest were 0.912 and 0.904, respectively. The RMSE reached 10.521 t/hm² and 6.765 t/hm², respectively; the worst result was the estimation result of Pinus elliottii, with an R² of 0.879 and an RMSE of 14.721 t/hm². The estimation result of the BP neural network was second only to that of the random forest model in the four forest types. From high precision to low precision, the order was Quercus acutissima, Pinus massoniana, mixed forest and Pinus elliottii, with R²s of 0.897, 0.877, 0.825 and 0.753 and RMSEs of 17.899 t/hm², 10.168 t/hm², 18.641 t/hm² and 20.419 t/hm², respectively. In this experiment, the worst biomass estimation performance was seen for multiple stepwise regression, which ranked the species in the order of Quercus acutissima, Pinus massoniana, mixed forest and Pinus elliottii, with R²s of 0.658, 0.622, 0.528 and 0.379 and RMSEs of 29.807 t/hm², 16.291 t/hm², 28.011 t/hm² and 23.101 t/hm², respectively. In conclusion, GF-1 and GF-6 combined with data and a random forest algorithm can obtain the most accurate results in estimating the forest biomass of complex tree species. The random forest estimation model had a good performance in biomass estimation of primary secondary forest. High-resolution satellite data have great application potential in the field of forest parameter inversion. Full article