Search Results (8)

Previous studies have shown that AtMP1 and AtMP2, antimicrobial peptides found in the climbing perch (Anabas testudineus), possess antibacterial and anticancer properties. This study aimed to determine whether modified forms of the AtMP2 peptide could enhance its antimicrobial activity. In the research study, the epidermal mucus of Anabas testudineus was extracted and tested to contain antibacterial and anticancer properties. Two peptides were initially extracted from the freshwater fish Anabas testudineus; however, the focus was placed on AtMP2 to evaluate its potential for enhanced antibacterial activity. Systematic-directed evolution was used to generate AtMP2 varieties. The AtMP2 varieties were characterized using APD3, CAMP, and AMPFun. Based on the characterization, two peptides, AtMP2-1 and AtMP2-2, were selected for synthesis. AtMP2-1 and AtMP2-2 demonstrated higher antimicrobial activity against Gram-positive than Gram-negative bacteria compared to the AtMP2 peptide, based on Minimum Inhibitory Concentration (MIC) determination and Kirby–Bauer Disk Diffusion analysis. For the SRB cytotoxicity analysis using the HS-27 and Vero cell lines, both AtMP2-1 and AtMP2-2 are safe for human use at 20 μg/mL. ZDOCK and HPEPDOCK servers were used to evaluate the binding affinity between AtMP2-1 and AtMP2-2 peptides and proteins involved in the bacterial cell death cycle. The results showed varying docking scores, with more negative values indicating stronger binding interactions, particularly with proteins such as SecA, RpoB, GyrA, ClpP, and MetG. The study concluded that modified peptides derived from Anabas testudineus show enhanced antibacterial properties and minimal cytotoxicity, making them potential alternatives to traditional antibiotics. Future research should investigate the specific biochemical pathways affected by these AMPs to understand their mechanisms of action better and explore their potential as therapeutic agents, particularly in the treatment of bacterial infections, wound healing, and cancer therapy. Full article

The climbing perch, Anabas testudineus, is one of the most widely distributed freshwater amphibious fishes in South and Southeast Asia, inhabiting both natural and artificial water bodies polluted by plastic waste. Current mesocosm experimental study aimed to investigate behavioral responses of wild fish to floating expanded polystyrene (EPS) pellets, with a focus on the biofilm developing on their surface. For biofilm formation, the pellets (diameter 3–4 mm) were exposed for two, six, and fourteen days in an irrigation canal inhabited by climbing perch. Development of an intensive biofilm was observed on days 6 and 14 of exposure, characterized by a high diversity of organisms, including protozoa, cyanobacteria, algae, amoebae, and fungi. Fish feeding behavior was observed in the presence of feed pellets, clean EPS pellets, and three variants of EPS pellets with biofilm developed on their surfaces in the freshwater environment. The fish rapidly grasped and ingested feed pellets compared to all variants of plastic pellets. Climbing perch grasped all types of EPS pellets but always rejected them after oral cavity testing. The time to the first grasp was significantly longer for both clean EPS and EPS exposed for two days compared to feed pellets. Biofilm appeared to function as a taste deterrent for the fish: the duration of oral cavity testing was negatively correlated with the EPS pellet exposure timings in natural conditions. We suggest that floating plastic stimulates foraging behavior in the fish, and the duration of this behavior was significantly longer than that observed with feed pellets. The similarity of positive buoyant EPS pellets to natural food objects may stimulate the fish movements towards the water surface, which likely results in greater energy expenditure and increased risk of predation, without any apparent benefit to the individual. Full article

The climbing perch, Anabas testudineus, is one of the most widely distributed freshwater amphibious fishes in South and Southeast Asia, exhibiting terrestrial movements. Our experimental study aimed to investigate endocrinological and biochemical changes in the blood of climbing perch associated with their terrestrial movements. To achieve this, the fish were divided into two groups: one group was exposed to aquatic conditions for twenty minutes, while the other group was subjected to terrestrial conditions for the same duration through rapid water level decrease. In terrestrial conditions, the fish predominantly exhibit movements on land, whereas in aquatic environments, they primarily remain immobile or swim. Elevated levels of stress-induced cortisol and glucose after short-term exposure indicate a high-stress response involving both neuroendocrine and metabolic mechanisms. Changes in the activity of aspartate aminotransferase and increased concentrations of triglycerides in the blood serum suggest energy mobilization through aerobic metabolic pathways. Extreme environmental changes did not affect thyroid axis function, including deiodination, thereby maintaining essential physiological activities under new conditions. Additionally, the anaerobic metabolic pathway appears to be minimally utilized at the onset of terrestrial movement, as no significant changes in lactate dehydrogenase concentrations were observed. Overall, the terrestrial movements of the climbing perch are likely predominantly forced and associated with high stress. Full article

The acute and chronic toxicity of lead to Anabas testudineus was determined in this study using static replacement bioassay testing. During the chronic toxicity studies, an experiment on the bioremediation of lead toxicity using Ocimum sanctum leaf powder was conducted. The 96 h LC₅₀ values of lead for Anabas testudineus was 1.08 mg/L. Different biomarkers, such as the hepatosomatic index, gonadosomatic index, and fecundity, were significantly lower in fish subjected to 10% and 20% of the 96 h LC₅₀ values of lead, compared to controls. The 45-day chronic exposure of fish to lead concentrations of 0.2 mg/L and above significantly lowered the number of total RBC, hemoglobin content, HCT (%), plasma protein, and cholesterol while decreasing the level of total WBC, plasma glucose, creatinine, serum AST and serum ALT. The leaf powder of Ocimum sanctum plays a significant role in ameliorating lead toxicity. Full article

Fish chromosomes are considered homogeneous in their AT/GC nucleotide composition, and banding patterns enabling identification of homologs are largely missing. While cytogenomic approaches try to compensate for this issue by virtual karyotyping, they rely on the quality of genome assemblies available. Recently, soft-masked genome assemblies combining costly and arduous long- and short-read sequencing and new generation assemblers became available for two teleost fish species, climbing perch (Anabas testudineus) and channel bull blenny (Cottoperca gobio). Soft-masking turns repetitive sequences in a genome assembly into lower case letters, leaving unique sequences in upper case. This enables investigators to assess the proportion of guanine and cytosine nucleotides (GC%) of transposable elements as an indicator of AT/GC homogenisation in fish. We have developed a new version of our Python tool Evan, which utilises chromosome-level genome assemblies and combines the profiles of GC% and the proportion of repeats (rep%) along chromosomes. Our profiles of both of those fishes showed clear and abrupt but small-scale fluctuations in GC% along otherwise compositionally homogenised sequences. Our study also highlights the key role of the sliding window size in determining the resolution of GC% profiling. While the quality of the genome assemblies appeared to be sufficient for GC%/rep% profiling, more effective repeat masking is necessary to better distinguish to what extent repeats compositionally homogenize fish genomes. Full article

The climbing perch Anabas testudineus is widespread in the inland waters of Vietnam and according to its ecology could have contact with floating plastic waste. Fragments of expanded polystyrene (EPS) are detected in the fresh waters of Vietnam in Khanh Hoa, Lam Dong, and Phu Yen provinces. Our study focused on estimating the probability of ingestion of EPS pellets (size 2.5–3.5 mm) by adult climbing perch. In the experiments, 3 types of treatment pellets were offered to fish: 24 feed pellets (Fps), 24 expanded polystyrene pellets (Pps), and 12 feed and 12 expanded polystyrene pellets (FPps). Fish grasping time of the first pellet was independent in all treatment types. The grasping time of the 12th pellet was insignificant in Fps (63 s) and Pps (75 s). Climbing perch grasped and ingested the 24th Fp significantly (p = 0.02) earlier (143 s), than they grasped the 24th Pp (817 s). Fish with FPp treatment grasped feed along with EPS pellets, but grasping the 12th Fp was significantly (p = 0.02) earlier (49 s) than the 12th Pp (193 s). By the end of the tests, the fish had ingested all feed pellets. We discovered that climbing perch grasped Pps and kept them in the oral cavity, but rejected them in 100% of the cases. This result provided evidence that climbing perch have an effective defense mechanism for avoiding ingestion of expanded polystyrene pellets with a size of 2.5–3.5 mm. Full article

The present study was conducted to understand the changes in gonads and hematological parameters in climbing perch (Anabas testudineus) exposed to synthetic estrogen and progestogen [mixture of ethinylestradiol (EE2) and desogestrel (DES)]. Climbing perch were exposed to four different concentrations of EE2/DES mixtures, viz. 0 ng of EE2 and DES/L (T0), 3 ng EE2 and 15 ng DES/L (T3), 30 ng EE2 and 150 ng DES/L (T30), and 300 ng EE2 and 1500 ng DES/L (T300) for 60 days. On days 45 and 60, samples were taken to assess changes in somatic indexes, gonad histology, and hematological parameters. The gonadosomatic index (GSI) increased in both females and males with increasing concentrations of estrogen mixtures except for T30 females, which was the lowest among all\four treatments. The hepatosomatic index (HSI) was observed to be increased in males as estrogen content increased. However, compared to fish at T0, HSI in female individuals did not vary in T30 fish, where the value was the highest among all the treatments. On day 45, histological observations showed no feminization or intersexuality but several germ-cell deformities in the ovary (adhesion, degenerated oocyte wall, degenerated granulose layer, increased interfollicular space, atretic follicle, and cytoplasmic clumping) and testes (increased interstitial area, focal loss of spermatocyte, dilation of the lumen, breakage of tubular epithelium, and elongated seminiferous tubule) were observed in fish exposed to EE2 and DES. Fish reared at T30 had lower RBC count, hemoglobin (Hb), glucose, and hematocrit levels. On day 60, fish reared at T30 had the highest Hb content compared to fish raised in other treatment conditions. WBC was progressively higher with increasing EE2/DES concentrations. Significant erythrocyte cytoplasmic abnormalities and erythrocyte nuclear abnormalities were observed in fish exposed to higher EE2/DES concentrations. The present study provides insights into the adverse impacts of synthetic estrogens sourced from human contraceptive pills on fish physiology. Full article

Substantial quantities of pesticides are routinely applied to enhance agricultural crop production. Pesticides used in this way continuously accumulate in the environment and in foods. Harvested crops contain pesticide residues at various concentrations, with potential harmful impacts on human health. Hence, it is of value to identify techniques for the effective decontamination of tainted foods. However, cleaning with water or household agents, e.g., acetic acid and sodium bicarbonate, are recognized treatments for the efficient degradation of pesticides from vegetables and fruits. There is an apparent void of information about the decontamination treatments for raw fishes using household agents that are affordable for all classes of consumers. Hence, the present study was performed to determine the most efficient household technique for reducing pesticide residue levels from precooked raw fish to ensure the utmost food safety. Fish muscles of four species of fishes, viz., Clarias gariepinus, Channa striatus, Anabas testudineus and Trichogaster trichopterus, were treated with six treatments: washing with running tap water (T₁), dipping in normal water (T₂), dipping in 2% salt solution (T₃), dipping in 2% vinegar (T₄), dipping in 0.1% sodium bicarbonate solution (T₅) as well as dipping in 0.1% sodium bicarbonate solution + 2% vinegar + 2% salt solution + lemon juice (T₆), as fish muscle is the major consumable portion of fish. The current study demonstrated that the removal percentage of lindane, heptachlor, aldrin, endosulfan, dieldrin, endrin, DDT, methoxychlor and cypermethrin residues against the treated household treatments, in downward order, were soaking in 0.1% sodium bicarbonate solution + 2% vinegar + 2% salt solution + lemon juice solution (T₆) > soaking in 2% vinegar (T₄) solution > soaking in 0.1% sodium bicarbonate (T₅) solution > soaking in 2% salt (T₃) solution > washing with running tap water (T₁) > soaking in stable normal water (T₂). The treatment of raw fish muscle samples by soaking them in 0.1% sodium bicarbonate solution + 2% vinegar + 2% salt solution + lemon juice was found to be the most efficient household treatment, performing significant reductions (%) in pesticide concentration: 72–80% (p < 0.05) in Channa striata, 71–79% (p < 0.05) in Clarias gariepinus, 74–80% (p < 0.05) in Anabas testudineus as well as 78–81% (p < 0.05) in Trichogaster trichopterus before cooking. Full article