Search Results (3,478)

Wine, as a high-value product, is vulnerable to counterfeiting. To tackle increasingly sophisticated fraud, innovative analytical approaches are required. However, they must undergo rigorous validation. Proton nuclear magnetic resonance spectroscopy (¹H-NMR) is intrinsically quantitative, reproducible, and fast, making it a promising tool for official control. This study presents the development and validation of a standardised and fully automated workflow for the quantification of 20 oenologically relevant compounds, including organic acids, sugars, alcohols, esters, phenolics, and an alkaloid. The method combines optimised sample preparation, external quantification standards, spectrometer calibration, and a dedicated R package (RnmrQuant1D) for fully automated spectral processing, enabling high-throughput and operator-independent analysis. Validation was performed under intermediate precision according to OIV metrological standards, evaluating accuracy, precision, robustness, limits of quantification, and measurement uncertainty. The results demonstrated excellent linearity, trueness, and reproducibility, matching the targeted analytical performance. Measurement uncertainties were estimated both by conventional linear modelling and by a dynamic approach better suited to detection limits. The workflow is easy to implement, requires minimal sample consumption, and substantially reduces operator bias. Beyond validating a robust method, this study provides a framework for harmonised, transferable ¹H-NMR workflows that could support large-scale databases, integration with chemometric models, and ultimately, ¹H-NMR’s recognition as a relevant method for wine authentication and quality control. This work fills a crucial gap in wine analysis by uniting practical application and rigorous methods, enabling broader adoption in control laboratories worldwide. Full article

Ureaplasma species are associated with various reproductive health issues, while human papillomavirus (HPV) is associated with cervical, vaginal and vulvar cancers. Data on the association between Ureaplasma species and HPV are limited in South Africa. This study investigated the prevalence of Ureaplasma urealyticum (U. urealyticum), Ureaplasma parvum (U. parvum), and HPV coinfection and their associated factors, among adolescent girls and young women (AGYW) in the Eastern Cape Province, South Africa. A total of 214 participants were retrospectively recruited, and secondary data on HPV, U. urealyticum, U. parvum, demographics, and sexual behavior were used. HPV was detected using the Roche Linear Array HPV Genotyping Test, while U. urealyticum and U. parvum were detected using Allplex™ sexually transmitted infection (STI) essential Assay. Statistical analyses were performed using GraphPad Prism Version 8.0.1.244. The prevalence of U. urealyticum was 43.9% (94/214) and increased significantly with age (p = 0.036, R² = 0.8497); while U. parvum prevalence was 68.7% (147/214) and was not influenced by age. Having four to six lifetime sexual partners (PR: 1.77, 95% CI: 1.04–3.00, p = 0.043) was associated with increased risk of U. urealyticum. A proportion of 36.3% (77/212) had HPV-U. urealyticum coinfection and its risk was increased among those with 3–6 lifetime sexual partners (PR: 1.59, 95% CI: 1.10–2.53, p = 0.017), 2–4 new partners past three months (PR: 2.14, 95% CI: 1.19–2.42, p = 0.021); vaginal sexual intercourse frequency past 1-month (2–3 vaginal intercourse: PR: 1.54, 95% CI: 1.06–2.53, p = 0.037; 4–10 vaginal intercourse: PR: 1.91, 95% CI: 1.83–1.91, p = 0.005) and alcohol consumption (PR: 1.85, 95% CI: 1.20–3.28, p = 0.004). U. urealyticum positives had a significantly higher risk of HPV types targeted by Cervarix^® HPV vaccine than negatives (PR: 2.56, 95% CI: 1.23–5.37, p = 0.013), Gardasil^®4 (PR: 2.16, 95% CI: 1.25–3.75, p = 0.006) and Gardasil^®9 (PR: 1.70, 95% CI: 1.25–2.32, p = 0.001). AGYW of Eastern Cape Province, South Africa had high prevalence of U. urealyticum-HPV and U. parvum-HPV coinfections. Ureaplasma species coinfection was associated with HPV prevalence and distribution of genotypes. The U. urealyticum prevalence and its coinfection with HPV were associated with sexual behavior. Data from this study could contribute to the design of sexual health and STI interventions and could serve as a baseline for future epidemiological studies, which include ongoing surveillance of HPV genotype prevalence to evaluate the impact and effectiveness of HPV vaccination programs in the population. Full article

The growing interest in no- and low-alcohol (NoLo) wines reflects evolving consumer preferences toward moderation, health, and mindful drinking. This study investigates consumer perception and acceptance of NoLo wines within a traditional wine context (Trentino-Alto Adige, Italy), based on a survey of 344 people. Respondents were primarily between 18 and 34 years old. Descriptive results indicated low familiarity and purchase frequency but positive attitudes, especially among women and health-oriented consumers. Nonparametric tests revealed that gender significantly affected familiarity, social acceptance, and willingness to recommend NoLo wines, with women respondents showing higher engagement and acceptance. Age showed a weaker but still significant effect on familiarity, while consumers who regularly consumed NoLo beverages exhibited greater social acceptance and willingness to recommend. In addition, logit and probit models suggested that preference for mid-to-low alcohol levels and prior experience with alcohol-free drinks positively influenced purchase frequency. In contrast, traditional wine consumption habits and expenditure had no significant effects. These findings suggest that while NoLo wine adoption in a premium wine region such as Trentino-Alto Adige is in an emerging phase, it is underpinned by evolving young consumer motivations toward moderation, well-being, and social inclusivity, offering clear opportunities for targeted market development and product innovation. Full article

Background/Objectives: Craft non-alcoholic and low-alcohol beer (NABLAB) is attracting increasing attention as potential functional beverages due to their content of bioactive compounds such as polyphenols, vitamins, and minerals, and their suitability for health-oriented lifestyles. This study investigated Italian consumers’ perceptions of craft NABLAB and explored possible generational differences in their acceptance. Methods: A descriptive cross-sectional online survey was conducted between March 2024 and March 2025 among adults living in Italy. The questionnaire, composed entirely of closed-ended questions, investigated familiarity with craft NABLAB, attitudes toward their potential health-related properties, and willingness to recommend them. Results: A total of 527 valid responses were analyzed descriptively and grouped by generation (Generation Z, Millennials, Generation X, and Baby Boomers). Results showed that 68.3% of participants would recommend craft NABLAB to others interested in their functional properties, while 55.0% reported higher motivation to purchase when informed about their potential health benefits. Familiarity with these products remained limited (34.7% had tried them, and only 22.2% considered them easy to find). Baby Boomers and Millennials were more receptive, possibly due to greater health awareness and openness to innovation, whereas Generation Z displayed curiosity despite lower consumption experience. Conclusions: Overall, Italian consumers show a growing interest in craft NABLAB, especially when linked to wellness and active lifestyle benefits. Enhancing product availability and communication focused on health and functionality could promote more moderate and conscious drinking habits, contributing to a gradual cultural shift toward reduced alcohol consumption. Full article

The current investigation examines pathways linking individual risk factors, namely maladaptive emotion regulation, behavior problems, and drinking motives, with adolescents’ alcohol consumption. In a cross-sectional design, 243 adolescents attending secondary school in Germany completed questionnaires. The Cognitive Emotion Regulation Questionnaire was used to assess maladaptive emotion regulation, the Strengths and Difficulties Questionnaire for assessing internalizing and externalizing behaviors, and the Drinking Motives Questionnaire Revised for assessing the four drinking motives, namely social, enhancement, coping, and conformity motives. Adolescents also reported their daily and problematic alcohol consumption. The path analysis revealed that maladaptive emotion regulation was positively associated with both internalizing and externalizing problems, and all four drinking motives. Externalizing problems were positively associated with adolescents’ enhancement and coping motives, and their alcohol consumption. Internalizing problems were only negatively associated with enhancement motives. Only coping motives were positively associated with alcohol consumption. Moreover, maladaptive emotion regulation had an indirect effect on alcohol consumption, via externalizing problems and coping motives. The findings emphasize the interactions between the risk factors in contributing to adolescent alcohol consumption, underscoring the importance of targeting emotion regulation and coping motives in substance use prevention efforts prior and during adolescence. Full article

This study assessed problematic mobile phone use in the Spanish population between 2014 and 2025 using the Mobile Phone Problematic Use Scale (MPPUS) and the Mobile Phone Addiction Craving Scale (MPACS). Two online surveys were conducted in 2018 (n = 1612) and 2024 (n = 2001) across Spain’s 17 autonomous communities, with analyses by gender, age, occupation, education level, and population size. Data were compared with a 2014 baseline study (n = 1126). The prevalence of problematic mobile phone use declined slightly from 5.1% in 2014 to 4.8% in 2018 and 3.2% in 2024. Users reporting difficulties controlling their phone use (problematic and at-risk users) also decreased from 20.5% in 2014 to 18.8% in 2024. However, the severity of problematic use increased over the decade, as reflected by rising MPACS scores among problematic users. Major factors associated with problematic use included hours of daily use, age (especially under 35 years), anxiety symptoms, and videogaming, while gender and alcohol consumption showed minor influence. Despite the slight reduction in prevalence, the growing intensity of problematic use highlights its persistence as a public health concern among young adults, underscoring the need for preventive and therapeutic interventions. Full article

Background: Acute coronary syndrome (ACS) encompasses ST-elevation myocardial infarction, non-ST-elevation myocardial infarction, and unstable angina. While optimal medical therapy (OMT) is central to secondary prevention, lifestyle interventions—particularly dietary modification—remain underutilised despite their potential impact on long-term outcomes. Objective: To review the current evidence regarding dietary interventions post-ACS, their implementation, adherence, and effects on cardiovascular risk factors and clinical outcomes. Methods: A narrative literature review was performed using PubMed, including studies published in English from 2000 onwards. Keywords included “acute coronary syndrome,” “diet,” “cardiovascular disease,” “outcomes,” “adherence,” “wine,” and “intermittent fasting,” combined with Boolean operators AND/OR. Animal studies were excluded. The latest search was conducted in October 2025. Results: Mediterranean-style diets, when combined with OMT and lifestyle interventions (exercise, smoking cessation, alcohol moderation), consistently improve cardiovascular risk factors and reduce recurrent ischemic events and mortality. Clinical trials and cohort studies demonstrate long-term benefits, including reductions in all-cause mortality and major adverse cardiovascular events, particularly in patients adhering to structured dietary programmes within cardiac rehabilitation. Evidence for other dietary modifications, including low-fat diets, increased fibre, antioxidant supplementation, and intermittent fasting, was more limited, often derived from small or short-term studies focusing on surrogate endpoints. Real-world adherence to dietary guidelines remains suboptimal, especially in high-risk and obese populations. Preliminary studies suggest intermittent fasting and moderate red wine consumption may confer additional cardiovascular benefits, though larger, long-term trials are needed. Conclusions: Dietary modification is a key, yet underutilised component of secondary prevention post-ACS. A Mediterranean-style, whole-food diet integrated with OMT and supported by structured cardiac rehabilitation programmes offers the most evidence-based strategy to improve risk factor control and long-term outcomes. Future research should focus on pragmatic, long-term trials assessing hard cardiovascular endpoints and implementation strategies to enhance adherence across diverse populations. Full article

Poly(vinyl chloride) (PVC) is one of the most widely used polymers due to its strength, low cost, and light weight. Industrial production is mainly conducted by suspension polymerization, which facilitates the control of the emissions of vinyl chloride monomer (VCM), a known carcinogen. However, the process consumes large amounts of water and energy and generates residual compounds such as polyvinyl alcohol (PVA) and polymerization initiators, which must be properly managed to mitigate environmental impacts. To improve sustainability, this study applied mass- and energy-integration strategies together with a zero-liquid-discharge (ZLD) water-regeneration system that uses sequential aerobic and anaerobic reactors to recirculate process water with reduced PVA. Although these measures reduce resource consumption, they can displace or intensify other impacts; therefore, a comprehensive evaluation of the system is necessary. Accordingly, the objective of this study is to quantify and compare the potential environmental impacts (PEIs) of the improved PVC production process through a scenario-based assessment using a waste reduction algorithm (WAR). This is applied to four operating scenarios in order to identify the stages and flows that contribute most to the environmental burden. According to our literature review, there is limited published evidence that simultaneously combines mass/energy integration and a ZLD system in PVC processes; thus, this work provides an integrated assessment useful for industrial design. The environmental performance of the improved process was evaluated using WAR GUI software (v 1.0.17, which quantifies PEIs in categories such as toxicity, climate change, and acidification. Four scenarios were compared: Case 1 (excluding both product and energy), Case 2 (product only), Case 3 (energy only), and Case 4 (product and energy). The total PEI increased from 2.46 PEI/day in Case 1 to 6230 PEI/day in Case 4, with the largest contributions from acidification (5140 PEI/day) and global warming (496 PEI/day), mainly due to natural gas consumption (5184 GJ/day). In contrast, Cases 1 and 2 showed negative PEI values (−3160 and −2660 PEI/day), indicating that converting the toxic VCM (LD₅₀: 500 mg/kg; ATP: 26 mg/L) into PVC (LD₅₀: 2000 mg/kg; ATP: 100 mg/L) can reduce the environmental burden in certain respects. In addition, the ZLD system contributed to maintaining low aquatic toxicity in Case 4 (90.70 PEI/day). Full article

Plants have been used in medicine for centuries to treat various diseases, with alcohol and ethanol being known as universal solvents for the extraction of medicinal plant substances. This article sheds light on Artemisia absinthium (wormwood) and absinthe usage in the history of medicine. The invention of absinthe in Switzerland in 1797 made it an integral part of everyday life and the harmful effects of the massive consumption of this product were labelled absinthism. The medicinal properties of wormwood and absinthe are explored from the earliest records of the use of wormwood from the Ebers Papyrus (copies of which date back to 1550 BC) to the military consumption of absinthe during the French invasion of Algiers in 1830. As widely accepted, A. absinthium has both anthelmintic and antiprotozoal properties. In addition, modern medicine has demonstrated antibacterial, antifungal and antibiofilm properties of the plant extracts. In order to fully utilise the therapeutic potential of A. absinthium, advances in pharmaceutical technology are essential. One promising solution could lie in nanotechnological delivery systems. In our opinion absinthe is another impressive example of how tonics containing various herbal substances were used in the history of medicine to manage infections before their efficacy was later proven in vitro and in vivo. Full article

Alcohol misuse is twice as prevalent among people living with HIV (PWH), and this increases the risk of pulmonary complications even in those receiving antiretroviral therapy. Our prior work showed that the alcohol metabolite, acetaldehyde, activates nuclear factor kappa B p65 (p65), leading to HIV replication and interleukin (IL)-1β activation in alveolar macrophages (AMs). Since the aforementioned processes are energy-demanding, which conversely impair mitochondrial functions, we hypothesized that acetaldehyde-induced p65 drives AMs to a mitochondrial hyperactive state to promote HIV replication and IL-1β release and induces oxidative stress and mitochondrial dysfunction. Since we found pioglitazone (PIO) to be a negative regulator of p65, we postulate that PIO suppresses HIV replication and IL-1β activation in AMs by restricting p65-induced mitochondrial hyperactivation. Murine AMs were exposed to acetaldehyde via the acetaldehyde generating system (AGS) and infected in vitro with EcoHIV, a chimeric ecotropic HIV construct. AGS + EcoHIV activated p65, resulting in enhanced ATP-linked mitochondrial respiration, proton leak, non-mitochondrial respiration and the generation of reactive oxygen species (ROS) in AMs. Inhibition of mitochondrial ATP synthesis with low-dose oligomycin attenuated AGS-induced HIV replication and AGS + EcoHIV-induced IL-1β release from AMs. PIO treatment, which attenuated AGS-induced p65 activation, suppressed proton leak, non-mitochondrial oxygen consumption, ROS, and IL-1β and p24 release. While p65-induced mitochondrial hyperactivation represents AMs’ adaptive response to the energy demands imposed by HIV replication and proinflammatory activation when exposed to acetaldehyde, PIO treatment may offer a novel therapeutic strategy to restore adequate mitochondrial bioenergetics in the AMs of PWH who misuse alcohol. Full article

Objective: Cardiovascular diseases have a high prevalence among the elderly, together with frailty syndrome, and both conditions negatively affect quality of life and limit patient autonomy. This study aimed to explore potential relationships between cardiovascular and metabolic parameters, renal function, and frailty domains to identify potential intervention targets. Methods: A cross-sectional study was conducted between January 2024 and April 2025 at the National Institute of Gerontology and Geriatrics “Ana Aslan”, including 359 patients aged over 40 years. Demographic, anthropometric, and clinical data were collected through interviews, medical records, and standardized assessments of frailty components (weakness, exhaustion, slow gait, balance impairment, reduced activity, cognitive decline, and weight loss), as well as cardiovascular diseases and comorbidities. Results: Most participants were aged 65–79 years. ROC curve identified triglycerides as a good indicator of both alcohol consumption (AUC = 0.631, p = 0.042) and smoking status (AUC = 0.676, p = 0.004), while HDL cholesterol showed an inverse association with smoking status (AUC = 0.356, p = 0.019). Reduced renal function was significantly associated with smoking status, balance, gait impairment, and reduced functional mobility. The Up and Go Test indicated a good discriminatory ability for renal function decline (AUC = 0.656, p < 0.001). Muscle strength, MMSE, and Tinetti scores showed inverse associations with renal function. Conclusions: Renal impairment appears to be a reliable indicator across multiple frailty domains, acting as an accelerator of frailty progression. Triglycerides reflect lifestyle-related factors, while the Up and Go Test may serve as a practical screening tool for renal dysfunction in frail older adults. These findings suggest the need to adapt traditional cardiovascular risk management to the frail geriatric population. Full article

Metabolic dysfunction–associated fatty liver disease (MASLD) is associated with severe forms of liver injury, including fibrosis and cirrhosis. The main risk factors for MASLD—obesity, type 2 diabetes mellitus (T2DM), dyslipidemia, and insulin resistance (IR)—contribute to metabolic disturbances that initiate hepatic steatosis. Metabolic and alcohol-related liver disease (MetALD) describes patients with MASLD who also present alcohol-associated hepatic injury. Chronic oxidative and inflammatory stress promotes the progression of steatosis in both conditions. T2DM and chronic alcohol consumption are independent lifestyle-related risk factors for cirrhosis within the spectrum of metabolic dysfunction–related liver disease (MASLD and MetALD). The coexistence of both conditions may exacerbate hepatic pathological alterations. IR, which is frequently observed in patients with cirrhosis, can lead to the development of a condition known as hepatogenic diabetes (HD). HD is characterized by hyperinsulinemia, IR, and β-cell dysfunction occurring during the onset of cirrhosis and is associated with hepatic inflammation even in the absence of traditional metabolic risk factors such as obesity or a prior history of T2DM. In this context, alcohol intake enhances lipolysis in peripheral tissues, promotes hepatic steatosis, and aggravates metabolic dysfunction, ultimately contributing to excessive mitochondrial production of reactive oxygen species (ROS). Therefore, the present review examines the role of oxidative stress—both alcohol-related and non-alcohol–related—in the pathogenesis of HD, with particular emphasis on ethanol metabolism, oxidative stress, and their interactions in conditions such as T2DM and MetALD. Full article

Background: Lifestyle factors often interact in complex ways when influencing chronic disease risk. We aimed to examine the prospective associations between empirically derived real-life lifestyle patterns (LPs) and the incidence of major chronic diseases, and to explore the linearity of the relationships between lifestyle summation scores and disease risk. Methods: We included adults free of cardiovascular diseases (CVDs), cancer, chronic respiratory diseases (CRDs), or type 2 diabetes (T2D) at baseline (2006–2013) from the Dutch Lifelines cohort. LPs and lifestyle summation scores were derived from baseline self-reported data on diet, physical activity, substance use, sleep, stress, and social connectedness, each categorised as healthy, moderately healthy, or unhealthy. Fine–Gray sub-hazard regression models assessed associations between LPs and disease incidence, with natural spline functions used to evaluate linearity in summation scores. Results: Among 114,919 T2D-free, 131,248 cancer-free, 91,777 CRD-free, and 77,645 CVD-free participants, we observed 3114 T2D, 4685 cancer, 4133 CRDs, and 2850 CVD incident cases (median follow-up time: 8 years). Compared to the “Unhealthy” pattern, both the “Healthy-in-a-balanced-way” and “Healthy-but-physically-inactive” patterns were broadly significantly protective. The “Unhealthy-but-no-substance-use” pattern was associated with increased T2D risk (Sub-Hazard Ratio (SHR) = 1.27, 95% Confidence Interval (CI): 1.11–1.47) but reduced cancer risk (SHR = 0.85, 95%CI: 0.74–0.97). The “Unhealthy-but-light-drinking-and-never-smoked” pattern was protective for T2D (SHR = 0.89, 95%CI: 0.79–0.99). Linear associations were observed between lifestyle summation scores and disease risk, except for “healthy lifestyle” scores with T2D and “unhealthy lifestyle” scores with CRDs (non-linear p-value < 0.05). Conclusions: There are potential protective effects of healthy lifestyles on T2D, cancer, CRDs, and CVDs. However, the “Unhealthy but no substance use” demonstrated increased risk on T2D, protective effect on cancer and no significant effect on CRDs or CVDs. The relationship between combined lifestyle factors and NCD risk is complex and partly non-linear, showing diminishing benefits beyond certain thresholds, especially T2D and CRDs. Full article

Background/Objectives: In recent years, electric scooters have gained widespread popularity as an easy and affordable mode of transport in urban areas worldwide. Simultaneously, trauma centers have observed an increasing number of associated injuries to users. While injury patterns associated with other vehicles are now well-researched, electric-scooter-related injuries are a new topic in the literature. Our study aims to investigate the differences in injury patterns and other critical crash characteristics among riders of bicycles, electric scooters, and scooters. Methods: This one-year retrospective observational study examined patients who sustained injuries while riding bicycles, electric scooters, or scooters between April 2021 and March 2022 at Hungary’s largest trauma center in Budapest. During this one-year period, we identified 1938 patients, 1378 cyclists, 370 electric scooter users, and 190 scooter users. Basic demographic information, recorded injury type and severity, time of day the injury occurred, and alcohol usage were recorded as outcome measures. Results: While 4.6% of cyclists and 5.8% of scooter riders had consumed alcohol, 26.8% of electric scooter riders were under the influence of alcohol at the time of their crash. Of electric-scooter-related injuries, 45.8% occurred at night, compared to only 9.2% and 14.1% of bike and scooter-related injuries, respectively. E-scooter crashes constituted 19.1% of total cases but surged to 52.3% at night. Patients under the influence of alcohol were much more likely to experience mild head injuries (p < 0.0001) and severe head injuries (p < 0.0001), but less likely to suffer mild limb injuries (p < 0.0001) and severe limb injuries (p < 0.0001) compared with sober patients. Cyclists had significantly 3 times fewer cases of severe head trauma than those injured while using electric scooters (p = 0.0166). Conclusions: The study highlights a significant risk of severe craniofacial injuries in e-scooter users after consuming alcohol, exceeding that in sober riders and cyclists. Predominantly occurring at night, these injuries are closely linked with alcohol use. The findings advocate for mandatory helmet laws and stricter regulations on e-scooter use to enhance safety, especially at night. Full article

Background: Postoperative delirium (POD) is one of the most common surgical complications in elderly patients. This study investigated the incidence of and perioperative risk factors for POD following urological surgery. Methods: A total of 162 male patients aged ≥50 years undergoing elective major urological surgery under general anesthesia from May 2024 to March 2025 were included in this prospective observational study. Delirium was assessed using CAM-ICU twice a day for five postoperative days. Groups with and without delirium were compared, and perioperative predictors of delirium were analyzed. Multivariate regression analysis was used to identify independent risk factors for POD. Results: Overall, 16% of patients developed delirium during the follow-up period. Patients with POD were significantly older (mean age, 73.3 ± 5.2 years vs. 66.3 ± 7.2, p < 0.001), had more comorbidities, and lived in rural areas. Atrial fibrillation and COPD were particularly significant. The incidence of POD was higher in patients with mild/moderate alcohol consumption than in those who never drink. Analysis of intraoperative factors revealed a significant difference between groups in the presence of intraoperative hypotension and blood transfusion. Patients with delirium had more severe postoperative pain. Optimal cutoff values of age (≥67.5), number of comorbidities (≥2), preoperative MMSE score (≤25.5), and postoperative NRS score (≥4.85) were determined using ROC curves. The multivariate analysis identified age ≥ 67.5 years, COPD, mild/moderate alcohol consumption, preoperative MMSE score ≤ 25.5, intraoperative hypotension, and postoperative NRS score ≥ 4.85 as independent risk factors in this cohort. Conclusions: Considering that some of the above risk factors can be modified, it is necessary to emphasize that the prevention of POD is possible and should be one of the treatment priorities in older patients. Full article