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Authors = Xiaofei Hu

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24 pages, 3480 KiB  
Article
MFPI-Net: A Multi-Scale Feature Perception and Interaction Network for Semantic Segmentation of Urban Remote Sensing Images
by Xiaofei Song, Mingju Chen, Jie Rao, Yangming Luo, Zhihao Lin, Xingyue Zhang, Senyuan Li and Xiao Hu
Sensors 2025, 25(15), 4660; https://doi.org/10.3390/s25154660 - 27 Jul 2025
Viewed by 387
Abstract
To improve semantic segmentation performance for complex urban remote sensing images with multi-scale object distribution, class similarity, and small object omission, this paper proposes MFPI-Net, an encoder–decoder-based semantic segmentation network. It includes four core modules: a Swin Transformer backbone encoder, a diverse dilation [...] Read more.
To improve semantic segmentation performance for complex urban remote sensing images with multi-scale object distribution, class similarity, and small object omission, this paper proposes MFPI-Net, an encoder–decoder-based semantic segmentation network. It includes four core modules: a Swin Transformer backbone encoder, a diverse dilation rates attention shuffle decoder (DDRASD), a multi-scale convolutional feature enhancement module (MCFEM), and a cross-path residual fusion module (CPRFM). The Swin Transformer efficiently extracts multi-level global semantic features through its hierarchical structure and window attention mechanism. The DDRASD’s diverse dilation rates attention (DDRA) block combines convolutions with diverse dilation rates and channel-coordinate attention to enhance multi-scale contextual awareness, while Shuffle Block improves resolution via pixel rearrangement and avoids checkerboard artifacts. The MCFEM enhances local feature modeling through parallel multi-kernel convolutions, forming a complementary relationship with the Swin Transformer’s global perception capability. The CPRFM employs multi-branch convolutions and a residual multiplication–addition fusion mechanism to enhance interactions among multi-source features, thereby improving the recognition of small objects and similar categories. Experiments on the ISPRS Vaihingen and Potsdam datasets show that MFPI-Net outperforms mainstream methods, achieving 82.57% and 88.49% mIoU, validating its superior segmentation performance in urban remote sensing. Full article
(This article belongs to the Section Sensing and Imaging)
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27 pages, 13439 KiB  
Article
Swin-ReshoUnet: A Seismic Profile Signal Reconstruction Method Integrating Hierarchical Convolution, ORCA Attention, and Residual Channel Attention Mechanism
by Jie Rao, Mingju Chen, Xiaofei Song, Chen Xie, Xueyang Duan, Xiao Hu, Senyuan Li and Xingyue Zhang
Appl. Sci. 2025, 15(15), 8332; https://doi.org/10.3390/app15158332 - 26 Jul 2025
Viewed by 183
Abstract
This study proposes a Swin-ReshoUnet architecture with a three-level enhancement mechanism to address inefficiencies in multi-scale feature extraction and gradient degradation in deep networks for high-precision seismic exploration. The encoder uses a hierarchical convolution module to build a multi-scale feature pyramid, enhancing cross-scale [...] Read more.
This study proposes a Swin-ReshoUnet architecture with a three-level enhancement mechanism to address inefficiencies in multi-scale feature extraction and gradient degradation in deep networks for high-precision seismic exploration. The encoder uses a hierarchical convolution module to build a multi-scale feature pyramid, enhancing cross-scale geological signal representation. The decoder replaces traditional self-attention with ORCA attention to enable global context modeling with lower computational cost. Skip connections integrate a residual channel attention module, mitigating gradient degradation via dual-pooling feature fusion and activation optimization, forming a full-link optimization from low-level feature enhancement to high-level semantic integration. Simulated and real dataset experiments show that at decimation ratios of 0.1–0.5, the method significantly outperforms SwinUnet, TransUnet, etc., in reconstruction performance. Residual signals and F-K spectra verify high-fidelity reconstruction. Despite increased difficulty with higher sparsity, it maintains optimal performance with notable margins, demonstrating strong robustness. The proposed hierarchical feature enhancement and cross-scale attention strategies offer an efficient seismic profile signal reconstruction solution and show generality for migration to complex visual tasks, advancing geophysics-computer vision interdisciplinary innovation. Full article
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8 pages, 2367 KiB  
Article
Microwave-Controlled Spectroscopy Evolution for Different Rydberg States
by Yinglong Diao, Haoliang Hu, Xiaofei Li, Zhibo Li, Feitong Zeng, Yanbin Chen and Shuhang You
Photonics 2025, 12(7), 715; https://doi.org/10.3390/photonics12070715 - 16 Jul 2025
Viewed by 225
Abstract
In this paper, a series of electromagnetically-induced-transparent (EIT) spectra of different Rydberg states, controlled by microwaves, in rubidium (Rb) thermal vapor are presented. The novel evolution regularity for different Rydberg states can be found by experimentally detected transmitted EIT spectra, which can reveal [...] Read more.
In this paper, a series of electromagnetically-induced-transparent (EIT) spectra of different Rydberg states, controlled by microwaves, in rubidium (Rb) thermal vapor are presented. The novel evolution regularity for different Rydberg states can be found by experimentally detected transmitted EIT spectra, which can reveal the primary quantum number of different Rydberg states and how to influence microwave control spectroscopy evolution regularity, and which can pave the way in order to address the challenge of selecting Rydberg states for applications in Rydberg microwave field detection. This is helpful for the development of measuring standards of the microwave field in Rydberg states. Full article
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17 pages, 2198 KiB  
Article
Jujube–Cotton Intercropping Enhances Yield and Economic Benefits via Photosynthetic Regulation in Oasis Agroecosystems of Southern Xinjiang
by Shuting Zhang, Jinbin Wang, Zhengjun Cui, Tiantian Li, Zhenlin Dong, Hang Qiao, Ling Li, Sumei Wan, Xiaofei Li, Wei Zhang, Qiang Hu and Guodong Chen
Agronomy 2025, 15(7), 1676; https://doi.org/10.3390/agronomy15071676 - 10 Jul 2025
Viewed by 423
Abstract
This study aimed to clarify the effects of jujube–cotton intercropping on cotton yield and photosynthetic characteristics, providing a theoretical basis for its application in the oasis irrigation areas of southern Xinjiang and offering practical recommendations to local farmers for increasing economic benefits. The [...] Read more.
This study aimed to clarify the effects of jujube–cotton intercropping on cotton yield and photosynthetic characteristics, providing a theoretical basis for its application in the oasis irrigation areas of southern Xinjiang and offering practical recommendations to local farmers for increasing economic benefits. The effects were investigated from 2020 to 2023 using Zhongmian 619 cotton and juvenile jujube trees. Changes in leaf area index (LAI), transpiration rate (Tr), stomatal conductance (Gs), net photosynthetic rate (Pn), intercellular CO2 concentration (Ci), yield, and economic benefits were evaluated over the years. The results showed that (1) a positive correlation was observed between LAI and the photosynthetic characteristics of cotton. Compared to monoculture cotton, intercropped cotton exhibited lower Pn, Gs, and Tr, and at the peak boll stage, monoculture cotton had significantly higher photosynthetic characteristics, indicating that intercropping affected cotton photosynthesis. (2) From 2020 to 2023, the land equivalent ratio (LER) of jujube–cotton intercropping remained above 1, with overall yield and economic benefit surpassing those of monoculture cotton and jujube, particularly in 2023 when the yield increased by 55.35%. (3) A significant positive correlation was found between cotton yield and LAI. In conclusion, jujube–cotton intercropping enhances photosynthesis, improving yield, economic benefits, and land use efficiency. Full article
(This article belongs to the Special Issue Innovations in Green and Efficient Cotton Cultivation)
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10 pages, 2636 KiB  
Article
Low Temperature Characteristics of Ge-on-Si Waveguide Photodetectors: A Combined Simulation and Experimental Study
by Jingchuan Liu, Zhenyu Li, Xiaofei Liu, Wentao Yan, Xingyan Zhao, Shaonan Zheng, Yang Qiu, Qize Zhong, Yuan Dong and Ting Hu
Micromachines 2025, 16(5), 542; https://doi.org/10.3390/mi16050542 - 30 Apr 2025
Viewed by 572
Abstract
Benefiting from the progress of the germanium (Ge) epitaxy process on silicon (Si) substrates, waveguide-integrated Ge-on-Si photodetectors (PDs) have demonstrated decent performances in short-wave infrared (SWIR) detection. By lowering the operating temperature, theses PDs can meet the stringent signal-to-noise requirements for high-sensitivity detection. [...] Read more.
Benefiting from the progress of the germanium (Ge) epitaxy process on silicon (Si) substrates, waveguide-integrated Ge-on-Si photodetectors (PDs) have demonstrated decent performances in short-wave infrared (SWIR) detection. By lowering the operating temperature, theses PDs can meet the stringent signal-to-noise requirements for high-sensitivity detection. We systematically investigated the dark current characteristics and optical response in the 1500–1600 nm wavelength range of the waveguide-integrated Ge-on-Si PDs operated at low temperatures (200 K to 300 K). Under a −3 V bias, the PD exhibits a room-temperature dark current of 4.62 nA and a responsivity of 0.87 A/W at 1550 nm. When the temperature was reduced to 200 K, the dark current decreased to 93.69 pA, and the responsivity dropped to 0.34 A/W. Using finite-difference time-domain (FDTD) and technology computer-aided design (TCAD) simulations, we extracted the absorption coefficients of epitaxial Ge on Si at low temperatures. At room temperature, the absorption coefficient at the wavelength of 1550 nm was approximately 1100 cm−1, while at 200 K, the absorption coefficient decreased to 248 cm−1. The outcomes of this work pave the way for the high-performance low-temperature Si photonic systems in the future. Full article
(This article belongs to the Special Issue Research Progress of Silicon-Based Photodetectors)
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16 pages, 3853 KiB  
Article
Temporal Variation of Plankton Community in Typical Lake in Middle Reaches of Yangtze River: Structure, Environmental Response and Interactions
by Borui Zou, Hongjuan Hu, Jia Jia, Weiju Wu, Xin Li, Xiaofei Chen, Honghui Zeng, Zhi Wang and Chenxi Wu
Water 2025, 17(7), 1021; https://doi.org/10.3390/w17071021 - 31 Mar 2025
Viewed by 467
Abstract
Liangzi Lake, a typical shallow lake in the middle reaches of the Yangtze River, is important for water resource and biodiversity conservation. With the development of urbanization, anthropogenic activities have posed serious threats to the water quality and biodiversity of Liangzi Lake. To [...] Read more.
Liangzi Lake, a typical shallow lake in the middle reaches of the Yangtze River, is important for water resource and biodiversity conservation. With the development of urbanization, anthropogenic activities have posed serious threats to the water quality and biodiversity of Liangzi Lake. To assess the aquatic ecosystem health of Liangzi Lake, the structure, the environmental response, and the interactions of plankton were investigated in 2022 and 2023. The results indicated that water temperature was a pivotal factor regulating plankton dynamics, with the assemblage patterns predominantly shaped by the phytoplankton species, which were Bacillariophyta in spring and Chlorophyta in summer. In terms of the phytoplankton, dissolved oxygen and the N:P ratio significantly affect cyanobacteria distribution. The high biomass and abundance of cyanobacteria in summer highlight the potential risk of harmful algal blooms. In contrast to the phytoplankton, the zooplankton exhibited enhanced resilience to changes in the surrounding environment. Rotifera was the dominant group in summer in terms of both abundance and biomass. Most core genera of plankton were jointly identified by eDNA metabarcoding and microscopical analysis, and eDNA metabarcoding had advantages in revealing a higher diversity. However, some taxa among rotifers such as Liliferotrocha were only identified using microscopical analysis. Therefore, a combination of both the methods is recommended to better understand the structuring mechanisms of plankton assemblages in lake ecosystems. Full article
(This article belongs to the Section Biodiversity and Functionality of Aquatic Ecosystems)
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18 pages, 7859 KiB  
Article
Study on Sand-Steel Interface Shear Test Method and Mechanism
by Xiaofei Hu, Long Yu, Yunrui Han and Qing Yang
J. Mar. Sci. Eng. 2025, 13(3), 407; https://doi.org/10.3390/jmse13030407 - 22 Feb 2025
Viewed by 722
Abstract
Soil-structure interface properties play an essential role in geotechnical engineering. The interface shear test is widely used to measure the interface properties. However, in the traditional interface shear test (TIST), distribution of shear stresses along contact surface is not uniform due to boundary [...] Read more.
Soil-structure interface properties play an essential role in geotechnical engineering. The interface shear test is widely used to measure the interface properties. However, in the traditional interface shear test (TIST), distribution of shear stresses along contact surface is not uniform due to boundary effects. Thus, average mechanical response of the whole interface measured by TIST cannot be used to evaluate interface friction properties. In this paper, a novel interface shear apparatus (MIDST) is presented to investigate the shear behaviours of the soil-structure interface. A series of shear tests were conducted on Fujian standard sand-steel interface. Two shear force sensors simultaneously monitor the shear force along the interface: a pre-embedded sensor inside the interface/steel plate (responding to MIDST), while the other outside the interface (responding to TIST). Laboratory test results show that the pre-embedded internal sensor successfully detects the weakening characteristics of the interface, while the external sensor monitors the hardening law. The interface shear strength measured by internal sensor is significantly higher than that monitored by external sensor. A commercial DEM software Version 5.0, Particle Flow Code in Two Dimensions (PFC2D), is employed to study the soil-structure interaction mechanism, and numerical test results show that the main reasons for the internal and external differences are the uneven shear stress distribution at the soil-structure interface and the boundary effect. In addition, numerical test results agree with the laboratory test results, indicating that the shear behaviours monitored by MIDST are relatively accurate and can provide a reference for engineering design. Full article
(This article belongs to the Section Ocean Engineering)
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11 pages, 4041 KiB  
Article
High-Precision Field- Effect Transistor Biosensor for Analyzing Differential Effects of Anti-Cancer Drugs on Cancerous and Non-Cancerous Cells
by Can Li, Can Hu, Ling Li, Feng He and Xiaofei Li
Biosensors 2025, 15(2), 125; https://doi.org/10.3390/bios15020125 - 19 Feb 2025
Viewed by 920
Abstract
A high-precision biosensor technique is introduced, offering the capability to independently evaluate the effects of anti-cancer drugs on both cancerous (RAJI) and non-cancerous (WIL2S) cells. By analyzing and fitting current change curves and transfer characteristic curves under two drugs, camptothecin and doxorubicin, this [...] Read more.
A high-precision biosensor technique is introduced, offering the capability to independently evaluate the effects of anti-cancer drugs on both cancerous (RAJI) and non-cancerous (WIL2S) cells. By analyzing and fitting current change curves and transfer characteristic curves under two drugs, camptothecin and doxorubicin, this technique quantifies both the magnitude of drug-induced current changes in cells and the rate of drug entry into cells. Flow cytometry was utilized to validate the entry rates of two drugs, camptothecin and doxorubicin, into the cells. The biosensor leverages the exceptional sensitivity of two-dimensional electron gas to detect proximal charge variations at ultralow concentrations, even in fluids with high ionic strength. The findings reveal that anti-cancer drugs have a more pronounced impact on tumor cells, with the effects and interaction speeds differing across normal cells and tumor cells. This innovative approach not only enhances our understanding of the specificity and action mechanisms of anti-cancer drugs but also provides a valuable tool for screening potential tumor anti-cancer drugs and advancing targeted cancer therapies. Full article
(This article belongs to the Special Issue Innovative Strategies for Cancer Biosensing)
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15 pages, 21790 KiB  
Article
Research on the Corrosion Behavior of Mo/C276 Coating Deposited by HVOF Method in Deep-Sea Cold Seep Environments
by Pu Zhang, Hao Zhang, Wentao Hu, Yongjun Wang, Xiaofei Wu, Zhengwei Zhang, Zhihao Ren, Xu Zhai, Xian Zeng and Minggang Tang
Coatings 2025, 15(2), 194; https://doi.org/10.3390/coatings15020194 - 6 Feb 2025
Viewed by 688
Abstract
In this study, the Mo/C276 coating was deposited on Q690E steel by high velocity oxy-fuel spraying (HVOF), and the coating was treated with an organosilicon sealer. Further, the corrosion behaviors of the coating in the simulated deep-sea cold spring environment with hydrogen sulfide [...] Read more.
In this study, the Mo/C276 coating was deposited on Q690E steel by high velocity oxy-fuel spraying (HVOF), and the coating was treated with an organosilicon sealer. Further, the corrosion behaviors of the coating in the simulated deep-sea cold spring environment with hydrogen sulfide and high pressure were studied. The results show that when the oxygen flow rate is 220 NL/min, the coating has the lowest porosity of 1.71% and excellent mechanical properties. Combined with the micromorphology and elemental analysis of the coating, it was assumed that the Fe element generated by the corrosion of the Q690E substrate migrates to the surface of the coating. The corrosion tests in the simulated deep-sea cold showed that before the failure of the coating in the edge and corner areas, the corrosion rate of the coating was less than 0.002 mm/a, which could meet the long-term use requirements in the real cold spring environment. Full article
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14 pages, 4244 KiB  
Article
Impact of Grass Carp and Crucian Carp on Submerged Macrophyte and Phosphorus Cycling in Shallow Lake Mesocosms
by Xin Chen, Weiju Wu, Hongyi Ao, Shenghua Hu, Huaqiang Chen, Xiaofei Chen and Chenxi Wu
Water 2025, 17(3), 326; https://doi.org/10.3390/w17030326 - 24 Jan 2025
Cited by 1 | Viewed by 826
Abstract
Submerged macrophytes are essential for the restoration of shallow lakes for maintaining clear-water conditions. The presence of fish can affect the nutrient cycles and the growth of submerged macrophytes in lakes. In this study, a 28-day mesocosm experiment was carried out with an [...] Read more.
Submerged macrophytes are essential for the restoration of shallow lakes for maintaining clear-water conditions. The presence of fish can affect the nutrient cycles and the growth of submerged macrophytes in lakes. In this study, a 28-day mesocosm experiment was carried out with an herbivorous fish Ctenopharyngodon idella (CID) and an omni-benthivorous fish Carassius auratus (CAU) to investigate their effects on the growth of a submerged macrophyte Hydrilla verticillata and phosphorus (P) cycle in shallow lakes. The results showed that CID slowed down the growth of H. verticillata while CAU showed no significant effect. In overlying water, CID only increased the ammonium nitrogen (NH3-N) concentration in the later stage due to excretion, while CAU elevated particulate phosphorus (PP) levels during the experiment through disturbance. Meanwhile, the radial oxygen loss and photosynthesis of H. verticillata in CAU might promote the formation of NaOH-P and HCl-P in the sediment, respectively. Changes in the water and sediment properties caused by CID and CAU can contribute to the increase in the eutrophication risk index (ERI). Our findings suggest that CID has the potential to be an indirect biological manipulation tool, while CAU should be controlled to minimize its negative impacts on the P cycle in lakes. Full article
(This article belongs to the Section Biodiversity and Functionality of Aquatic Ecosystems)
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17 pages, 3743 KiB  
Article
Pegylated NIR Fluorophore-Conjugated OBHSA Prodrug for ERα-Targeted Theranostics with Enhanced Imaging and Long-Term Retention
by Xiaohua Wang, Xiaofei Deng, Lilan Xin, Chune Dong, Guoyuan Hu and Hai-Bing Zhou
Molecules 2025, 30(2), 305; https://doi.org/10.3390/molecules30020305 - 14 Jan 2025
Viewed by 1062
Abstract
In recent years, the near-infrared (NIR) fluorescence theranostic system has garnered increasing attention for its advantages in the simultaneous diagnosis- and imaging-guided delivery of therapeutic drugs. However, challenges such as strong background fluorescence signals and rapid metabolism have hindered the achievement of sufficient [...] Read more.
In recent years, the near-infrared (NIR) fluorescence theranostic system has garnered increasing attention for its advantages in the simultaneous diagnosis- and imaging-guided delivery of therapeutic drugs. However, challenges such as strong background fluorescence signals and rapid metabolism have hindered the achievement of sufficient contrast between tumors and surrounding tissues, limiting the system’s applicability. This study aims to integrate the pegylation strategy with a tumor microenvironment-responsive approach. A novel esterase-activated EPR strategy prodrug, OBHSA-PEG-DCM, was designed. This prodrug links OBHSA, a protein degrader capable of efficient ERα protein degradation, to the PEG-modified fluorescent group (dicyanomethylene-4H-pyran, DCM) via an ester bond. This integration facilitates targeted drug delivery and enhances the retention of the fluorescent group within the tumor, allowing distinct in vivo tumor imaging periods. Experimental results show that, benefiting from overexpressed esterase in cancer cells, OBHSA-PEG-DCM can be efficiently hydrolyzed, releasing OBHSA and pegylated DCM. OBHSA demonstrated potent inhibition against MCF-7 cells (IC50 = 1.09 μM). Simultaneously, pegylated DCM exhibited remarkable in vivo imaging capabilities, lasting up to 12 days in mice, due to the enhanced permeability and retention (EPR) effect. OBHSA-PEG-DCM holds promise as a theranostic agent for ERα-positive breast cancer, offering both therapeutic and diagnostic capabilities. Importantly, this study highlights the utility of pegylated NIR fluorophores for long-circulating drug delivery systems, addressing current challenges in achieving high-contrast tumor imaging and effective targeted drug release. Full article
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18 pages, 19589 KiB  
Article
Geological Conditions and Sedimentary Models of Oligocene and Eocene Effective Source Rocks in the Northern Yinggehai Basin
by Jianxiang Pei, Gaowei Hu, Zhipeng Huo, Zhihong Chen, Yabing Chen, Xiaofei Fu, Weihong Wang, Haiyu Liu, Yanan Wang, Jingshuang Luo and Guofei Chen
J. Mar. Sci. Eng. 2025, 13(1), 100; https://doi.org/10.3390/jmse13010100 - 7 Jan 2025
Viewed by 826
Abstract
The development of the effective source rocks of the Eocene and Oligocene directly determines the oil and gas exploration potential in the northern Yinggehai Basin in China. Based on the analogy with the Hanoi Depression in Vietnam and the Yacheng District in the [...] Read more.
The development of the effective source rocks of the Eocene and Oligocene directly determines the oil and gas exploration potential in the northern Yinggehai Basin in China. Based on the analogy with the Hanoi Depression in Vietnam and the Yacheng District in the Qiongdongnan Basin and the comprehensive analysis of self-geological conditions, the development conditions of Eocene and Oligocene source rocks in the northern Yinggehai Basin are examined, focusing on tectonic evolution, sedimentary facies, and the paleoenvironment. Finally, the sedimentary models for the effective source rocks are established. The tectonic activity controlled the formation of the sedimentary deep depression and the migration of the sedimentary trough center, which migrated from east to west and then south from the Eocene to the Oligocene, leading to the sedimentary migration of good muddy source rocks. There are multiple sedimentary facies in favor of source rocks, including lacustrine facies, shallow marine facies, and delta plain swamps. The paleoenvironment indicates that the paleoclimate transitioned from warm and humid to cold and arid, the redox conditions evolved from semi-reducing to oxic, and paleoproductivity increased from the early to late Oligocene. Therefore, the early Oligocene was more conducive to the enrichment of organic matter. It is speculated that the warm and humid paleoclimate, reducing environment, and high paleoproductivity of the Eocene promoted the sedimentation and preservation of more organic matter. The above studies show that the northern Yinggehai Basin, especially the sedimentary period of the Eocene and Oligocene, has favorable geological conditions for the development of effective source rocks. The sedimentary models for Eocene lacustrine mudstones and Oligocene marine mudstones and marine–continental transitional coal-measure source rocks were established. These studies make up for the serious deficiency of previous research and mean that there is great exploration potential for oil and gas in the northern Yinggehai Basin in China. Full article
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20 pages, 15749 KiB  
Article
Study on the Vibration Propagation Law and Stress Distribution Characteristics in Double-Arch Tunnels During Blasting
by Xiaofei Sun, Ying Su, Dunwen Liu, Yu Tang, Pei Zhang, Jishuang Hu and Xianghao Sun
Buildings 2025, 15(1), 139; https://doi.org/10.3390/buildings15010139 - 5 Jan 2025
Cited by 1 | Viewed by 1133
Abstract
Highway tunnel construction in mountainous areas of China has been developing rapidly. The influence of drilling and blasting on the existing tunnel structure has become a key factor affecting the safety and stability of tunnel construction. The double-arch tunnel has unique structural characteristics. [...] Read more.
Highway tunnel construction in mountainous areas of China has been developing rapidly. The influence of drilling and blasting on the existing tunnel structure has become a key factor affecting the safety and stability of tunnel construction. The double-arch tunnel has unique structural characteristics. The propagation characteristics of blasting vibrations and the resulting stress responses exhibit a certain level of complexity. There is little research on the influence of single-line blasting excavation of double-arch tunnel on the other line tunnel. This paper analyzes the blasting vibration of a double-arch tunnel by ANSYS/LS-DYNA. The propagation law of blasting vibration velocity and stress distribution law of blasting vibration in different sections of the tunnel is revealed. At the same time, the relationship between the peak particle velocity (PPV) and tensile stress is established, and the threshold vibration velocity is proposed. It provides a scientific basis for tunnel design and construction. The propagation of blasting vibration in the adjacent roadway is affected by the middle pilot tunnel. The peak vibration velocity of different parts decreases with the increase in distance. The monitoring of vibration velocity and stress in section A of the right line of the adjacent tunnel should be strengthened, especially in the tunnel vault, blast-facing side wall, and arch foot. The difference in vibration strength across different tunnel parts provides a basis for optimizing the structure. It helps strengthen the parts susceptible to vibration during the design stage of the multi-arch tunnel, improving the tunnel’s safety and stability. Full article
(This article belongs to the Special Issue Dynamic Response of Civil Engineering Structures under Seismic Loads)
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12 pages, 3048 KiB  
Article
Development of an Enzyme-Linked Immunosorbent Assay Based on a Monoclonal Antibody for the Rapid Detection of Citrinin in Wine
by Xingdong Yang, Yang Qu, Chenchen Wang, Lihua Wu and Xiaofei Hu
Foods 2025, 14(1), 27; https://doi.org/10.3390/foods14010027 - 25 Dec 2024
Cited by 2 | Viewed by 866
Abstract
The ingestion of food contaminated with citrinin (CIT) poses a variety of health risks to humans and animals. The immunogens (CIT-COOH-BSA, CIT-H-BSA) and detection antigen (CIT-COOH-OVA, CIT-H-OVA) were synthesised using the active ester method (-COOH) and formaldehyde addition method (-H). A hybridoma cell [...] Read more.
The ingestion of food contaminated with citrinin (CIT) poses a variety of health risks to humans and animals. The immunogens (CIT-COOH-BSA, CIT-H-BSA) and detection antigen (CIT-COOH-OVA, CIT-H-OVA) were synthesised using the active ester method (-COOH) and formaldehyde addition method (-H). A hybridoma cell line (3G5) that secretes anti-CIT monoclonal antibodies (mAbs) was screened via CIT-H-BSA immunisation of mice, cell fusion, and ELISA screening technology. The cell line was injected intraperitoneally to prepare ascites. The reaction conditions for the indirect competitive ELISA (ic-ELISA) were optimised, and an ic-ELISA method for detecting CIT was preliminarily established. The results revealed that the IC50 of CIT from optimised ic-ELISA was 37 pg/mL, the linear detection range was 5.9~230 pg/mL, and the cross-reaction (CR) rate with other analogues was less than 0.01%. The intra-assay and interassay sample recovery rates of CIT were 84.7~92.0% and 83.6~91.6%, and the coefficients of variation (CVs) were less than 10%. The ic-ELISA of CIT established in this study was not significantly different from the HPLC results and is rapid, highly sensitive and strongly specific, providing technical support for the detection of CIT. Full article
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17 pages, 4141 KiB  
Article
Understory Vegetation Preservation Offsets the Decline in Soil Organic Carbon Stock Caused by Aboveground Litter Removal in a Subtropical Chinese Fir Plantation
by Bingshi Xu, Fangchao Wang, Kuan Liang, Ren Liu, Xiaofei Hu, Huimin Wang, Fusheng Chen and Mingquan Yu
Forests 2024, 15(12), 2204; https://doi.org/10.3390/f15122204 - 14 Dec 2024
Viewed by 1038
Abstract
Forest soils play a key role in the global carbon (C) pool and in mitigating climate change. The mechanisms by which understory and litter management affect soil organic C (SOC) concentrations are unclear in subtropical forests. We collected soils along a 60 cm [...] Read more.
Forest soils play a key role in the global carbon (C) pool and in mitigating climate change. The mechanisms by which understory and litter management affect soil organic C (SOC) concentrations are unclear in subtropical forests. We collected soils along a 60 cm profile in a Chinese fir (Cunninghamia lanceolata) plantation treated by only aboveground litter removal and understory vegetation preservation (Only-ALR), both aboveground litter and understory vegetation removal (ALR+UVR), and both aboveground litter and understory vegetation preservation (control) for 7 consecutive years. Five SOC fractions, physico-chemical properties, the biomass of microbial communities and the activities of C-acquiring enzymes were measured, and their correlations were analyzed for each of four soil layers (0–10, 10–20, 20–40 and 40–60 cm). Compared with control, Only-ALR decreased labile C pool I (LP-C I), labile C pool II (LP-C II) and dissolved organic C (DOC) in topsoil (0–20 cm) but had no effect on soil C fractions in subsoil (20–60 cm). A higher fungi and bacteria biomass in LP-C II and microbial biomass C (MBC) stock was observed in Only-ALR compared to ALR+UVR treatment. Soil pH and Gram-positive bacteria generally had impact on the variation of soil C fractions in topsoil and subsoil, respectively. Understory vegetation preservation offsets the declines of SOC and recalcitrant C but not the decreases in labile C caused by aboveground litter removal. Understory vegetation helps sustain SOC stock mainly via decreased C input and elevated soil pH which would change microbial biomass and activities when litter is removed. Our findings highlight the potential influence of long-term understory manipulation practices on C pool within a soil profile in subtropical plantation forests. Full article
(This article belongs to the Special Issue Carbon, Nitrogen, and Phosphorus Storage and Cycling in Forest Soil)
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