Search Results (97)

The white-spotted jellyfish, Phyllorhiza punctata, is an invasive species with significant ecological and economic relevance spreading across various regions. While its ecological impact is well-documented, its molecular and biochemical characteristics remain poorly understood. In this study, we integrate proteomic data generated by LC-MS/MS with publicly available transcriptomic information to characterize P. punctata, analyzing differential protein expression across three distinct tissues: oral arms, mantle, and gonads. A total of 2764 proteins and 25,045 peptides were identified, including several venom components such as jellyfish toxins (JFTs) and phospholipase A2 (PLA2), which were further investigated and compared to toxins from other species. Enrichment analyses revealed clear tissue-specific functions. Additionally, deep learning and machine learning tools identified 274 promising AMP candidates, including the α-helical, β-sheet, and αβ-motif peptides. This dataset provides new insights into the protein composition of P. punctata and highlights strong AMP candidates for further characterization, underscoring the biotechnological potential of underexplored cnidarian species. Full article

Background: The aim of this study was to analyze levels of frailty, across physical, psychological, social, and overall dimensions, according to marital status, age, number of diagnosed illnesses, and number of medications taken in community-dwelling older women. Methods: The study included a total of 94 older women, aged between 60 and 89 years. All participants completed a sociodemographic and clinical questionnaire, as well as an instrument to measure physical, psychological, and social frailty, along with the total frailty score in the study participants. Group comparison test, such as the Kruskal–Wallis test, was applied. Results: Statistically significant differences (p < 0.05) in frailty were associated with marital status, clinical burden, and polypharmacy, with widowed/divorced individuals, those with more diagnosed diseases, and those taking more medications exhibiting higher physical and total frailty levels, while psychological frailty was notably higher in the oldest age group. Conclusions: The results indicate that physical and social interventions should be more relevant for older women with greater social isolation, as they may increase frailty indices and consequently the risk of hospitalization, institutionalization, and mortality. Full article

Endothelial inflammation and atherosclerosis remain leading drivers of cardiovascular disease, yet the post-transcriptional regulators orchestrating these events are not yet completely understood. In this review, we analyse recent preclinical and clinical studies to dissect microRNA (miRNA)-mediated control of vascular endothelial biology. We describe how miR-181b-5p and miR-223 modulate NLRP3 inflammasome activation and pyroptosis, how miR-615-5p, miR-138, and miR-133a coordinate endothelial nitric oxide synthase (eNOS) activity and nitric oxide bioavailability, and how miR-33a/b, miR-150, and miR-342-3p influence lipid efflux and foam-cell formation in atherogenesis. We also discuss miRNA signatures that correlate with endothelial dysfunction in human cohorts. By integrating mechanistic pathways with emerging biomarker data, this study underscores the relevance of miRNAs as both diagnostic and potential targets in vascular diseases. Full article

Magnesium-based coronary stents have gained significant interest due to their excellent biocompatibility, biodegradability, and mechanical properties. However, a key limitation of magnesium in biomedical applications is its low corrosion resistance, which compromises its structural integrity and mechanical strength over time. Polymeric coatings can overcome this challenge, enhancing magnesium-based implants’ corrosion resistance and overall performance. This study applied a polylactic acid (PLA) nanofiber coating to WE43 magnesium (Mg) stents via electrospinning to reduce their corrosion rate. Both uncoated and coated stents underwent in vitro immersion tests in Hank’s solution for 1, 3, 7, and 14 days. The effectiveness of the PLA coating was evaluated through morphological analysis, chemical composition assessment, corrosion behavior (weight change), magnesium ion release, and in vitro biocompatibility. The corrosion observed in the uncoated WE43 stents indicates that protective coatings are necessary to regulate degradation rates over extended implantation periods. The results demonstrated that coated stents exhibited improved performance, maintaining the integrity of the PLA coating for up to 14 days. The coated stents demonstrated reduced surface damage and lower weight loss resulting from lower magnesium release. In our study, the coated stents demonstrated a reduced corrosion rate (0.216 ± 0.013 mm/year) compared with the uncoated stents (0.312 ± 0.010 mm/year), both after 14 days. Additionally, in vitro biocompatibility results confirmed the non-toxic nature of PLA-coated stents, which enhances cellular proliferation and contributes to a more favorable environment for vascular healing. These findings suggest that PLA coatings can effectively prolong the functional durability of WE43 Mg stents, offering a promising solution for enhancing the performance of biodegradable stents in cardiovascular applications. Full article

The COVID-19 pandemic disrupted tuberculosis (TB) control, increasing mortality and potentially worsening disparities. This study aimed to analyze the temporal trends of TB mortality in Brazil and to trace the COVID-19 pandemic’s effect using a Bayesian approach, focusing on nationwide data. An ecological study of TB deaths recorded in the Mortality Information System (SIM) from 2012 to 2022 was conducted. Trends and percentage changes in the mortality were estimated. A Bayesian Structural Time Series model combined with an Autoregressive Integrated Moving Average model was used to assess the pandemic’s effect on TB. A total of 51,809 TB deaths were identified, with a mortality rate of 2.27 per 100,000. Higher rates were found among the elderly (6.86), indigenous populations (5.58), and black individuals (4.21). The Bayesian model estimated a 9.9% (CI 8.8–11%) increase in TB mortality due to COVID-19. The Midwest region showed the highest increase (30%, 25–35%). Females experienced a greater post-pandemic monthly increase (2.80%) in mortality than males (0.72%). The Bayesian analysis revealed a significant rise in TB mortality during the COVID-19 pandemic, with notable disparities affecting females, the elderly, the indigenous, and the black populations. These findings highlight the pandemic’s long-term impact on TB and stress the need for equity-focused, data-driven public health responses in Brazil. Full article

Among the highly toxic heavy metals, cadmium (Cd) is highlighted as a persistent environmental pollutant, posing serious threats to plants and broader ecological systems. Phytochelatins (PCs), which are synthesized by phytochelatin synthase (PCS), are peptides that play a central role in Cd mitigation through metal chelation and vacuolar sequestration upon formation of Cd-PC complexes. PC synthesis interacts with other cellular mechanisms to shape detoxification outcomes, broadening the functional scope of PCs beyond classical stress responses. Plant Cd-related processes have has been extensively investigated within this context. This perspective article presents key highlights of the panorama concerning strategies targeting the PC pathway and PC synthesis to manipulate Cd-exposed plants. It discusses multiple advances on the topic related to genetic manipulation, including the use of mutants and transgenics, which also covers gene overexpression, PCS-deficient and PCS-overexpressing plants, and synthetic PC analogs. A complementary bibliometric analysis reveals emerging trends and reinforces the need for interdisciplinary integration and precision in genetic engineering. Future directions include the design of multigene circuits and grafting-based innovations to optimize Cd sequestration and regulate its accumulation in plant tissues, supporting both phytoremediation efforts and food safety in contaminated agricultural environments. Full article

Background: A comprehensive understanding of variations in physical attributes both between and within young players is crucial for accurately identifying talent based on physical performance. This study aimed to compare maximum strength, jump, and sprint test results among young soccer players from different age categories and playing positions. Secondarily, this study aimed to analyze the association between maximum strength, jump, and sprint performances. Methods: A total of 103 players were categorized as U23, U19, U17, and U16. The players were placed into these age categories based on their football abilities. All participants completed standardized warm-ups, and testing procedures followed protocols established in previous studies. Results: Significant differences were found between age categories regarding the Isometric Mid-Thigh Pull (p < 0.001; η²_p = 0.33), Countermovement Jump (CMJ) (p < 0.001; η²_p = 0.50), Squat Jump (SJ) (p < 0.001; η²_p = 0.29), and sprint (p < 0.001; η²_p = 0.30) tests. No significant differences were detected in Broad Jump results between age categories. Moreover, no significant differences were observed in any physical capacities between playing positions. Furthermore, significant moderate-to-strong correlations (r = 0.30–0.86) were observed between all physical tests. Conclusions: Age categories can distinguish soccer players’ performance in different physical tests while no differences were observed between playing positions. Similar magnitude correlations were observed across all tests with only the CMJ and SJ being strongly correlated. Coaches and strength and conditioning professionals should apply a variety of tests to assess different physical qualities since they have different patterns between age categories. Full article

Bats and their ectoparasites play a crucial role in understanding the ecology and transmission of vector-borne pathogens, yet these dynamics remain poorly studied in Portugal. This study aimed to investigate the molecular occurrence of vector-borne bacteria (Anaplasma spp., Bartonella spp., Ehrlichia spp., and Rickettsia spp.) and protozoa (Babesia spp. and Theileria spp.) in ectoparasites of cave-dwelling bats. Bats were sampled from two caves in Portugal, and their ectoparasites included wing mites (Spinturnix myoti), ticks (Ixodes simplex), and bat flies (Penicillidia conspicua and Nycteribia schmidlii). Molecular analyses revealed the presence of Bartonella spp. in S. myoti and N. schmidlii. Phylogenetic inference based on the gltA gene positioned the detected genotypes close to those previously reported in bats and Nycteribiidae flies in Europe, Asia, and Africa. Notably, no DNA from Anaplasmataceae, Rickettsia spp., or piroplasmids was detected. The prevalence of S. myoti was high, with all examined bats being infested, showing notable differences in ectoparasite diversity concerning sex and cave-specific location. These findings suggest that host behavior, environmental conditions, and ectoparasite lifecycles play critical roles in shaping pathogen transmission dynamics. This study advances the understanding of bat ectoparasite–pathogen interactions in a region with limited data and highlights the need for continued research to assess the zoonotic potential and ecological impacts of the Bartonella genotypes detected herein. Full article

Sea anemones (Actiniaria, Cnidaria) are promising targets for biomedical research, as they produce unique bioactive compounds, including toxins and antimicrobial peptides (AMPs). However, the diversity and mechanisms underlying their chemical defenses remain poorly understood. In this study, we investigate the proteomic profiles of the unexplored sea anemone Actinia fragacea by analyzing its venom nematocyst extract, tissues, and mucus secretion. A total of 4011 different proteins were identified, clustered into 3383 protein groups. Among the 83 putative toxins detected, actinoporins, neurotoxins, and phospholipase A2 were uncovered, as well as two novel zinc metalloproteinases with two specific domains (ShK) associated with potassium channel inhibition. Common Gene Ontology (GO) terms were related to immune responses, cell adhesion, protease inhibition, and tissue regeneration. Furthermore, 1406 of the 13,276 distinct peptides identified were predicted as potential AMPs, including a putative Aurelin-like AMP localized within the nematocysts. This discovery highlights and strengthens the evidence for a cnidarian-exclusive Aurelin peptide family. Several other bioactive compounds with distinctive defense functions were also detected, including enzymes, pattern recognition proteins (PRPs), and neuropeptides. This study provides the first proteome map of A. fragacea, offering a critical foundation for exploring novel bioactive compounds and valuable insights into its molecular complexity. Full article

Overcoming the growing challenge of antimicrobial resistance (AMR), which affects millions of people worldwide, has driven attention for the exploration of marine-derived antimicrobial peptides (AMPs) for innovative solutions. Cnidarians, such as corals, sea anemones, and jellyfish, are a promising valuable resource of these bioactive peptides due to their robust innate immune systems yet are still poorly explored. Hence, we employed an in silico proteolysis strategy to search for novel AMPs from omics data of 111 Cnidaria species. Millions of peptides were retrieved and screened using shallow- and deep-learning models, prioritizing AMPs with a reduced toxicity and with a structural distinctiveness from characterized AMPs. After complex network analysis, a final dataset of 3130 Cnidaria singular non-haemolytic and non-toxic AMPs were identified. Such unique AMPs were mined for their putative antibacterial activity, revealing 20 favourable candidates for in vitro testing against important ESKAPEE pathogens, offering potential new avenues for antibiotic development. Full article

Tomato (Solanum lycopersicum), which is considered one of the more important and widely cultivated crop members of the family Solanaceae, exhibits numerous biochemical mechanisms to alleviate the stress produced by various biotic and abiotic factors. Many researchers have found that phytochelatins (PCs) play an important role in these stress-alleviating mechanisms and, therefore, contribute significantly to the plant’s coping strategies, particularly under heavy metal exposure. Ongoing research has extensively investigated tomato genotypes in plant stress research, with a particular focus on heavy metal stress. The production of PCs, synthesized from glutathione, is regulated by various factors and different stressors. Here, we aim to provide an overview of the panorama regarding the synthesis of PCs in tomato under different environmental conditions and experimental settings, as well as provide information on their broader roles in biotechnology and modulating plant tolerance and responses across diverse stress conditions and treatments within the context of tomato research. Full article

The stop-hole technique is a well-known strategy to extend the fatigue life of cracked components. The ability to estimate fatigue life after the hole is important for safety reasons. The objective here is to develop strategies for the accurate prediction of initiation and propagation life ahead of the stop-hole. Experimental work was developed in a Compact-Tension (CT) specimen made of 7050-T7451 aluminium alloy and with a 3 mm diameter hole. A total number of 625,000 load cycles were required to re-initiate the crack after the hole. Crack initiation life after the hole was estimated using the Theory of Critical Distances combined with the Smith–Watson–Topper parameter. A value of a₀ = 31.83 µm was obtained for El Haddad parameter, which was used to define the critical distance. The predicted life was found to be only 4% lower than the experimental value. The fatigue crack growth (FCG) rate was calculated using a node release strategy, assuming that cyclic plastic deformation is the main damage mechanism and that cumulative plastic strain is the crack driving parameter. A good agreement was found between the numerical predictions of da/dN and the experimental results. The main result, however, is the proposed methodology, which allows predicting the initiation and propagation lives in notched components. Full article

Introduction: Vaccines are a significant public health achievement, which are crucial for child survival and disease control globally. In Brazil, the National Immunization Program (PNI) manages vaccination schedules, including essential vaccines like BCG and Hepatitis B, administered at birth. Despite achieving over 95% coverage for years, vaccination rates have declined since 2016, a trend exacerbated by the COVID-19 pandemic. This study aims to analyze spatial and temporal trends in BCG and Hepatitis B vaccination coverage at birth, identify areas with spatial variation in these trends, classify the identified trends, and investigate the pandemic’s impact on vaccination adherence. Methods: This is an ecological study with real-world data from Brazil, focusing on vaccination coverage from 2014 to 2023. Utilizing the Spatial Variation in Temporal Trends (SVTT) technique, the study identifies municipalities’ vaccination trends. It also employs time series analysis and Interrupted Time Series methods to evaluate the pandemic’s impact on vaccination rates, using data from the PNI and the Information System on Live Births (SINASC). Results: Between January 2014 and December 2023, Brazil administered 25,902,207 doses of the BCG vaccine to children at birth, with 3911 municipalities (70.24%) showing declining trends, particularly in Florianópolis. Similarly, 22,962,434 doses of the Hepatitis B vaccine were administered, with 3284 municipalities also experiencing declines. Conclusions: It is crucial that public health policies be reevaluated to address regional disparities in vaccination coverage, particularly in more vulnerable areas. Focused interventions, such as awareness campaigns, improved access to vaccination services, and strengthened monitoring, are fundamental to reversing this trend. Full article

With the increasing concern on heavy metal contamination in agriculture and other environmental settings, unraveling the mechanisms of cadmium (Cd) tolerance and response in plants has become highly important. Ongoing plant Cd research over the years has focused on strategic and relevant aspects, including molecular, biochemical, and physiological processes. From this perspective, phosphoproteomics appears to be an innovative and powerful approach to investigating plant responses to Cd stress. Here, we summarize progress in plant Cd research across different plant species regarding large-scale phosphoproteomic investigations. Some studies revealed major proteins participating in detoxification, stress signaling, and metabolism, along with their regulation through phosphorylation, which modulates the plant’s defense against Cd. However, many pathways remain unexplored. Expanding these studies will help our ability to alleviate Cd stress and provide further information concerning involved mechanisms. Our purpose is to inspire researchers to further explore the use of phosphoproteomics in unraveling such complex mechanisms of Cd tolerance and response across various plant species, with the ultimate aim of enhancing strategies for mitigating Cd stress in agriculture and polluted environments. Full article

By-products generated in the winemaking industry contain compounds with health-promoting properties, which can be reintroduced into the food production chain. This study evaluated the use of a by-product from the industrial processing of grapes as an ingredient in the manufacture of Petit Suisse cheese, made with A2A2 milk and the addition of the probiotic Bifidobacterium animalis subsp. lactis HN019. Two Petit Suisse formulations were made in three independent batches: a control formulation without the addition of the by-product (F0) and a formulation containing 10% of the by-product (F1). The proximate composition of the cheeses was characterized on the first day after manufacturing them. The addition of the by-product led to an increase in ash, lipids, and carbohydrates and a reduction in moisture and protein contents. The physicochemical characterization and the texture profile analysis showed no changes throughout the product’s shelf life. The probiotic counts remained abundant (~eight log CFU/g) in both formulations with no changes seen throughout the shelf life period. Scanning electron microscopy images showed the added bacteria had typical structures. No differences were observed in the fatty acid profiles of the formulations, and both exhibited a total of 18 fatty acids, including saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs). Additionally, the by-product conferred antioxidant activity to the F1 formulation. The addition of the by-product in fresh cheese may be an interesting approach in regards to the processing technology used, its microbiological safety, and its nutritional value. The use of A2A2 milk and a probiotic culture thus enhanced the Petit Suisse cheese, resulting in a healthier product. Full article