Search Results (836)

Congenital toxoplasmosis, caused by the obligatory intracellular apicomplexan protozoan Toxoplasma gondii, can lead to severe complications during pregnancy, including fetal malformations and spontaneous abortion. In the present study, the anti-T. gondii effects of the natural flavonoid quercetin were evaluated using in vitro, ex vivo, and in silico models. Cell viability and intracellular proliferation of the parasite were determined via colorimetric assays. The lipid droplet assay was analyzed using Nile Red staining, the antioxidant and oxidative stress parameters were determined by biochemical assays, and the cytokine levels were quantified by immunoassays. Our results demonstrated that non-cytotoxic concentrations of quercetin (CC₅₀ > 100 μM) significantly inhibited parasite proliferation (IC₅₀ = 14.10 ± 2.83 μM; SI > 7.09) in an irreversible manner. Quercetin impairs parasite adhesion, invasion, and reinfection capacity. In parallel, quercetin reduced lipid droplet accumulation, restored antioxidant balance by modulating redox biomarkers, and regulated cytokine production, notably increasing IL-4, IL-6, and IL-8 levels. Corroborating the in vitro findings, quercetin significantly reduced T. gondii proliferation in human placental villous explants while preserving tissue architecture and viability. In silico analyses revealed that quercetin binds to the active site of T. gondii hypoxanthine-guanine phosphoribosyltransferase (TgHGPRT) and exhibits favorable pharmacokinetic and drug-likeness properties. Full article

Nanotechnology has emerged as a promising strategy in restorative dentistry and endodontics due to the physicochemical and biological properties of nanomaterials. This narrative review aimed to critically analyze the current applications of nanomaterials in restorative dentistry and endodontics, highlighting their mechanisms of action, biological properties, and translational potential. A literature search was performed in the PubMed/MEDLINE database using combinations of MeSH terms and free keywords related to nanomaterials and dental applications. Studies published in English within the last twenty years and addressing restorative or endodontic applications were considered. After screening and eligibility assessment, 69 studies were included in the descriptive analysis. The findings indicate that nanomaterials have been investigated in preventive strategies, adhesive systems, restorative materials, intracanal medicaments, endodontic sealers, vital pulp therapy, and regenerative formulations. In restorative dentistry, nanoparticles such as silver nanoparticles, nano-hydroxyapatite, amorphous calcium phosphate, bioactive glass nanoparticles, and chitosan-based systems showed favorable antimicrobial, remineralizing, and material-enhancing properties. In endodontics, silver and chitosan nanoparticles showed potential for intracanal disinfection and biofilm disruption, while chlorhexidine, zinc, and bioactive glass nanoparticles enhanced the antimicrobial activity and sealing ability of endodontic sealers. In addition, magnetic nanoparticles, mesoporous silica nanoparticles, and hydroxyapatite nanoparticles presented promising applications in regenerative endodontics and vital pulp therapy. However, most of the available evidence is still based on in vitro studies, with limited long-term clinical validation. Overall, nanotechnology offers potential experimental advantages for improving preventive, restorative, and endodontic therapies; however, its successful clinical translation remains strictly dependent on overcoming critical biosafety barriers and addressing long-term toxicity concerns. Full article

Vacuolar Protein Sorting 29 (VPS29) is a highly conserved subunit of the retromer complex, which mediates retrograde transport from endosomes to the Golgi apparatus and plays a critical role in membrane trafficking, protein recycling, and organelle biogenesis. In Plasmodium falciparum, the retromer has been implicated in the formation of apical organelles essential for parasite invasion and replication. In this study, we investigated naturally acquired antibody responses to P. falciparum VPS29 (PfVPS29) and the genetic diversity of the vps29 gene in isolates from three malaria-endemic areas of the Brazilian Amazon. Naturally acquired responses to PfVPS29 were evaluated by ELISA in 533 individuals, and genetic diversity was assessed in 62 P. falciparum isolates. Only 17% of participants displayed IgG reactivity, whereas 73.5% showed IgM responses, indicating limited IgG acquisition but a predominant IgM profile associated with recent or ongoing exposure. IgG subclass analysis revealed a predominance of cytophilic IgG1 and IgG3 among responders. IgM responses were significantly boosted during P. falciparum infection. Sequence analysis revealed no polymorphisms among Brazilian isolates, and comparison with global datasets confirmed the high conservation of the PfVPS29 coding sequence. Together, these findings show that PfVPS29 is a highly conserved intracellular protein that elicits an atypical humoral response dominated by IgM, with limited class switching to IgG, like other conserved or repetitive malaria antigens. These results highlight PfVPS29 as an example of a conserved intracellular antigen that induces non-classical humoral responses in naturally exposed populations. Full article

Objective: To map studies that evaluated national policy documents aimed at promoting physical activity and/or reducing sedentary behavior in South American countries. Methods: A scoping review was conducted, including complete studies that addressed national public policy documents for the promotion of physical activity and reduction of sedentary behavior in any South American country. Eligible studies were those obtained from electronic databases, without restrictions on language or year of publication. The search began with the equation: policy AND (“physical activity” OR “sedentary behavior” OR “screen time”) AND “health” AND (evaluat* OR assess*). Results: Thirteen studies were identified, which allowed us to identify that research conducted in South America has not yet included the analysis of public policy documents aimed at promoting the reduction of sedentary behavior in the subcontinent’s population. The majority of these studies were published in the last four years, and most analyzed Brazilian public policy documents. While some documents from intersectoral initiatives were found, the majority refer to national health sector programs focused on children and adolescents. Conclusion: In addition to helping identify the main characteristics of South American studies analyzing public policy documents on physical activity and sedentary behavior, these findings highlight how little this topic is still explored in the South American subcontinent. Full article

Windthrow and the associated damage to forests have significant economic, social, and ecological impacts including increased harvesting costs and lost revenue, safety concerns for forest workers, and restriction on public access. The impacts of wind damage also directly affect greenhouse gas profiles associated with forest lands. This paper describes a two-stage forest monitoring approach that was devised for the purposes of assessing the impacts of the storms of winter 2024/2025, which included Storms Darragh and Éowyn, on the Irish forest estate. A range of Earth Observation (EO) datasets were used to assess the extent of windthrow damage within both public and private forests across the Republic of Ireland. The total area damaged was ca. 27,400 ha out of a total forest area of ca. 800,000 ha mainly affecting the north-west of the country. Based on scenarios developed to analyse the level of harvest in conjunction with the salvage operations, it was found that there was a decline in the sink capacity of the forest estate over the period 2025–2030. However, beyond this period, the sink capacity is restored as a result of the regeneration of the forests. Full article

This research proposes a physics-based deep learning framework, developed as a proof-of-concept based on synthetic data, for estimating the mechanical properties of layered media—namely density (ρ), Young’s modulus (E), and top layer thickness (h₁)—using synthetic seismogram images generated via Finite Element Method (FEM) simulations. The dataset, comprising 5000 simulations, incorporates physical constraints and empirical density–modulus correlations. While a ResNet-style Convolutional Neural Network (CNN) extracts density and stiffness parameters from composite time–frequency images, the estimation of h1 utilizes a direct time-domain raw-signal approach to preserve spatial resolution. A 5-fold nested cross-validation scheme with internal Bayesian Optimization ensures rigorous model evaluation, further validated by normality assessments and bootstrap confidence intervals. Performance was tested against synthetic Gaussian noise (0% to 50%) and benchmarked against classical Full Waveform Inversion (FWI). The results demonstrate high predictive accuracy for shallow properties, with R² values reaching 0.96 for Young’s modulus and 0.83 for raw-signal thickness. The neural network model requires 0.035 s per inference compared to 180 s for the FWI approach, avoiding the local minima convergence issues typical of iterative inversion. The framework exhibits resilience under moderate noise levels (up to 30%), establishing a reliable baseline for future experimental validation. Full article

The selection of an appropriate fixation system is critical for maintaining postoperative stability after sagittal split ramus osteotomy (SSRO), especially in cases involving large mandibular advancements and counterclockwise rotation, where mechanical stresses may compromise treatment outcomes. This in vitro study evaluated the biomechanical stability of five fixation systems following sagittal split ramus osteotomy (SSRO) under two mandibular advancement conditions. Fifty polyurethane hemimandibles were allocated into two experimental groups: Group 1, submitted to 10-mm linear advancement, and Group 2, submitted to 10-mm advancement associated with 20° counterclockwise rotation. Each group was further divided into five subgroups according to the fixation design employed: (A) conventional straight plate, (B) angled plate, (C) sagittal plate, (D) 10-hole miniplate, and (E) two 4-hole miniplates. Biomechanical performance was assessed by compression testing using a universal testing machine. Complementary analyses were performed using strain gauges and digital image correlation. Group 1 in fixation B, C and D demonstrated higher biomechanical resistance than Group 2, however without significance difference. Among the evaluated configurations, subgroup E exhibited the highest resistance values in both experimental conditions. Statistically significant differences were observed among fixation systems within each group (p < 0.05). The fixation systems using two miniplates demonstrated superior biomechanical stability, particularly in mandibular advancements associated with counterclockwise rotational movements. These findings suggest that this fixation strategy may contribute to enhanced postoperative stability in orthognathic surgery involving large mandibular advancements and complex rotational movements. Full article

This work proposes a multiscale spatial and temporal approach to assess the impacts of the ionosphere and neutrosphere (neutral atmosphere including both tropospheric and stratospheric) through an independent analysis of each component on Precise Point Positioning (PPP) accuracy and stability during selected representative geomagnetic events of Solar Cycle 25. Geomagnetically quiet and disturbed days were selected using the Kp index, with 21 multi-GNSS stations distributed across latitude bands. Kinematic PPP processing was performed using APPPOLO software (v1.0) with ionosphere-free dual-frequency combinations, precise products, and robust filtering, totaling 924 solutions. Results show improvements in geometry and satellite availability with multi-GNSS, achieving discrepancies within 0–10 cm in more than 89% of the solutions. The VMF3 model confirmed the deterministic behavior of ZHD and the latitudinal variability of ZWD, with increased stability in multi-GNSS solutions. Greater degradation was observed at high latitudes under disturbed geomagnetic conditions, particularly for GPS-only processing. Residual analysis indicated elevation-dependent effects and constellation-related differences. The analysis of ionospheric irregularities using ROTI revealed that PPP degradation is strongly associated with spatial distribution and satellite geometry, with enhanced effects at high latitudes and low elevation angles. Full article

As one of the most economically important cucurbit crops, melon (Cucumis melo L.) is extensively cultivated in semi-arid and tropical regions where high evaporative demand frequently intensifies salt accumulation. These conditions promote the evaporative concentration of salt, leading to salt accumulation in soil and irrigation water, which can impair crop development. Therefore, identifying approaches capable of maintaining seedling establishment under saline conditions is crucial for sustainable melon production. This study evaluated tolerance and antioxidant responses in different melon cultivars using seed treatments to mitigate salt stress effects. The experiment was conducted in two stages under a completely randomized design with four replicates of 50 seeds. In the first stage, a 3 × 5 factorial design tested three salinity levels (0, 60, and 120 mM NaCl) and five cultivars (‘Dali’, ‘Premier’, ‘Supreme’, ‘Imperial 45’, and ‘Asturia’), assessing morphological, physiological, and biochemical traits. In the second stage, two contrasting cultivars (‘Imperial 45’ (sensitive) and ‘Asturia’ (tolerant)) were exposed to salinity combined with stress attenuators, including hydropriming, gibberellic acid, ascorbic acid, salicylic acid, and hydrogen peroxide. Additional biochemical markers and antioxidant enzyme activities were analyzed. Results showed that gibberellic acid and ascorbic acid enhanced antioxidant activity and reduced oxidative damage, particularly in Imperial 45, whereas hydrogen peroxide was more effective in Asturia. Based on their physiological and biochemical responses during germination and early seedling development, Asturia and Imperial 45 were identified as tolerant and sensitive to salt stress, respectively. These findings indicate that the effectiveness of seed treatments depends on cultivar-specific physiological characteristics at the seedling stage. Full article

Understanding how consumers perceive the value of travel packages is essential for pricing and product design. Grounded in behavioral economics frameworks, such as Prospect Theory and Mental Accounting, this study analyses satisfaction across three progressive travel packages before and after explicit price disclosure, exploring multi-attribute service valuation and the moderating influence of traveller profiles. Using a quantitative approach with 387 higher education participants, expected satisfaction was measured through a two-phase price disclosure design. Inferential statistical analyses revealed that satisfaction levels decreased significantly for all packages once prices were revealed, with the sharpest decline occurring in the highly comprehensive, all-inclusive option, validating a psychological threshold of value saturation. Packages comprising only essential elements (flights, accommodation with breakfast and insurance) yielded the highest consistent post-price satisfaction, with these core structural components identified as the absolute most valued attributes. Findings suggest that explicit price disclosure acts as a negative moderator of expected satisfaction, triggering an immediate psychological pain of paying, particularly among independent travellers who exhibit higher price sensitivity and remain more analytical of bundled configurations than users of physical travel agencies. This study provides a framework for stakeholders to avoid over-bundling and optimize product efficiency. Furthermore, it contributes to academic discourse on generational consumer behaviour by highlighting how individual travel organization profiles within an emerging European cohort shape the perceived utility and fairness of tourism pricing. Full article

Soil surface moisture (SSM) is a critical indicator of agricultural drought, yet high-resolution projections under climate change remain scarce. This study develops a machine learning framework to predict and project SSM at 1 km resolution across five European Living Labs (LLs), encompassing vineyards, olive groves, and fruit tree systems. Historical Sentinel-1 SSM observations (2014–2024) were used to train ensemble models (Random Forest, XGBoost, ExtraTrees, LightGBM) incorporating climate variables, soil texture, topography, and land use. Tree-based models achieved R² values of 0.63–0.87. Vineyards showed the highest predictability (R² ≈ 0.87), reflecting their sensitivity to short-term atmospheric demand and surface water availability, whereas olive groves were the least predictable (R² ≈ 0.63–0.68), consistent with deeper rooting systems and greater drought buffering capacity. When forced with bias-corrected CMIP6 projections under SSP1-2.6 and SSP5-8.5 for 2041–2070, models indicate minimal changes under SSP1-2.6 but pronounced SSM declines of 8–24% under SSP5-8.5, with historically wetter regions experiencing the largest absolute losses. SHAP analysis confirmed precipitation and potential evapotranspiration as dominant predictors across all crops. This framework provides spatially explicit, crop-relevant SSM projections to support climate adaptation in European agricultural landscapes. Full article

Fouling limits the performance and lifetime of polyethersulfone (PES) ultrafiltration membranes. We investigated the effect of blending polyaniline (PAni·DBSA) into PES on membrane morphology, wettability, permeability and antifouling behavior, and we evaluated a simple electrochemical cleaning protocol for fouled membranes. A series of PES/PAni·DBSA membranes with different PAni loadings were characterized by SEM, BET, AFM, contact angle, TGA and porosity analysis. Initial water flux (J), bovine serum albumin (BSA) rejection (RR) and flux recovery ratio (FRR) were measured in a dead-end filtration cell. Electrochemical cleaning was applied to selected fouled membranes, and post-cleaning flux and rejection were measured. PAni·DBSA incorporation produced a hierarchical pore structure and altered near-surface texture. Contact angle decreased from 76° to 54°, and swelling increased for intermediate PAni loadings. Initial pure-water fluxes ranged from 5.9 to 39.3 L·m⁻²·h⁻¹. When expressed as absolute percentages, the best performing membrane in terms of reversible fouling recovered 8.12 times of its initial flux. Multivariate analysis indicates that surface hydration and height distribution explain more variance in FRR than Rq alone, consistent with a synergistic role of texture and wettability. Electrochemical treatment substantially increased both flux and rejection for tested membranes, indicating effective foulant mobilization. Full article

This study analyzes the educational and scientific potential of paleogeographic reconstruction as a contextualized geoscience teaching tool within the UNESCO Global Geopark of M’Goun (Central High Atlas, Morocco). It addresses a major limitation of Moroccan geology curricula, which mainly rely on generalized paleogeographic models disconnected from local geological realities and field evidence. The Ouaouizaght sector, characterized by a continuous Jurassic–Cretaceous sedimentary succession and well-preserved Middle Liassic facies, was selected as a representative case study for developing an integrated field-based educational framework. The methodological approach combines cartographic analysis, geological field observations, structural interpretation, and GIS-based spatial synthesis. Field investigations conducted along a northwest–southeast transect enabled the characterization of carbonate platform, slope, and distal hemipelagic environments. Meanwhile, they identified tectonic controls influencing facies organization and basin geometry. The integration of lithostratigraphic, paleoenvironmental, and structural data led to the reconstruction of a coherent paleogeographic model for the western edge of the Central High Atlas during the Middle Liassic. The main target audience of this research is Life and Earth Sciences (LES) teachers, both in initial training and continuing professional development, and indirectly secondary school students. This study highlighted the pedagogical value of combining fieldwork, spatial reasoning, and geological interpretation to support inquiry-based and contextualized geoscience education. Full article

Biodegradable films based on polysaccharides have attracted attention as sustainable alternatives for food preservation. In this study, films and films were developed using cassava starch, chitosan, and grape seed extract, either individually or in polymeric blends, and their physicochemical, mechanical, microstructural, and preservative properties were evaluated. The films were applied to peeled shrimp stored under refrigeration for six days. Microbiological analysis showed a reduction in aerobic mesophilic bacterial counts in coated samples, indicating improved preservation. Films containing cassava starch and chitosan provided greater pH stability during storage. Film characterization revealed that grape seed extract influenced thickness and solubility, particularly in chitosan-based formulations. Cassava starch films exhibited the best water vapor permeability, while blended systems demonstrated improved mechanical performance. The highest tensile strength was observed for the chitosan-based film with extract, whereas starch-containing blends showed balanced strength and flexibility. Scanning electron microscopy revealed more cohesive and continuous structures in polymer blends, while extract-only films presented internal voids, explaining their lower mechanical resistance. Thus, the synergistic combination of cassava starch and chitosan, modulated by grape seed extract, produced films with suitable barrier, mechanical, and structural properties. These biodegradable polymeric films show promising potential for extending the shelf life of refrigerated shrimp and for application in sustainable food packaging. Full article

Programmed Cycle Pruning (PCP) in Arabica coffee can positively influence plant physiology by modifying plant architecture, promoting a more uniform distribution of branches and leaves, and altering microclimatic conditions within the canopy, particularly light incidence. These structural changes may contribute to improvements in plant performance and productivity. The objective of this study was to evaluate growth, yield, and physiological responses of Arabica coffee plants managed under PCP at different stem densities per hectare. The experiment was conducted in a randomized block design with four replications. Treatments were arranged in a 4 × 2 factorial scheme with an additional treatment representing the traditional pruning system. The factorial combination included four stem densities (4000, 8000, 12,000, and 16,000 stems ha⁻¹) and two data collection positions on the plant (lower and upper canopy strata). The evaluated variables included canopy diameter, plagiotropic branch length, number of inflorescences per branch, net photosynthetic rate (Anet), stomatal conductance (gs), leaf transpiration (E), vapor pressure deficit between leaf and air (VPDleaf/air), SPAD index, anthocyanin and flavonoid contents, and grain yield. PCP promoted greater uniformity in leaf gas exchange within the canopy and prevented the occurrence of “girdling”, which under traditional pruning reduced Anet in the upper canopy. Net photosynthesis increased with stem density under PCP. Although growth variables were similar between pruning systems, yield was higher under PCP, with a nonlinear response to stem density, indicating improved canopy gas-exchange uniformity and productivity in Arabica coffee cultivation. Full article