Search Results (15)

Leptadenia pyrotechnica Forssk. Decne (LP) is a medicinal herb from the Asclepiadaceae family with many advantageous properties. The goal of this research is to identify, quantify, and evaluate the antioxidant potential of LP to validate its remarkable therapeutic advantages. The hot soxhlet extraction method was employed to prepare different extracts of LP (stem and root). These extracts were evaluated physiochemically to check their impurity, purity, and quality; qualitatively to detect different phytochemicals; and quantitatively for phenol, saponin, tannin, flavonoid, and alkaloid contents. Then, the in vitro antioxidant potential was estimated by DPPH, NO, H₂O₂ scavenging assays, and MC and FRAP assays. The most prevalent phytochemicals of LP were then analysed by AAS, FT-IR, UV–visible, and GC-MS techniques. A higher extractive yield was shown by LPSE and LPRE (7.37 ± 0.11 and 5.70 ± 0.02). The LP stem showed better physicochemical and qualitative results than the root. The quantitative and in vitro antioxidant results indicated maximal phenols, tannins, and alkaloid contents in LPSE, which was further confirmed by UV–visible, FT-IR, and GC-MS results. The extraction methods (soxhlation or ultrasonication) were optimized by utilizing RSM to determine the impacts of multiple parameters. The study concluded that the plant has remarkable therapeutic advantages to promote additional clinical investigations and the mechanisms of its action. Full article

To the best of our knowledge, there was no prior report providing valuable preliminary data through a demonstration of the quantitative phytochemical and antioxidant activity of Gymnosporia senegalensis. The total contents of phenols, flavonoid, flavanol, tannin, and saponin were evaluated from different fractions extracted from the leaf, stem, and bark of G. senegalensis by using standards such as gallic acid, quercetin, rutin, tannic acid, and saponin quillaja. The antioxidant potential was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide scavenging (H₂O₂), superoxide anion radical scavenging, metal chelating ferrous ion, ferric reducing antioxidant power (FRAP), and total antioxidant capacity (TAC). Data were subjected to half-inhibitory concentration (IC₅₀) and one-way analysis of variance (ANOVA) at p < 0.05 as a significant value. The total phenol content was found to be highest in the chloroform extract of stem at 97.7 ± 0.02 mg GAE/g. The total flavonoid and flavonol contents in the aqueous extract were 97.1 ± 0.03 mg QE/g and 96.7 ± 0.07 mg RE/g, respectively. The total tannin content in the ethyl acetate extract of leaf was 97.5 ± 0.01 mg TAE/g, and the total saponin content in the methanol extract of stem was 79.1 ± 0.06 mg SQE/g. The antioxidant analysis indicated that IC₅₀ and percentage (%) inhibition were dose-dependent and showed the highest antioxidant activity (40.9 ± 0.9 µg/mL) in methanol extract of leaf for DPPH, (88.8 ± 1.12 µg/mL) in the chloroform extract of stem for H₂O₂, (43.9 ± 0.15 µg/mL) in the aqueous extract of bark for superoxide anion radical scavenging activity, (26.9 ± 0.11 µg/mL) in the chloroform extract of leaf for the metal chelating ferrous ion activity, (7.55 ± 0.10 mg/mL) in the benzene extract of leaf for FRAP, and (2.97 ± 0.01 mg/mL) in the methanol extract of bark for TAC. These results show that G. senegalensis has great potential in antioxidant activities. The isolation and characterization of specific bioactive compounds and the in vivo applicability of such activity await further extensive studies for drug discovery and development. Full article

In this study, the impacts of long-term soil and crop management practices on crop productivity and soil health in oilseed brassica-based production systems were examined. Different tillage, crop residue recycling and cropping systems (fallow–mustard, cluster bean–mustard, green gram–mustard, maize–mustard, pearl millet–mustard and sesame–mustard) were studied for 5 years at two soil depths (0–15 and 15–30 cm) in a split-plot design with three replications. No-till permanent beds with crop residue (PB + R) noticeably improved soil organic carbon (SOC), microbial biomass carbon (MBC), enzymes (dehydrogenase (DHA) and alkaline phosphatase (AlP)), nitrogen fractions (available and total nitrate) and available phosphorus and potassium content in both soil layers compared to conventional tillage without crop residues. However, the plough soil layer (0–15 cm) showed higher concentrations of soil carbon, enzymes, N fractions and available P than in the subsoil (15–30 cm). The dynamic soil biological and chemical properties also varied with the crop stage, and higher MBC at 30 days, SOC and enzymatic activities at 60 days, and N fractions and available P and K during the harvesting of mustard crop were recorded. Green gram–mustard rotation showed higher values in terms of biological and chemical parameters. Thus, the legume-based mustard crop rotation following no-till permanent beds and residue recycling was found to be holistic in terms of improving soil health and nutrient cycling. Full article

Plant roots aid the growth and functions of several kinds of microorganisms such as plant growth-promoting rhizobacteria, mycorrhizal fungi, endophytic bacteria, actinomycetes, nematodes, protozoans which may impart significant impacts on plant health and growth. Plant soil–microbe interaction is an intricate, continuous, and dynamic process that occurs in a distinct zone known as the rhizosphere. Plants interact with these soil microbes in a variety of ways, including competitive, exploitative, neutral, commensal, and symbiotic relationships. Both plant and soil types were found to have an impact on the community diversity and structure of the rhizosphere, or vice versa. The diversity of microorganisms in soil is thought to be essential for the management of soil health and quality because it has different plant growth-promoting or biocontrol effects that could be very advantageous for the host plant and alter plant physiology and nutrition. The composition of microbial community is influenced by soil and plant type. Besides these beneficial microbes, the soil also harbors microorganisms that are detrimental to plants, competing for nutrients and space, and causing diseases. Numerous microorganisms have antagonistic activity and the ability to defend plants from soil-borne diseases. The study of the soil microbiome is essential for formulating strategies for transforming the rhizosphere to the benefit of the plants. This review pays special emphasis on the types of microbial populations in the soil and how they influence plant growth, nutrient acquisition, inter-relationships between soil microbes and plants, stress resistance, carbon sequestration, and phytoremediation. Full article

Cicer arietinum L. (Bengal gram, chickpea) is one of the major pulse crops and an important part of traditional diets in Asia, Africa, and South America. The present study was conducted to determine the changes in total isoflavones during sprouting (0, 3, and 7 days) along with the effect of two precursor supplementations, p-coumaric acid (p-CA) and L-phenylalanine (Phe), in C. arietinum. It was observed that increasing sprouting time up to the seventh day resulted in ≈1282 mg 100 g⁻¹ isoflavones, which is approximately eight times higher than chickpea seeds. The supplementation of Phe did not affect the total length of sprouts, whereas the supplementation of p-CA resulted in stunted sprouts. On the third day of supplementation with p-CA (250 mg L⁻¹), the increase in the total phenolic content (TPC) (80%), daidzein (152%), and genistin (158%) contents were observed, and further extending the supplementation reduced the growth of sprouts. On the seventh day of supplementation with Phe (500 mg L⁻¹), the increase in TPC by 43% and genistin content by 74% was observed compared with non-treated sprouts; however, the total isoflavones content was found to be 1212 mg 100 g⁻¹. The increased TPC was positively correlated with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (r = 0.787) and ferric-reducing antioxidant potential (FRAP) (r = 0.676) activity. This study suggests that chickpea sprouts enriched in TPC and antioxidants can be produced by the appropriate quantity of precursor supplementation on a particular day. The results indicated major changes in the phytochemical content, especially daidzein and genistin. It was also concluded that the consumption of 100 g of seventh-day sprouts provided eight times higher amounts of isoflavones in comparison to chickpea seeds. Full article

Dates are considered high energy nutritional fruits as they are packed with plenty of minerals, vitamins and sugars. Among various options available for processing and value addition, dry dates are one of the best possible alternatives to convert doka/khalal stage fruit into a high value product. However, the technology for their production is limited to a few traditional date growing countries and research is limited in the countries with expanding areas. Thus, the protocol for development of dry dates was standardised and the effect of different blanching intervals (5, 10 and 15 min) on the drying and quality parameters were studied. The rate of drying, dehydration ratio and rehydration ratio were found to increase with increasing hot water dipping durations while a 10 min hot water dip was found to be best for retaining the best sensory parameters of taste, colour/appearance and flavour. The total colour difference (ΔE) was also maximal (71.10) in the date fruits exposed to a 10 min dip treatment. The reducing sugars decreased and non-reducing sugars increased with increasing hot water dipping periods while there was no significant variation in the total sugar content of the treated dates. The tannins responsible for acrid taste were also reduced to a minimum (1.43%) compared to control (2.52%) fruits. Thus, the fruits of date cv. Medjool being bulky and fibrous with more dry matter content are well suited for dry date preparation. Full article

The present review paper is an attempt to examine and provide an overview of the various conservation strategies and regulatory framework to protect endangered plants, including Chlorophytum tuberosum, popularly known as Safed Musli in the local language. C. tuberosum belongs to the family Liliaceae and is being used in the indigenous systems of medicine as a galactagogue, aphrodisiac, antitumor, immunomodulatory, antidiabetic, analgesic, anti-inflammatory, hypolipidemic, anti-ageing, antimicrobial, etc. This plant has great medicinal and commercial value and is part of the Biological Diversity Act, but due to a lack of effective conservation, it is on the verge of extinction because of natural and manmade reasons, such as loss of habitat, climate change, pollution, excessive harvesting, etc. The most valuable medicinal plants have great importance; hence, many conservation techniques are being employed to protect them. In furtherance to the conservation of such plant species, strategic efforts, in the form of laws and policies, are laid; however, existing legislative mechanisms and policy parameters are not sufficient to overcome the challenges of conservation of such plant species, including Safed Musli, hence, this plant has been considered as a critically endangered plant in India. It is pertinent to note that we do not have specific legislation enacted for the protection of plant species; however, efforts are being made to conserve it under various laws, such as the Forest Conservation Act, Biological Diversity Act 2002, and many other allied legislations. This basic legislation of the Biological Diversity Act also lacks focal attention on the conservation of endangered plant species. Moreover, decentralization of power and actual community participation in conservation practices are also missing. A cumulative effect of both scientific measures and legal mechanisms supported by community participation may produce better results in the conservation of plant species, including Safed Musli. The protection of rich sources and biological diversity is not being taken as seriously as it ought to be, hence, it is necessary to improve awareness and public participation in conservation techniques with effective legislation for the conservation of highly endangered plant species. Full article

Large wind turbines of the horizontal axis are commonly used to gather wind energy; however, their performance is found to be constrained in conditions of erratic and low-speed wind flow. In contrast, low wind conditions—which are typically present in dense urban areas—are found to favour vertical axis wind turbines (VAWT). These turbines have a simple design, are inexpensive and quiet, and are discovered to be better in low wind situations. In this research, we have chosen wind tree applications to absorb the most available wind energy. The new Aeroleaf Savonius Wind Turbine was developed numerically and a computational fluid dynamics simulation was performed on this new type of Savonius tree to predict its performance. The results indicated that the system could accept wind from any direction and could start rotating as soon as the site had a cut in wind speed of 3.3 m/s. The rotor speed increased by 10.4% from 5.5 to 6.3 m/s wind speed at 0.45 tip speed ratio. The tip speed ratio is 0.52 at the site’s high wind speed, and under these circumstances, the maximum Cp is 12.9%. The turbine was able to produce superior performance coefficients, according to the results. Full article

Although India has a well-established and growing economy surrounding synthetic drug chemistry with an antibiotic base, a large part of the population, especially in forested villages and tribal belts, is relying solely on plant-derived drugs. This is due to a lower number of side effects, low chances of resistance development against pathogenic microorganisms, as well as the diversity and affordability of such drugs. In the Indian subcontinents, Euphorbia neriifolia Linn. (EN) is one of the valuable plants from the big family of Euphorbiaceae, which is usually found in rocky and hilly areas. E. neriifolia was found to be useful in curing tumors, abdominal swelling, bronchial infection, hydrophobia, earache, cough and cold, asthma, leprosy, gonorrhea, spleen enlargement, leucoderma, snake bites, scorpion stings, and causing appetite improvement, etc. Different in vitro and in vivo experimental studies were performed to determine the antioxidant, anti-diabetic, immunomodulatory, anti-inflammatory, anti-arthritic, wound healing, anti-atherosclerosis, radioprotective, anti-anxiety, anti-convulsant, anti-psychotic, anti-thrombotic, dermal irritation, hemolytic, analgesic, anti-fertility, diuretic, anti-microbial, anti-diarrheal, and anti-carcinogenic activities of the various parts of EN. Several bioactive compounds, such as euphol, nerifoliol, taraxerol, euphonerins A–G, lectin, etc., were isolated from E. neriifolia and need to be investigated further for various biological activities (cardiovascular and neuronal diseases). In the pharmaceutical sector, E. neriifolia was selected for the development of new drugs due to its broad pharmacological activities. Therefore, in the present review, distribution, classification, morphological and microscopical description, phytochemical investigation, pharmacological activities, medicinal uses, harmful effects, and their treatment were evaluated, especially against different lifestyle-related diseases. Full article

Plants are constantly exposed to both biotic and abiotic stresses which limit their growth and development and reduce productivity. In order to tolerate them, plants initiate a multitude of stress-specific responses which modulate different physiological, molecular and cellular mechanisms. However, many times the natural methods employed by plants for overcoming the stresses are not sufficient and require external assistance from the rhizosphere. The microbial community in the rhizosphere (known as the rhizomicrobiome) undergoes intraspecific as well as interspecific interaction and signaling. The rhizomicrobiome, as biostimulants, play a pivotal role in stimulating the growth of plants and providing resilience against abiotic stress. Such rhizobacteria which promote the development of plants and increase their yield and immunity are known as PGPR (plant growth promoting rhizobacteria). On the basis of contact, they are classified into two categories, extracellular (in soil around root, root surface and cellular space) and intracellular (nitrogen-fixing bacteria). They show their effects on plant growth directly (i.e., in absence of pathogens) or indirectly. Generally, they make their niche in concentrated form around roots, as the latter exude several nutrients, such as amino acids, lipids, proteins, etc. Rhizobacteria build a special symbiotic relationship with the plant or a section of the plant’s inner tissues. There are free-living PGPRs with the potential to work as biofertilizers. Additionally, studies show that PGPRs can ameliorate the effect of abiotic stresses and help in enhanced growth and development of plants producing therapeutically important compounds. This review focuses on the various mechanisms which are employed by PGPRs to mitigate the effect of different stresses in medicinal plants and enhance tolerance against these stress conditions. Full article

Environmental stress is a major challenge for sustainable food production as it reduces yield by generating reactive oxygen species (ROS) which pose a threat to cell organelles and biomolecules such as proteins, DNA, enzymes, and others, leading to apoptosis. Plant growth-promoting rhizobacteria (PGPR) offers an eco-friendly and green alternative to synthetic agrochemicals and conventional agricultural practices in accomplishing sustainable agriculture by boosting growth and stress tolerance in plants. PGPR inhabit the rhizosphere of soil and exhibit positive interaction with plant roots. These organisms render multifaceted benefits to plants by several mechanisms such as the release of phytohormones, nitrogen fixation, solubilization of mineral phosphates, siderophore production for iron sequestration, protection against various pathogens, and stress. PGPR has the potential to curb the adverse effects of various stresses such as salinity, drought, heavy metals, floods, and other stresses on plants by inducing the production of antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase. Genetically engineered PGPR strains play significant roles to alleviate the abiotic stress to improve crop productivity. Thus, the present review will focus on the impact of PGPR on stress resistance, plant growth promotion, and induction of antioxidant systems in plants. Full article

The stationary life of plants has led to the evolution of a complex gridded antioxidant defence system constituting numerous enzymatic components, playing a crucial role in overcoming various stress conditions. Mainly, these plant enzymes are superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione S-transferases (GST), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR), which work as part of the antioxidant defence system. These enzymes together form a complex set of mechanisms to minimise, buffer, and scavenge the reactive oxygen species (ROS) efficiently. The present review is aimed at articulating the current understanding of each of these enzymatic components, with special attention on the role of each enzyme in response to the various environmental, especially abiotic stresses, their molecular characterisation, and reaction mechanisms. The role of the enzymatic defence system for plant health and development, their significance, and cross-talk mechanisms are discussed in detail. Additionally, the application of antioxidant enzymes in developing stress-tolerant transgenic plants are also discussed. Full article

Fire is one of the mutable hazards that damage properties and destroy forests. Many researchers are involved in early warning systems, which considerably minimize the consequences of fire damage. However, many existing image-based fire detection systems can perform well in a particular field. A general framework is proposed in this paper which works on realistic conditions. This approach filters out image blocks based on thresholds of different temporal and spatial features, starting with dividing the image into blocks and extraction of flames blocks from image foreground and background, and candidates blocks are analyzed to identify local features of color, source immobility, and flame flickering. Each local feature filter resolves different false-positive fire cases. Filtered blocks are further analyzed by global analysis to extract flame texture and flame reflection in surrounding blocks. Sequences of successful detections are buffered by a decision alarm system to reduce errors due to external camera influences. Research algorithms have low computation time. Through a sequence of experiments, the result is consistent with the empirical evidence and shows that the detection rate of the proposed system exceeds previous studies and reduces false alarm rates under various environments. Full article

At present, traditional visual-based surveillance systems are becoming impractical, inefficient, and time-consuming. Automation-based surveillance systems appeared to overcome these limitations. However, the automatic systems have some challenges such as occlusion and retaining images smoothly and continuously. This research proposes a weighted resampling particle filter approach for human tracking to handle these challenges. The primary functions of the proposed system are human detection, human monitoring, and camera control. We used the codebook matching algorithm to define the human region as a target and track it, and we used the practical filter algorithm to follow and extract the target information. Consequently, the obtained information was used to configure the camera control. The experiments were tested in various environments to prove the stability and performance of the proposed system based on the active camera. Full article

This paper proposes a novel approach for selecting a subset of features in semi-supervised datasets where only some of the patterns are labeled. The whole process is completed in two phases. In the first phase, i.e., Phase-I, the whole dataset is divided into two parts: The first part, which contains labeled patterns, and the second part, which contains unlabeled patterns. In the first part, a small number of features are identified using well-known maximum relevance (from first part) and minimum redundancy (whole dataset) based feature selection approaches using the correlation coefficient. The subset of features from the identified set of features, which produces a high classification accuracy using any supervised classifier from labeled patterns, is selected for later processing. In the second phase, i.e., Phase-II, the patterns belonging to the first and second part are clustered separately into the available number of classes of the dataset. In the clusters of the first part, take the majority of patterns belonging to a cluster as the class for that cluster, which is given already. Form the pairs of cluster centroids made in the first and second part. The centroid of the second part nearest to a centroid of the first part will be paired. As the class of the first centroid is known, the same class can be assigned to the centroid of the cluster of the second part, which is unknown. The actual class of the patterns if known for the second part of the dataset can be used to test the classification accuracy of patterns in the second part. The proposed two-phase approach performs well in terms of classification accuracy and number of features selected on the given benchmarked datasets. Full article