Search Results (120)

Background/Objectives: Viral respiratory infections have a significant impact on global health and the economy. While vaccines are effective in preventing infection, they might not be available or sufficient when used alone and must be complemented by specific therapeutic strategies. The development of new antiviral agents is increasingly important due to the continual emergence of novel respiratory pathogens. Previously we identified bovine lactoferrin (bLf)-derived tetrapeptides and peptidomimetics that showed potent in vitro activity against the influenza A virus in the picomolar range. Methods: Inspired by these results, in this study, we evaluated the antiviral potential of these compounds against HCoV-229E, a human coronavirus that can cause severe disease in immunocompromised individuals, using a compound repositioning approach. Results: Functional studies revealed that SK(N-Me)HS (3) interferes with viral entry and replication, while compound SNKHS (5) primarily blocks infection in the early stages. Biophysical analyses confirmed the occurrence of high-affinity binding to the viral spike protein, and computational studies suggested that the compounds target a region involved in conformational changes necessary for membrane fusion. Conclusions: These findings highlight these compounds as promising candidates for coronavirus entry inhibition and underscore the value of compound repurposing in antiviral development. Full article

Honey bees are vital to ecosystem conservation, agricultural production, and biodiversity, yet their welfare has often been overlooked. This study introduces the integration of Honey Bee Welfare Practices (HBWPs) into the One Welfare framework, addressing the interconnectedness of honey bee welfare, environmental welfare, and human wellbeing. We analysed and re-evaluated the 243 HBWPs already identified and categorised within the context of the Five Domains model in 2024 by Giovanni Formato et al., and we explored their broader impacts. By incorporating the One Welfare approach, we assessed each practice’s effect on bee welfare both as individuals and as a superorganism, human wellbeing, and environmental welfare, as well as their economic and time-related implications for beekeepers. The aim of this study was to obtain a list of One Welfare Practices in Beekeeping, considering all stakeholders as equally important. The analysis highlights the multidimensional nature of beekeeping, with 280 practices positively affecting honey bee welfare, while also considering their potential impact on human wellbeing, environmental health, and production. Challenges such as balancing beekeeper time constraints and welfare goals are discussed, with recommendations for practical compromises. This approach can offer a holistic and sustainable model for apiculture, ensuring that welfare is maintained across all stakeholders, and provides a flexible framework applicable to various beekeeping systems worldwide. Full article

Bisphenol A (BPA) is an endocrine-disrupting chemical with estrogen-like activity, known to impair immune function. BPA may act as a pro-inflammatory agent, reducing immune response efficacy, increasing bacterial load in E. coli infections, and altering immune responses in parasitic infections (Leishmania major, Nippostrongylus brasiliensis, Toxocara canis) through cytokine and regulatory T-cell modulation. Following its ban in food contact materials in Europe, several analogs have been introduced. This study assessed the immunotoxicity of BPA and six analogs, namely BPAP, BPE, BPP, BPS-MAE, BPZ, and TCBPA, by evaluating in vitro the antibody production. Peripheral blood mononuclear cells from healthy male and female donors were exposed to increasing concentrations of each compound for 24 h. After stimulation with rhIL-2 and ODN2006, IgM and IgG secretion were measured on day six. All compounds suppressed antibody production in a concentration-dependent manner, with some sex-related differences. IC₅₀ values showed BPP as the most potent suppressor, and BPE as the weakest. Similarly, IC₂₀ values confirmed these differences in potency, except for BPA being the weakest for IgM in males. Overall, te results do not support the idea that BPA analogs are safer than BPA. Full article

The right heart–pulmonary circulation unit (RH-PCU) constitutes an integrated anatomo-functional system characterized by high-volume blood flow, low intravascular pressure, and minimal pulmonary vascular resistance. The RH-PCU dysfunction is a challenge for clinicians, as it can result from numerous pathological conditions, each with different clinical presentations. The pathophysiological changes underlying the hemodynamic alterations in the pressure and volume affecting the right ventricle can lead the patient to present with the primary symptom: dyspnea. We review the clinical presentation, the laboratory test, and the role of multimodality imaging in the evaluation of the disfunction of the RHPCU, including echocardiography, stress echocardiography, computed tomography, magnetic resonance imaging, nuclear imaging, and invasive pressure measurement through catheterization. We therefore aimed to describe the various diagnostic options available to clinicians, evaluating their effectiveness and limitations of use. Full article

The administration of nutraceutical substances to fish diet can help to control disease outbreaks in aquaculture practices, thereby promoting sustainability and food safety. In particular, some substances have the potential to alleviate the effects of trace metals toxicity in fish also by reducing metal accumulation in tissues. This study evaluates, for the first time, the effect of nutraceutical substances on bioaccumulation mechanisms of mercury (Hg) in tissues and organs of farmed gilthead seabream (Sparus aurata) by mesocosm experimentation. The kinetics of bioaccumulation in muscle, gills, gut, liver and kidney and the detoxification efficiency were also assessed. Fish were fed with three different diets: a commercial diet used as control (CD); a diet enriched with short chain fatty acids (SCFA) and extract of Castanea sativa (D1); a diet enriched with yeast Saccharomyces cerevisiae and extract of Schinopsis balansae (D2). All groups were exposed to sub-lethal concentrations of mercury. After 20 days of exposure, mercury levels in different organs and tissues clearly revealed the effectiveness of yeast and plant extracts in limiting the metal bioaccumulation in fish fed with D2 through mercury absorption and then elimination by feces. In contrast, the D1 seems to not reduce the Hg bioaccumulation in fish tissues. This can be attributed to the high affinity of SCFA for mercury, leading to the formation of organometallic compounds absorbed by the fish tissues. This mechanism potentially counteracts the efficiency of tannins contained in the extract plant on mercury removal. This study clearly demonstrates that the use of diets enriched with yeast and/or plant extracts rich in tannins are a useful bioremediation strategy to reduce trace metals bioaccumulation in farmed fish, thus preserving their health status from intoxication, their commercial values, and consequently the health of consumers. Full article

The challenges associated with climate change have led to the need for pro-environmental behaviors, including the consumption of sustainable novel foods. Despite the importance of sustainable food for the environment, there is still a need to further investigate the psychological determinants of consumer behavior change putatively able to promote the use of novel foods. In line with this, the aim of the present study was to investigate the role of readiness to change (RTC) in shaping the intention to consume sustainable foods (e.g., chia seeds and edible insects). RTC refers to a valuable construct composed of seven different dimensions, namely perceived importance of the problem/change, motivation, self-efficacy, effectiveness of proposed solution, social support, action and involvement, and perceived readiness. In keeping with this, a cross-sectional study was conducted by collecting from 1252 participants through an online and anonymous survey. In line with the aim above, a linear discriminant analysis was performed to explore potential non-linear relationships between RTC and novel food consumption. The results highlighted certain RTC dimensions (e.g., perceived importance of the problem, action, and self-efficacy) able to positively support the intention to consume novel foods (e.g., chia seeds and spirulina algae). In conclusion, the study pointed out evidence regarding psychological determinants in terms of RTC able to improve sustainable behaviors, namely the use of novel foods. In the context of sustainability, the present study represents a groundwork for the implementation of future studies in this field of research as well as the development of future policies aimed at promoting awareness and encouraging the adoption of sustainable eating behaviors. Full article

Vitamin D plays a vital role in human physiology, including a crucial role in regulating bone metabolism and various extra-skeletal effects. Calcitriol exerts anti-inflammatory effects on monocytes and macrophages by increasing IL-10 production and decreasing the production of proinflammatory IL-1β, IL-6, tumor necrosis factor-α (TNF-α), receptor activator of nuclear factor kappa-Β ligand (RANKL), and cyclo-oxygenase-2 (COX-2). In addition, calcitriol also exerts important effects on adaptive immunity by downregulating MHC-II class and co-stimulatory molecules on antigen-presenting cells, but it also directly affects T lymphocytes. In multiple studies, the influence of vitamin D on eye diseases, including corneal diseases, has been demonstrated. Adequate vitamin D supplementation in patients with dry eye significantly improves tear quality and consequently heals the epithelial cells of the ocular surface. Pterygium is a fibrovascular growth of conjunctival tissue that histologically consists of superficial conjunctival epithelium and an underlying fibrovascular layer. The prevalence of pterygium depends on the region. In zones closer to the equator—“pterygium zone”, it is up to 22%, and outside of them it can be even less than 2%. While UV radiation is recognized as a primary risk factor, other factors, including vitamin D, may influence its development. This review aims to summarize the effects of vitamin D on the pathophysiological mechanism of pterygium and its possible therapeutic impact. Current research suggests that vitamin D is protective through its immunomodulatory and anti-inflammatory properties. Finally, there is still insufficient evidence of the therapeutic benefit of vitamin D in pterygium, and future large-scale randomized controlled studies are needed to elucidate the exact role of vitamin D in pterygium onset and recurrence as well as its potential therapeutic benefit. Full article

Rett syndrome (RTT) is a rare X-linked dominant neurodevelopmental disorder caused by pathogenic variants in the methyl-CpG-binding protein 2 (MECP2) gene, which encodes a methyl-CpG-binding protein (MeCP2) that acts as a repressor of gene expression, crucial in neurons. Dysfunction of MeCP2 due to its pathogenic variants explains the clinical features of RTT. Here, we performed histological and RNA analyses on a post-mortem brain sample from an RTT patient carrying the p.Arg106Trp missense mutation. This patient is part of a cohort of 56 genetically and clinically characterized RTT patients, for whom we provide an overview of the mutation landscape. In the RTT brain specimen, RT-PCR analysis detected preferential transcription of the mutated mRNA. X-inactivation studies revealed a skewed X-chromosome inactivation ratio (95:5), supporting the transcriptional findings. We also mapped the MECP2 transcript in control human brain regions (temporal cortex and cerebellum) using the RNAscope assay, confirming its high expression. This study reports the MECP2 transcript representation in a post-mortem RTT brain and, for the first time, the in situ MECP2 transcript localization in a human control brain, offering insights into how specific MECP2 mutations may differentially impact neuronal functions. We suggest these findings are crucial for developing RNA-based therapies for Rett syndrome. Full article

Machine learning-based tools are becoming increasingly popular in clinical practice. They offer new possibilities but are also limited in their reliability and accuracy. The present systematic review updates and discusses the existing literature regarding machine learning algorithm-based identification of cruciate ligament injury on radiographic images. PubMed was searched for articles containing machine learning algorithms related to cruciate ligament injury recognition. No additional filters or time constraints were used. All eligible studies were accessed by hand. From the 115 articles initially retrieved, 29 articles were finally included. Only one study included the posterior cruciate ligament (PCL). Deep learning algorithms in the form of convolutional neural networks (CNNs) were most frequently used. Many studies presented CNNs that identified binary decision classes of regular and torn anterior cruciate ligaments (ACLs) with a best sensitivity of 0.98, a specificity of 0.99, and an AUC ROC of 1.0. Other studies expanded the decision classes to partially torn ACLs or reconstructed ACLs, usually at the cost of sensitivity and specificity. Deep learning algorithms are excellent for identifying ACL injuries, tears, or postoperative status after reconstruction on MRI images. They are much faster but only sometimes better than the human reviewer. While the technology seems ready, barriers to ethical and legal issues and clinicians’ refusals must be overcome to some extent. It can be firmly assumed that artificial intelligence will have a future contribution in the diagnosis of cruciate ligament injuries. Full article

Seven hydrophobic deep eutectic solvents (hDESs) were characterised to evaluate their potential applicability in different industries and their environmental impact. Standard physicochemical properties were determined, yielding polarity and density values that were slightly higher for thymol-based hDESs than menthol-based ones, whereas for viscosity, the trend was opposite. Regarding biologically relevant activities, the antioxidative capacity and antimicrobial activity of hDESs were determined. Thymol-based hDESs are more potent as potential antioxidants, especially the one with coumarin as a hydrogen bond acceptor, which had the highest Oxygen Radical Absorbance Capacity (ORAC) value. Antimicrobial activity was assessed on four bacterial strains and one yeast strain. Calculated minimal inhibitory concentrations (MICs) showed that all hDESs possess this activity, and even the antimycotic effect against C. albicans was observed. Furthermore, to ensure the safety of these solvents for human use, in vitro cytocompatibility was determined. hDESs were tested on three human cell lines (HaCaT, CaCo-2, and HeLa), with no cytotoxic effect observed up to 1000 mg L⁻¹. Finally, the environmental impact by the phytotoxicity test and in vitro antioxidative assay on wheat was determined for three selected hDESs, which were found to be slightly toxic, with different effects on plant defence mechanisms against induced antioxidative stress. Overall, the tested terpene-based hDESs demonstrate potential as alternative solvents for various industries, including food production, cosmetics, and pharmaceuticals, with thymol-based variants exhibiting a slight advantage in relation to the parameters evaluated in this study. Full article

Asthma, a chronic airway disease, is marked by allergic inflammation, hyperresponsiveness, and tissue remodeling. Influenza infections in asthma patients can cause severe exacerbations, though the underlying mechanisms remain unclear. This study investigated how pre-existing allergic inflammation affects immune responses to influenza infection in mice exposed to house dust mite (HDM). Mice were repeatedly exposed to HDM, followed by infection with the influenza A virus, and were sacrificed three days post-infection. Plasma was analyzed for HDM-specific immunoglobulins, while lung tissue was used for immune cell flow cytometry and RNA sequencing analysis. HDM exposure induced allergic inflammation, evidenced by more HDM-specific IgE, IgG1, IgG2, eosinophils, neutrophils, Th1, and Th17 cells compared to controls. Upon influenza infection, the effects of HDM and influenza co-infection interacted, showing fewer Th1 cells and regulatory T cells and more Th2 cells compared to mice exposed to the influenza virus alone. Interestingly, RNA-seq analysis revealed less upregulation of Th1-related genes and antiviral pathways in co-exposed mice, suggesting impaired Th1 immunity and antiviral responses. Pre-existing allergic inflammation significantly altered immune responses in mice co-infected with influenza, revealing underdeveloped antiviral responses as early as three days post-infection. These findings may explain the increased susceptibility of patients with asthma to severe viral diseases. Full article

Pterygium is a condition affecting the ocular surface, marked by a triangular-shaped growth of fibrotic tissue extending from the nasal conjunctiva toward the corneal center, potentially causing visual impairment. While ultraviolet (UV )light exposure is the primary risk factor for pterygium, its underlying cause remains unclear. In order to better understand the true genesis of pterygium development, we investigated pterygium tissue and compared it with healthy conjunctiva controls. Given the eye’s direct environmental exposure, we analyzed the microbiota composition using metagenomic sequencing of pterygium tissue to identify microbes potentially associated with this condition. Metagenomic sequencing revealed a higher prevalence of the fungus Malassezia restricta in five pterygium samples, confirmed by in situ hybridization. The CHIT1 gene, which plays a role in antifungal defenses, displayed the highest expression in five pterygium tissue samples compared to healthy conjunctiva controls, suggesting the potential involvement of Malassezia restricta in pterygium development. Gene expression profiling of pterygium highlighted an IL-33 and IL-4 gene expression signature, along with an increased presence of M2 macrophages, emphasizing their role in promoting fibrosis—a hallmark feature of pterygium. The detection of Malassezia restricta in the pterygium samples and associated molecular changes provides novel insights into the ocular microbiome and raises the possibility of Malassezia’s involvement in pterygium pathology. Full article

Background: The Telemechron project addresses critical needs in telemedicine by evaluating technology assessment frameworks and key performance indicators (KPIs), among other objectives. Effective frameworks and KPIs are crucial for assessing telemedicine services, especially in terms of their impact on patient outcomes and service efficiency. Methods: This study adopted a dual approach to assess the contributions of the Telemechron project. Firstly, it applied a technology assessment framework to quantitatively evaluate telemedicine services, focusing on measurable improvements and systematic analysis. Secondly, it investigated a set of predefined KPIs using specific metrics and parameters to evaluate their applicability and limitations in telemedicine settings. Results and Discussion: The technology assessment framework demonstrated significant utility by providing structured, quantifiable improvements across key aspects of telemedicine services. It was effective in evaluating the alignment of services with strategic goals and their integration with existing healthcare systems. The predefined KPIs, although not developed within this study and not directly used by the different operational units (OUs)—which established their own context-specific KPIs—still provided valuable insights into telemedicine performance. However, the study revealed that these KPIs highlighted a need for further customization to enhance their relevance across various contexts. While the KPIs may offer useful performance indicators, their general applicability necessitated adjustments to better address the specific needs of telemedicine applications. The analysis model for the KPI set, in terms of metrics and parameters, is exportable and generalizable to other national and international telemedicine contexts. Conclusions: The study confirms the effectiveness of the framework in delivering measurable improvements in telemedicine services and underscores the importance of adapting KPIs for specific contexts. Future research should focus on further applying and expanding the framework and metrics, refining KPI models, integrating new technologies, and conducting cross-national comparisons to enhance the applicability and effectiveness of telemedicine evaluations. Full article

Broccoli is rich in biologically active compounds, especially polyphenols and glucosinolates, known for their health benefits. Traditional extraction methods have limitations, leading to a shift towards using natural deep eutectic solvents (NADESs) to create high-quality extracts with enhanced biological activity. This study focuses on preparing broccoli extracts in NADES, enriched with polyphenols and glucosinolates, without additional purification steps. Using the COSMOtherm software, the solubility of polyphenols and glucosinolates in NADESs was predicted, and five biocompatible betaine-based NADESs were prepared with glucose (B:Glc_1:1 and B:Glc_5:2), sucrose (B:Suc), glycerol (B:Gly), and malic acid (B:MA) as hydrogen bond donors. The resulting extracts were assessed for total polyphenol and glucosinolate content, along with antioxidant capacity, using the ORAC assay. The results demonstrated that NADES extracts contained higher polyphenol content and exhibited enhanced antioxidant effects compared to the reference ethanol extract, with B:Glc_1:1 extract showing the highest performance among all the extracts tested. On the other hand, the extract based on B:MA exhibited nearly six times higher total glucosinolate content compared to the ethanol extract. Additionally, polyphenols and glucosinolates were generally more stable in NADES extracts than in the reference solvent. Finally, the B:Glc_1:1 extract, identified as optimal in terms of polyphenol and glucosinolate content and stability, exhibited mild stimulation of HaCaT cells growth and facilitated the wound-healing process. Through green chemistry parameter calculations, we demonstrated that the extraction of broccoli bioactives using B:Glc_1:1 can be considered sustainable, underscoring the potential of NADESs for producing ready-to-use plant extracts. Full article

Background: European countries have included healthcare workers (HCWs) among priority groups for COVID-19 vaccination. We established a multi-country hospital network to measure the SARS-CoV-2 incidence and effectiveness of COVID-19 vaccines among HCWs against laboratory-confirmed SARS-CoV-2 infection. Methods: HCWs from 19 hospitals in 10 countries participated in a dynamic prospective cohort study, providing samples for SARS-CoV-2 testing at enrolment and during weekly/fortnightly follow-up. We measured the incidence during pre-Delta (2 May–6 September 2021), Delta (7 September–14 December 2021), and Omicron (15 December 2021–2 May 2023) waves. Using Cox regression, we measured the relative vaccine effectiveness (rVE) of the first COVID-19 booster dose versus primary course alone during Delta and Omicron waves. Results: We included a total of 3015 HCWs. Participants were mostly female (2306; 79%), with a clinical role (2047; 68%), and had a median age of 44 years. The overall incidence of SARS-CoV-2 infection was 3.01/10,000 person-days during pre-Delta, 4.21/10,000 during Delta, and 23.20/10,000 during Omicron waves. rVE was 59% (95% CI: −25; 86) during Delta and 22% (1; 39) during Omicron waves. rVE was 51% (30; 65) 7–90 days after the first booster dose during the Omicron wave. Conclusions: The incidence of SARS-CoV-2 infection among HCWs was higher during the Omicron circulation period. The first COVID-19 vaccine booster provided additional protection against SARS-CoV-2 infection compared to primary course vaccination when recently vaccinated <90 days. This multi-country HCW cohort study addressing infection as the main outcome is crucial for informing public health interventions for HCWs. Full article