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18 pages, 13604 KiB  
Essay
Scenario Simulation of Glacier Collapse in the Amnye Machen Mountains, Qinghai–Tibetan Plateau
by Jia Li, Junhui Wu, Xuyan Ma, Dongwei Zhou, Long Li, Le Lv, Lei Guo, Lingshuai Kong and Jiahao Dian
Geosciences 2025, 15(7), 254; https://doi.org/10.3390/geosciences15070254 - 3 Jul 2025
Viewed by 396
Abstract
Simulating potential glacier collapses can provide crucial support for local disaster prevention and mitigation efforts. The Xiaomagou Glacier in the Amnye Machen Mountains, Qinghai–Tibetan Plateau, has experienced five collapses in the past two decades. Field investigation and remote sensing observations indicate that the [...] Read more.
Simulating potential glacier collapses can provide crucial support for local disaster prevention and mitigation efforts. The Xiaomagou Glacier in the Amnye Machen Mountains, Qinghai–Tibetan Plateau, has experienced five collapses in the past two decades. Field investigation and remote sensing observations indicate that the topography and bedrock characteristics of the Qushi’an No. 22 Glacier, which is 3.5 km south of the Xiaomagou Glacier, are similar to those of the Xiaomagou Glacier. More importantly, the mass movement of the Qushi’an No. 22 Glacier since 2018 closely resembles that of the Xiaomagou Glacier exhibited before its previous collapses. Therefore, in the context of rising temperatures, it is possible that the Qushi’an No. 22 Glacier will collapse in the near future. Based on remote sensing imagery and the glacier’s surface elevation changes, we reconstructed the 2004 collapse process of the Xiaomagou Glacier via numerical simulation. The key parameters of the mass flow model were optimized based on the actual deposition area of the 2004 collapse. The model with optimized parameters was then used to simulate the potential Qushi’an No. 22 Glacier collapse. Two collapse scenarios were set for the Qushi’an No. 22 Glacier. In Scenario 1, the lower half of the tongue detaches; in Scenario 2, the whole tongue detaches. Simulation results show that, in Scenario 1, the maximum mass flow depth is 72 m, the maximum mass flow speed is 51.6 m/s, and the deposition area is 5.40 × 106 km2; in Scenario 2, the maximum mass flow depth is 75 m, the maximum mass flow speed is 59.7 m/s, and the deposition area is 6.32 × 106 km2. In both scenarios, the deposition area is much larger than that of the Xiaomagou Glacier 2004 collapse, which had a deposition area of 2.21 × 106 km2. The simulation results suggest that the Qushi’an No. 22 Glacier collapse could devastate the pastures and township roads lying in front of the glacier, seriously affecting local transportation and livestock farming; furthermore, it may deposit in the Qinglong River, forming a large, dammed lake. At present, the Qushi’an No. 22 Glacier remains in an unstable state. It is crucial to strengthen monitoring of its surface morphology, flow speed, and elevation. Full article
(This article belongs to the Section Cryosphere)
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13 pages, 1582 KiB  
Article
Design, Analysis, and Verification of a Decoupled Dual-Output Wireless Power Transfer System with a Constant Voltage Output and a Constant Current Output
by Le Yu, You Lv, Xuebin Zhou, Guangyi Yang, Changsong Cai and Lin Yang
Electronics 2025, 14(9), 1706; https://doi.org/10.3390/electronics14091706 - 23 Apr 2025
Viewed by 395
Abstract
Wireless power transfer (WPT) is extensively utilized because of its isolation and convenience. However, WPT systems require different types of outputs in specific applications. The existing dual-receiving WPT system has disadvantages such as redundant compensation components, unnecessary cross-coupling interference, complex control circuits, and [...] Read more.
Wireless power transfer (WPT) is extensively utilized because of its isolation and convenience. However, WPT systems require different types of outputs in specific applications. The existing dual-receiving WPT system has disadvantages such as redundant compensation components, unnecessary cross-coupling interference, complex control circuits, and low space utilization. To address these shortcomings, this paper proposes a dual-output WPT system capable of delivering both constant voltage (CV) and constant current (CC) outputs simultaneously. The WPT system utilizes a special coil structure design, in which the DD coil is used as the transmitter coil and the first receiver coil, and the Q coil is used as the relay coil and the second receiver coil. In addition, the DD coil and the Q coil can achieve natural decoupling under alignment conditions. Consequently, the impact of the unnecessary cross-coupling within the system can be eradicated. Initially, the natural decoupling properties of the magnetic coupler are analyzed from a theoretical perspective. Subsequently, a mathematical model for the proposed WPT system is constructed. Through theoretical deductions, it is demonstrated that under the zero-phase-angle (ZPA) conditions, the system is capable of attaining a CC output at the first receiving side and a CV output at the second receiving side. Finally, a verification experimental prototype with a constant voltage output of 72 V and a constant current output of 2.5 A was built. The experimental results verified the accuracy of the previous theoretical analysis. Full article
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14 pages, 3606 KiB  
Article
Numerical Analysis of Grouting Reinforcement Effects on Deep Foundation Pits Adjacent to Elevated Railways
by Guofei Zhu, Xianlong Wu, Xuhui Li, Le Chang, Yongjie Li, Chuang Lv, Rui Wang and Yingpeng Li
Sustainability 2024, 16(22), 9984; https://doi.org/10.3390/su16229984 - 15 Nov 2024
Viewed by 1005
Abstract
To mitigate the impact of foundation pit construction on adjacent existing structures, grouting reinforcement techniques are often employed to enhance the deformation strength of the soil. This study focuses on the expansion project of the Dayun Comprehensive Hub in Shenzhen, conducting full-scale numerical [...] Read more.
To mitigate the impact of foundation pit construction on adjacent existing structures, grouting reinforcement techniques are often employed to enhance the deformation strength of the soil. This study focuses on the expansion project of the Dayun Comprehensive Hub in Shenzhen, conducting full-scale numerical simulations of the excavation of deep foundation pits adjacent to existing elevated railways and examining the effects of different grouting reinforcement schemes. The results indicate that the single-row and double-row grouting schemes increased the bearing capacity of the foundation piles by 23.7% and 31.9%, respectively, significantly enhancing the structural bearing performance. After reinforcement, the maximum deformation position of the elevated bridge foundation piles shifted upward, and the settlement distribution of the cap beam became more concentrated, indicating that grouting reinforcement effectively controlled the ground settlement and the deformation of the foundation piles. Furthermore, compared to controlling the deformation of the retaining structures, grouting reinforcement was more effective in controlling ground settlement and pile deformation, highlighting its advantages in complex environments. Although the double-row grouting scheme demonstrated superior technical performance, the single-row scheme remains the preferred option considering reinforcement efficiency and economic factors. Full article
(This article belongs to the Special Issue Sustainable Development and Analysis of Tunnels and Underground Works)
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15 pages, 3230 KiB  
Article
A Semi-Analytical Solution of Two-Dimensional Unsteady Groundwater Flow Outside a Long-Strip Excavation Pit with a Cut-Off Wall
by Yinsheng Chang, Le Lv, Xudong Wang and Jin Xu
Appl. Sci. 2024, 14(16), 7367; https://doi.org/10.3390/app14167367 - 21 Aug 2024
Cited by 2 | Viewed by 1074
Abstract
In deep excavation engineering, the implementation of cut-off walls stands as a crucial measure to ensure structural support stability. However, the existing theories for dewatering design often overlook the variations in cut-off wall penetration depths, potentially compromising the efficacy of groundwater control strategies. [...] Read more.
In deep excavation engineering, the implementation of cut-off walls stands as a crucial measure to ensure structural support stability. However, the existing theories for dewatering design often overlook the variations in cut-off wall penetration depths, potentially compromising the efficacy of groundwater control strategies. Addressing this gap, this study conducts a comprehensive investigation into the dynamics of groundwater levels within confined aquifers during the dewatering process of strip excavation pits with cut-off walls. Central to this inquiry is the conceptualization of the entire excavation pit as a singular large-diameter well, with the open part beneath the cut-off wall in the confined aquifer serving as a constant-flux boundary. Employing an advanced analytical modeling approach, the study formulates a robust framework to describe the intricate interplay of unsteady groundwater flow phenomena. Leveraging the techniques of the Laplace and finite cosine transform methods, a semi-analytical solution is derived to elucidate groundwater drawdown patterns over time. The validation of the proposed solution against finite element method results underscores its fidelity and applicability. The parametric analysis reveals a dynamic evolution in drawdown characteristics within the confined aquifer, transitioning from initially cone-shaped distributions to more linear profiles that eventually stabilize with prolonged dewatering. This evolution is governed by the aquifer’s inherent anisotropy and the barrier effect exerted by the depth of cut-off wall penetration. The parametric research also underscores the critical role of lateral boundary distance in influencing groundwater drawdown patterns. The presented solutions can be used to identify optimal penetration depth ratios tailored to specific parameters, thereby offering insights for optimizing dewatering strategies for deep excavation groundwater control. Moreover, a case study was included in this study using the proposed analytical solution, and a comparison was made with field data to validate the practical applicability of the approach. Full article
(This article belongs to the Special Issue Land Subsidence: Monitoring, Prediction and Modeling - 2nd Edition)
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14 pages, 2492 KiB  
Article
Quality and Quantity of School Lunch in Nanjing: Based on Data from the Sunshine Restaurant Supervision Platform
by Xiaofang Lin, Yuanyuan Li, Qiong Wu, Yizhou Lv, Yirong Zhu, Jingwen Liu, Le He and Zhixu Wang
Nutrients 2024, 16(14), 2184; https://doi.org/10.3390/nu16142184 - 9 Jul 2024
Cited by 1 | Viewed by 1773
Abstract
School lunch plays an important role in children’s healthy growth. Previous investigations revealed many problems with school lunches, including unreasonable dietary structure and insufficient micronutrients. This study aimed to assess the dietary structure and nutritional quality of lunches in Nanjing primary and middle [...] Read more.
School lunch plays an important role in children’s healthy growth. Previous investigations revealed many problems with school lunches, including unreasonable dietary structure and insufficient micronutrients. This study aimed to assess the dietary structure and nutritional quality of lunches in Nanjing primary and middle schools. A stratified cluster random sampling method was used to select 44 schools that supply lunch in 12 districts in Nanjing, with two primary and two middle schools in each district. Twenty-four primary and twenty middle schools were selected. The Mann–Whitney U test was used to explore the influencing factors. Findings revealed a serious shortage of milk and fruit in school lunches; supply of eggs, fish, shrimp, and shellfish was less than half of the recommended quantity; livestock and poultry supply exceeded the recommended level by over four times. Energy and nutrition intake were suboptimal. Provision of energy, carbohydrates, vitamins (A, B1, B2, and C), calcium, and iron in urban primary schools was significantly higher than that in non-urban primary schools. The same pattern of significantly higher nutrients was equally seen in urban middle schools compared with non-urban middle schools, indicating that food supply was affected by regional economies. Therefore, it is urgent to improve the quality of lunches, with a particular focus on those in non-urban areas. Full article
(This article belongs to the Section Nutrition Methodology & Assessment)
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12 pages, 6540 KiB  
Article
Realization of Large Low-Stress Elastocaloric Effect in TiZrNbAl Alloy
by Bang-He Lv, Hua-You Xiang, Shang Gao, Yan-Xin Guo, Jin-Han Yang, Nai-Fu Zou, Xiaoli Zhao, Zongbin Li, Bo Yang, Nan Jia, Hai-Le Yan and Liang Zuo
Materials 2024, 17(4), 885; https://doi.org/10.3390/ma17040885 - 14 Feb 2024
Cited by 3 | Viewed by 2303
Abstract
Seeking novel high-performance elastocaloric materials with low critical stress plays a crucial role in advancing the development of elastocaloric refrigeration technology. Here, as a first attempt, the elastocaloric effect of TiZrNbAl shape memory alloy at both room temperature and finite temperatures ranging from [...] Read more.
Seeking novel high-performance elastocaloric materials with low critical stress plays a crucial role in advancing the development of elastocaloric refrigeration technology. Here, as a first attempt, the elastocaloric effect of TiZrNbAl shape memory alloy at both room temperature and finite temperatures ranging from 245 K to 405 K, is studied systematically. Composition optimization shows that Ti-19Zr-14Nb-1Al (at.%), possessing excellent room-temperature superelasticity with a critical stress of around 100 MPa and a small stress hysteresis of around 70 MPa and outstanding fracture resistance with a compressive strain of 20% and stress of 1.7 GPa, demonstrates a substantial advantage as an elastocaloric refrigerant. At room temperature, a large adiabatic temperature change (ΔTad) of −6.7 K is detected, which is comparable to the highest value reported in the Ti-based alloys. A high elastocaloric cyclic stability, with almost no degradation of ΔTad after 4000 cycles, is observed. Furthermore, the sizeable elastocaloric effect can be steadily expanded from 255 K to 395 K with a temperature window of as large as 140 K. A maximum ΔTad of −7.9 K appears at 355 K. The present work demonstrates a promising potential of TiZrNbAl as a low critical stress and low hysteresis elastocaloric refrigerant. Full article
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16 pages, 15408 KiB  
Article
The Dynamic Mechanical Properties and Damage Constitutive Model of Ultra-High-Performance Steel-Fiber-Reinforced Concrete (UHPSFRC) at High Strain Rates
by Xiao Lv, Yan Li, Hui Guo, Wenbiao Liang, Yue Zhai and Le Li
Materials 2024, 17(3), 703; https://doi.org/10.3390/ma17030703 - 1 Feb 2024
Cited by 3 | Viewed by 1554
Abstract
A high strain rate occurs when the strain rate exceeds 100 s−1. The mechanical behavior of materials at a high strain rate is different from that at middle and low strain rates. In order to study the dynamic compressive mechanical properties [...] Read more.
A high strain rate occurs when the strain rate exceeds 100 s−1. The mechanical behavior of materials at a high strain rate is different from that at middle and low strain rates. In order to study the dynamic compressive mechanical properties of ultra-high-performance steel-fiber-reinforced concrete (UHPSFRC) at high strain rates, an electro-hydraulic servo universal testing machine and a separate Hopkinson pressure bar (SHPB) with a diameter of 120 mm were used, respectively. A quasi-static compression test (strain rate 0.001 s−1) and impact compression test with a strain rate range of 90~200 s−1 were carried out to study the failure process, failure mode, and stress–strain curve characteristics of UHPSFRC at different strain rates and quantify the strain rate strengthening effect and fiber toughening effect. Based on the statistical damage theory and energy conversion principle, a dynamic damage constitutive model considering the effects of strain rate and fiber content was constructed. The results showed that the rate correlation of UHPSFRC and the fiber toughening properties showed a certain coupling competition mechanism. When the fiber content was less than 1.5%, with an increase in the steel fiber content, the crack initiation and propagation time of the specimen was extended, and the strain rate sensitivity gradually decreased. When the fiber content was 2%, the impact compressive strength of the specimen was optimal. Compared with UHPC, the dynamic increase factor (DIF) of UHPSFRC was significantly lower. The dynamic damage constitutive model established in this paper, considering the influence of strain rate and fiber content, has a good applicability and can describe the mechanical behavior of UHPSFRC at a high strain rate. Full article
(This article belongs to the Section Construction and Building Materials)
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19 pages, 9078 KiB  
Article
Genome-Wide Identification and Expression Analysis of Auxin Response Factor (ARF) Gene Family in Betula pendula
by Huaizhi Mu, Xuhong Jin, Songtong Lv, Sheng Long, Yang Liu, Le Chen and Lin Lin
Horticulturae 2024, 10(1), 27; https://doi.org/10.3390/horticulturae10010027 - 28 Dec 2023
Cited by 2 | Viewed by 1802
Abstract
As the key transcription factors regulating auxin responsive genes expression, auxin response factors (ARFs) play critical roles in diverse aspects of plant growth and development. Betula pendula is a valuable ornamental tree, and the information on ARF gene family of B. pendula is [...] Read more.
As the key transcription factors regulating auxin responsive genes expression, auxin response factors (ARFs) play critical roles in diverse aspects of plant growth and development. Betula pendula is a valuable ornamental tree, and the information on ARF gene family of B. pendula is needed for better understanding. The publication of the genome sequence of B. pendula enable to analyze the bioinformatics information and expression pattern of BpeARF gene family on the genome-wide basis. In this study, physical and chemical properties, chromosome location, phylogenetic relationship, gene structure, conserved domain, motif composition, and cis-acting element of BpeARF gene family were analyzed, and expression patterns of BpeARF genes were investigated using completely random design in different tissues and under exogenous NAA and drought treatments. A total of 17 BpeARF genes was identified from B. pendula genome, which were unevenly distributed on 13 chromosomes and encoded adequate proteins ranging from 613 to 1135 amino acids in length. Three BpeARF gene pairs were formed by segmental duplication, and the Ka/Ks values of these BpeARF gene pairs were less than 1. According to the phylogenetic relationship among B. pendula, Betula platyphylla, Populus trichocarpa, and Arabidopsis thaliana, the BpeARF genes were divided into four classes, and the intron/exon structure, conserved domain, and motif composition showed high similarity among the BpeARF genes within the same class. The cis-acting elements in the promoter regions of BpeARF genes were related to tissue development, hormone response, and stress resistance. Quantitative real-time PCR exhibited diverse expression patterns of BpeARF genes in different tissues and in response to exogenous auxin treatment and drought stress. The expressions of one, ten, seven, and three BpeARF genes were the high levels in buds, young leaves, stems, and roots, respectively. Under exogenous NAA treatment, six BpeARF genes in stems and roots were upregulated expression at all timepoints. Under drought stress, BpeARF7 and BpeARF15 were upregulated in stems and roots, and BpeARF5 and BpeARF6 were downregulated in leaves, stems, and roots. Our results provided valuable information for the classification and putative functions of BpeARF gene family, which may be helpful for selecting candidate genes and verifying gene function in the genetic engineering of birch trees in further research. Full article
(This article belongs to the Section Genetics, Genomics, Breeding, and Biotechnology (G2B2))
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16 pages, 3675 KiB  
Article
Genome Survey of Stipa breviflora Griseb. Using Next-Generation Sequencing
by Xiangjun Yun, Jinrui Wu, Bo Xu, Shijie Lv, Le Zhang, Wenguang Zhang, Shixian Sun, Guixiang Liu, Yazhou Zu and Bin Liu
Agriculture 2023, 13(12), 2243; https://doi.org/10.3390/agriculture13122243 - 5 Dec 2023
Cited by 1 | Viewed by 1633
Abstract
Due to climate change and global warming, the frequency of sandstorms in northern China is increasing. Stipa breviflora, a dominant species in Eurasian grasslands, can help prevent desertification from becoming more serious. Studies on S. breviflora cover a wide range of fields. [...] Read more.
Due to climate change and global warming, the frequency of sandstorms in northern China is increasing. Stipa breviflora, a dominant species in Eurasian grasslands, can help prevent desertification from becoming more serious. Studies on S. breviflora cover a wide range of fields. To the best of our knowledge, the present study is the first to sequence, assemble, and annotate the S. breviflora genome. In total, 2,781,544 contigs were assembled, and 2,600,873 scaffolds were obtained, resulting in a total length of 649,849,683 bp. The number of scaffolds greater than 1 kb was 70,770. We annotated the assembled genome (>121 kb), conducted a selective sweep analysis, and ultimately succeeded in assembling the Matk gene of S. breviflora. More importantly, our research identified 26 scaffolds that may be responsible for the drought tolerance of S. breviflora Griseb. In summary, the data obtained regarding S. breviflora will be of great significance for future research. Full article
(This article belongs to the Section Crop Genetics, Genomics and Breeding)
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16 pages, 4731 KiB  
Article
The Influence of Exogenous Nitrogen Input on the Characteristics of Phytolith-Occluded Carbon in the Kandelia obovata Soil System
by Huiming You, Lidi Zheng, Weibin You, Fanglin Tan, Fangyi Wang, Yan Cao, Tongchao Le, Jie Lin and Jiangrong Lv
Forests 2023, 14(11), 2202; https://doi.org/10.3390/f14112202 - 6 Nov 2023
Cited by 3 | Viewed by 1657
Abstract
Phytolith-occluded Ccarbon (PhytOC) is an important carbon sink in wetland ecosystems and a mechanism for long-term carbon sequestration. In recent years, nitrogen pollution has become increasingly severe and poses a threat to the healthy development of coastal ecological environments and socio-economic development; therefore, [...] Read more.
Phytolith-occluded Ccarbon (PhytOC) is an important carbon sink in wetland ecosystems and a mechanism for long-term carbon sequestration. In recent years, nitrogen pollution has become increasingly severe and poses a threat to the healthy development of coastal ecological environments and socio-economic development; therefore, studying the impact of nitrogen deposition on the sequestration potential of PhytOC in the soil of coastal wetlands is highly significant. In the present study, two indoor tidal simulation experiments were set up with and without the planting of vegetation. The sequestration capacity and factors that influence soil PhytOC in the Kandelia obovata soil system were compared and analyzed under five nitrogen concentrations. The analysis shows that with the introduction of Kandelia obovata, the occluded carbon content of the soil phytoliths was significantly increased by 31.45% compared with the non-plant group, and the PhytOC content of the soil increased by 7.94%. The exogenous nitrogen input reduced the PhytOC content of the soil, with a rate of decline exceeding 26%. The PhytOC of the soil phytoliths and the PhytOC content of the soil in the planting group increased with increasing nitrogen concentration, while that of the non-plant group decreased as the concentration of nitrogen increased. The non-plant group was more affected by the exogenous nitrogen concentration than the planting group, and the soil microbial biomass carbon and microbial biomass nitrogen were the main factors that influenced changes in the PhytOC. In conclusion, nitrogen input has a significant inhibitory effect on soil PhytOC sequestration potential in coastal wetlands. Planting Kandelia obovata helps to improve the stability of carbon in wetland soil. Full article
(This article belongs to the Special Issue Coastal Forested Wetland Conservation and Carbon Function)
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17 pages, 3655 KiB  
Article
Simulation and Optimization of the Auxiliary Cathode for Inter-Electrode Discharge Electric Field in Microarc Oxidation
by Pengxiang Lv, Xiaozhou Zhang, Lei Chen, Shixuan Wang, Zhen Wang, Rongguo He and Le Guan
Materials 2023, 16(14), 5065; https://doi.org/10.3390/ma16145065 - 18 Jul 2023
Cited by 3 | Viewed by 1582
Abstract
Currently, research on the edge effect issue in the micro-arc oxidation process primarily focuses on investigating process conditions and enhancing additives. However, some scholars have utilized finite element analysis software to simulate the edge effect during the simulation process, overlooking the investigation of [...] Read more.
Currently, research on the edge effect issue in the micro-arc oxidation process primarily focuses on investigating process conditions and enhancing additives. However, some scholars have utilized finite element analysis software to simulate the edge effect during the simulation process, overlooking the investigation of the morphology of the auxiliary cathode. This study analyzes the growth characteristics of the oxide film on aluminum alloy 2A12 during micro-arc oxidation. Additionally, the inter-electrode discharge electric field is simulated using the finite element analysis method. The auxiliary cathode is optimized to mitigate the influence of the edge effect on the film layer. The findings indicate that employing a cylindrical shape as the auxiliary cathode instead of a rectangular groove leads to an increased thickness of the micro-arc oxidation film. However, it also results in an augmented length of the film layer affected by the edge effect at both ends of the workpiece. Decreasing the distance between the auxiliary cathode and the workpiece surface leads to a higher thickness of the obtained micro-arc oxidation film. Decreasing the length of the auxiliary cathode results in a reduction in both the thickness of the film layer on the workpiece surface and the area affected by the edge effect. Increasing the eccentric cone ratio of the auxiliary cathode enhances the uniformity of the micro-arc oxidation film layer. In this study, we present a novel auxiliary cathode model that incorporates a smaller cylindrical shell at the center and eccentric cone shells on each side. This model has the potential to enhance the optimization rate of the micro-arc oxidation film layer on cylindrical workpieces by 17.77%. Full article
(This article belongs to the Special Issue State of the Art: Surface and Coating Technologies)
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17 pages, 4558 KiB  
Article
Antibiofilm Activity and Mechanism of Linalool against Food Spoilage Bacillus amyloliquefaciens
by Guanghui Shen, Lu Yang, Xinyu Lv, Yingfan Zhang, Xiaoyan Hou, Meiliang Li, Man Zhou, Le Pan, Anjun Chen and Zhiqing Zhang
Int. J. Mol. Sci. 2023, 24(13), 10980; https://doi.org/10.3390/ijms241310980 - 1 Jul 2023
Cited by 17 | Viewed by 2833
Abstract
Pellicle biofilm-forming bacteria Bacillus amyloliquefaciens are the major spoilage microorganisms of soy products. Due to their inherent resistance to antibiotics and disinfectants, pellicle biofilms formed are difficult to eliminate and represent a threat to food safety. Here, we assessed linalool’s ability to prevent [...] Read more.
Pellicle biofilm-forming bacteria Bacillus amyloliquefaciens are the major spoilage microorganisms of soy products. Due to their inherent resistance to antibiotics and disinfectants, pellicle biofilms formed are difficult to eliminate and represent a threat to food safety. Here, we assessed linalool’s ability to prevent the pellicle of two spoilage B. amyloliquefaciens strains. The minimum biofilm inhibitory concentration (MBIC) of linalool against B. amyloliquefaciens DY1a and DY1b was 4 μL/mL and 8 μL/mL, respectively. The MBIC of linalool had a considerable eradication rate of 77.15% and 83.21% on the biofilm of the two strains, respectively. Scanning electron microscopy observations revealed that less wrinkly and thinner pellicle biofilms formed on a medium supplemented with 1/2 MBIC and 1/4 MBIC linalool. Also, linalool inhibited cell motility and the production of extracellular polysaccharides and proteins of the biofilm matrix. Furthermore, linalool exposure reduced the cell surface hydrophobicity, zeta potential, and cell auto-aggregation of B. amyloliquefaciens. Molecular docking analysis demonstrated that linalool interacted strongly with quorum-sensing ComP receptor and biofilm matrix assembly TasA through intermolecular hydrogen bonds, hydrophobic contacts, and van der Waals forces interacting with site residues. Overall, our findings suggest that linalool may be employed as a potential antibiofilm agent to control food spoilage B. amyloliquefaciens. Full article
(This article belongs to the Special Issue Biofilm Antimicrobial Strategies: Outlook and Future Perspectives)
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13 pages, 1432 KiB  
Article
Prevalence and Etiological Characteristics of Norovirus Infection in China: A Systematic Review and Meta-Analysis
by Ting-Ting Li, Qiang Xu, Mei-Chen Liu, Tao Wang, Tian-Le Che, Ai-Ying Teng, Chen-Long Lv, Guo-Lin Wang, Feng Hong, Wei Liu and Li-Qun Fang
Viruses 2023, 15(6), 1336; https://doi.org/10.3390/v15061336 - 7 Jun 2023
Cited by 9 | Viewed by 3360
Abstract
Norovirus is a common cause of sporadic cases and outbreaks of gastroenteritis worldwide, although its prevalence and the dominant genotypes responsible for gastroenteritis outbreaks remain obscure. A systematic review was conducted on norovirus infection in China between January 2009 and March 2021. A [...] Read more.
Norovirus is a common cause of sporadic cases and outbreaks of gastroenteritis worldwide, although its prevalence and the dominant genotypes responsible for gastroenteritis outbreaks remain obscure. A systematic review was conducted on norovirus infection in China between January 2009 and March 2021. A meta-analysis and beta-binomial regression model were used to explore the epidemiological and clinical characteristics of norovirus infection and the potential factors contributing to the attack rate of the norovirus outbreaks, respectively. A total of 1132 articles with 155,865 confirmed cases were included, with a pooled positive test rate of 11.54% among 991,786 patients with acute diarrhea and a pooled attack rate of 6.73% in 500 norovirus outbreaks. GII.4 was the predominant genotype in both the etiological surveillance and outbreaks, followed by GII.3 in the etiological surveillance, and GII.17 in the outbreaks, with the proportion of recombinant genotypes increasing in recent years. A higher attack rate in the norovirus outbreaks was associated with age group (older adults), settings (nurseries, primary schools, etc.) and region (North China). The nation-wide pooled positive rate in the etiological surveillance of norovirus is lower than elsewhere in the global population, while the dominant genotypes are similar in both the etiological surveillance and the outbreak investigations. This study contributes to the understanding of norovirus infection with different genotypes in China. The prevention and control of norovirus outbreaks during the cold season should be intensified, with special attention paid to and enhanced surveillance performed in nurseries, schools and nursing homes from November to March. Full article
(This article belongs to the Section Human Virology and Viral Diseases)
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13 pages, 3177 KiB  
Article
Fe-Incorporated Nickel-Based Bimetallic Metal–Organic Frameworks for Enhanced Electrochemical Oxygen Evolution
by Dan Wang, Fuhe Le, Jing Lv, Xue Yang, Xianhao Chen, Haibin Yao and Wei Jia
Molecules 2023, 28(11), 4366; https://doi.org/10.3390/molecules28114366 - 26 May 2023
Cited by 12 | Viewed by 2762
Abstract
Developing cost-effective and high-efficiency catalysts for electrocatalytic oxygen evolution reaction (OER) is crucial for energy conversions. Herein, a series of bimetallic NiFe metal–organic frameworks (NiFe-BDC) were prepared by a simple solvothermal method for alkaline OER. The synergistic effect between Ni and Fe as [...] Read more.
Developing cost-effective and high-efficiency catalysts for electrocatalytic oxygen evolution reaction (OER) is crucial for energy conversions. Herein, a series of bimetallic NiFe metal–organic frameworks (NiFe-BDC) were prepared by a simple solvothermal method for alkaline OER. The synergistic effect between Ni and Fe as well as the large specific surface area lead to a high exposure of Ni active sites during the OER. The optimized NiFe-BDC-0.5 exhibits superior OER performances with a small overpotential of 256 mV at a current density of 10 mA cm−2 and a low Tafel slope of 45.4 mV dec−1, which outperforms commercial RuO2 and most of the reported MOF-based catalysts reported in the literature. This work provides a new insight into the design of bimetallic MOFs in the applications of electrolysis. Full article
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15 pages, 5349 KiB  
Article
Preparation and Application of pH-Sensitive Film Containing Anthocyanins Extracted from Lycium ruthenicum Murr.
by Yucong Zhao, Le Gao, Jing Wang, Ziyan Xue, Mengyao Zhang, Xueli Ma, Guohua Wang and Shenghua Lv
Materials 2023, 16(10), 3828; https://doi.org/10.3390/ma16103828 - 18 May 2023
Cited by 16 | Viewed by 2675
Abstract
A new pH-sensitive film was developed using Artemisia sphaerocephala Krasch. gum (ASKG), soybean protein isolate (SPI), and natural anthocyanin extracted from Lycium ruthenicum Murr. The film was prepared by adsorbing anthocyanins dissolved in an acidified alcohol solution on a solid matrix. ASKG and [...] Read more.
A new pH-sensitive film was developed using Artemisia sphaerocephala Krasch. gum (ASKG), soybean protein isolate (SPI), and natural anthocyanin extracted from Lycium ruthenicum Murr. The film was prepared by adsorbing anthocyanins dissolved in an acidified alcohol solution on a solid matrix. ASKG and SPI were used as the solid matrix for the immobilization of the Lycium ruthenicum Murr. anthocyanin extract, which was absorbed into the film as a natural dye using the facile-dip method. Regarding the mechanical properties of the pH-sensitive film, the tensile strength (TS) values increased approximately 2–5-fold, but the elongation at break (EB) values decreased significantly by about 60% to 95%. With the increase in anthocyanin concentration, the oxygen permeability (OP) values first decreased by about 85%, and then increased by about 364%. The water vapor permeability (WVP) values increased by about 63%, and then decreased by about 20%. Colorimetric analysis of the films revealed variations in color at different pH values (pH 2.0–10.0). Fourier-transform infrared (FT-IR) spectra and XRD patterns indicated compatibility among ASKG, SPI, and anthocyanin extracts. In addition, an application test was conducted to establish a correlation between film color change and carp meat spoilage. At storage temperatures of 25 °C and 4 °C, when the meat was totally spoiled, the TVB-N values reached 99.80 ± 2.53 mg/100 g and 58.75 ± 1.49 mg/100 g, and the film’s color changed from red to light brown and from red to yellowish green, respectively. Therefore, this pH-sensitive film could be used as an indicator to monitor the freshness of meat during storage. Full article
(This article belongs to the Section Biomaterials)
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