Search Results (37)

Current research reported that the number of clinical studies found for botulinum toxin (BoNT) key effects on biochemical biomarkers in head and neck chronic conditions linked to inflammation was very low. There are no systematic reviews of animal studies on this topic, and hence our review aimed to evaluate the quality of the preclinical evidence. We searched PubMed, Scopus, and Web of Science databases, and registries up to 29 January 2024. There were 22 eligible records, and data were available for 11 randomised controlled trials. There were concerns about the risk of bias and great variations of data obtained regarding chronic conditions, which included mostly trigeminal neuralgia. The leading biomarkers were proinflammatory cytokines (IL-1β, TNF-α) and synaptosomal-associated protein-25 (SNAP25), followed by neuron activation marker c-Fos and calcitonin gene-related peptide (CGRP). Overall, data found that BoNT significantly altered the under/over-expression of biomarkers evoked by the investigated disease models and had no effect when the levels of these biomarkers were not changed by the induced chronic conditions in animals. However, there were some mixed results and exceptions, and the certainty evidence found was very low to low. Although the sample sizes detected significant effect size (p < 0.05), most studies are based on male inferior animals, which may limit the recommendations for clinical trials. This study is registered on PROSPERO (CRD42023432411). Full article

Human T-cell lymphotropic virus (HTLV), a retrovirus associated with severe conditions such as leukemia/lymphoma and myelopathy, exhibits variable global prevalence, with higher rates observed in regions such as northeastern Brazil and sub-Saharan Africa. While intrauterine transmission can occur via viral expression in placental tissue and contact with umbilical cord blood, the predominant route is vertical transmission through breastfeeding. Diagnostic testing, particularly serological screening with ELISA and confirmatory methods such as Western blot and PCR, is essential for early detection during pregnancy. The implementation of prenatal screening programs, as seen in Japan and Brazil, has proven effective in reducing vertical transmission by guiding interventions such as breastfeeding cessation in infected mothers. Beyond clinical implications, the psychosocial impact on affected pregnant women highlights the need for an interdisciplinary approach. Although the association between HTLV infection and adverse obstetric outcomes remains controversial, studies suggest increased risks of preterm birth, low birth weight, and other neonatal complications. Given the importance of early diagnosis and prevention, universal prenatal screening protocols represent a critical strategy to reduce viral transmission and its long-term consequences. Full article

Recurrent pregnancy loss (RPL) is a multifactorial condition affecting 1–5% of couples, often with unclear etiology. Idiopathic pregnancy losses (iPLs) are particularly challenging due to unknown molecular mechanisms. This study investigates the transcriptomic profiles of first-trimester products of conception (POC) from iPLs to uncover underlying molecular pathways. We performed RNA-sequencing on nine POC samples, identifying two distinct clusters enriched in trophoblast and decidual cells. Deconvolution analysis revealed reduced syncytiotrophoblast (STB) cells, with increased cytotrophoblast (CTB) and extravillous trophoblast (EVT) cells in iPLs. Gene Set Enrichment Analysis highlighted immune pathways enrichment in both villous trophoblasts and decidua. Gene ontology (GO) analysis of downregulated genes implicated hormonal and endocrine processes, consistent with STB reduction, while upregulated genes were associated with MHC protein complex and immune system processes, aligning with EVT increases. Histological analysis showed chronic histiocytic intervillositis (CHI) in iPL samples, supporting maternal immune dysregulation in unexplained RPL. Together, transcriptomic and histological analyses indicate that immune signaling dysregulation and impaired trophoblast differentiation may underlie unexplained iPLs. These findings bridge molecular and histopathological evidence, underscoring the interplay between trophoblast dysfunction and immune imbalance. Our results provide insights into iPL pathogenesis, highlighting potential biomarkers that may contribute to improved diagnosis and future research. Full article

Background/Objectives: Leadership in nursing plays a pivotal role in the provision of high-quality healthcare. It is, therefore, of paramount importance to understand the challenges encountered by newly qualified nurses when assuming leadership responsibilities. This scoping review protocol aims to identify and map these challenges, thereby facilitating the development of more effective interventions and support programmes. The data collection and analysis were conducted from January 2024 to March 2024. Methods: The review was conducted in accordance with the Joanna Briggs Institute (JBI) guidelines for scoping reviews and the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) recommendations. A comprehensive search was undertaken across MEDLINE, CINAHL, Web of Science, Scopus, LILACS, and SciELO databases, in addition to a search for the grey literature. Study selection was performed by two independent reviewers, applying pre-defined inclusion and exclusion criteria. The data were extracted using a standardised form and analysed descriptively. Results: The review identified several key challenges faced by newly qualified nurses in leadership roles, including conflict management, decision-making under pressure, and effective communication. These findings highlight the complexity of transitioning from academic training to professional practice. The results provide a comprehensive overview of these challenges, enabling the identification of gaps in the existing knowledge base and informing the direction of future research and interventions. Conclusions: The findings of this review will contribute to the enhancement of healthcare quality and the promotion of the professional development of newly qualified nurses. This research underscores the importance of developing targeted interventions and support systems to address the specific challenges identified, thereby influencing nursing practice and policy. Full article

This study compared the efficacy of different solutions in achieving patency in teeth filled with AH Plus Bioceramic sealer. Eighty-five premolars with a straight canal were prepared. After sealer placement, a master gutta-percha cone was introduced 2 mm short of the working length. The teeth were stored at 37 °C and 100% humidity for five weeks before retreatment. Filling materials were removed up to the gutta-percha cone’s length. The canals were then randomly assigned to groups: G1 (control, no solution), G2 (5.25% NaOCl), G3 (17% EDTA), G4 (10% citric acid), and G5 (10% formic acid). The apical patency was attempted with a 10 K file within a period of 10 min, by a blinded operator. Additionally, sealer samples were immersed in the solutions, followed by scanning electron microscopy analysis. The Kruskal–Wallis test was used for statistical analysis. Patency was achieved in all canals except one in the control and one in the NaOCl groups. No significant differences were found in the time required to achieve patency. Acid solutions had a greater impact on the sealer’s structural integrity, and a decalcifying effect of EDTA and citric acid was registered. Apical patency in straight canals obturated with AH Plus Bioceramic sealer was consistently achieved regardless of the solution used. Full article

Background: School ergonomics is a subject of growing interest for the scientific community due to the health problems that it is associated with in students, mainly asymmetries in the spine due to the use of chairs and desks that are inadequate for their anthropometry. This study aimed to analyze the anthropometric characteristics and asymmetries among fifth- to ninth-grade students in Spain and Portugal, with the goal of obtaining data on the ideal height of seats and desks. Additionally, it evaluated the correspondence in the recommended sizes of chairs and desks according to the parameters of the European Union catalog and examined the suitability of the height as a criterion for the allocation of school furniture. Methods: Different anthropometric variables, including the height, popliteal height, shoulder-to-seat height, and elbow-to-seat height, were measured in a stratified sample of 500 students (mean age = 12.7 years, SD = 1.2) across different grades (fifth grade = 86, sixth grade = 106, seventh grade = 95, eighth grade = 89, ninth grade = 124), genders (males = 256, females = 244), and countries (Spain = 191, Portugal = 309). These measurements were used to calculate the average ideal seat and desk heights based on anthropometric formulas, which were then compared to the current furniture allocation practices. The statistical analyses included t-tests, chi-squared tests, and effect sizes, with adjustments for multiple comparisons. Results: The results revealed significant asymmetries and low correspondence in the allocation of chairs and desks of the same sizes to students, with a match rate ranging between 40% and 70%. Moreover, the correspondence was even lower when using a formula based solely on height, compared to formulas validated with specific anthropometric measures, particularly for desks, where the asymmetries reached 100% in some grades. Conclusion: These findings highlight the need to improve the adaptation of school furniture to optimize student ergonomics and comfort, and they suggest disregarding the height as the primary criterion for furniture allocation. Additionally, assigning a desk size based on the recommended chair size is discouraged. Full article

Loop-mediated isothermal amplification (LAMP) is a highly effective molecular diagnostic technique, particularly advantageous for point-of-care (POC) settings. In recent years, LAMP has expanded to include various adaptations such as DARQ-LAMP, QUASR, FLOS-LAMP, displacement probes and molecular beacons. These methods enable multiplex detection of multiple targets in a single reaction, enhancing cost-effectiveness and diagnostic efficiency. Consequently, LAMP has gained significant traction in diagnosing diverse viruses, notably during the COVID-19 pandemic. However, its application for detecting Herpesviridae remains relatively unexplored. This group of viruses is of particular interest due to their latency and potential reactivation, crucial for immunocompromised patients, including organ and hematopoietic stem cell transplant recipients. This review highlights recent advancements in LAMP for virus diagnosis and explores current research trends and future prospects, emphasizing the detection challenges posed by Herpesviridae. Full article

Osteoporosis is a common systemic bone disorder in the elderly, characterized by low bone mineral density and deterioration of bone structure. Apical periodontitis is an inflammatory response to the microbial infection of root canals, typically characterized by apical bone destruction surrounding the tooth’s apex. This systematic review aimed to determine if osteoporosis affects the prevalence of apical periodontitis in adults. PRISMA guidelines have been followed. It included randomized clinical trials, cross-sectional, cohort, and case-control studies, and excluded non-relevant investigations and various secondary sources. A comprehensive search was performed in PubMed, Scopus, and Web of Science, until 13 March 2024. The Newcastle–Ottawa Scale was used to assess the quality of the three selected studies: two cross-sectional studies and one case-control study. One investigation only included post-menopausal women recruited at a dental university clinic, the other integrated data from the total hospital patients’ population, and the third selected patients referred to the university dental clinic from the university hospital. The findings varied: one study noted a marginal association between low bone mineral density and apical periodontitis, another found a significant association, and the third, with the lowest risk of bias, reported no link. The main limitations were the scarcity of eligible studies and their overall quality. The review was registered in the PROSPERO database (CRD42024523705), applied strict inclusion criteria and thorough searches by experienced and independent reviewers. There is no strong evidence that adult individuals with osteoporosis have a higher probability of developing apical periodontitis. However, clinicians should remain cautious of osteoporosis’s potential impact on apical periodontitis development. Full article

Musculoskeletal disorders (MSD) encompass a variety of conditions affecting muscles, joints, and nerves. In Portugal, MSDs are the most prevalent occupational health problem in companies. Based on the relevance of work-related MSD (WMSD), this study aims to assess the prevalence of MSD complaints in a needle manufacturing industry in Northern Portugal, following a cross-sectional approach. Thus, 526 workers from five departments (i.e., operator, tuning, maintenance, administration, and logistics) answered a sociodemographic questionnaire and the Nordic Musculoskeletal Questionnaire (NMQ). Within the last 12 months, females exhibited a higher frequency of complaints than males across all body parts except for ankles/feet. The body parts eliciting the most percentage of complaints for both genders include the lower back (54.2%), neck (42.2%), shoulders (39.0%), ankles/feet (38.2%), and wrists/hands (35.7%). No significant association was found between Body Mass Index (BMI) and body part complaints. Tuners reported the highest complaint rate, with occupations as substantial predictors of complaints in certain body parts. Likewise, complaints tend to increase with age. The findings advocate for ergonomic interventions that are gender-, age-, and job-sensitive. Full article

Sentiment analysis is a cornerstone of natural language processing. However, it presents formidable challenges due to the intricacies of lexical diversity, complex linguistic structures, and the subtleties of context dependence. This study introduces a bespoke and integrated approach to analyzing customer sentiment, with a particular emphasis on a case study in the Portuguese retail market. Capitalizing on the strengths of SentiLex-PT, a sentiment lexicon curated for the Portuguese language, and an array of sophisticated machine learning algorithms, this research constructs advanced models that encapsulate both lexical features and the subtleties of linguistic composition. A meticulous comparative analysis singles out multinomial logistic regression as the pre-eminent model for its applicability and accuracy within our case study. The findings of this analysis highlight the pivotal role that sentiment data play in strategic decision-making processes such as reputation management, strategic planning, and forecasting market trends within the retail sector. To the extent of our knowledge, this work is pioneering in its provision of a holistic sentiment analysis framework tailored to the Portuguese retail context, marking an advancement for both the academic field and industry application. Full article

Background/Objective: To ensure that implants are able to support prosthetic rehabilitation, a stable and functional union between the bone and the implant surface is crucial to its stability and success. To increase bone volume and density and excel bone-implant contact, a novel drilling method, called osseodensification (OD), was performed. To assess the effectiveness of the osseodensification drilling protocol versus the conventional surgical technique on implant stability. Methods: Bone Level Tapered Straumann implants were placed side-by-side with both OD and subtractive conventional drilling (SD) in 90 patients from CESPU—Famalicão clinical unit. IT was measured using a manual torque wrench, and the Implant stability quotient (ISQ) value was registered using the Osstell^® IDX. Results: According to the multifactorial ANOVA, there were statistically significant differences in the mean IT values due to the arch only (F(1.270) = 4.702, p-value = 0.031 < 0.05). Regarding the length of the implant, there were statistically significant differences in the mean IT in the OD group (p = 0.041), with significantly lower mean IT values for the Regular implants compared to the Long. With respect to the arch, the analyses of the overall ISQ values showed an upward trend in both groups in the maxilla and mandible. High levels of IT also showed high ISQ values, which represent good indicators of primary stability. Conclusions: OD does not have a negative influence on osseointegration compared to conventional subtractive osteotomy. Full article

The funerary/cult archeological nucleus of Rua do Raio (Braga, in the northwest of the Iberian Peninsula) was discovered between 2007 and 2009, under the excavation works of a necropolis of Bracara Augusta. This building exhibits a set of particularities that confirms its archeological importance. It is a construction dating from the middle of the 1st century AD, subject to two reforms, one in the second half of the 1st century AD and another in the 2nd century AD, with a trapezoidal shape and comprising ten rectangular tanks rendered with opus signinum mortars. It shows a unique architectural configuration in the city, as well as in the Portuguese territory, and the space is under musealization, together with a set of graves identified in the same archeological intervention. The present investigation contextualizes the funerary and religious architecture of the city. Its description is presented, highlighting its relevance, and an interpretative possibility is formulated. Full article

Inflammatory bowel diseases (IBD) include Crohn’s disease and ulcerative colitis. Several studies relate eating habits to different aspects of IBD, such as progression and worsening of the clinical condition. Therefore, many natural products (NPs) such as polyphenols and carotenoids have been identified as promising agents in supporting IBD. An interesting source for obtaining bioactive NPs is the by-products of the food industry. The present study evaluated the potential beneficial effect of a standardized extract (CAE) obtained from cashew apple bagasse in the dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice. This was the first time that CAE had been evaluated in this experimental model. Chemical evaluation of CAE identified carotenoids (96.28 ± 0.15 mg/100 g), phenolic compounds (37.49 ± 0.64 mg/100 g), and a mixture of anacardic acids (C15:3 = 94.2 ± 0.6 mg/100 g; C15:2 = 108.4 ± 0.1 mg/100 g; C15:1 = 214.8 ± 0.2 mg/100 g). Administration of CAE (500 mg/kg, 4 days, p.o.) after DSS challenge was more effective in delaying disease progression compared with prior treatment (500 mg/kg, 30 days, p.o.), according to the disease activity index. However, no treatment strategy with CAE was able to prevent or inhibit disease progression, since all parameters evaluated (macroscopic, biochemical, and histopathological) in CAE-treated animals were similar to those observed in DSS-challenged animals. Despite the high dose (500 mg/kg), the standardized extract (CAE) did not result in an effective concentration of carotenoids. Furthermore, as some anacardic acids have been reported as histone acetyltransferases inhibitors, there could be a possible antagonistic relationship between carotenoids and anacardic acids. Complementary research will be necessary to test the hypothesis of antagonism. Thus, an optimized extract, with an even higher concentration of carotenoids, obtained from cashew apple bagasse, can be developed as a possible adjuvant food supplement for inflammatory bowel diseases. Full article

Leishmaniasis is a disease caused by protozoa of the genus Leishmania. Treatment options are limited, and there are frequent cases of treatment failure and clinical relapse. To understand these phenomena better, a systematic review was conducted, considering studies published between 1990 and 2021 in Portuguese, English, and Spanish. The review included 64 articles divided into three categories. Case reports (26 articles) focused on treatment failure and clinical relapse in cutaneous leishmaniasis patients (47.6%), primarily affecting males (74%) and children (67%), regardless of the clinical manifestation. Experimental studies on the parasite (19 articles), particularly with L. major (25%), indicated that alterations in DNA and genic expression (44.82%) played a significant role in treatment failure and clinical relapse. Population data on the human host (19 articles) identified immunological characteristics as the most associated factor (36%) with treatment failure and clinical relapse. Each clinical manifestation of the disease presented specificities in these phenomena, suggesting a multifactorial nature. Additionally, the parasites were found to adapt to the drugs used in treatment. In summary, the systematic review revealed that treatment failure and clinical relapse in leishmaniasis are complex processes influenced by various factors, including host immunology and parasite adaptation. Full article

Wildfires can cause serious imbalances in ecosystems, primarily at the soil level, making it vulnerable to degradation processes such as erosion. During and after a fire, changes occur in soil properties, including pH, which affects the solubility and availability of nutrients. Currently, there is a great diversity of protocols, some involving normalized standards, to determine soil pH, but there is no consensual or universal analytical method for this parameter, especially in burned soils, in which mineral and organic fractions could have been modified. Therefore, the objective of the present work is to evaluate the effect that variations in these analytical protocols may have on pH results. For this, five methods commonly found in the international bibliography for the analysis of pH of soil in water (pH_H2O) were selected and compared to propose the most precise procedure. The analytical methods were applied to 43 soil samples, collected in a plot subjected to prescribed burning in the Parque Natural de Montesinho (Northern Portugal). The studied methods differ in the following protocol items: water suspension ratio (1:2.5 or 1:5), mechanical stirring time in the suspension (10 min or 1 h), and in the resting time for the solid particles to settle (15 min or 8 h). The obtained results point to the suitability of the five methods used for soil pH analysis, indicating that there are no statistically significant differences. However, results also allow suggesting a more appropriate method concerning practical reasons, such as labor in a lab. Thus, to make the analysis process more profitable, M2 is a good option because it uses a small amount of sample (5 g), short agitation (10 min) and settling time (15 min). In turn, M1 and M5, which use a lower proportion of soil (1:2.5) show lower pH variation in the measurements. This fact may be explained by a smaller dilution effect. Considering that these two methods differ in the settling time, it is suggested to apply M1, because only 15 min are required. Therefore, the main conclusion reached with this work is that the measurement of soil pH using M1, i.e., a soil:water ratio of 1:2.5, with agitation of 10 min and settling time of 15 min, is a robust and more expeditious protocol to be applied to soil samples after a fire. Full article