Virus Biosensing

A special issue of Viruses (ISSN 1999-4915). This special issue belongs to the section "General Virology".

Deadline for manuscript submissions: 30 November 2026 | Viewed by 3361

Special Issue Editor


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Guest Editor
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China
Interests: design and develop a variety of fluorescence analysis technologies for the early diagnosis of diseases and answer basic scientific questions in virology; synthetic biosensors for disease diagnosis and food safety; design and synthesis of fluorescence nanoprobes; virus labelling and tracking

Special Issue Information

Dear Colleagues,

The outbreak of infectious viruses presents one of the greatest threats to public healthcare systems. Controlling the spread of these viruses continues to be a global challenge. Early and accurate virus diagnosis is crucial for preventing virus transmission, especially when lacking a specific vaccine or effective medicine. Several methods have been constructed for viral detection in clinics, including molecular diagnosis and immunoassays for measuring nucleic acids or protein biomarkers. However, most molecular diagnosis-based methods require complex infrastructure and expensive equipment, which are not suitable for resource-limited settings. Most immunoassays, such as lateral flow assays, show high speed, but their sensitivity is limited for accurate diagnosis. Various new emerging diagnosis technologies, including CRISPR/Cas system, DNA nanotechnology, microfluidics, and smartphone-based point-of-care testing, have been developed to enhance sensitivity, speed, specificity, and portability.

Therefore, we invite researchers from around the world to submit original research and review articles focused on emerging methods for virus detection, including nucleic acid amplification, CRISPR-based detection, DNA nanotechnology, point-of-care testing, etc.

Dr. Guobin Mao
Guest Editor

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Keywords

  • virus detection
  • molecular diagnosis
  • immunoassay
  • nucleic acid amplification
  • CRISPR-based detection
  • nucleic acid assembly
  • biosensor
  • point-of-care testing

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Published Papers (2 papers)

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Research

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14 pages, 1252 KB  
Article
Metatranscriptomic Analysis of Oropharyngeal Samples Reveals Common Respiratory Viruses and a Potential Interspecies Transmitted Picobirnavirus in the Wayuu Population, La Guajira, Colombia
by Beatriz Elena De arco-Rodríguez, Jhindy Tatiana Pérez-Lozada, Katherine Laiton-Donato, Dioselina Peláez-Carvajal, Gloria Mercedes Puerto-Castro and Diego Alejandro Álvarez-Díaz
Viruses 2025, 17(10), 1397; https://doi.org/10.3390/v17101397 - 21 Oct 2025
Viewed by 1572
Abstract
Acute respiratory infections and other infectious diseases causing acute febrile syndrome are major public health concerns in Colombia, particularly among vulnerable populations such as the Wayuu Indigenous community in Manaure, La Guajira. To investigate their viral etiology, 55 nasopharyngeal swabs and 58 serum [...] Read more.
Acute respiratory infections and other infectious diseases causing acute febrile syndrome are major public health concerns in Colombia, particularly among vulnerable populations such as the Wayuu Indigenous community in Manaure, La Guajira. To investigate their viral etiology, 55 nasopharyngeal swabs and 58 serum samples were collected from febrile Wayuu individuals in Manaure. RT-qPCR screening identified Coronavirus, Enteroviruses, Adenovirus, and Influenza A/B in respiratory samples, while no arboviruses were detected in serum. Sixteen representative samples underwent metatranscriptomic next-generation sequencing (mtNGS) using the Chan-Zuckerberg ID (CZ-ID) platform. This analysis confirmed RT-qPCR findings and additionally revealed six viral contigs related to Orthopicobirnavirus hominis. Sequencing coverage enabled the reconstruction of a consensus RdRp segment, which was phylogenetically compared with sequences from diverse hosts. The virus clustered within genogroup 1, alongside Colombian isolates linked to severe acute respiratory infection. The absence of strict host-specific clustering suggests possible interspecies transmission. These findings underscore the complementary roles of targeted and unbiased approaches: RT-qPCR detected common respiratory viruses, whereas mtNGS uncovered a virus previously unreported in this community. Overall, mtNGS emerges as a powerful tool to support viral surveillance and provide baseline evidence in indigenous populations, emphasizing the need to decentralize advanced molecular diagnostics and strengthen public health capacity in Colombia. Full article
(This article belongs to the Special Issue Virus Biosensing)
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Review

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21 pages, 1495 KB  
Review
CRISPR-Based Detection of Viral Hemorrhagic Fevers at the Point of Care
by Kylene Wupori, Lauren Garnett, Alexander Bello and James E. Strong
Viruses 2026, 18(2), 218; https://doi.org/10.3390/v18020218 - 7 Feb 2026
Viewed by 1125
Abstract
Viral hemorrhagic fevers (VHFs) are highly lethal diseases that often present non-specific, influenza-like symptoms in their early stages, making clinical recognition and differentiation from other febrile illnesses difficult. This overlap underscores the critical need for diagnostic tests that are both sensitive and specific. [...] Read more.
Viral hemorrhagic fevers (VHFs) are highly lethal diseases that often present non-specific, influenza-like symptoms in their early stages, making clinical recognition and differentiation from other febrile illnesses difficult. This overlap underscores the critical need for diagnostic tests that are both sensitive and specific. Point-of-care (POC) diagnostic tests are an invaluable tool for detecting and controlling the spread of pathogens that threaten public health, such as VHFs, as these require fast, accurate diagnostics to ensure biosafety and appropriate mobilization of resources during outbreaks. Current molecular and serological diagnostic tests, while efficient and effective, lack the characteristics required of a POC test (POCT) to quickly and easily respond to a VHF outbreak while maintaining a low cost. Clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostic tests have gained popularity as POCTs due to their inherent attractive qualities, including high sensitivity and specificity, adaptability, low cost, quick turnaround time, and ease of use. However, studies on the development of CRISPR-based POC diagnostic tests for VHFs are limited. This review summarizes the current CRISPR-based POCTs for VHFs, including Ebola virus (EBOV), Lassa virus (LASV), Dengue virus (DENV), and Crimean–Congo hemorrhagic fever virus (CCHF). The isothermal pre-amplification methods commonly paired with CRISPR-based tests, such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), are also discussed. Full article
(This article belongs to the Special Issue Virus Biosensing)
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