Developmental Biology and Genetics in Chicken Embryo Germ Cell

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Animal Genetics and Genomics".

Deadline for manuscript submissions: closed (25 March 2025) | Viewed by 467

Special Issue Editors

Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Interests: chicken embryo development; avian germline stem cells; sex determination

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Guest Editor
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
Interests: chicken germ cell development; chicken embryo development; animal embryo engineering

Special Issue Information

Dear Colleagues,

The chicken embryo is an important model in research, contributing to groundbreaking concepts in developmental biology and genetics. With recent advances in molecular genetics and omics technologies, significant progress has been made in uncovering the complex genomic characteristics of chicken embryo germ cells. Diverse molecular and omics technologies, such as the CRISPR/Cas9 gene editing system, PacBio or Oxford Nanopore sequencing, scRNA-seq, spatial transcriptomics, and synthetic biology, provide robust tools for studying the intricate genomic characteristics and regulatory networks within these cells. These comprehensive analyses reveal the genetic mechanisms governing germ cell development, differentiation, and function, offering crucial insights into avian reproductive biology. In this Special Issue, we aim to highlight the application of these innovative technologies in the study of chicken embryo germ cell development. We welcome studies that utilize gene editing systems, omics technologies, experimental validation, and other innovative approaches to investigate the developmental and molecular mechanisms of chicken germ cells. Both theoretical and empirical contributions that enhance our understanding of the genetic factors influencing germ cell biology are encouraged.

Dr. Kai Jin
Dr. Yingjie Niu
Guest Editors

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Keywords

  • chicken embryo
  • germ cell
  • developmental biology
  • genetics
  • omics
  • experimental validation

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Published Papers (1 paper)

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Research

18 pages, 5921 KiB  
Article
Functional Equivalence of Insulin and IGF-1 in the In Vitro Culture of Chicken Primordial Germ Cells
by Xin Liu, Jun Wu, Yixiu Peng, Guangzheng Liu, Kai Jin, Yingjie Niu, Jiuzhou Song, Wei Han, Guohong Chen, Bichun Li and Qisheng Zuo
Genes 2025, 16(5), 481; https://doi.org/10.3390/genes16050481 - 24 Apr 2025
Viewed by 200
Abstract
Background: Chicken Primordial Germ Cells (PGCs) are one of the few germ cells that can be cultured for a long time in vitro, but challenges remain such as low culture efficiency and unclear roles of nutrient factors and signaling pathways. Method: In this [...] Read more.
Background: Chicken Primordial Germ Cells (PGCs) are one of the few germ cells that can be cultured for a long time in vitro, but challenges remain such as low culture efficiency and unclear roles of nutrient factors and signaling pathways. Method: In this study, protein kinase B (AKT) pathway activator insulin-like growth factor 1 (IGF-1) was screened for its ability to promote cell proliferation by transcriptome results using various inhibitors of pathway activation. The effects of IGF-1 on PGCs were evaluated through EdU assays, qRT-PCR, flow cytometry, and migration experiments. Results: This study systematically examined the effects of insulin and IGF-1 on the proliferation, cell cycle, ferroptosis, migration capacity, and establishment efficiency of PGCs. The findings demonstrated that IGF-1 exhibited comparable effects to insulin and could effectively replace insulin in PGC culture systems. Conclusions: The research results are expected to provide a solid theoretical basis for optimizing the chicken PGC cultivation system and promoting its practical application. Full article
(This article belongs to the Special Issue Developmental Biology and Genetics in Chicken Embryo Germ Cell)
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