CRISPR-Cas Based Genome Editing and Transcription Control
A special issue of BioTech (ISSN 2673-6284).
Deadline for manuscript submissions: closed (30 April 2020) | Viewed by 188
Special Issue Information
Dear Colleagues,
The clustered regularly interspaced short palindromic repeats (CRISPR)–Cas (CRISPR-associated proteins) system is a programmable RNA-guided endonuclease that has allowed the facile introduction of genomic/transcriptomic alterations and furnished “disruptive” technologies. The simplicity and broad applicability of these guided genome editing methods foster new therapeutic modalities to common diseases as well as rare genetic diseases. Catalytically dead-Cas (dCas) or nickase-Cas (nCas) molecules are fused with important epigenetic modifiers, transcription factors, cytosine deaminase (ApoBEC) have allowed a loci-specific modulation of transcription control, avoiding global off-target effects. Despite the increasing research on basic and therapeutic aspects of CRISPR–Cas genome editing, several questions and puzzles remain unsolved, and include: size and packaging of these proteins, spatio-temporal control of genome editing, global vs. local transcription, off-target effects, low knock-in efficiency, immunogenicity, pre-existing immunity, different cell type targeting, scalability of production, and quality control.
To further understand this exciting area, in this Special Issue, we invite manuscripts regarding methods for CRISPR–Cas-based gene knock-out and/or knock-in studies, base-editing methods, high-throughput pooled CRISPR screens, analyzing high-throughput off-target data, Cas9-based transcription control methods, high-throughput screening methods for the control of genome editing, the importance of antiCRISPR molecules in the outcome of genome editing, therapeutic ex vivo and in vivo genome editing, delivery methods for genome-editing proteins, and CRISPR-based animal models for understanding of disease pathology and therapy.
Dr. Sreekanth Vedagopuram
Guest Editor
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Keywords
- CRISPR–Cas genome editing
- base editing
- transcription control
- gene regulation
- anti-CRISPR molecules
- ex vivo therapeutic genome editing
- double strand break repair
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