Formation and Electrochemical Evaluation of Polyaniline and Polypyrrole Nanocomposites Based on Glucose Oxidase and Gold Nanostructures

Nanocomposites based on two conducting polymers, polyaniline (PANI) and polypyrrole (Ppy), with embedded glucose oxidase (GOx) and 6 nm size gold nanoparticles (AuNPs_(6nm)) or gold-nanoclusters formed from chloroaurate ions (AuCl₄⁻), were synthesized by enzyme-assisted polymerization. Charge (electron) transfer in systems based on PANI/AuNPs_(6nm)-GOx, PANI/AuNPs_(AuCl4⁻₎-GOx, Ppy/AuNPs_(6nm)-GOx and Ppy/AuNPs_(AuCl4⁻₎-GOx nanocomposites was investigated. Cyclic voltammetry (CV)-based investigations showed that the reported polymer nanocomposites are able to facilitate electron transfer from enzyme to the graphite rod (GR) electrode. Significantly higher anodic current and well-defined red-ox peaks were observed at a scan rate of 0.10 V s⁻¹. Logarithmic function of anodic current (log I_pa), which was determined by CV-based experiments performed with glucose, was proportional to the logarithmic function of a scan rate (log v) in the range of 0.699–2.48 mV s⁻¹, and it indicates that diffusion-controlled electrochemical processes were limiting the kinetics of the analytical signal. The most efficient nanocomposite structure for the design of the reported glucose biosensor was based on two-day formed Ppy/AuNPs_(AuCl4⁻₎-GOx nanocomposites. GR/Ppy/AuNPs_(AuCl4⁻₎-GOx was characterized by the linear dependence of the analytical signal on glucose concentration in the range from 0.1 to 0.70 mmol L⁻¹, the sensitivity of 4.31 mA mM cm⁻², the limit of detection of 0.10 mmol L⁻¹ and the half-life period of 19 days.