New Polyphenol-Containing LDL Nano-Preparations in Oxidative Stress and DNA Damage: A Potential Route for Cell-Targeted PP Delivery
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Isolation and Purification of LDL
2.3. DLS Particle Size Measurement
2.4. Cell Culture Conditions
2.5. Neutral Red Uptake (NRU) Assay of Cell Metabolic Activity
2.6. Determination of the Level of Reactive Oxygen Species in a 2780 Human Ovarian Cancer Cells
2.7. DiL Staining of Lipoproteins
2.8. LDL Binding Assay
2.9. Plasmid Nicking Assay
3. Results
3.1. Influence of PP Saturation on the Size of the LDL Particle
3.2. Toxicity
3.3. LDL Binding Affinity Test
3.4. The Level of the Reactive Oxygen Species
3.5. Plasmid Nicking Assay
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Polyphenol | Water Solubility g/L | logP |
---|---|---|
Curcumin | 0.00575 | 3.62 |
Quercetin | 0.261 | 1.81 |
Chlorogenic acid | 3.44 | 0.17 |
Polyphenol | Max (nm) | ε (mol−1cm−1) |
---|---|---|
Q | 372 | 17864 |
Cur | 422 | 52472 |
CLA | 329 | 17971 |
Preparation | Average Diameter (nm) | Main Diameter (nm) | PDI |
---|---|---|---|
LDL | 28.9 | 23.4 | 0.159 |
QLDL | 29.7 | 23.0 | 0.195 |
CurLDL | 29.6 | 24.5 | 0.185 |
CLALDL | 30.9 | 22.8 | 0.195 |
PP | Concentration (2 h, µM) | Concentration (11 h, µM) |
---|---|---|
Q | 8.4 | 69.2 |
Cur | 31.8 | 87.9 |
CLA | 0 | 0 |
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Lewandowska, H.; Kalinowska, M. New Polyphenol-Containing LDL Nano-Preparations in Oxidative Stress and DNA Damage: A Potential Route for Cell-Targeted PP Delivery. Materials 2020, 13, 5106. https://doi.org/10.3390/ma13225106
Lewandowska H, Kalinowska M. New Polyphenol-Containing LDL Nano-Preparations in Oxidative Stress and DNA Damage: A Potential Route for Cell-Targeted PP Delivery. Materials. 2020; 13(22):5106. https://doi.org/10.3390/ma13225106
Chicago/Turabian StyleLewandowska, Hanna, and Monika Kalinowska. 2020. "New Polyphenol-Containing LDL Nano-Preparations in Oxidative Stress and DNA Damage: A Potential Route for Cell-Targeted PP Delivery" Materials 13, no. 22: 5106. https://doi.org/10.3390/ma13225106