Comparison of Ultraviolet A/B and C Irradiation for Exosome Secretion Enhancement in HEK 293T Cell †
Abstract
1. Introduction
2. Materials and Method
2.1. Cell Culture
- Day 1: Cells were planted at a density of 1 × 107 cells/mL in 10 cm dishes using DMEM medium supplemented with penicillin/streptomycin (P/S) and 10% fetal bovine serum (FBS); triple-plates cells were reserved for the control (Ct).
- Day 2: Irradiating the HEK-293T cells with ultraviolet A/B (UVP, CL-1000 Ultraviolet Crosslinker, Upland, CA, USA) and ultraviolet C (UVP, CL-1000 Ultraviolet Crosslinker, USA) light for 10/30/60 min; triple repeat. Then, the dishes were transferred to a cell culture incubator set at 37 °C with 5% CO2 for 24 h.
- Day 3: The conditioned medium was collected from the dishes.
2.2. Cell Count and Cell Morphology
2.3. Exosome Concentration
2.4. Bradford Assay
2.5. Nanoparticle Tracking Analysis (NTA)
2.6. Western Blot
3. Results
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chan, C.-C.; Lin, P.; Xian, Y.; Chou, R.-H.; Liu, Y.-J. Comparison of Ultraviolet A/B and C Irradiation for Exosome Secretion Enhancement in HEK 293T Cell. Eng. Proc. 2025, 89, 39. https://doi.org/10.3390/engproc2025089039
Chan C-C, Lin P, Xian Y, Chou R-H, Liu Y-J. Comparison of Ultraviolet A/B and C Irradiation for Exosome Secretion Enhancement in HEK 293T Cell. Engineering Proceedings. 2025; 89(1):39. https://doi.org/10.3390/engproc2025089039
Chicago/Turabian StyleChan, Ching-Chih, Pohao Lin, Yi Xian, Ruey-Hwang Chou, and Yi-Jui Liu. 2025. "Comparison of Ultraviolet A/B and C Irradiation for Exosome Secretion Enhancement in HEK 293T Cell" Engineering Proceedings 89, no. 1: 39. https://doi.org/10.3390/engproc2025089039
APA StyleChan, C.-C., Lin, P., Xian, Y., Chou, R.-H., & Liu, Y.-J. (2025). Comparison of Ultraviolet A/B and C Irradiation for Exosome Secretion Enhancement in HEK 293T Cell. Engineering Proceedings, 89(1), 39. https://doi.org/10.3390/engproc2025089039