Determination of Pesticide Residues in Drinking Water Using the LC-MS/MS Method and Evaluation of the Results for 2023 and 2024 ^†

Abstract

This study focuses on the determination of pesticide residues in drinking water in Slovakia using the LC-MS/MS method, covering a target list of approximately 90 pesticides selected according to the national drinking water risk assessment. The aim of monitoring is to screen the presence of pesticide substances in various water supply systems and to gain experience for setting higher-quality criteria for the control of drinking water. Drinking water samples were collected in 2023, 2024 and 2025 within the National Monitoring Project of the Presence of Pesticides in Public Water Supplies, including both Large-Supply Areas (>5000 inhabitants—2023) and Small-Supply Areas (500–5000 inhabitants—2024, 2025). A total of 211 samples were measured and evaluated in 2023, compared with 199 samples in 2024. This article presents the evaluation of results for 2023 and 2024, while data for 2025 will be assessed in 2026. The findings contribute to the improved surveillance and quality control of drinking water.

1. Introduction

The continuous release of pesticides into the environment and their effects on living organisms make them one of the major concerns in water pollution. Pesticides are significant pollutants in drinking water, posing potential risks to both the environment and human health.

A pesticide is a commercial product placed on the market mainly made up of one or more active substances, along with other ingredients that improve its effectiveness and protect the treated plants. Pesticides are a large group of chemical substances used to kill, repel, or control harmful organisms. They are used to protect crops and plants from pests, diseases, and weeds, helping to increase agricultural productivity and ensure food security. They help reduce crop losses and improve the quality and quantity of food production [1].

The current wording of the legal regulations governing the requirements for drinking water quality in Slovakia does not provide detailed criteria for the control of pesticide substances. The monitoring of pesticides in drinking water is regulated by Act No. 355/2007 Coll. on the Protection, Support, and Development of Public Health [2], and by Decree No. 91/2023 Coll. of the Ministry of Health of the Slovak Republic, which establishes indicators and limit values for drinking water and hot water quality, procedures for drinking water monitoring, risk management of water supply systems, and risk management of domestic distribution systems [3].

According to legislation, only pesticides whose presence can be reasonably expected should be monitored. The limit value for each active substance and their relevant metabolite is set at 0.1 μg/L and 0.5 μg/L for the sum of pesticide residues. Besides the parent active substances, numerous pesticide metabolites occur in the environment as a result of the degradation or chemical transformation of active substances. Pesticide metabolites are classified as relevant—posing a risk comparable to or higher than that of the parent substance, with limit set at 0.1 μg/L—or as irrelevant, in which case they do not represent a significant risk, with limits set between 1 and 6 μg/L [4,5]. The relevance of metabolites is assessed during the approval process of the active substance. A list of pesticides and the relevance of their analyzable metabolites in drinking water is published on the website of the Public Health Authority of the Slovak Republic [6].

Monitoring of pesticide residues in drinking water requires analytical procedures capable of detecting compounds at very low concentrations. Therefore, methods with high sensitivity, accuracy, and reproducibility must be employed. In this study, high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used for the determination of pesticide residues. The materials and analytical procedures are described in the following section.

2. Materials and Methods

2.1. Apparatus and Reagents

The liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was developed at the National Reference Center for Pesticide Residues at the Public Health Authority of the Slovak Republic using a SCIEX Triple Quadrupole 6500+ system (SCIEX, Framingham, MA, USA) equipped with a Turbo V™ ion source and electrospray ionization (ESI), integrated with an ExionLC™ system (SCIEX, Framingham, MA, USA). Data acquisition was performed using Analyst software (SCIEX, Framingham, MA, USA, version 1.7.2). Detection was carried out in both positive- and negative-ion modes with scheduled multiple reaction monitoring (sMRM). The certified standards of all pesticides (each standard was weighed individually and then a mixed solution was prepared, from which working solutions of the required concentrations were made) listed in

Table 1. Determined pesticides divided into groups according to active substance or metabolite: black bold—active substance; red—relevant metabolite; green—irrelevant metabolite; black—metabolite not further specified (evaluation of statistics from calibration solution; partial data from 2025).

Pesticide μg/L (LOD 0.020) (LOQ 0.025)	Active Substance/ Metabolite Relevant or Irrelevant	Limit μg/L	Accreditation	% Blank from Minimal Calibration Point	Correlation Coefficient	QC %	Recovery % Nylon	Recovery % H-PTFE
Acceptation Criteria				<30	>0.995	80–120	80–120	80–120
2,4-D	active substance	0.10 *	N	0.0	0.99700	93	<0	101
2,6-Dichlorobenzamide	irrelevant metabolite	3	N	2.2	0.99981	98	99	101
Acetochlor ESA	relevant metabolite	0.10 *	A	0.0	0.99857	104	N/A	97
Acetochlor OA	relevant metabolite	0.10 *	A	5.2	0.99978	108	56	100
Acetochlor	active substance (forbidden from 2011)	0.10 *	A	0.0	0.99899	104	104	89
Alachlor ESA	irrelevant metabolite	1 **	A	0.0	0.99666	103	N/A	101
Alachlor OA	irrelevant metabolite	1 **	A	8.1	0.99926	99	56	101
Alachlor	active substance (forbidden from 2007)	0.10 *	N	0.0	0.99971	97	83	87
Anthranilic acid isopropylamide		0.10 *	N	0.0	0.99998	104	105	106
Atrazine	active substance (forbidden from 2005)	0.10 *	A	1.6	0.99990	100	100	99
Atrazine-2-hydroxy	irrelevant metabolite	2 **	N	0.5	0.99998	102	103	105
Atrazine-desethyl	relevant metabolite	0.10 *	A	0.0	0.99965	104	103	101
Atrazine-desethyl-desisopropyl	relevant metabolite	0.10 *	N	0.0	0.99758	96	104	100
Atrazine-desisopropyl	relevant metabolite	0.10 *	N	0.0	0.99997	101	103	107
Azoxystrobin (free acid)		0.10 *	N	12.4	0.99927	106	91	96
Azoxystrobin	active substance	0.10 *	N	0.0	0.99961	99	92	91
Bentazone methyl	metabolite		N	0.0	0.99969	100	94	97
Bentazone	active substance	0.10 *	N	0.0	0.99882	101	N/A	108
Carbendazim	metabolite	0.10 *	N	0.0	0.99974	104	102	107
Clopyralid	active substance		N	0.0	0.99902	110	83	119
Cyproconazole	active substance	0.10 *	N	0.0	0.99985	103	100	103
Desmedipham	active substance	0.10 *	N	0.0	0.99939	101	N/A	80
Difenoconazole			N	0.0	0.99975	93	16	88
Diflufenican	active substance	0.10 *	N	0.0	0.99948	95	14	42
Dichlorprop-P	active substance		N	0.0	0.99983	94	<0	98
Dimethachlor ESA	relevant metabolite	0.10 *	A	0.0	0.99844	95	69	105
Dimethachlor OA	relevant metabolite	0.10 *	A	0.0	0.99995	103	97	103
Dimethachlor	active substance	0.10 *	N	0.0	0.99924	97	60	65
Dimethenamid ESA	metabolite		N	0.0	0.99959	101	42	106
Dimethenamid OA	metabolite		N	0.0	0.99999	104	83	105
Dimethenamid-P	active substance			0.0	0.99934	100	98	101
DMS			N	0.0	0.99997	106	106	82
Epoxiconazole	active substance	0.10 *	N	0.0	0.99835	98	94	100
Ethofumesate	active substance	0.10 *	N	0.0	0.99696	99	115	101
Fenpropidin			N	0.0	0.99984	102	93	97
Flufenacet ESA	metabolite		N	0.0	0.99667	101	67	97
Flufenacet OA	metabolite	0.10 *	N	0.0	0.99611	113	84	104
Flufenacet	active substance	0.10 *	N	0.0	0.99986	95	105	99
Fluopicolide			N	0.0	0.99993	95	95	99
Fluroxypyr	active substance	0.10 *	N	0.0	0.99956	105	6	101
Hexazinone			N	0.0	0.99997	101	75	99
Chloridazon	active substance	0.10 *	N	0.0	0.99919	101	99	103
Chloridazon-desphenyl	irrelevant metabolite	6 **	N	0.0	0.99946	96	112	103
Chloridazon-methyl-desphenyl	irrelevant metabolite	6 **	N	0.0	0.99987	108	108	108
Chlormequat chloride	active substance	0.10 *	N	0.0	0.99807	93	90	114
Chlorotoluron	active substance	0.10 *	N	0.0	0.99876	98	80	98
Chlorotoluron-desmethyl		0.10 *	N	0.0	0.99670	96	60	89
Chlorpyrifos	active substance	0.10 *	N	0.0	0.99971	96	10	49
Chlorsulfuron	active substance	0.10 *	N	0.0	0.99896	98	1	96
Imidacloprid			N	0.0	0.99985	92	106	112
Isoproturon	active substance	0.10 *	N	0.0	0.99965	105	98	101
Isoproturon-desmethyl	relevant metabolite	0.10 *	N	0.0	0.99958	94	99	101
Lenacil	active substance	0.10 *	N	0.0	0.99865	117	95	106
Linuron	active substance (forbidden from 2017)	0.10 *	N	0.0	0.99954	97	82	100
MCPA	active substance	0.10 *	N	0.0	0.99976	109	N/A	93
MCPB	active substance	0.10 *	N	0.0	0.99823	97	31	118
Mecoprop	active substance	0.10 *	N	0.0	0.99937	91	<0	95
Mesotrione	active substance	0.10 *	N	0.0	0.99584	106	52	100
Metamitron	active substance	0.10 *	N	0.0	0.99994	100	107	105
Metazachlor ESA	irrelevant metabolite	5 **	N	0.0	0.99937	120	34	103
Metazachlor OA	irrelevant metabolite	5 **	A	0.0	0.99889	108	73	96
Metazachlor	active substance	0.10 *	A	0.0	0.99963	101	104	101
Metolachlor ESA	irrelevant metabolite	6 **	N	0.0	0.99986	103	3	87
Metolachlor OA	irrelevant metabolite	6 **	N	0.0	0.99814	104	55	105
Metribuzin	active substance	0.10 *	N	0.0	0.99937	109	100	102
Metribuzin-desamino-diketo	metabolite/relevant		N	0.0	0.99986	107	125	118
N,N-Dimethyl-2-sulfamoylnicotinamide		0.10 *	N	0.0	0.99913	105	106	96
Nicosulfuron	active substance	0.10 *	N	0.0	0.99995	101	80	108
Parathion-ethyl			N	0.0	0.99735	112	93	80
Pendimethalin	active substance	0.10 *	N	0.0	0.99985	97	6	33
Pethoxamid ESA	metabolite/relevant		N	0.0	0.99922	93	18	88
Pethoxamid	active substance	0.10 *	N	0.0	0.99986	97	98	97
Phenmedipham	active substance	0.10 *	N	0.0	0.99868	99	1	74
Prochloraz	active substance	0.10 *	N	0.0	0.9994	92	91	102
Prometryn	active substance	0.10 *	A	0.0	0.99985	102	102	101
Propazine	active substance	0.10 *	A	0.0	0.99988	99	100	102
Propiconazole	active substance	0.10 *	N	0.0	0.99989	96	96	95
Prosulfocarb			N	0.0	0.99989	96	75	85
Prothioconazole	active substance		N	0.0	0.99714	86	N/A	70
Pyraclostrobin			N	0.0	0.99993	95	1	83
Quinmerac	active substance	0.10 *	N	0.0	0.99977	103	58	98
Simazine	active substance (forbidden from 2025)	0.10 *	A	0.0	0.99990	93	88	104
Simazine-2-hydroxy	metabolite/relevant		N	0.0	0.99995	101	104	105
S-Metolachlor	active substance	0.10 *	N	0.0	0.99833	98	97	100
Tebuconazole	active substance	0.10 *	N	0.0	0.99984	94	94	101
Terbuthylazine	active substance	0.10 *	A	0.0	0.99995	98	98	99
Terbuthylazine-2-hydroxy	relevant metabolite	0.10 *	N	0.0	0.99974	104	106	104
Terbuthylazine-desethyl	relevant metabolite	0.10 *	A	0.0	0.99994	98	92	102
Terbuthylazine-desethyl-2-hydroxy	relevant metabolite	0.10 *	N	0.0	0.99993	103	104	106
Terbutryn	active substance	0.10 *	N	0.0	0.99998	100	97	98
Thiabendazole			N	0.0	0.99999	102	101	107
Thiophanate-methyl		0.10 *	N	0.0	0.99990	100	72	104

Accreditation: A—accredited; N—non-accredited; LOD—limit of detection; LOQ—limit of quantitation; QC—quality control solution. * Decree of the Ministry of Health of the Slovak Republic No. 91/2023 Coll., which details the quality of drinking water, drinking water quality control, the monitoring program, and risk management in drinking water supply [2,3], ** Act No. 355/2007 Coll. on the Protection, Promotion and Development of Public Health and on Amendments and Supplements to Certain Acts, as amended [2,5]. Empty fields indicate that these values have not yet been determined, either in terms of relevance or limit, with the metabolite not further specified.

were purchased from HPC Standards GmbH (accredited by DAkkS, Cramlington, Northumberland, United Kingdom) or LGC Standards Ltd. (accreditated by DAkkS, Teddington, Middlesex, United Kingdom).

Details of the LC and MS/MS conditions are provided below, in

Table 2. Actual optimized LC conditions used in 2025.

LC Conditions
Column:	Kinetex® (Phenomenex, Torrance, CA, USA) 2.6 μm Polar C18 100 Å, 100 × 2.1 mm
Column temperature:	30 °C
Injection volume:	20 μL
Autosampler temperature:	15 °C
Mobile phase A:	0.1% Formic acid in deionized water
Mobile phase B:	Methanol
Run time:	17 min
Flow rate:	0.2 mL/min

,

Table 3. Actual LC method used in 2025, gradient elution.

Time (min)	Mobile Phase A (%)	Mobile Phase B (%)
0	95	5
2	95	5
6	55	45
10	5	95
13	5	95
13.05	0	100
17	0	100

and

Table 4. Actual optimized MS/MS conditions used in 2025.

MS/MS Conditions
Scan type:	Scheduled MRM
Target cycle time:	1 s
Ion source:	ESI
Curtain gas (CUR):	40 psi
Collision gas (CAD):	Medium
Ion Spray Voltage (IS):	5500 V
Temperature (TEM):	400 °C
Ion source Gas 1 (GS1):	50 psi
Ion source Gas 2 (GS2):	50 psi
Entrance Potential (EP):	10 V

.

Methanol (LC-MS grade) used for chromatography was obtained from VWR BDH Chemical (Gdansk, Poland). Formic acid (≥98%, for LC–MS/MS) was obtained from Honeywell Research Chemicals (Fluka™) (Seelze, Germany). Ultra-pure water was produced using a Thermo GenPure Werner purification system (Thermo Fisher Scientific, Waltham, MA, USA).

2.2. Samples and Sample Pretreatment

Drinking water samples were directly filtered, which represented the only sample pretreatment step. Nylon membrane filters with a pore size of 0.22 μm and a diameter of 25 mm (AZ-CF-NYL-25-022, supplied by AZ Chrom s.r.o., Bratislava, Slovakia) and hydrophilic PTFE (H-PTFE) filters with a pore size of 0.22 μm (AZ-CF-HPTFE-25-022, supplied by AZ Chrom s.r.o., Bratislava, Slovakia) were used for filtration.

2.3. Preparation of Blank, Calibration Solutions and Solution for Recovery

Deionized water (Werner) was used as a blank sample, and the samples spiked with the group of pesticides and their metabolites for recovery determination purposes were filtered through both a nylon filter and H-PTFE filters. Recoveries were obtained for concentrations corresponding to the limits at a concentration of 0.1 μg/L. For calibration solutions, the blank sample was spiked with the group of pesticides and their metabolites listed in Table 1 at five levels: 25 ng/L, 50 ng/L, 100 ng/L, 200 ng/L and 400 ng/L. No internal standards were used in this study.

3. Results

3.1. Validation and/or Verification of Method for Pesticide Residues

In Table 1, active substances and their relevant or irrelevant metabolites are presented, together with their limits, accreditation status, blank responses to the lowest calibration level (25 ng/L), correlation coefficients, quality control results, and recoveries obtained using different types of filters.

Most pesticides showed no response in the blank samples; however, a few compounds—2,6-dichlorobenzamide, Acetochlor OA, Alachlor OA, Atrazine, Atrazine-2-hydroxy, and Azoxystrobin (free acid)—exhibited minor blank responses, still below the acceptance criterion of 30%. Correlation coefficients ranged from 0.9958 to 1.000. The quality control (QC) solution at a concentration of 100 ng/L is the same solution as that at the third level of the calibration curve and was measured at the end of each sequence to verify the stability of analytes during measurement. The acceptance criteria of 80–120% were fulfilled for all analytes. Acceptance criteria were adopted from the SANTE/11312/2021 guideline for the determination of pesticide residues in baby food [7].

The recovery solution was prepared in the same manner as the QC and calibration solution at 100 ng/L, with the only difference being that it was filtered through the same filter as the samples were to ensure that no losses of analytes occurred during filtration (a few milliliters was discarded to saturate the filter).

In previous analyses, drinking water samples were pretreated by filtration through nylon filters; however, during the development of our method, it was found that many pesticides were absorbed by nylon filters—up to 100% in some cases. Experimental research identified H-PTFE filters as more suitable, although four pesticides (Diflufenican, Dimetachlor, Chlorpyrifos, and Pendimethalin in Table 1) were still partially absorbed (35–67%). Fortunately, none of these pesticides were detected in the analyzed samples. In the case of future positive findings, calibration solutions will be filtered and compared with the samples to ensure data reliability.

In the future, the National Reference Center for Pesticide Residues will aim to identify a more suitable filter type or another appropriate sample pretreatment method.

3.2. Samples Determination

During the past few years (2023, 2024, 2025), lots of samples from different types of areas were analyzed to ensure appropriate quality control of drinking water according to the National Monitoring Project of the Presence of Pesticides in Drinking Water in Public Water Supplies.

Drinking water samples were collected by regional public health authorities. Sampling sites mainly included areas with agricultural activity, but also sources near golf courses, environmental burdens, areas with previously identified exceedances of pesticide concentrations, and regions with the highest amounts of pesticide use. Priority was given to sampling from facilities such as kindergartens and primary schools, social service homes, municipal offices, shops, etc., while also considering the various types of water sources and disinfection treatments used.

The first phase of the project (2023) focused on supplied areas with the status of LSA (“Large Supply Area”—supplying more than 5000 inhabitants). In 99 LSAs, a total of 211 drinking water samples were collected and 16,088 analytes were examined; pesticides above the limit of detection were found in 171 cases (0.105% of all measured analytes). The results of Phase I are detailed in the paper “Results of Monitoring Pesticide Substances in Drinking Water in Slovakia in 2023” [8].

The second phase of the project (2024) involved continued monitoring in areas with the status of SSA (“Small Supply Area”—supplying 500 to 5000 inhabitants, see Figure 1)—Category IV (500 to 2000 inhabitants) and Category V (2000 to 5000 inhabitants). Analyses of pesticide substances were supplemented with monitoring of disinfection by-products and ecotoxicological tests. In 53 SSAs in Category V and 47 SSAs in Category IV, a total of 199 drinking water samples were collected. In 23.6% of samples, one or more pesticide substances above the limit of detection were identified [9].

The third phase of the project (2025) will continue in the second half of 2025 with the monitoring of pesticides in additional SSAs.

4. Discussion

In 2023, a total of 211 drinking water samples were collected, and pesticides above the limit of detection were found in 171 cases (0.105% of all measured analytes). Screening of drinking water in LSAs in 2023 revealed only seven active substances (Prochloraz, Azoxystrobin, Atrazine, Prometrin, S-metolachlor, Chloridazon, and Bentazon); of these, three active substances were no longer used in plant protection products as of 31 December 2023: Atrazine (authorized in Slovakia until 9 September 2005), Prometrin (authorized until 31 December 2007), and Chloridazon (authorized until 9 June 2022). Atrazine was the most frequently detected active substance (61.9%). Metabolites occurred more frequently than active substances (in up to 87.8% of detections).

Among the metabolites, non-relevant metabolites predominated (71.3% of cases)—most frequently Metolachlor ESA (42% of cases); among the relevant metabolites, Desethyl-desisopropyl-atrazine was most frequently detected (42.9%).

Only 0.012% of all detected results (2 values out of 16,088 determinations) exceeded the limit value of 0.1 µg/L established by the Decree of the Ministry of Health of the Slovak Republic No. 91/2023 Coll. [3]; these were the relevant metabolites Acetochlor ESA and Desethylatrazine.

For Acetochlor ESA, the resulting value for the first drinking water sampling performed on 13 July 2023 was 0.107 µg/L, and for the second control drinking water sampling performed on 27 September 2023, the value was 0.110 µg/L. The limit exceedance was confirmed. The Regional Public Health Authority informed the drinking water supplier, who carried out the sampling on 24 October 2023 and 20 November 2023, with results of 0.089 µg/L and 0.092 µg/L, both being within the acceptable limit. A report was submitted to the State Water Administration Authority, the District Environmental Office in Senica, and an investigation was carried out. The use of products that could have been the source of the metabolite contamination was discontinued.

For Desethylatrazine, the resulting value for the first drinking water sampling performed on 27 November 2023 was 0.227 µg/L, and for the second controlling drinking water sampling performed on 2 January 2024, the value was 0.307 µg/L. The limit exceedance was confirmed. As a result of the investigation, the Regional Public Health Authority informed the drinking water supplier, and on 11 January 2024, a ban on the use of drinking water for drinking, cooking, and food preparation was issued for the water source, which was deemed unsafe according to §12(2)(i) of Act No. 355/2007 Coll. The non-compliant water source was taken out of operation and replaced by an alternative water source.

The monitoring confirmed a favorable situation regarding the occurrence of pesticide substances in LSAs [8,9].

In 2024, a total of 77 pesticide substances were analyzed in 199 samples. At least one pesticide was detected above the limit of detection (LOD) in 47 samples (23.6% of samples). In 38 samples (19.1%), one or more pesticides were detected above the limit of quantitation (LOQ). In 15 samples, three or more pesticides were detected, and in another 3 samples, five pesticides were detected.

The highest detection rates in 2024 were recorded in the Trnava region (51.1%), followed by Bratislava (36.4%), Trenčín (35.7%), and Nitra (31.3%) (see Figure 2).

The most frequently detected substances in 2024 were Chloridazon metabolites (Chloridazon-desphenyl in 12.6% and Chloridazon-methyl-desphenyl in 6.0% of samples). The active substance Chloridazon was found in only one sample. Atrazine, the most commonly detected active ingredient, was found in 5.0% of samples. Although these substances are no longer approved in the EU, their presence is likely due to their persistence in the environment.

In none of the analyzed samples was the parametric value (0.1 µg/L for individual pesticides or 0.5 µg/L for the sum of pesticides) exceeded. The highest concentrations were observed for Chloridazon-desphenyl, reaching 1.31 µg/L; however, as an irrelevant metabolite according to the decision of the Chief Hygienist of the Slovak Republic (No. OHŽP/430/89726/2019), it has a maximum limit of 6 µg/L [5]. The compounds closest to the parametric value were Atrazine-desethyl (0.098 µg/L), Chlorsulfuron (0.077 µg/L), and Atrazine (0.074 µg/L) [9].

5. Conclusions

This validated LC-MS/MS method provides a powerful tool for the routine, high-throughput monitoring of pesticide residues in drinking water. Its high sensitivity and selectivity allow for the reliable detection of trace levels of pesticides and their metabolites, meeting current regulatory requirements.

In 2023, screening of drinking water in LSAs revealed seven active substances (Prochloraz, Azoxystrobin, Atrazine, Prometrin, S-metolachlor, Chloridazon, and Bentazon). Atrazine was the most frequently detected active substance (61.9%). Metabolites occurred more frequently than active substances (in up to 87.8% of detections).

Among the metabolites, non-relevant metabolites predominated (71.3% of cases)—most frequently Metolachlor ESA (42% of cases); among the relevant metabolites, Desethyl-desisopropyl-atrazine was most frequently detected (42.9%). Only two of all detected results exceeded the limit value of 0.1 µg/L; these were the relevant metabolites Acetochlor ESA and Desethylatrazine [8].

Importantly, no sample exceeded the legal limits for drinking water in 2024. The highest concentrations were observed for Chloridazon-desphenyl, reaching 1.31 µg/L; however, it has a maximum limit of 6 µg/L [5]. The compounds with values closest to the parametric value were Atrazine-desethyl (0.098 µg/L), Chlorsulfuron (0.077 µg/L), and Atrazine (0.074 µg/L) [9].

The results for 2025 will be evaluated in 2026 and presented in the next publication. For now, it cannot be stated that the decreasing occurrence of active substances and their metabolites observed in 2023 and 2024 will continue as a trend, due to the differences in water supply systems, even if they are located in the same geographical region.