Enzymatic Activity as a Measure of Total Microbial Activity on Historical Stone
Abstract
:1. Introduction
2. Materials and Methods
2.1. Description of the Studied Sites
2.2. Sampling
2.3. Determination of Biological Activity
2.3.1. Fluorescein Diacetate (FDA) Method
2.3.2. Plate Count Method
- N = (C/3) × D × V
- N—CFU per gram or mL
- C—Total colony count of 3 petri dishes
- D—Dilution factor
- V—Transferred volume (mL)
3. Results
3.1. Spectrophotometric Measurements
3.2. Plate Count Method
3.3. Enzymatic Activity/Total Microbial Activity and Total Number of Bacteria
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Code | Absorbance (490 nm) | Concentration (µg Fluorescein/mL) | Enzymatic Activity (µg Fluorescein/g) |
---|---|---|---|
S1 | 0.076 | 0.38 | 3.14 |
S2 | 0.125 | 0.63 | 5.17 |
S3 | 0.089 | 0.45 | 7.36 |
L1 | 0.074 | 0.37 | 6.12 |
L2 | 0.157 | 0.79 | 6.49 |
L3 | 0.157 | 0.79 | 6.49 |
L4 | 0.105 | 0.53 | 4.34 |
L5 | 0.214 | 1.07 | 9.32 |
Sample Code | Absorbance (490 nm) | Concentration (µg Fluorescein/mL) | Enzymatic Activity (µg Fluorescein/g) |
---|---|---|---|
S4 | 0.661 | 3.31 | 54.67 |
S5 | 1.210 | 6.06 | 50.02 |
S6 | 0.755 | 3.78 | 31.22 |
S7 | 0.175 | 0.88 | 260 |
L6 | 1.519 | 7.60 | 1134.90 |
L7 | 1.295 | 6.48 | 899.46 |
L8 | 0.356 | 1.79 | 708.75 |
L9 | 1.044 | 5.22 | 721.83 |
L10 | 0.497 | 2.49 | 20.55 |
Samples without Lichens | Samples with Lichens | ||
---|---|---|---|
Sample Code | Total Number of Bacteria (×104 CFU/g of Stone) | Sample Code | Total Number of Bacteria (×104 CFU/g of Stone) |
S1 | 1.40 | S4 | 0.6 |
S2 | 2.40 | S5 | 11.0 |
S3 | 2.60 | S6 | 13.5 |
L1 | 3.90 | S7 | 7.24 |
L2 | 1.20 | L6 | 20.57 |
L3 | 4.70 | L7 | 19.9 |
L4 | 6.90 | L8 | 22.2 |
L5 | 8.02 | L9 | 10.3 |
L10 | 11.3 |
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Sırt Çıplak, E.; Akoğlu, K.G. Enzymatic Activity as a Measure of Total Microbial Activity on Historical Stone. Heritage 2020, 3, 671-681. https://doi.org/10.3390/heritage3030038
Sırt Çıplak E, Akoğlu KG. Enzymatic Activity as a Measure of Total Microbial Activity on Historical Stone. Heritage. 2020; 3(3):671-681. https://doi.org/10.3390/heritage3030038
Chicago/Turabian StyleSırt Çıplak, Elif, and Kiraz Göze Akoğlu. 2020. "Enzymatic Activity as a Measure of Total Microbial Activity on Historical Stone" Heritage 3, no. 3: 671-681. https://doi.org/10.3390/heritage3030038