Backgoround: The genus
Pseudomonas encompasses metabolically versatile bacteria widely distributed in diverse environments, including clinical settings. Among these,
Pseudomonas kurunegalensis is a recently described environmental species with limited clinical characterization.
Objective and Methods: In this study, we report the genomic and phenotypic characterization
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Backgoround: The genus
Pseudomonas encompasses metabolically versatile bacteria widely distributed in diverse environments, including clinical settings. Among these,
Pseudomonas kurunegalensis is a recently described environmental species with limited clinical characterization.
Objective and Methods: In this study, we report the genomic and phenotypic characterization of a
P. kurunegalensis isolate, Pam1317368, recovered from a catheterized urine sample of a post-renal transplant patient without symptoms of urinary tract infection. Initial identification by MALDI-TOF MS misclassified the isolate as
Pseudomonas monteilii. Whole-genome sequencing and average nucleotide identity (ANI) analysis (≥95%) confirmed its identity as
P. kurunegalensis. The methodology included genomic DNA extraction, Illumina sequencing, genome assembly, ANI calculation, antimicrobial susceptibility testing, resistance gene identification and phylogenetic analysis.
Results: Antimicrobial susceptibility testing revealed multidrug resistance, including carbapenem resistance mediated by the metallo-β-lactamase gene
VIM-2. Additional resistance determinants included genes conferring resistance to fluoroquinolones and aminoglycosides. Phylogenetic analysis placed the isolate within the
P. kurunegalensis clade, closely related to environmental strains.
Conclusions: Although the clinical significance of this finding remains unclear, the presence of clinically relevant resistance genes in an environmental
Pseudomonas species isolated from a human sample highlights the value of genomic surveillance and accurate species-level identification in clinical microbiology.
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