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Proceedings 2017, 1(4), 527;

A Miniaturized SPLITT System for On-Line Protein Separation

Fondazione Bruno Kessler, Trento, Italy
Università di Trento, Trento, Italy
Presented at the Eurosensors 2017 Conference, Paris, France, 3–6 September 2017.
Author to whom correspondence should be addressed.
Published: 25 August 2017
(This article belongs to the Proceedings of Eurosensors 2017)
PDF [485 KB, uploaded 26 August 2017]


The development of a microfluidic module for on-line protein separation is presented in this paper. The device is based on the SPLITT (Split flow Thin fractionation) technique and the separation of proteins is electrically driven by means of two platinum electrodes included in the structure of the microdevice. The microfluidic network is realized by means of a thin dry film structure, Ordyl SY 355, (thermo-compression lamination), laminated on three levels, which is patterned through a photolithographic technique. The device has been tested with a Bovine Serum Albumin (BSA) solution, through absorbance measurements with a spectrophotometer, with best achieved separation at the outlet of 40%, measured as relative concentration unbalance at output channels.
Keywords: sample preparation; protein separation; microfluidics; dry film; SPLITT; electrophoresis sample preparation; protein separation; microfluidics; dry film; SPLITT; electrophoresis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Capuano, A.; Adami, A.; Mulloni, V.; Lorenzelli, L. A Miniaturized SPLITT System for On-Line Protein Separation. Proceedings 2017, 1, 527.

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