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Proceedings 2017, 1(10), 1022;

The Role of Trophoblastic Stem Cells Conditioned Media on JAR Cell Culture

Department of Histology and Embryology, School of Medicine, Manisa Celal Bayar University, Manisa 45000, Turkey
Experimental Health Science Research Center, Near East University, Nicosia 99138, Cyprus
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017.
Author to whom correspondence should be addressed.
Published: 10 November 2017
PDF [135 KB, uploaded 13 November 2017]


Trophoblasts are placenta-specific epithelial cells which play an important role in embryo implantation. Trophoblast stem cells (TSCs) are precursor cells of placenta and can be derived before or after implantation. During TSCs culture, several molecules and small vesicles such as exosome are secreted. Exosomes, play an important role in intercellular communication, signal transduction and cell property regulation via carrying genetic material. The aim of the study was to investigate the role of TSCs released factors on human choriocarcinoma cell line (JAR). TSCs were obtained from E14 placenta and cultured for a week. Then conditioned media were collected. JARs were cultured in JAR culture media with/without TSCs conditioned media for 48h then anti-MMP2, anti-Jagged1, anti-IL10 immunoreactivities were analyzed. The culture media were also collected for evaluation of Exosome and miRNA using miRCURY™ Kit. After TSCs conditioned media incubation; JAR cells spheroids size was different and signaling molecules expression for invasion and adhesion decreased. The amount of miRNA was changed after incubation. In conclusion, TSCs conditioned media was effect JAR cells properties via expressing of signaling molecules and exosomal-fragments. Therefore, TSCs products, which might be used to reduce different cancer cells invasion and adhesion properties, could be further analyzed.
Keywords: TSCs; JAR; miRNA TSCs; JAR; miRNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Kabadayı, H.; Kendirci, R.; Vatansever, H.S. The Role of Trophoblastic Stem Cells Conditioned Media on JAR Cell Culture. Proceedings 2017, 1, 1022.

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