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Article

An Electrospinning Sample Delivery Device for Synchrotron-Based Biomacromolecule Serial Crystallography Research

by
Li Yu
1,2,†,
Zhijun Wang
3,†,
Qin Xu
3,†,
Bo Sun
1,2,3,*,
Qingjie Xiao
3,
Weiwei Wang
3,
Yuzhu Wang
3,
Qisheng Wang
1,2,3 and
Jianhua He
4,*
1
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
3
Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, China
4
The Institute for Advanced Studies, Wuhan University, Wuhan 430072, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Quantum Beam Sci. 2025, 9(2), 17; https://doi.org/10.3390/qubs9020017
Submission received: 28 February 2025 / Revised: 3 April 2025 / Accepted: 17 April 2025 / Published: 5 May 2025
(This article belongs to the Section Instrumentation and Facilities)
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Abstract

Serial crystallography is a rapidly advancing experimental technology that has seen significant development in recent years. This technique enables the continuous delivery of a series of protein crystal samples to the X-ray beam, allowing for the collection of diffraction data from a large number of crystals at ambient temperature. Despite its advancements, serial crystallography still possesses considerable potential for further development within synchrotron radiation platforms. Currently, several challenges hinder the progress of this technology, including the preparation of numerous microcrystal samples, methods for sample delivery, data acquisition efficiency, and data processing techniques. The device introduced in this paper is designed to facilitate serial crystallographic experiments at the synchrotron radiation station, employing electrospinning in the vacuum cavity to reduce the average flux, mitigate the effects of air ionization on the Taylor cone, and enhance the stability of Taylor cone during the data acquisition process. The diffraction pattern of lysozyme crystals was successfully acquired with this device at the beamlines of the Shanghai Synchrotron Radiation Facility (SSRF).
Keywords: serial crystallography; electrospinning; diffraction before destroy; synchrotron radiation serial crystallography; electrospinning; diffraction before destroy; synchrotron radiation

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MDPI and ACS Style

Yu, L.; Wang, Z.; Xu, Q.; Sun, B.; Xiao, Q.; Wang, W.; Wang, Y.; Wang, Q.; He, J. An Electrospinning Sample Delivery Device for Synchrotron-Based Biomacromolecule Serial Crystallography Research. Quantum Beam Sci. 2025, 9, 17. https://doi.org/10.3390/qubs9020017

AMA Style

Yu L, Wang Z, Xu Q, Sun B, Xiao Q, Wang W, Wang Y, Wang Q, He J. An Electrospinning Sample Delivery Device for Synchrotron-Based Biomacromolecule Serial Crystallography Research. Quantum Beam Science. 2025; 9(2):17. https://doi.org/10.3390/qubs9020017

Chicago/Turabian Style

Yu, Li, Zhijun Wang, Qin Xu, Bo Sun, Qingjie Xiao, Weiwei Wang, Yuzhu Wang, Qisheng Wang, and Jianhua He. 2025. "An Electrospinning Sample Delivery Device for Synchrotron-Based Biomacromolecule Serial Crystallography Research" Quantum Beam Science 9, no. 2: 17. https://doi.org/10.3390/qubs9020017

APA Style

Yu, L., Wang, Z., Xu, Q., Sun, B., Xiao, Q., Wang, W., Wang, Y., Wang, Q., & He, J. (2025). An Electrospinning Sample Delivery Device for Synchrotron-Based Biomacromolecule Serial Crystallography Research. Quantum Beam Science, 9(2), 17. https://doi.org/10.3390/qubs9020017

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