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Open AccessArticle

Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform

1
BluSense Diagnostics, Fruebjergvej 3, DK-2100 København, Denmark
2
Advanced Processing Technology Research Center (APT), School of Mechanical Engineering, Dublin City University, 9 Dublin, Ireland
3
Water Institute, Dublin City University, 9 Dublin, Ireland
4
Biomedical Diagnostics Institute, Dublin City University, 9 Dublin, Ireland
5
Biosurfit SA, Rua 25 de Abril nº66, 2050-317 Azambuja, Portugal
6
Fraunhofer Project Center at Dublin City University ([email protected]), School of Physical Sciences, Dublin City University, 9 Dublin, Ireland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Inventions 2020, 5(1), 1; https://doi.org/10.3390/inventions5010001
Received: 2 December 2019 / Accepted: 18 December 2019 / Published: 22 December 2019
(This article belongs to the Special Issue Microfluidic Devices)
We present a powerful and compact batch-mode mixing and dilution technique for centrifugal microfluidic platforms. Siphon structures are designed to discretize continuous flows into a sequence of droplets of volumes as low as 100 nL. Using a passive, self-regulating 4-step mechanism, discrete volumes of two fluids are alternatingly issued into a common intermediate chamber. At its base, a capillary valve acts as a fluidic shift register; a single droplet is held in place while two or more droplets merge and pass through the capillary stop. These merged droplets are advectively mixed as they pass through the capillary valve and into the receiving chamber. Mixing is demonstrated for various combinations of liquids such as aqueous solutions as well as saline solutions and human plasma. The mixing quality is assessed on a quantitative scale by using a colorimetric method based on the mixing of potassium thiocyanate and iron(III) chloride, and in the case of human plasma using a spectroscopic method. For instance, volumes of 5 µL have been mixed in less than 20 s. Single-step dilutions up to 1:5 of plasma in a standard phosphate buffer solution are also demonstrated. This work describes the preliminary development of the mixing method which has since been integrated into a commercially available microfluidic cartridge. View Full-Text
Keywords: Lab-on-a-Chip; centrifugal microfluidics; Lab-on-a-Disc; mixing; siphon valves Lab-on-a-Chip; centrifugal microfluidics; Lab-on-a-Disc; mixing; siphon valves
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MDPI and ACS Style

Burger, R.; Kinahan, D.J; Cayron, H.; Reis, N.; Fonseca, J.; Ducrée, J. Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform. Inventions 2020, 5, 1. https://doi.org/10.3390/inventions5010001

AMA Style

Burger R, Kinahan DJ, Cayron H, Reis N, Fonseca J, Ducrée J. Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform. Inventions. 2020; 5(1):1. https://doi.org/10.3390/inventions5010001

Chicago/Turabian Style

Burger, Robert; Kinahan, David J; Cayron, Hélène; Reis, Nuno; Fonseca, João; Ducrée, Jens. 2020. "Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform" Inventions 5, no. 1: 1. https://doi.org/10.3390/inventions5010001

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