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A Simple ImageJ-Based Method to Measure Cardiac Rhythm in Zebrafish Embryos

Department of Bioscience Technology, Chung Yuan Christian University, 200 Chung-Pei Rd., Chung-Li 32023, Taiwan
Department of Chemistry, Chinese Culture University, Taipei 11114, Taiwan
Center of Nanotechnology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
Center of Biomedical Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
Authors to whom correspondence should be addressed.
Inventions 2018, 3(2), 21;
Received: 18 March 2018 / Revised: 1 April 2018 / Accepted: 3 April 2018 / Published: 10 April 2018
(This article belongs to the Section Inventions and innovation in Biotechnology and Materials)
PDF [15781 KB, uploaded 3 May 2018]


Zebrafish has an advantage of optical clarity within the body during its early developmental stage. Therefore, organs, including the heart, can be observed and analyzed in a non-invasive manner. Previously, heart rate measurement was analyzed by a complicated system, programming language, fluorescence transgenic zebrafish, or expensive setup that was not feasible and practical. In this study, we established a simple dissecting microscope with Charge-coupled Device (CCD) to capture the signal of heartbeat, and used ImageJ software as an open source platform to analyze cardiac rhythm for the atrium and the ventricle. We subjected astemizole to zebrafish embryos, and applied our apparatus to precisely detect heart arrhythmia (Atrium–Ventricle block) induction. In other words, we provided a simple, economical, and reliable method to measure cardiac rhythm in zebrafish embryos, which is able to evaluate whether cardiac rhythm is affected by chemical compounds. View Full-Text
Keywords: ImageJ; region of interest (ROI); cardiac rhythm; arrhythmia; zebrafish ImageJ; region of interest (ROI); cardiac rhythm; arrhythmia; zebrafish

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Sampurna, B.P.; Audira, G.; Juniardi, S.; Lai, Y.-H.; Hsiao, C.-D. A Simple ImageJ-Based Method to Measure Cardiac Rhythm in Zebrafish Embryos. Inventions 2018, 3, 21.

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