Use of Light Spectra for Efficient Production of PLBs in Temperate Terrestrial Orchids
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Explant Preparation
2.2. Media and Culture Conditions
2.3. Germination and Acclimatization
2.4. Statistical Analysis
3. Results
3.1. Effects of PGRs, Explant Type, and Wounding on DSE
3.2. Induction of SE under Different Light Spectra
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Plant | Concentration of PGRs (mg/L) | Explant Type | PLBs Formation (%) | PLBs Growth (%) | ||||||
---|---|---|---|---|---|---|---|---|---|---|
TDZ | NAA | BA | Kin | Protocorm | Leaf | Node | Crown | |||
tiEpipactis verafolia | 0 | 0 | 0 | 0 | × | - | - | - | 0 | 0 |
3 | 0 | 0 | 0 | × | - | - | - | 100 a | 100 a | |
0 | 0 | 1 | 0 | - | × | - | - | 0 | 0 | |
0 | 0 | 1 | 0 | - | - | × | - | 0 | 0 | |
0 | 0 | 2 | 0 | - | × | - | - | 0 | 0 | |
0 | 0 | 2 | 0 | - | - | × | - | 0 | 0 | |
0 | 0 | 3 | 0 | - | × | - | - | 0 | 0 | |
0 | 0 | 3 | 0 | - | - | × | - | 0 | 0 | |
0 | 0.5 | 0 | 0 | - | × | - | - | 0 | 0 | |
0 | 0.5 | 0 | 0 | - | - | × | - | 25 b | 25 d | |
0 | 0.5 | 1 | 0 | - | × | - | - | 0 | 0 | |
0 | 0.5 | 1 | 0 | - | - | × | - | 25 b | 75 b | |
0 | 0.5 | 2 | 0 | - | × | - | - | 0 | 0 | |
0 | 0.5 | 2 | 0 | - | - | × | - | 0 | 0 | |
0 | 0.5 | 3 | 0 | - | × | - | - | 0 | 0 | |
0 | 0.5 | 3 | 0 | - | - | × | - | 0 | 0 | |
0 | 1 | 0 | 0 | - | × | - | - | 0 | 0 | |
0 | 1 | 0 | 0 | - | - | × | - | 38 a | 20 d | |
0 | 1 | 1 | 0 | - | × | - | - | 0 | 0 | |
0 | 1 | 1 | 0 | - | - | × | - | 25 b | 50 c | |
0 | 1 | 2 | 0 | - | × | - | - | 0 | 0 | |
0 | 1 | 2 | 0 | - | - | × | - | 18 c | 75 b | |
0 | 1 | 3 | 0 | - | × | - | - | 0 | 0 | |
0 | 1 | 3 | 0 | - | - | × | - | 0 | 0 | |
0 | 0 | 1 | 0 | - | × | - | - | 0 | 0 | |
0 | 0 | 1 | 0 | - | - | × | - | 0 | 0 | |
0 | 0 | 2 | 0 | - | × | - | - | 0 | 0 | |
0 | 0 | 2 | 0 | - | - | × | - | 25 b | 50 c | |
0 | 0 | 3 | 0 | - | × | - | - | 0 | 0 | |
0 | 0 | 3 | 0 | - | - | × | - | 13 c | 100 a | |
Dactylorhiza umberosa | 0 | 0 | 0 | 0 | × | - | - | - | 0 | 0 |
3 | 0 | 0 | 0 | × | - | - | - | 100 a | 90 b | |
0 | 0 | 0 | 1 | × | - | - | - | 0 | 0 | |
0 | 0 | 0 | 1.5 | × | - | - | - | 25 c | 75 b | |
0 | 0 | 0 | 2 | × | - | - | - | 50 b | 100 a | |
2 | 0 | 2 | 0 | - | - | - | × | 0 | 0 | |
1 | 0.5 | 0 | 0 | - | - | - | × | 0 | 0 | |
0 | 0.5 | 2 | 0 | - | - | - | × | 0 | 0 |
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Naderi Boldaji, H.; Dianati Daylami, S.; Vahdati, K. Use of Light Spectra for Efficient Production of PLBs in Temperate Terrestrial Orchids. Horticulturae 2023, 9, 1007. https://doi.org/10.3390/horticulturae9091007
Naderi Boldaji H, Dianati Daylami S, Vahdati K. Use of Light Spectra for Efficient Production of PLBs in Temperate Terrestrial Orchids. Horticulturae. 2023; 9(9):1007. https://doi.org/10.3390/horticulturae9091007
Chicago/Turabian StyleNaderi Boldaji, Hossein, Shirin Dianati Daylami, and Kourosh Vahdati. 2023. "Use of Light Spectra for Efficient Production of PLBs in Temperate Terrestrial Orchids" Horticulturae 9, no. 9: 1007. https://doi.org/10.3390/horticulturae9091007