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Article

In Vitro Culture of Eremurus spectabilis (Liliaceae), a Rare Ornamental and Medicinal Plant, through Root Explants

1
Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
2
Department of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan 4918943464, Iran
3
Research Center for Healthcare Industry Innovation, National Taipei University of Nursing and Health Sciences, Taipei 112, Taiwan
4
School of Nursing, National Taipei University of Nursing and Health Sciences, Taipei 112, Taiwan
*
Authors to whom correspondence should be addressed.
Academic Editors: Jean Carlos Bettoni, Min-Rui Wang, Qiao-Chun Wang and Barbara Ruffoni
Horticulturae 2022, 8(3), 202; https://doi.org/10.3390/horticulturae8030202
Received: 23 January 2022 / Revised: 13 February 2022 / Accepted: 22 February 2022 / Published: 25 February 2022
Eremurus spectabilis M. Bieb, a perennial herbaceous wild species, is commonly used in the horticultural, ornamental, and pharmaceutical markets. Studies on the tissue culture systems for this species would be beneficial for mass multiplication as well as for future breeding programs. An in vitro propagation technique was established here using tuberous root explants as unique and responsive starting materials for culture initiation. The proliferated calli were sub-cultured on shoot proliferation media and regenerated microshoots were assessed. The shoot proliferation rate, leaf number, leaf length, and chlorophyll and carotenoid contents were recorded. The highest callus induction per explant (76.67%), callus dry weight (10.25 mg), callus firmness ratio (3.97), and callus color intensity ratio (2.83) were observed in explants inoculated on Murashige and Skoog (MS) medium supplemented with 10.0 mgL−1 6-benzylaminopurine (BAP). The highest shoot proliferation rates were obtained when calli were sub-cultured on MS or Schenk and Hildebrandt (SH) basal media supplemented with 2.0 mgL−1 BAP. The half-strength MS medium fortified with 4.0% sucrose + 2.0 mgL−1 indole butyric acid (IBA) + 200 mgL−1 activated charcoal was a superior combination for root emergence and rooting parameters. Regenerated plantlets were then successfully adapted to ex vitro conditions. The reported protocol can be exploited at a commercial scale following minor modification, or could be beneficial in the production of secondary metabolites in bioreactors where callus is required as fresh plant material. View Full-Text
Keywords: callus induction; foxtail lily; micropropagation; proliferation rate; acclimatization callus induction; foxtail lily; micropropagation; proliferation rate; acclimatization
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MDPI and ACS Style

Basiri, Y.; Etemadi, N.; Alizadeh, M.; Alizargar, J. In Vitro Culture of Eremurus spectabilis (Liliaceae), a Rare Ornamental and Medicinal Plant, through Root Explants. Horticulturae 2022, 8, 202. https://doi.org/10.3390/horticulturae8030202

AMA Style

Basiri Y, Etemadi N, Alizadeh M, Alizargar J. In Vitro Culture of Eremurus spectabilis (Liliaceae), a Rare Ornamental and Medicinal Plant, through Root Explants. Horticulturae. 2022; 8(3):202. https://doi.org/10.3390/horticulturae8030202

Chicago/Turabian Style

Basiri, Yeganeh, Nematollah Etemadi, Mahdi Alizadeh, and Javad Alizargar. 2022. "In Vitro Culture of Eremurus spectabilis (Liliaceae), a Rare Ornamental and Medicinal Plant, through Root Explants" Horticulturae 8, no. 3: 202. https://doi.org/10.3390/horticulturae8030202

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