Genetic Diversity of Tea Plant (Camellia sinensis (L.) Kuntze) Germplasm Resources in Wuyi Mountain of China Based on Single Nucleotide Polymorphism (SNP) Markers
Round 1
Reviewer 1 Report
This manuscript has some edit in the author name of botanical name, location of this research area, some reference in text.
Comments for author File: 
Comments.pdf
Author Response
尊敬的审稿人：
感谢您对我们题为“基于单核苷酸多态性（SNP）标记的武夷山茶树（ Camellia sinensis ）种质资源遗传多样性”的稿件的评论。您的所有评论都很有价值，对修改和改进我们的手稿非常有帮助，我们也很感激您的评论给了我们很多灵感和对我们未来研究的不同想法。我们仔细研究了评论并进行了更正。因此，我们已经上传了修订稿，并使用 MS Word 中的跟踪更改模式突出显示了所有更改。请在下面查看我们的逐点回复。
Author Response File: 
Author Response.docx
Reviewer 2 Report
This manuscript by Liu et al., describes the genetic diversity of tea plant germplasm resources in Wuyi mountain based on SNP marker. This manuscript is well written, however more information are lacking specially in the materials and method section. Author should describe about how they prepared samples for DNA extraction, how much leaf amount they used, chemicals with amounts should provide along with the DNA extraction kit name. Also, author should provide the primers informations, length, PCR conditions and DNA sequence analysis procedure. I did not found any informations; how performed AMOVA analysis, how they calculate Fst value, how they construct hierarchical clustering tree?
In the results section, authors should provide the major polymorphic sites showing how they are different from each others in one table in manuscript and should provide complete informations of all polymorphic sites along with genbank accession numbers in supplementary file.
Minor suggestions:
Line 82-85: Write the full name with abbreviations while they mention first time and use abbreviations later.
Line 84-85: Remove
Line 91-92: ....Which requires a DNA concentration of ......ratio of 1.7-1.9, remove it.
Table 1. List of 137 tea samples or tea germplasms (WY1-137)? because the meaning of samples and germplasm is totally different.
Please, reformat the table, so it will be easy to readers to differentiate the samples.
Mention the full form of abbreviations from table in the footnote.
Line 267-269: How this results suggest that genetic distance and genetic differentiations among the samples increase with the increasing the geographical distance? Please describe this results very carefully.
Figure 5, Please indicate the samples locations in the figure by using different sign, so it will be more easy to see the genetic and geographic variation.
Author Response
Dear Reviewer:
Thank you for your comments concerning our manuscript entitled “Genetic diversity of tea plant (Camellia sinensis) germplasm resources in Wuyi Mountain based on single nucleotide polymorphism (SNP) markers”. All of your comments are valuable and very helpful for revising and improving our manuscript, and we are also appreciated that your comments have given us a lot of inspiration and different ideas for our future research. We have studied comments carefully and have made corrections. Accordingly, we have uploaded the revised manuscript with all the changes highlighted by using the track changes mode in MS Word. Please see our point-by-point responses below. Please see the attachment.
Author Response File: Author Response.docx
Reviewer 3 Report
Please check the manuscript for comments to make minor changes.
Comments for author File: Comments.pdf
Author Response
Dear Reviewer:
Thank you for your comments concerning our manuscript entitled “Genetic diversity of tea plant (Camellia sinensis) germplasm resources in Wuyi Mountain based on single nucleotide polymorphism (SNP) markers”. All of your comments are valuable and very helpful for revising and improving our manuscript, and we are also appreciated that your comments have given us a lot of inspiration and different ideas for our future research. We have studied comments carefully and have made corrections. Accordingly, we have uploaded the revised manuscript with all the changes highlighted by using the track changes mode in MS Word. Please see our point-by-point responses below. Please see the attachment.
Author Response File: Author Response.docx
Round 2
Reviewer 2 Report
I found substantial improvement in the revised manuscript.
Please follow my minor suggestions as follows:
1. Mention the version of all the analysis softwares. like Mega 6, 7, 10 , or what?
