Mechanism of the Alternaria alternata Pathogenicity in ‘Fortune’ Mandarin
Abstract
:1. Introduction
2. Materials and Methods
2.1. Selection of Alternaria alternata Isolates, Culture of the Fungus, and Pathogenicity Studies
2.2. Extraction and Measurement of A. alternata Mycotoxins
2.3. Extraction and Measurement of Laccase Activity in the Mycelium of A. alternata
2.4. Histological Localization of the Laccase Acitvity
2.5. Statistical Analysis
3. Results
3.1. Study of the Pathogenicity and the Mycotoxins Content of the A. alternata Isolates
3.2. Correlation between the Laccase Activity of A. alternata Isolates and Their Pathogenicity
3.3. Location of Laccase Activity in the Tissues of ‘Fortune’ Fruits Inoculated with A. alternata
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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A. alternata Isolate | Mycotoxin (µmol/g DW) | Laccase Activity (mU/mL) | |
---|---|---|---|
AME | AOH | ||
Alt3 | 32 ± 2 b | 3 ± 0.2 b | 105 ± 21 b |
Alt14 | 58 ± 3 a | 28 ± 4 a | 215 ± 25 a |
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Díaz, L.; Del Río, J.A.; Ortuño, A. Mechanism of the Alternaria alternata Pathogenicity in ‘Fortune’ Mandarin. Horticulturae 2018, 4, 54. https://doi.org/10.3390/horticulturae4040054
Díaz L, Del Río JA, Ortuño A. Mechanism of the Alternaria alternata Pathogenicity in ‘Fortune’ Mandarin. Horticulturae. 2018; 4(4):54. https://doi.org/10.3390/horticulturae4040054
Chicago/Turabian StyleDíaz, Licinio, José Antonio Del Río, and Ana Ortuño. 2018. "Mechanism of the Alternaria alternata Pathogenicity in ‘Fortune’ Mandarin" Horticulturae 4, no. 4: 54. https://doi.org/10.3390/horticulturae4040054
APA StyleDíaz, L., Del Río, J. A., & Ortuño, A. (2018). Mechanism of the Alternaria alternata Pathogenicity in ‘Fortune’ Mandarin. Horticulturae, 4(4), 54. https://doi.org/10.3390/horticulturae4040054