Characterization of Walls Are Thin 1 Family in Cucumis sativus and Functional Identification of CsWAT1-20 in Response to Podosphaera xanthii
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials, Fungal Inoculation, and Growth Conditions
2.2. Identification and Characterization of WAT1s in Cucumber
2.3. Analysis of Structure and Motif and Phylogenetic of CsWAT1s
2.4. Chromosome Localization and Collinearity Analysis of CsWAT1s
2.5. Cis-Acting Elements Analysis of CsWAT1s Promoter
2.6. RT-qPCR Validation
2.7. Virus-Induced Gene Silence Test
2.8. Disease Index Investigation and P. xanthii Mycelia Staining
3. Results
3.1. Identification and Phylogeny Analysis of WAT1s in Cucumis sativus
3.2. Chromosome Localization and Collinearity Analysis of CsWAT1s
3.3. Gene Structure and Motif Distribution of CsWAT1s
3.4. Cis-Acting Elements Analysis of CsWAT1s Promoters
3.5. Expression Profiles of CsWAT1s in Response to P. xanthii Stress
3.6. Silencing of CsWAT1-20 in Cucumber Seedlings Contributes to P. xanthii Stress Tolerance
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gene ID | Rename | Number of Amino Acids | Molecular Weight (kDa) | Theoretical pI | Gravy | Instability Index | Single Peptide |
---|---|---|---|---|---|---|---|
CsaV3_1G004090 | CsWAT1-1 | 362 | 40,240.51 | 7.58 | 0.559 | 36.93 | No |
CsaV3_1G015750 | CsWAT1-2 | 367 | 40,023.02 | 9.34 | 0.402 | 33.84 | No |
CsaV3_1G029400 | CsWAT1-3 | 369 | 40,737.24 | 8.86 | 0.615 | 42.95 | No |
CsaV3_1G037450 | CsWAT1-4 | 354 | 39,043.52 | 9.32 | 0.670 | 32.07 | No |
CsaV3_2G007260 | CsWAT1-5 | 584 | 65,291.84 | 8.84 | 0.451 | 31.17 | No |
CsaV3_2G007860 | CsWAT1-6 | 369 | 40,123.75 | 8.21 | 0.791 | 42.45 | No |
CsaV3_2G011920 | CsWAT1-7 | 394 | 43,412.44 | 9.20 | 0.561 | 42.31 | No |
CsaV3_2G011970 | CsWAT1-8 | 386 | 43,122.36 | 9.40 | 0.314 | 33.06 | No |
CsaV3_2G012900 | CsWAT1-9 | 360 | 40,729.15 | 9.16 | 0.544 | 39.5 | No |
CsaV3_2G013970 | CsWAT1-10 | 386 | 42,020.27 | 9.58 | 0.742 | 33.34 | No |
CsaV3_2G018030 | CsWAT1-11 | 374 | 41,491.28 | 8.90 | 0.367 | 33.12 | No |
CsaV3_2G018040 | CsWAT1-12 | 314 | 34,780.27 | 9.07 | 0.596 | 36.94 | No |
CsaV3_2G029170 | CsWAT1-13 | 396 | 42,845.51 | 8.95 | 0.591 | 35.73 | No |
CsaV3_3G002650 | CsWAT1-14 | 342 | 37,246.73 | 9.03 | 0.842 | 39.66 | No |
CsaV3_3G039950 | CsWAT1-15 | 397 | 43,687.78 | 9.13 | 0.534 | 29.42 | No |
CsaV3_3G039960 | CsWAT1-16 | 305 | 33,692.49 | 6.93 | 0.372 | 32.50 | No |
CsaV3_3G039970 | CsWAT1-17 | 387 | 42,729.21 | 9.37 | 0.669 | 36.12 | No |
CsaV3_3G039980 | CsWAT1-18 | 381 | 42,023.38 | 9.36 | 0.728 | 33.98 | No |
CsaV3_3G042150 | CsWAT1-19 | 349 | 37,839.84 | 8.89 | 0.724 | 31.54 | No |
CsaV3_3G042160 | CsWAT1-20 | 356 | 38,697.67 | 8.35 | 0.650 | 33.29 | No |
CsaV3_3G042170 | CsWAT1-21 | 361 | 39,111.1 | 8.34 | 0.672 | 37.53 | No |
CsaV3_3G045560 | CsWAT1-22 | 394 | 42,528.02 | 9.23 | 0.640 | 33.71 | No |
CsaV3_4G024710 | CsWAT1-23 | 375 | 41,352.42 | 9.23 | 0.547 | 32.18 | No |
CsaV3_4G027990 | CsWAT1-24 | 374 | 40,087.27 | 8.50 | 0.699 | 30.19 | No |
CsaV3_4G028340 | CsWAT1-25 | 374 | 40,756.59 | 9.30 | 0.670 | 27.13 | No |
CsaV3_4G029290 | CsWAT1-26 | 441 | 49,378.12 | 8.94 | 0.350 | 35.64 | No |
CsaV3_5G001460 | CsWAT1-27 | 362 | 39,127.52 | 8.77 | 0.702 | 30.82 | No |
CsaV3_5G004250 | CsWAT1-28 | 360 | 38,969.85 | 9.02 | 0.851 | 37.27 | No |
CsaV3_5G006960 | CsWAT1-29 | 308 | 34,086.16 | 9.66 | 0.308 | 34.56 | No |
CsaV3_5G007970 | CsWAT1-30 | 377 | 41,435.33 | 8.99 | 0.627 | 36.52 | No |
CsaV3_5G026330 | CsWAT1-31 | 376 | 40,929.42 | 9.38 | 0.522 | 26.25 | No |
CsaV3_5G026340 | CsWAT1-32 | 355 | 39,086.41 | 9.43 | 0.622 | 24.44 | No |
CsaV3_5G026350 | CsWAT1-33 | 357 | 39,279.63 | 9.51 | 0.65 | 30.04 | No |
CsaV3_5G026360 | CsWAT1-34 | 374 | 40,691.15 | 9.56 | 0.471 | 30.09 | No |
CsaV3_5G029570 | CsWAT1-35 | 406 | 45,391.56 | 9.35 | 0.365 | 32.63 | No |
CsaV3_5G031760 | CsWAT1-36 | 374 | 41,277.68 | 8.62 | 0.601 | 34.41 | No |
CsaV3_5G037340 | CsWAT1-37 | 348 | 38,209.47 | 9.16 | 0.778 | 33.58 | No |
CsaV3_5G037350 | CsWAT1-38 | 377 | 41,006.99 | 8.25 | 0.492 | 40.00 | No |
CsaV3_6G005240 | CsWAT1-39 | 365 | 41,024.8 | 9.57 | 0.502 | 25.22 | No |
CsaV3_6G013640 | CsWAT1-40 | 332 | 37,085.6 | 8.98 | -0.714 | 56.11 | No |
CsaV3_6G041910 | CsWAT1-41 | 417 | 46,071.84 | 9.48 | 0.463 | 31.34 | No |
CsaV3_6G041920 | CsWAT1-42 | 277 | 31,060.88 | 9.75 | 0.442 | 42.34 | No |
CsaV3_6G041930 | CsWAT1-43 | 288 | 31,413.17 | 9.46 | 0.639 | 30.95 | No |
CsaV3_6G041950 | CsWAT1-44 | 360 | 39,766.27 | 9.45 | 0.606 | 31.37 | No |
CsaV3_6G041960 | CsWAT1-45 | 425 | 47,743.04 | 9.59 | 0.505 | 30.60 | No |
CsaV3_6G041970 | CsWAT1-46 | 273 | 30,516.62 | 9.26 | 0.606 | 30.32 | No |
CsaV3_6G041980 | CsWAT1-47 | 362 | 39,794.29 | 8.96 | 0.581 | 31.84 | No |
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Hong, J.; Zhao, H.; Yuan, Y.; Wu, J.; Yu, Y.; Cui, N.; Meng, X.; Fan, H. Characterization of Walls Are Thin 1 Family in Cucumis sativus and Functional Identification of CsWAT1-20 in Response to Podosphaera xanthii. Horticulturae 2025, 11, 620. https://doi.org/10.3390/horticulturae11060620
Hong J, Zhao H, Yuan Y, Wu J, Yu Y, Cui N, Meng X, Fan H. Characterization of Walls Are Thin 1 Family in Cucumis sativus and Functional Identification of CsWAT1-20 in Response to Podosphaera xanthii. Horticulturae. 2025; 11(6):620. https://doi.org/10.3390/horticulturae11060620
Chicago/Turabian StyleHong, Jinghang, Hongyan Zhao, Youmei Yuan, Jinming Wu, Yang Yu, Na Cui, Xiangnan Meng, and Haiyan Fan. 2025. "Characterization of Walls Are Thin 1 Family in Cucumis sativus and Functional Identification of CsWAT1-20 in Response to Podosphaera xanthii" Horticulturae 11, no. 6: 620. https://doi.org/10.3390/horticulturae11060620
APA StyleHong, J., Zhao, H., Yuan, Y., Wu, J., Yu, Y., Cui, N., Meng, X., & Fan, H. (2025). Characterization of Walls Are Thin 1 Family in Cucumis sativus and Functional Identification of CsWAT1-20 in Response to Podosphaera xanthii. Horticulturae, 11(6), 620. https://doi.org/10.3390/horticulturae11060620