Next Article in Journal
Comparative Transcriptome Analysis Reveals Key miRNAs and Pathways Involved in Adventitious Root Formation in Peach
Previous Article in Journal
Effect of Plant Growth-Promoting Rhizobacteria Inoculation on Sweet Potato Yield and Nutritional Quality in Northeast Thailand
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Horticulturae
Volume 11
Issue 12
10.3390/horticulturae11121443
horticulturae-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Fan-miR159 Family Targets Two Types of Genes to Potentially Regulate the Development of Strawberry

by
Xiaotong Jing
1,2,3,
Xinjia Huai
3,
Chuanli Ning
4,
Quan Zou
1,
Qi An
3,
Ejiao Wu
2,3,
Huazhao Yuan
2,3,
Jiahui Liang
2,3,* and
Yushan Qiao
2,3,*
1
Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 610054, China
2
Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Pomology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
3
Zhongshan Biological Breeding Laboratory, Nanjing 210014, China
4
Yantai Agricultural Technology Extension Center, Yantai 264001, China
*
Authors to whom correspondence should be addressed.
Horticulturae 2025, 11(12), 1443; https://doi.org/10.3390/horticulturae11121443 (registering DOI)
Submission received: 23 October 2025 / Revised: 21 November 2025 / Accepted: 26 November 2025 / Published: 28 November 2025
(This article belongs to the Section Developmental Physiology, Biochemistry, and Molecular Biology)
Versions Notes

Abstract

MicroRNAs (miRNAs) represent the most abundant class of small RNAs in plants, in which the miR159 family demonstrates a high degree of conservation across various plant species and plays a crucial role in regulating anther, silique, and seed development by targeting GAMYB-like. The members of the Fan-miR159 family in cultivated strawberry target not only GAMYB-like genes but also Auxin Response Factor (ARF) family members, which are annotated as “ARF23-like” in the genome. In this study, we firstly analyzed the structural features, evolutionary conservation, and expression patterns of the miR159 family in Fragaria × ananassa. Then, we reannotated the “ARF23-like” genes in cultivated strawberry and found that they actually belong to the “ARF2b” subfamily. Finally, we utilized RT-qPCR and co-transformation experiments to analyze and validate our observation. The results showed the suppression of FaARF2b-2 expression by fan-miR159n and fan-miR159r in both tobacco and strawberry. Moreover, two transcripts of the FaARF2b-2 gene were detected in cultivated strawberry, FaARF2b-2R and FaARF2b-2M. The expression levels of both FaARF2b-2M and FaARF2b-2R increased following the abscisic acid (ABA) treatment, indicating that they may be positively regulated by ABA. In addition, FaARF2b-2M exhibits significantly higher expression levels in the anther compared to FaARF2b-2R, which potentially plays an important role in the male reproductive development. Our findings enhance the understanding of the miR159 family in cultivated strawberry and expand the knowledge regarding its novel targets.
Keywords: miR159; ARF; target genes; strawberry miR159; ARF; target genes; strawberry

Share and Cite

MDPI and ACS Style

Jing, X.; Huai, X.; Ning, C.; Zou, Q.; An, Q.; Wu, E.; Yuan, H.; Liang, J.; Qiao, Y. Fan-miR159 Family Targets Two Types of Genes to Potentially Regulate the Development of Strawberry. Horticulturae 2025, 11, 1443. https://doi.org/10.3390/horticulturae11121443

AMA Style

Jing X, Huai X, Ning C, Zou Q, An Q, Wu E, Yuan H, Liang J, Qiao Y. Fan-miR159 Family Targets Two Types of Genes to Potentially Regulate the Development of Strawberry. Horticulturae. 2025; 11(12):1443. https://doi.org/10.3390/horticulturae11121443

Chicago/Turabian Style

Jing, Xiaotong, Xinjia Huai, Chuanli Ning, Quan Zou, Qi An, Ejiao Wu, Huazhao Yuan, Jiahui Liang, and Yushan Qiao. 2025. "Fan-miR159 Family Targets Two Types of Genes to Potentially Regulate the Development of Strawberry" Horticulturae 11, no. 12: 1443. https://doi.org/10.3390/horticulturae11121443

APA Style

Jing, X., Huai, X., Ning, C., Zou, Q., An, Q., Wu, E., Yuan, H., Liang, J., & Qiao, Y. (2025). Fan-miR159 Family Targets Two Types of Genes to Potentially Regulate the Development of Strawberry. Horticulturae, 11(12), 1443. https://doi.org/10.3390/horticulturae11121443

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop