Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

Dear author,

Thanks for your good report. My concern and questions regarding your manuscript are in the attached file.

Best regards

Comments for author File: Comments.pdf

Author Response

Please see the attachment. Thank you.

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

The submitted manuscript is an interesting material that talks about changes in polyphenols during storage in conditions of increased oxygen content. It is clearly written and relevant literature is presented. My attention is primarily devoted to the chemical part, namely the analysis of polyphenolic components.

In part 2.2.1. which the authors named "Polyphenols Concentration" give quantification as the total content of tannins relative to anthocyanins. The authors state "Values ​​for tannins and flavones were expressed as proanthocyanidin tetramer equivalents (mg/L), and the values ​​for anthocyanin contents were expressed as cyanidin-3-glucoside (mg/L)". In the experimental part, they would have to state which standards they used (manufacturer and quality). Why did they decide to express flavones via proanthocyanidin tetramer?

Identification of components was done by mass spectrometry. Table 1 lists the retention time, molecular ion m/z and Main fragment. <I ask the authors to explain how they identified the listed compounds in this way.

Strawberries are rich in ellagic acid and ellagitannins. These compounds are not listed in the results. What was the reason

Author Response

Please see the attachment. Thank you.

Author Response File: Author Response.pdf

Reviewer 3 Report

Comments and Suggestions for Authors

The manuscript “Elevated Oxygen Contributes to Promotion of Polyphenols Biosynthesis and Antioxidant Capacity: A Case Study in Strawberry” deal with the impact of different concentrations of oxygen on polyphenol metabolism, individual polyphenol contents, and antioxidant levels of strawberries during storage. Additionally, most genes involved in polyphenols biosynthesis were found to be upregulated during storage, with elevated oxygen resulting in higher expression levels compared with controls.

Although it is clear that much effort was put into the creation of this manuscript, the presentation of the results was inadequate.

The first drawback, which extends through all parts of the manuscript, is the inappropriate way in which bibliographical references are given. It is impossible for the reader to follow and verify the goodness of these references.

Page 2, line 76 and 77 are unclear

In the materials and methods section, it is necessary to write all the standards that were used and their purity. Furthermore, sections 2.2 and 2.4 should be developed with more information.

The authors reported the polyphenols concentration and the concentration of the individual compounds in the figure 3B, but the procedure for the qualitative and quantitative analysis of these components is not provided.

Figure 3A (top) reported the chromatograms of polyphenols at 280 nm. Some absorptions were not considered (at 13.5 min. and 15 min.). Have other wavelengths been considered for a more complete characterization?

Most of the figures are difficult to understand and visible (Fig. 5 in particular)

Author Response

Please see the attachment. Thank you.

Author Response File: Author Response.pdf

Reviewer 4 Report

Comments and Suggestions for Authors

Review of MS: Elevated oxygen contributes to promotion of polyphenols biosynthesis and antioxidant capacity: A case study in Strawberry.

This paper presents the effect of high oxygen concentrations in the atmosphere of stored strawberries on the course of their coloration and the content of polyphenols, divided into tannins, anthocyanins and flavones.

There are comments and questions, which I include below, and others directly in the text

1.      Was only one experiment conducted or was it repeated? If the experiment is repeated and the same trends in response to a given factor are observed, then the conclusions are more reliable.

2.      The citations in the paper are marked in such a way that they cannot be related to the items in the References section.

3.      Some of the results shown in the figures are illegible due to the font being too small and insufficient descriptions of the figures.

4.      Other comments are posted directly on MS

Comments for author File: Comments.pdf

Author Response

Please see the attachment. Thank you.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Comments and Suggestions for Authors

Dear authors,

Thanks for the revised paper. Please mention it in methods that you measured CO2 and high O2, not N2. Also, add type of boxes and its permeability to gases.

Best regards

Author Response

Please see the attachment. Thank you.

Author Response File: Author Response.pdf

Reviewer 3 Report

Comments and Suggestions for Authors

The authors of manuscript “Elevated Oxygen Contributes to Promotion of Polyphenols Biosynthesis and Antioxidant Capacity: A Case Study in Strawberry” proposed a new version of the manuscript.

In the new version, many corrections have been made as requested.

Nevertheless, the authors described the concentration of the individual compounds in the figure 3B, but the procedure for the quantitative analysis of these components is not clearly reported.

Furthermore, the bibliography should be reported in the format suggested by horticulturae (MDPI)

Author Response

Please see the attachment. Thank you.

Author Response File: Author Response.pdf

Reviewer 4 Report

Comments and Suggestions for Authors

I have included comments and suggestions directly in the text

Comments for author File: Comments.pdf

Author Response

Please see the attachment.Thank you.

Author Response File: Author Response.pdf