Genome-Wide Identification of the ARF Gene Family in Three Dendrobium Species and Its Expression Pattern Analysis in D. nobile Flower

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

This study aimed to explore ARF family genes in the genomes of three Orchidaceae species—D. chrysotoxum, D. huoshanense, and D. nobile—using bioinformatic and transcriptomic tools. The results provide insights into the potential functions of ARF genes in these Dendrobium species and suggest avenues for further exploration of their roles and regulatory mechanisms during flower development.

The manuscript is well-structured and well-written, and the methodology is appropriate for this study. I recommend accepting the manuscript after the authors make some improvements.

The statement "Altogether, our study results provide comprehensive data to elucidate the potential functions of ARF genes in three Dendrobium species and suggest new insights for further exploration concerning the function and regulatory mechanisms in Dendrobium during flower development" appears twice in the abstract and the introduction. Please reformulate one of these instances to avoid redundancy.

In the Expression Analysis section, the authors did not provide information about the characteristics of the plant material used (such as varieties, accessions, origin, or identification).

Details on the experimental design and the culture conditions of the investigated plant material are also missing.

Additionally, the time of plant material harvest should be indicated (time after initial culture). Information on whether the studied species were propagated through cuttings or seeds is also required.

All these details are essential for a comprehensive understanding of the study.

Author Response

Dear reviewer

Thank you very much for your thoughtful and detailed comments on our manuscript (horticulturae-3016539). We appreciate the time and effort you have taken to review our work and provide constructive feedback.

In response to your comments, we have made the following revisions:

Comment 1. The statement "Altogether, our study results provide comprehensive data to elucidate the potential functions of ARF genes in three Dendrobium species and suggest new insights for further exploration concerning the function and regulatory mechanisms in Dendrobium during flower development" appears twice in the abstract and the introduction. Please reformulate one of these instances to avoid redundancy.

Response: Thanks for your comment. We have revised this sentence, in introduction section, please see page2, lines 88–91.

     

Comment 2. In the Expression Analysis section, the authors did not provide information about the characteristics of the plant material used (such as varieties, accessions, origin, or identification). Details on the experimental design and the culture conditions of the investigated plant material are also missing. Additionally, the time of plant material harvest should be indicated (time after initial culture). Information on whether the studied species were propagated through cuttings or seeds is also required. All these details are essential for a comprehensive understanding of the study.

Response: Thanks for your comment. We have added some information about the plant material used, please see page 4, lines 150–153.

We believe that these revisions have significantly improved the quality of our manuscript. We are grateful for your insights and recommendations.

Best regards, Siren Lan

 

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

Zhang and collaborators present a nice work about genome-wide identification of ARF gene family in several Dendrobium orchids. The document submitted is well-structured and written. Although the authors made a compressive wide-genome study, they can do concise and robust discussion and conclusions according to the results. At this point, I have only minor issues:

 

-Maybe I got lost during the reading. However, I couldn't find why they selected D. nobile to evaluate some gene expression patterns experimentally.

-Line 69: Declare before using the following scientific name: Ap. shenzhenica

-Line 240: why did you use capital letters for the word motif in this sentence?

-Line 297: Figure 5 right side: The bottom legends are unreadable.

Author Response

Dear reviewer

Thank you very much for your thoughtful and detailed comments on our manuscript (horticulturae-3016539). We appreciate the time and effort you have taken to review our work and provide constructive feedback.

Comment 1. Maybe I got lost during the reading. However, I couldn't find why they selected D. nobile to evaluate some gene expression patterns experimentally.

Response: Thanks for your comment. We have revised the problem, please see page 2, lines 84–86, and page 12, line 340.

Comment 2. Line 69: Declare before using the following scientific name: Ap. shenzhenica.

Response: Thanks for your comment. We have changed “Ap. shenzhenica” to “Apostasia shenzhenica”, please see page 2, lines 69-70.

Comment 3. Line 240: why did you use capital letters for the word motif in this sentence?

Response: Thank you. We have changed “Motif” to “motif”, Please see page 9, lines 244.

Comment 4. Line 297: Figure 5 right side: The bottom legends are unreadable.

Response: Thanks for your comment. We have revised the legend of Figure 5, please see page 11, line 300.

We believe that these revisions have significantly improved the quality of our manuscript. We are grateful for your insights and recommendations.

Best regards,

Siren Lan

 

 

 

Author Response File: Author Response.pdf