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Volume 9
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Review

Advances in Synthetic Biology Techniques and Industrial Applications of Corynebacterium glutamicum

by
Yujue Wang
1,2,†,
Qiang Wang
1,2,†,
Aobo Sha
1,2,
Kexin Ren
1,2,
Mengkai Hu
1,2,
Meijuan Xu
1,2,
Xian Zhang
1,2,* and
Zhiming Rao
1,2
1
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214126, China
2
Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Fermentation 2023, 9(8), 729; https://doi.org/10.3390/fermentation9080729
Submission received: 3 July 2023 / Revised: 24 July 2023 / Accepted: 1 August 2023 / Published: 4 August 2023
(This article belongs to the Special Issue New Insights into Amino Acid Biosynthesis)
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Abstract

Corynebacterium glutamicum is a Gram-positive bacterium (non-spore-forming) that has been wildly used for amino acid production. Due to its stable protein secretion, low extracellular hydrolase activity, and non-toxicity, the application field of C. glutamicum has been greatly expanded. Currently, gene editing technology based on synthetic biology has great potential for synthetic biology research and genetic modification in C. glutamicum because of its ability to efficiently regulate the physiological and metabolic networks of the strain. Therefore, we summarize the gene editing tools and strategies of C. glutamicum from the aspects of genetic modification and expression elements, and we also describe the effects of gene editing techniques on a variety of products such as amino acids and their derivatives, recombinant proteins, and functional sugars, which provide a certain theoretical basis for the research on the modification of C. glutamicum strains and industrial applications. Finally, we prospect the design and industrial application of C. glutamicum genetic modification from multiple perspectives based on gene editing techniques.
Keywords: Corynebacterium glutamicum; genetic modification; gene editing techniques; expression elements; application Corynebacterium glutamicum; genetic modification; gene editing techniques; expression elements; application

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MDPI and ACS Style

Wang, Y.; Wang, Q.; Sha, A.; Ren, K.; Hu, M.; Xu, M.; Zhang, X.; Rao, Z. Advances in Synthetic Biology Techniques and Industrial Applications of Corynebacterium glutamicum. Fermentation 2023, 9, 729. https://doi.org/10.3390/fermentation9080729

AMA Style

Wang Y, Wang Q, Sha A, Ren K, Hu M, Xu M, Zhang X, Rao Z. Advances in Synthetic Biology Techniques and Industrial Applications of Corynebacterium glutamicum. Fermentation. 2023; 9(8):729. https://doi.org/10.3390/fermentation9080729

Chicago/Turabian Style

Wang, Yujue, Qiang Wang, Aobo Sha, Kexin Ren, Mengkai Hu, Meijuan Xu, Xian Zhang, and Zhiming Rao. 2023. "Advances in Synthetic Biology Techniques and Industrial Applications of Corynebacterium glutamicum" Fermentation 9, no. 8: 729. https://doi.org/10.3390/fermentation9080729

APA Style

Wang, Y., Wang, Q., Sha, A., Ren, K., Hu, M., Xu, M., Zhang, X., & Rao, Z. (2023). Advances in Synthetic Biology Techniques and Industrial Applications of Corynebacterium glutamicum. Fermentation, 9(8), 729. https://doi.org/10.3390/fermentation9080729

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