Selection of Yeast and Lactic Acid Bacteria Strains, Isolated from Spontaneous Raw Milk Fermentation, for the Production of a Potential Probiotic Fermented Milk
Abstract
:1. Introduction
2. Materials and Methods
2.1. Raw Milk Spontaneous Fermentation
2.2. Microorganism Enumeration
2.3. Identification and Typing of Yeasts
2.4. Microorganisms and Culture Condition
2.5. Yeast and Lactic Acid Bacteria Probiotic Features Assessment
2.5.1. Bile Salt Tolerance
2.5.2. Tolerance to Acidic Condition
2.6. Lactose Utilization
2.7. Resistance to Simulated Human Gastrointestinal (GI) Conditions
2.8. Manufacture of Fermented Milk and Microbial Viability during Storage
2.9. Resistance to Simulated Gastrointestinal Conditions of Microorganisms in Fermented Milk
2.10. Organic Acid Determination by High-Pressure Liquid Chromatography (HPLC)
2.11. Statistical Analysis
3. Results and Discussion
3.1. Raw Cow Milk Spontaneous Fermentation and Yeast Identification
3.2. Yeast Screening for Probiotic Features
3.3. Resistance of Yeasts to Simulated Gastrointestinal (GI) Conditions
3.4. Lactic Acid Bacteria Screening for Probiotic Features
3.5. Resistance of Lactic Acid Bacteria to Simulated Gastrointestinal Conditions
3.6. Fermented Milk Production
3.7. Microbial Survival during Simulated Gastrointestinal Conditions in Fermented Milk
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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FM-S | FM-B | |
---|---|---|
Lactic acid bacteria (CFU/mL) | (2.44 ± 0.69) × 109 a | (1.42 ± 0.44) × 109 a |
Yeasts (CFU/mL) | (2.14 ± 0.55) × 107 a | (2.76 ± 0.34) × 107 a |
Final pH | 4.21 ± 0.17 b | 4.59 ± 0.01 a |
ΔpH | −2.22 ± 0.11 b | −1.63 ± 0.04 a |
Lactose (g/L) | 31.75 ± 0.64 | 31.85 ± 0.64 |
Lactic acid (g/L) | 7.00 ± 0.49 b | 5.00 ± 0.26 a |
Acetic acid (g/L) | 0.58 ± 0.05 b | 0.38 ± 0.10 a |
Ethanol (v/v %) | 0.61 ± 0.06 a | 0.80 ± 0.07 b |
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Galli, V.; Venturi, M.; Mari, E.; Guerrini, S.; Granchi, L. Selection of Yeast and Lactic Acid Bacteria Strains, Isolated from Spontaneous Raw Milk Fermentation, for the Production of a Potential Probiotic Fermented Milk. Fermentation 2022, 8, 407. https://doi.org/10.3390/fermentation8080407
Galli V, Venturi M, Mari E, Guerrini S, Granchi L. Selection of Yeast and Lactic Acid Bacteria Strains, Isolated from Spontaneous Raw Milk Fermentation, for the Production of a Potential Probiotic Fermented Milk. Fermentation. 2022; 8(8):407. https://doi.org/10.3390/fermentation8080407
Chicago/Turabian StyleGalli, Viola, Manuel Venturi, Eleonora Mari, Simona Guerrini, and Lisa Granchi. 2022. "Selection of Yeast and Lactic Acid Bacteria Strains, Isolated from Spontaneous Raw Milk Fermentation, for the Production of a Potential Probiotic Fermented Milk" Fermentation 8, no. 8: 407. https://doi.org/10.3390/fermentation8080407