Nitrification upon Nitrogen Starvation and Recovery: Effect of Stress Period, Substrate Concentration and pH on Ammonia Oxidizers’ Performance
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Enrichment of Nitrifying Microorganisms
2.2. Experimental Setup
2.3. Analytical Methods and Statistical Analysis
2.4. DNA Extraction and Sequencing Data Analysis
3. Results and Discussion
3.1. Microbial Analysis of the Culture
3.2. The Effect of Short-Term Starvation and Starvation Period on Substrate Uptake
3.3. The Effect of Substrate Concentration on Nitrogen Removal by Starved Cells
3.4. The Effect of pH on Substrate Uptake by Starved Cells
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Abbaszadeh, L.; Koutra, E.; Tsigkou, K.; Gaspari, M.; Kougias, P.G.; Kornaros, M. Nitrification upon Nitrogen Starvation and Recovery: Effect of Stress Period, Substrate Concentration and pH on Ammonia Oxidizers’ Performance. Fermentation 2022, 8, 387. https://doi.org/10.3390/fermentation8080387
Abbaszadeh L, Koutra E, Tsigkou K, Gaspari M, Kougias PG, Kornaros M. Nitrification upon Nitrogen Starvation and Recovery: Effect of Stress Period, Substrate Concentration and pH on Ammonia Oxidizers’ Performance. Fermentation. 2022; 8(8):387. https://doi.org/10.3390/fermentation8080387
Chicago/Turabian StyleAbbaszadeh, Leila, Eleni Koutra, Konstantina Tsigkou, Maria Gaspari, Panagiotis G. Kougias, and Michael Kornaros. 2022. "Nitrification upon Nitrogen Starvation and Recovery: Effect of Stress Period, Substrate Concentration and pH on Ammonia Oxidizers’ Performance" Fermentation 8, no. 8: 387. https://doi.org/10.3390/fermentation8080387