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Open AccessArticle

Basidiomycotic Yeast Cryptococcus diffluens Converts l-Galactonic Acid to the Compound on the Similar Metabolic Pathway in Ascomycetes

Department of Applied Life Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, Japan
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Fermentation 2019, 5(3), 73; https://doi.org/10.3390/fermentation5030073
Received: 12 June 2019 / Revised: 26 July 2019 / Accepted: 3 August 2019 / Published: 5 August 2019
(1) Background: It has been shown that d-galacturonic acid is converted to l-galactonic acid by the basidiomycotic yeast, Cryptococcus diffluens. However, two pathways are hypothesized for the l-galactonic acid conversion process in C. diffluens. One is similar to the conversion process of the filamentous fungi in d-galacturonic acid metabolism and another is the conversion process to l-ascorbic acid, reported in the related yeast, C. laurentii. It is necessary to determine which, if either, process occurs in C. diffluens in order to produce novel value-added products from d-galacturonic acid using yeast strains. (2) Methods: The diethylaminoethy (DEAE)-fractionated enzyme was prepared from the cell-free extract of C. diffluens by the DEAE column chromatography. The l-galactonic acid conversion activity was assayed using DEAE-fractionated enzyme and the converted product was detected and fractionated by high-performance anion-exchange chromatography. Then, the molecular structure was identified by nuclear magnetic resonance analysis. (3) Results: The product showed similar chemical properties to 2-keto-3-deoxy-l-galactonic acid (l-threo-3-deoxy-hexulosonic acid). (4) Conclusions: It is suggested that l-galactonic acid is converted to 2-keto-3-deoxy-l-galactonic acid by dehydratase in C. diffluens. The l-galactonic acid conversion process of C. diffluens is a prioritized pathway, similar to the pathway of ascomycetes. View Full-Text
Keywords: Cryptococcus diffluens; basidiomycotic yeast; l-galactonic acid; dehydratase; 2-keto-3-deoxy-l-galactonic acid; d-galacturonic acid; NMR Cryptococcus diffluens; basidiomycotic yeast; l-galactonic acid; dehydratase; 2-keto-3-deoxy-l-galactonic acid; d-galacturonic acid; NMR
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MDPI and ACS Style

Matsubara, T.; Kataoka, M.; Kishida, M. Basidiomycotic Yeast Cryptococcus diffluens Converts l-Galactonic Acid to the Compound on the Similar Metabolic Pathway in Ascomycetes. Fermentation 2019, 5, 73.

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