The Use of UV-Vis Spectroscopy in Bioprocess and Fermentation Monitoring
Abstract
:1. Introduction
2. The Origin of the UV-Vis Spectra
3. Sample Presentation
4. Data Analysis
5. Applications and Examples of UV-Vis Spectroscopy in Bioprocess and Fermentation Monitoring
6. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Item | Advantages | Limitations |
---|---|---|
Samples and sampling | Allow the continuous sampling of the process | The sample and other interferences (e.g., damage cells, turbidity) are also analysed |
Hardware | Commercially available instrumentation easily available | Type of instrument, fibre optic options, highly dependent on the type of sample (e.g., liquid, semisolid, turbid media). |
Routine use | Easy to implement | Highly dependent on the type of sample, and process. |
Data analysis | A lot of information and data can be collected to monitor the process | Not only information about the sample is collected, interferences, noise is also collected during the process. |
Training | Easy to use in routine | Education and high understanding of the system, interpretation of the data and interferences. |
Chemical compounds and properties | Several compounds can be measured | Limit of detection and quantification, depending on the process and sample. |
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Roberts, J.; Power, A.; Chapman, J.; Chandra, S.; Cozzolino, D. The Use of UV-Vis Spectroscopy in Bioprocess and Fermentation Monitoring. Fermentation 2018, 4, 18. https://doi.org/10.3390/fermentation4010018
Roberts J, Power A, Chapman J, Chandra S, Cozzolino D. The Use of UV-Vis Spectroscopy in Bioprocess and Fermentation Monitoring. Fermentation. 2018; 4(1):18. https://doi.org/10.3390/fermentation4010018
Chicago/Turabian StyleRoberts, Jessica, Aoife Power, James Chapman, Shaneel Chandra, and Daniel Cozzolino. 2018. "The Use of UV-Vis Spectroscopy in Bioprocess and Fermentation Monitoring" Fermentation 4, no. 1: 18. https://doi.org/10.3390/fermentation4010018